1cuu - Revision history

OCA at 15:43, 13 March 2024

OCA — Wed, 13 Mar 2024 15:43:36 GMT

←Older revision		Revision as of 15:43, 13 March 2024
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	<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_vanettenii Fusarium vanettenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.~~1.~~1.3 3.1.1.3] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.69Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [https://pdbe.org/1cuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [https://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [https://pdbe.org/1cuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [https://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CUTI1_FUSSO CUTI1_FUSSO]~~] Catalyzes the hydrolysis of cutin, a ~~polyester~~ that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of ~~the~~ fungal infection.	+	[https://www.uniprot.org/uniprot/CUTI1_FUSVN CUTI1_FUSVN] Catalyzes the hydrolysis of complex carboxylic polyesters found in the cell wall of plants (PubMed:18658138, PubMed:8286366, PubMed:8555209, PubMed:19810726). Degrades cutin, a macromolecule that forms the structure of the plant cuticle (PubMed:18658138, PubMed:8286366, PubMed:8555209, PubMed:19810726). Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of fungal infection (Ref.4).<ref>PMID:18658138</ref> <ref>PMID:19810726</ref> <ref>PMID:8286366</ref> <ref>PMID:8555209</ref> [PROSITE-ProRule:PRU10109]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cuu ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cuu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In characterizing mutants and covalently inhibited complexes of Fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. Taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. Surface loops were identified as the major flexible protein regions, particularly those forming the active-site groove, whereas the elements constituting the protein scaffold were found to retain the same conformation in all the cutinase variants studied. Flexibility turned out to be correlated with thermal motion. With a given crystal packing environment, a high flexibility turned out to be correlated with a low involvement in crystal packing contacts. The high degree of crystal polymorphism, which allowed different conformations with similar energy to be detected, made it possible to identify motions which would have remained unidentified if only a single crystal form had been available. Fairly good agreement was found to exist between the data obtained from the structural comparison and those from a molecular dynamics (MD) simulation carried out on the native enzyme. The crystallographic approach used in this study turned out to be a suitable tool for investigating cutinase dynamics. Because of the availability of a set of closely related proteins in different crystal environments, the intrinsic drawback of a crystallographic approach was bypassed. By combining several static pictures, the dynamics of the protein could be monitored much more realistically than what can be achieved on the basis of static pictures alone.
-
-	Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants.,Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C Proteins. 1996 Dec;26(4):442-58. PMID:8990497<ref>PMID:8990497</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cuu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fusarium vanettenii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Triacylglycerol lipase]]~~	+	[[Category: Cambillau C]]
-	[[Category: Cambillau, C]]	+	[[Category: Longhi S]]
-	[[Category: Longhi, S]]	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine esterase~~]]	+

OCA at 14:56, 3 November 2021

OCA — Wed, 03 Nov 2021 14:56:28 GMT

←Older revision		Revision as of 14:56, 3 November 2021
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	<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure ~~with sequence from [https://en.wikipedia.org/wiki/Fusso Fusso]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [https://pdbe.org/1cuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [https://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [https://pdbe.org/1cuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [https://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fusso]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]

OCA at 10:40, 14 July 2021

OCA — Wed, 14 Jul 2021 10:40:39 GMT

←Older revision		Revision as of 10:40, 14 July 2021
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	<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [~~http~~://pdbe.org/1cuu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [https://pdbe.org/1cuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [https://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.	+	[[https://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:00, 10 October 2019

OCA — Thu, 10 Oct 2019 07:00:07 GMT

←Older revision		Revision as of 07:00, 10 October 2019
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	==CUTINASE, A199C MUTANT==		==CUTINASE, A199C MUTANT==
-	<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>	+	<StructureSection load='1cuu' size='340' side='right'caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Cutinase\|Cutinase]]	+	*[[Cutinase 3D structures\|Cutinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Fusso]]		[[Category: Fusso]]
		+	[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]

OCA at 08:36, 6 December 2017

OCA — Wed, 06 Dec 2017 08:36:54 GMT

←Older revision		Revision as of 08:36, 6 December 2017
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		+
	==CUTINASE, A199C MUTANT==		==CUTINASE, A199C MUTANT==
	<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://pdbe.org/1cuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://pdbe.org/1cuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cuu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cuu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cuu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:20, 9 February 2016

OCA — Tue, 09 Feb 2016 13:20:01 GMT

←Older revision		Revision as of 13:20, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cuu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:37, 10 September 2015

OCA — Thu, 10 Sep 2015 22:37:37 GMT

←Older revision		Revision as of 22:37, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>		<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Nectria_haematococca_mpvi Nectria haematococca mpvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusso Fusso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://pdbe.org/1cuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cuu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Nectria haematococca mpvi~~]]	+	[[Category: Fusso]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]

OCA at 07:03, 25 December 2014

OCA — Thu, 25 Dec 2014 07:03:28 GMT

←Older revision		Revision as of 07:03, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CUTI1_FUSSO CUTI1_FUSSO]] Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:36, 22 December 2014

OCA — Mon, 22 Dec 2014 18:36:32 GMT

←Older revision		Revision as of 18:36, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca_mpvi Nectria haematococca mpvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca_mpvi Nectria haematococca mpvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Nectria haematococca mpvi]]		[[Category: Nectria haematococca mpvi]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
-	[[Category: Cambillau, C.]]	+	[[Category: Cambillau, C]]
-	[[Category: Longhi, S.]]	+	[[Category: Longhi, S]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine esterase]]		[[Category: Serine esterase]]

OCA at 09:27, 27 August 2014

OCA — Wed, 27 Aug 2014 09:27:00 GMT

←Older revision		Revision as of 09:27, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1cuu.~~png\|left~~\|200px]]	+	==CUTINASE, A199C MUTANT==
		+	<StructureSection load='1cuu' size='340' side='right' caption='[[1cuu]], [[Resolution\|resolution]] 1.69Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cuu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca_mpvi Nectria haematococca mpvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CUU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cuu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cuu RCSB], [http://www.ebi.ac.uk/pdbsum/1cuu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/1cuu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In characterizing mutants and covalently inhibited complexes of Fusarium solani cutinase, which is a 197-residue lipolytic enzyme, 34 variant structures, crystallizing in 8 different crystal forms, have been determined, mostly at high resolution. Taking advantage of this considerable body of information, a structural comparative analysis was carried out to investigate the dynamics of cutinase. Surface loops were identified as the major flexible protein regions, particularly those forming the active-site groove, whereas the elements constituting the protein scaffold were found to retain the same conformation in all the cutinase variants studied. Flexibility turned out to be correlated with thermal motion. With a given crystal packing environment, a high flexibility turned out to be correlated with a low involvement in crystal packing contacts. The high degree of crystal polymorphism, which allowed different conformations with similar energy to be detected, made it possible to identify motions which would have remained unidentified if only a single crystal form had been available. Fairly good agreement was found to exist between the data obtained from the structural comparison and those from a molecular dynamics (MD) simulation carried out on the native enzyme. The crystallographic approach used in this study turned out to be a suitable tool for investigating cutinase dynamics. Because of the availability of a set of closely related proteins in different crystal environments, the intrinsic drawback of a crystallographic approach was bypassed. By combining several static pictures, the dynamics of the protein could be monitored much more realistically than what can be achieved on the basis of static pictures alone.

-	~~{{STRUCTURE_1cuu\| PDB=1cuu \| SCENE= }}~~	+	Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants.,Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C Proteins. 1996 Dec;26(4):442-58. PMID:8990497<ref>PMID:8990497</ref>

-	~~===CUTINASE~~, ~~A199C MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_8990497}}~~	+	==See Also==
-		+	*[[Cutinase\|Cutinase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1cuu~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nectria_haematococca_mpvi Nectria haematococca mpvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CUU OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:008990497<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Nectria haematococca mpvi]]		[[Category: Nectria haematococca mpvi]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]