1cij - Revision history

OCA at 05:52, 9 August 2023

OCA — Wed, 09 Aug 2023 05:52:24 GMT

←Older revision		Revision as of 05:52, 9 August 2023
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	<StructureSection load='1cij' size='340' side='right'caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cij' size='340' side='right'caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [https://pdbe.org/1cij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [https://www.ebi.ac.uk/pdbsum/1cij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cij ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [https://pdbe.org/1cij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [https://www.ebi.ac.uk/pdbsum/1cij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cij ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.	+	[https://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Corynebacterium autotrophicum baumgarten et al. 1974]]
-	[[Category: Haloalkane dehalogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Dijkstra, B W~~]]	+	[[Category: Xanthobacter autotrophicus]]
-	[[Category: ~~Kalk, K H~~]]	+	[[Category: Dijkstra BW]]
-	[[Category: ~~Ridder, I S~~]]	+	[[Category: Kalk KH]]
-	[[Category: ~~Rozeboom, H J~~]]	+	[[Category: Ridder IS]]
-	[[Category: ~~Alpha/beta-hydrolase]]~~	+	[[Category: Rozeboom HJ]]
-	~~[[Category: Collision complex]]~~	+
-	~~[[Category: Dehalogenase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:48, 25 August 2021

OCA — Wed, 25 Aug 2021 06:48:15 GMT

←Older revision		Revision as of 06:48, 25 August 2021
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	<StructureSection load='1cij' size='340' side='right'caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cij' size='340' side='right'caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [~~http~~://pdbe.org/1cij PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cij PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cij ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [https://pdbe.org/1cij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [https://www.ebi.ac.uk/pdbsum/1cij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cij ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.	+	[[https://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 15:43, 20 November 2019

OCA — Wed, 20 Nov 2019 15:43:06 GMT

←Older revision		Revision as of 15:43, 20 November 2019
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	==HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE==		==HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE==
-	<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1cij' size='340' side='right'caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Dehalogenase\|Dehalogenase]]	+	*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	[[Category: Corynebacterium autotrophicum baumgarten et al. 1974]]		[[Category: Corynebacterium autotrophicum baumgarten et al. 1974]]
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
		+	[[Category: Large Structures]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Kalk, K H]]		[[Category: Kalk, K H]]

OCA at 05:25, 8 March 2018

OCA — Thu, 08 Mar 2018 05:25:18 GMT

←Older revision		Revision as of 05:25, 8 March 2018
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/1cij_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/1cij_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1cij" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cij" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dehalogenase\|Dehalogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:18, 29 November 2017

OCA — Wed, 29 Nov 2017 08:18:00 GMT

←Older revision		Revision as of 08:18, 29 November 2017
Line 1:		Line 1:
		+
	==HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE==		==HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE==
	<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://pdbe.org/1cij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://pdbe.org/1cij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cij ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1cij" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cij" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dehalogenase\|Dehalogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:46, 8 February 2016

OCA — Mon, 08 Feb 2016 00:46:22 GMT

←Older revision		Revision as of 00:46, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cij ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:19, 10 September 2015

OCA — Thu, 10 Sep 2015 06:19:08 GMT

←Older revision		Revision as of 06:19, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Xanthobacter_autotrophicus Xanthobacter autotrophicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"corynebacterium_autotrophicum"_baumgarten_et_al._1974 "corynebacterium autotrophicum" baumgarten et al. 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://pdbe.org/1cij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cij" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Corynebacterium autotrophicum baumgarten et al. 1974]]
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
-	[[Category: Xanthobacter autotrophicus]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]
	[[Category: Kalk, K H]]		[[Category: Kalk, K H]]

OCA at 19:19, 24 December 2014

OCA — Wed, 24 Dec 2014 19:19:46 GMT

←Older revision		Revision as of 19:19, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DHLA_XANAU DHLA_XANAU]] Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 21:14, 22 December 2014

OCA — Mon, 22 Dec 2014 21:14:51 GMT

←Older revision		Revision as of 21:14, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
	[[Category: Xanthobacter autotrophicus]]		[[Category: Xanthobacter autotrophicus]]
-	[[Category: Dijkstra, B W.]]	+	[[Category: Dijkstra, B W]]
-	[[Category: Kalk, K H.]]	+	[[Category: Kalk, K H]]
-	[[Category: Ridder, I S.]]	+	[[Category: Ridder, I S]]
-	[[Category: Rozeboom, H J.]]	+	[[Category: Rozeboom, H J]]
	[[Category: Alpha/beta-hydrolase]]		[[Category: Alpha/beta-hydrolase]]
	[[Category: Collision complex]]		[[Category: Collision complex]]
	[[Category: Dehalogenase]]		[[Category: Dehalogenase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 17:12, 20 August 2014

OCA — Wed, 20 Aug 2014 17:12:31 GMT

←Older revision		Revision as of 17:12, 20 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cij.~~png\|left~~\|200px]]	+	==HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE==
		+	<StructureSection load='1cij' size='340' side='right' caption='[[1cij]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CIJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cij OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cij RCSB], [http://www.ebi.ac.uk/pdbsum/1cij PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/1cij_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Haloalkane dehalogenase (DhlA) converts haloalkanes to their corresponding alcohols and halide ions. The rate-limiting step in the reaction of DhlA is the release of the halide ion. The kinetics of halide release have been analyzed by measuring halide binding with stopped-flow fluorescence experiments. At high halide concentrations, halide import occurs predominantly via the rapid formation of a weak initial collision complex, followed by transport of the ion to the active site. To obtain more insight in this collision complex, we determined the X-ray structure of DhlA in the presence of bromide and investigated the kinetics of mutants that were constructed on the basis of this structure. The X-ray structure revealed one bromide ion firmly bound in the active site and two bromide ions weakly bound on the surface of the enzyme. One of the weakly bound ions is close to Thr197 and Phe294, near the entrance of the earlier proposed tunnel for substrate import. Kinetic analysis of bromide import by the Thr197Ala and Phe294Ala mutants of DhlA at high halide concentration showed that the rate constants for halide binding no longer displayed a wild-type-like parabolic increase with increasing bromide concentrations. This is in agreement with an elimination or a decrease in affinity of the surface-located halide-binding site. Likewise, chloride binding kinetics of the mutants indicated significant differences with wild-type enzyme. The results indicate that Thr197 and Phe294 are involved in the formation of an initial collision complex for halide import in DhlA and provide experimental evidence for the role of the tunnel in substrate and product transport.

-	~~{{STRUCTURE_1cij\| PDB=1cij \| SCENE= }}~~	+	Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase.,Pikkemaat MG, Ridder IS, Rozeboom HJ, Kalk KH, Dijkstra BW, Janssen DB Biochemistry. 1999 Sep 14;38(37):12052-61. PMID:10508409<ref>PMID:10508409</ref>

-	~~===HALOALKANE DEHALOGENASE SOAKED WITH HIGH CONCENTRATION OF BROMIDE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10508409}}~~	+	==See Also==
-		+	*[[Dehalogenase\|Dehalogenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1cij~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthobacter_autotrophicus Xanthobacter autotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CIJ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010508409<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
	[[Category: Xanthobacter autotrophicus]]		[[Category: Xanthobacter autotrophicus]]