1cfj - Revision history

OCA at 05:51, 9 August 2023

OCA — Wed, 09 Aug 2023 05:51:21 GMT

←Older revision		Revision as of 05:51, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='1cfj' size='340' side='right'caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1cfj' size='340' side='right'caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Torpedo_californica Torpedo~~ californica]. The June 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica]. The June 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>~~, ~~<scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [https://pdbe.org/1cfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [https://www.ebi.ac.uk/pdbsum/1cfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [https://pdbe.org/1cfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [https://www.ebi.ac.uk/pdbsum/1cfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~ACES_TORCA ACES_TORCA]~~] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.	+	[https://www.uniprot.org/uniprot/ACES_TETCF ACES_TETCF] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 39:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: ~~Torpedo~~ californica]]	+	[[Category: Tetronarce californica]]
-	[[Category: Millard~~, C B~~]]	+	[[Category: Millard CB]]
-	[[Category: Silman, I]]	+	[[Category: Silman I]]
-	[[Category: Sussman~~, J L]]~~	+	[[Category: Sussman JL]]
-	~~[[Category: Chemical-warfare]]~~	+
-	~~[[Category: Cholinesterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Organophosphate]]~~	+
-	~~[[Category: Serine hydrolase~~]]	+

OCA at 15:09, 2 June 2021

OCA — Wed, 02 Jun 2021 15:09:33 GMT

←Older revision		Revision as of 15:09, 2 June 2021
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	==METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)==		==METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)==
-	<StructureSection load='1cfj' size='340' side='right' caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='1cfj' size='340' side='right'caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG~~</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [~~http~~://pdbe.org/1cfj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cfj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [https://pdbe.org/1cfj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [https://www.ebi.ac.uk/pdbsum/1cfj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACES_TORCA ACES_TORCA]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.	+	[[https://www.uniprot.org/uniprot/ACES_TORCA ACES_TORCA]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[AChE inhibitors and substrates\|AChE inhibitors and substrates]]
	*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]		*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]
-	*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]
-	*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]
-	*[[3D structures of acetylcholinesterase\|3D structures of acetylcholinesterase]]
	== References ==		== References ==
	<references/>		<references/>
Line 41:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Torpedo californica]]		[[Category: Torpedo californica]]

OCA at 08:14, 29 November 2017

OCA — Wed, 29 Nov 2017 08:14:27 GMT

←Older revision		Revision as of 08:14, 29 November 2017
Line 1:		Line 1:
		+
	==METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)==		==METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)==
	<StructureSection load='1cfj' size='340' side='right' caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1cfj' size='340' side='right' caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://pdbe.org/1cfj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://pdbe.org/1cfj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 32:
	==See Also==		==See Also==
	*[[AChE inhibitors and substrates\|AChE inhibitors and substrates]]		*[[AChE inhibitors and substrates\|AChE inhibitors and substrates]]
		+	*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]
	*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]		*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]
	*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]		*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]

OCA at 13:03, 9 February 2016

OCA — Tue, 09 Feb 2016 13:03:25 GMT

←Older revision		Revision as of 13:03, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cfj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[AChE inhibitors and substrates\|AChE inhibitors and substrates]]		*[[AChE inhibitors and substrates\|AChE inhibitors and substrates]]
-	*[[Acetylcholinesterase\|Acetylcholinesterase]]
	*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]		*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]
	*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]		*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]
		+	*[[3D structures of acetylcholinesterase\|3D structures of acetylcholinesterase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:31, 10 September 2015

OCA — Thu, 10 Sep 2015 22:31:50 GMT

←Older revision		Revision as of 22:31, 10 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://pdbe.org/1cfj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cfj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:59, 25 December 2014

OCA — Thu, 25 Dec 2014 06:59:28 GMT

←Older revision		Revision as of 06:59, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACES_TORCA ACES_TORCA]] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:46, 22 December 2014

OCA — Mon, 22 Dec 2014 08:46:07 GMT

←Older revision		Revision as of 08:46, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 38:
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Torpedo californica]]		[[Category: Torpedo californica]]
-	[[Category: Millard, C B.]]	+	[[Category: Millard, C B]]
-	[[Category: Silman, I.]]	+	[[Category: Silman, I]]
-	[[Category: Sussman, J L.]]	+	[[Category: Sussman, J L]]
	[[Category: Chemical-warfare]]		[[Category: Chemical-warfare]]
	[[Category: Cholinesterase]]		[[Category: Cholinesterase]]

OCA at 14:00, 29 September 2014

OCA — Mon, 29 Sep 2014 14:00:40 GMT

←Older revision		Revision as of 14:00, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1cfj\| PDB=1cfj \| SCENE= }}~~	+	==METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)==
-	===METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)===	+	<StructureSection load='1cfj' size='340' side='right' caption='[[1cfj]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_10353814}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GB:METHYLPHOSPHONIC+ACID+ESTER+GROUP'>GB</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetylcholinesterase Acetylcholinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.7 3.1.1.7] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfj RCSB], [http://www.ebi.ac.uk/pdbsum/1cfj PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Organophosphorus acid anhydride (OP) nerve agents are potent inhibitors which rapidly phosphonylate acetylcholinesterase (AChE) and then may undergo an internal dealkylation reaction (called "aging") to produce an OP-enzyme conjugate that cannot be reactivated. To understand the basis for irreversible inhibition, we solved the structures of aged conjugates obtained by reaction of Torpedo californica AChE (TcAChE) with diisopropylphosphorofluoridate (DFP), O-isopropylmethylphosponofluoridate (sarin), or O-pinacolylmethylphosphonofluoridate (soman) by X-ray crystallography to 2.3, 2.6, or 2.2 A resolution, respectively. The highest positive difference density peak corresponded to the OP phosphorus and was located within covalent bonding distance of the active-site serine (S200) in each structure. The OP-oxygen atoms were within hydrogen-bonding distance of four potential donors from catalytic subsites of the enzyme, suggesting that electrostatic forces significantly stabilize the aged enzyme. The active sites of aged sarin- and soman-TcAChE were essentially identical and provided structural models for the negatively charged, tetrahedral intermediate that occurs during deacylation with the natural substrate, acetylcholine. Phosphorylation with DFP caused an unexpected movement in the main chain of a loop that includes residues F288 and F290 of the TcAChE acyl pocket. This is the first major conformational change reported in the active site of any AChE-ligand complex, and it offers a structural explanation for the substrate selectivity of AChE.

-	~~==About this Structure==~~	+	Crystal structures of aged phosphonylated acetylcholinesterase: nerve agent reaction products at the atomic level.,Millard CB, Kryger G, Ordentlich A, Greenblatt HM, Harel M, Raves ML, Segall Y, Barak D, Shafferman A, Silman I, Sussman JL Biochemistry. 1999 Jun 1;38(22):7032-9. PMID:10353814<ref>PMID:10353814</ref>
-	~~[[1cfj]] is a 1 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Torpedo_californica Torpedo californica]~~. ~~The June 2004 RCSB PDB [http~~://~~pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule~~ of the ~~Month] feature on ''Acetylcholinesterase'' by David~~ S. ~~Goodsell is [http://dx~~.~~doi.org~~/~~10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
Line 11:		Line 31:
	*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]		*[[Acetylcholinesterase inhibited by nerve agent soman\|Acetylcholinesterase inhibited by nerve agent soman]]
	*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]		*[[Acetylcholinesterase with DFP\|Acetylcholinesterase with DFP]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010353814<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Acetylcholinesterase]]		[[Category: Acetylcholinesterase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]

OCA at 09:16, 11 March 2013

OCA — Mon, 11 Mar 2013 09:16:42 GMT

←Older revision		Revision as of 09:16, 11 March 2013
Line 1:		Line 1:
-	[[Image:1cfj.png\|left\|200px]]
-
	{{STRUCTURE_1cfj\| PDB=1cfj \| SCENE= }}		{{STRUCTURE_1cfj\| PDB=1cfj \| SCENE= }}
-
	===METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)===		===METHYLPHOSPHONYLATED ACETYLCHOLINESTERASE (AGED) OBTAINED BY REACTION WITH O-ISOPROPYLMETHYLPHOSPHONOFLUORIDATE (GB, SARIN)===
-
	{{ABSTRACT_PUBMED_10353814}}		{{ABSTRACT_PUBMED_10353814}}

OCA at 13:56, 20 October 2012

OCA — Sat, 20 Oct 2012 13:56:15 GMT

←Older revision		Revision as of 13:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1cfj]] is a 1 chain structure ~~of [[Acetylcholinesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA].	+	[[1cfj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. The June 2004 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Acetylcholinesterase'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2004_6 10.2210/rcsb_pdb/mom_2004_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFJ OCA].

	==See Also==		==See Also==