1cfv - Revision history

OCA at 05:51, 9 August 2023

OCA — Wed, 09 Aug 2023 05:51:28 GMT

←Older revision		Revision as of 05:51, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [https://pdbe.org/1cfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [https://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [https://pdbe.org/1cfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [https://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KV2A7_MOUSE KV2A7_MOUSE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Phillips~~, S E.V~~]]	+	[[Category: Phillips SEV]]
-	[[Category: Trinh~~, C H]]~~	+	[[Category: Trinh CH]]
-	~~[[Category: Fine specificity]]~~	+
-	~~[[Category: Fv fragment]]~~	+
-	~~[[Category: Immunoglobulin]]~~	+
-	~~[[Category: Steroid hormone~~]]	+

OCA at 09:50, 15 September 2021

OCA — Wed, 15 Sep 2021 09:50:07 GMT

←Older revision		Revision as of 09:50, 15 September 2021
Line 3:		Line 3:
	<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CFV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [~~http~~://pdbe.org/1cfv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [https://pdbe.org/1cfv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [https://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:28, 11 December 2019

OCA — Wed, 11 Dec 2019 15:28:55 GMT

←Older revision		Revision as of 15:28, 11 December 2019
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	<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://pdbe.org/1cfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://pdbe.org/1cfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H]]		[[Category: Trinh, C H]]

OCA at 06:51, 29 May 2019

OCA — Wed, 29 May 2019 06:51:43 GMT

←Older revision		Revision as of 06:51, 29 May 2019
Line 1:		Line 1:

	==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==		==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
-	<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1cfv' size='340' side='right'caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfv_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfv_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H]]		[[Category: Trinh, C H]]

OCA at 11:07, 24 August 2017

OCA — Thu, 24 Aug 2017 11:07:08 GMT

←Older revision		Revision as of 11:07, 24 August 2017
Line 1:		Line 1:
		+
	==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==		==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
	<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://pdbe.org/1cfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://pdbe.org/1cfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfv ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cfv ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:

	==See Also==		==See Also==
-	*[[~~Monoclonal Antibody~~\|~~Monoclonal Antibody~~]]	+	*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Lk3 transgenic mice]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H]]		[[Category: Trinh, C H]]

OCA at 11:09, 11 September 2015

OCA — Fri, 11 Sep 2015 11:09:12 GMT

←Older revision		Revision as of 11:09, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://pdbe.org/1cfv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cfv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibody\|Monoclonal Antibody]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Phillips, S E.V]]		[[Category: Phillips, S E.V]]
	[[Category: Trinh, C H]]		[[Category: Trinh, C H]]

OCA at 09:29, 22 December 2014

OCA — Mon, 22 Dec 2014 09:29:55 GMT

←Older revision		Revision as of 09:29, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Phillips, S E.V.]]	+	[[Category: Phillips, S E.V]]
-	[[Category: Trinh, C H.]]	+	[[Category: Trinh, C H]]
	[[Category: Fine specificity]]		[[Category: Fine specificity]]
	[[Category: Fv fragment]]		[[Category: Fv fragment]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Steroid hormone]]		[[Category: Steroid hormone]]

OCA at 16:55, 20 August 2014

OCA — Wed, 20 Aug 2014 16:55:34 GMT

←Older revision		Revision as of 16:55, 20 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cfv.~~png\|left~~\|200px]]	+	==MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI==
		+	<StructureSection load='1cfv' size='340' side='right' caption='[[1cfv]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CFV FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E3G:ESTRONE+BETA-D-GLUCURONIDE'>E3G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cfv RCSB], [http://www.ebi.ac.uk/pdbsum/1cfv PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfv_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The concentration of steroid glucuronides in serial samples of early morning urine (EMU) can be used to predict the fertile period in the female menstrual cycle. The monoclonal antibody 4155 has been used as a convenient means of measuring the concentration of steroid glucuronides in EMU, as it specifically recognises the steroid hormone estrone beta-D-glucuronide (E3G), with very high affinity, and the closely related hormone estriol 3-(beta-d-glucuronide) (EI3G), with reduced affinity. Although 4115 binds these hormones with different affinities, EI3G differs from E3G only in the addition of a hydroxyl group and reduction of an adjacent carbonyl. To investigate the structural basis of this fine binding specificity, we have determined the crystal structures of the variable fragment (Fv) of 4155 in complex with each of these hormones. RESULTS: Two crystal forms of the Fv4155-EI3G complex, at resolutions of 2.1 A and 2.5 A, and one form of the Fv4155-E3G complex, at 2.1 A resolution were solved and refined. The crystal structures show the E3G or EI3G antigen lying in an extended cleft, running form the centre of the antibody combining site down one side of the variable domain interface, and formed almost entirely from residues in the heavy chain. The binding cleft lies primarily between the heavy chain complementarity determining regions (CDRs), rather than in the interface between the heavy and light chains. In both complexes the binding of the glucuronic sugar, and rings A and B of the steroid, is specified by the shape of the narrow cleft. Analysis of the Fv structure reveals that five of the six CDR regions can be assigned to one of the predefined canonical structural classes. CONCLUSIONS: The difference in the binding affinity of Fv4155 for the two steroid hormones is accounted for by a subtle combination of a less favoured hydrogen-bond geometry, and a minor rearrangement of the water molecule network around the binding site. The rearrangement of water molecules results from the burial of the additional hydroxyl group of the EI3G in a hydrophobic environment.

-	~~{{STRUCTURE_1cfv\| PDB=1cfv \| SCENE= }}~~	+	Antibody fragment Fv4155 bound to two closely related steroid hormones: the structural basis of fine specificity.,Trinh CH, Hemmington SD, Verhoeyen ME, Phillips SE Structure. 1997 Jul 15;5(7):937-48. PMID:9261086<ref>PMID:9261086</ref>

-	~~===MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9261086}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1cfv]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009261086<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Phillips, S E.V.]]		[[Category: Phillips, S E.V.]]

OCA: Protected "1cfv" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 19:59:18 GMT

Protected "1cfv" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:59, 21 October 2012

OCA at 19:59, 21 October 2012

OCA — Sun, 21 Oct 2012 19:59:16 GMT

←Older revision		Revision as of 19:59, 21 October 2012
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-	{{Seed}}
	[[Image:1cfv.png\|left\|200px]]		[[Image:1cfv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1cfv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1cfv\| PDB=1cfv \| SCENE= }}		{{STRUCTURE_1cfv\| PDB=1cfv \| SCENE= }}

	===MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI===		===MONOCLONAL ANTIBODY FRAGMENT FV4155 FROM E. COLI===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9261086}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9261086 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9261086}}		{{ABSTRACT_PUBMED_9261086}}

	==About this Structure==		==About this Structure==
-	~~1CFV~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA].	+	[[1cfv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFV OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9261086~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009261086</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Phillips, S E.V.]]		[[Category: Phillips, S E.V.]]
Line 31:		Line 19:
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Steroid hormone]]		[[Category: Steroid hormone]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:39:48 2009''