1cr0 - Revision history

OCA at 06:44, 7 February 2024

OCA — Wed, 07 Feb 2024 06:44:55 GMT

←Older revision		Revision as of 06:44, 7 February 2024
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	<StructureSection load='1cr0' size='340' side='right'caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cr0' size='340' side='right'caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bpt7 Bpt7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [https://pdbe.org/1cr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [https://pdbe.org/1cr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~PRIM_BPT7 PRIM_BPT7~~]~~] Synthesizes short RNA primers~~ for DNA replication. ~~Unwinds~~ the DNA at the replication ~~forks~~ and ~~generates single-stranded DNA~~ for both leading and lagging strand synthesis. ~~The primase~~ synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:~~9096333~~</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>	+	[https://www.uniprot.org/uniprot/HELIC_BPT7 HELIC_BPT7] ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination (PubMed:21606333, PubMed:22977246, PubMed:32009150). The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis (PubMed:21606333, PubMed:22977246, PubMed:32009150). ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA (PubMed:30679383, PubMed:17604719). Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange (PubMed:9096333, PubMed:8617248). Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present (PubMed:6454135, PubMed:9139692).[HAMAP-Rule:MF_04154]<ref>PMID:17604719</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref> <ref>PMID:30679383</ref> <ref>PMID:32009150</ref> <ref>PMID:6454135</ref> <ref>PMID:8617248</ref> <ref>PMID:9096333</ref> <ref>PMID:9139692</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cr0 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cr0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Helicases that unwind DNA at the replication fork are ring-shaped oligomeric enzymes that move along one strand of a DNA duplex and catalyze the displacement of the complementary strand in a reaction that is coupled to nucleotide hydrolysis. The helicase domain of the replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that resembles the Escherichia coli RecA protein, an ATP-dependent DNA strand exchange factor. When viewed in projection along the helical axis of the crystals, six protomers of the T7 helicase domain resemble the hexameric rings seen in electron microscopic images of the intact T7 helicase-primase. Nucleotides bind at the interface between pairs of adjacent subunits where an arginine is near the gamma-phosphate of the nucleotide in trans. The bound nucleotide stabilizes the folded conformation of a DNA-binding motif located near the center of the ring. These and other observations suggest how conformational changes are coupled to DNA unwinding activity.
-
-	Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7.,Sawaya MR, Guo S, Tabor S, Richardson CC, Ellenberger T Cell. 1999 Oct 15;99(2):167-77. PMID:10535735<ref>PMID:10535735</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bpt7~~]]	+	[[Category: Escherichia phage T7]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ellenberger, T]]	+	[[Category: Ellenberger T]]
-	[[Category: Guo, S]]	+	[[Category: Guo S]]
-	[[Category: Richardson~~, C C~~]]	+	[[Category: Richardson CC]]
-	[[Category: Sawaya~~, M R~~]]	+	[[Category: Sawaya MR]]
-	[[Category: Tabor, S]]	+	[[Category: Tabor S]]
-	~~[[Category: Reca-type protein fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:39, 14 July 2021

OCA — Wed, 14 Jul 2021 10:39:19 GMT

←Older revision		Revision as of 10:39, 14 July 2021
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	<StructureSection load='1cr0' size='340' side='right'caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cr0' size='340' side='right'caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [~~http~~://pdbe.org/1cr0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cr0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [https://pdbe.org/1cr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>	+	[[https://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[RNA polymerase\|RNA polymerase]]	+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:51, 10 October 2019

OCA — Thu, 10 Oct 2019 06:51:10 GMT

←Older revision		Revision as of 06:51, 10 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7==		==CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7==
-	<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1cr0' size='340' side='right'caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bpt7]]		[[Category: Bpt7]]
		+	[[Category: Large Structures]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]
	[[Category: Guo, S]]		[[Category: Guo, S]]

OCA at 08:30, 6 December 2017

OCA — Wed, 06 Dec 2017 08:30:15 GMT

←Older revision		Revision as of 08:30, 6 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7==		==CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7==
	<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://pdbe.org/1cr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://pdbe.org/1cr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1cr0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1cr0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[RNA polymerase\|RNA polymerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:24, 8 February 2016

OCA — Mon, 08 Feb 2016 02:24:55 GMT

←Older revision		Revision as of 02:24, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cr0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:59, 10 September 2015

OCA — Thu, 10 Sep 2015 06:59:20 GMT

←Older revision		Revision as of 06:59, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_t7 Enterobacteria phage t7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://pdbe.org/1cr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cr0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage t7~~]]	+	[[Category: Bpt7]]
	[[Category: Ellenberger, T]]		[[Category: Ellenberger, T]]
	[[Category: Guo, S]]		[[Category: Guo, S]]

OCA at 19:52, 24 December 2014

OCA — Wed, 24 Dec 2014 19:52:50 GMT

←Older revision		Revision as of 19:52, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRIM_BPT7 PRIM_BPT7]] Synthesizes short RNA primers for DNA replication. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs.<ref>PMID:9096333</ref> <ref>PMID:21606333</ref> <ref>PMID:22977246</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 21:56, 22 December 2014

OCA — Mon, 22 Dec 2014 21:56:55 GMT

←Older revision		Revision as of 21:56, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
-	[[Category: Ellenberger, T.]]	+	[[Category: Ellenberger, T]]
-	[[Category: Guo, S.]]	+	[[Category: Guo, S]]
-	[[Category: Richardson, C C.]]	+	[[Category: Richardson, C C]]
-	[[Category: Sawaya, M R.]]	+	[[Category: Sawaya, M R]]
-	[[Category: Tabor, S.]]	+	[[Category: Tabor, S]]
	[[Category: Reca-type protein fold]]		[[Category: Reca-type protein fold]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:20, 27 August 2014

OCA — Wed, 27 Aug 2014 09:20:34 GMT

←Older revision		Revision as of 09:20, 27 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1cr0.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7==
		+	<StructureSection load='1cr0' size='340' side='right' caption='[[1cr0]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CR0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cr1\|1cr1]], [[1cr2\|1cr2]], [[1cr4\|1cr4]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cr0 RCSB], [http://www.ebi.ac.uk/pdbsum/1cr0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/1cr0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Helicases that unwind DNA at the replication fork are ring-shaped oligomeric enzymes that move along one strand of a DNA duplex and catalyze the displacement of the complementary strand in a reaction that is coupled to nucleotide hydrolysis. The helicase domain of the replicative helicase-primase protein from bacteriophage T7 crystallized as a helical filament that resembles the Escherichia coli RecA protein, an ATP-dependent DNA strand exchange factor. When viewed in projection along the helical axis of the crystals, six protomers of the T7 helicase domain resemble the hexameric rings seen in electron microscopic images of the intact T7 helicase-primase. Nucleotides bind at the interface between pairs of adjacent subunits where an arginine is near the gamma-phosphate of the nucleotide in trans. The bound nucleotide stabilizes the folded conformation of a DNA-binding motif located near the center of the ring. These and other observations suggest how conformational changes are coupled to DNA unwinding activity.

-	~~{{STRUCTURE_1cr0\| PDB=1cr0 \| SCENE= }}~~	+	Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7.,Sawaya MR, Guo S, Tabor S, Richardson CC, Ellenberger T Cell. 1999 Oct 15;99(2):167-77. PMID:10535735<ref>PMID:10535735</ref>

-	~~===CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10535735}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1cr0]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA].~~	+

	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase\|RNA polymerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010535735<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Enterobacteria phage t7]]		[[Category: Enterobacteria phage t7]]
	[[Category: Ellenberger, T.]]		[[Category: Ellenberger, T.]]

OCA at 08:35, 20 October 2012

OCA — Sat, 20 Oct 2012 08:35:22 GMT

←Older revision		Revision as of 08:35, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1cr0]] is a 1 chain structure ~~of [[RNA polymerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA].	+	[[1cr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CR0 OCA].

	==See Also==		==See Also==