1cyq - Revision history

OCA at 06:46, 7 February 2024

OCA — Wed, 07 Feb 2024 06:46:43 GMT

←Older revision		Revision as of 06:46, 7 February 2024
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	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.		[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.
-
-	A novel endonuclease mechanism directly visualized for I-PpoI.,Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547<ref>PMID:10581547</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1cyq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:10, 9 August 2023

OCA — Wed, 09 Aug 2023 07:10:35 GMT

←Older revision		Revision as of 07:10, 9 August 2023
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	<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Phypo Phypo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1a73\|1a73]]~~, ~~[[1a74\|1a74]], [[1ipp\|1ipp]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [https://pdbe.org/1cyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [https://www.ebi.ac.uk/pdbsum/1cyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [https://pdbe.org/1cyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [https://www.ebi.ac.uk/pdbsum/1cyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO]] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.	+	[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phypo~~]]	+	[[Category: Physarum polycephalum]]
-	[[Category: Chevalier, B]]	+	[[Category: Chevalier B]]
-	[[Category: Flick~~, K E~~]]	+	[[Category: Flick KE]]
-	[[Category: Galburt~~, E A~~]]	+	[[Category: Galburt EA]]
-	[[Category: Jurica~~, M S~~]]	+	[[Category: Jurica MS]]
-	[[Category: Stoddard~~, B L]]~~	+	[[Category: Stoddard BL]]
-	~~[[Category: Beta sheet dna binding]]~~	+
-	~~[[Category: Distorted dna]]~~	+
-	~~[[Category: His-cys box zinc binding site]]~~	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Protein-dna complex~~]]	+

OCA at 14:57, 3 November 2021

OCA — Wed, 03 Nov 2021 14:57:25 GMT

←Older revision		Revision as of 14:57, 3 November 2021
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	<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Phypo Phypo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</div></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Phypo]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]
	[[Category: Flick, K E]]		[[Category: Flick, K E]]

OCA at 15:20, 3 March 2021

OCA — Wed, 03 Mar 2021 15:20:19 GMT

←Older revision		Revision as of 15:20, 3 March 2021
Line 3:		Line 3:
	<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [~~http~~://pdbe.org/1cyq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1cyq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [https://pdbe.org/1cyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [https://www.ebi.ac.uk/pdbsum/1cyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO]] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.	+	[[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO]] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:33, 29 May 2019

OCA — Wed, 29 May 2019 07:33:03 GMT

←Older revision		Revision as of 07:33, 29 May 2019
Line 1:		Line 1:

	==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX==		==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX==
-	<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>	+	<StructureSection load='1cyq' size='340' side='right'caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]
	[[Category: Flick, K E]]		[[Category: Flick, K E]]

OCA at 11:16, 24 August 2017

OCA — Thu, 24 Aug 2017 11:16:35 GMT

←Older revision		Revision as of 11:16, 24 August 2017
Line 1:		Line 1:
		+
	==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX==		==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX==
	<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Phypo Phypo]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://pdbe.org/1cyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://pdbe.org/1cyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1cyq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Phypo]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]
	[[Category: Flick, K E]]		[[Category: Flick, K E]]

OCA at 08:46, 11 September 2015

OCA — Fri, 11 Sep 2015 08:46:56 GMT

←Older revision		Revision as of 08:46, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Physarum_polycephalum Physarum polycephalum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Phypo Phypo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://pdbe.org/1cyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1cyq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Physarum polycephalum~~]]	+	[[Category: Phypo]]
	[[Category: Chevalier, B]]		[[Category: Chevalier, B]]
	[[Category: Flick, K E]]		[[Category: Flick, K E]]

OCA at 14:03, 25 December 2014

OCA — Thu, 25 Dec 2014 14:03:22 GMT

←Older revision		Revision as of 14:03, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO]] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 23:11, 22 December 2014

OCA — Mon, 22 Dec 2014 23:11:49 GMT

←Older revision		Revision as of 23:11, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Physarum polycephalum]]		[[Category: Physarum polycephalum]]
-	[[Category: Chevalier, B.]]	+	[[Category: Chevalier, B]]
-	[[Category: Flick, K E.]]	+	[[Category: Flick, K E]]
-	[[Category: Galburt, E A.]]	+	[[Category: Galburt, E A]]
-	[[Category: Jurica, M S.]]	+	[[Category: Jurica, M S]]
-	[[Category: Stoddard, B L.]]	+	[[Category: Stoddard, B L]]
	[[Category: Beta sheet dna binding]]		[[Category: Beta sheet dna binding]]
	[[Category: Distorted dna]]		[[Category: Distorted dna]]

OCA at 09:19, 27 August 2014

OCA — Wed, 27 Aug 2014 09:19:50 GMT

←Older revision		Revision as of 09:19, 27 August 2014
Line 1:		Line 1:
-	[[~~Image:~~1cyq.~~png~~\|~~left~~\|~~200px~~]]	+	==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)/DNA HOMING SITE COMPLEX==
		+	<StructureSection load='1cyq' size='340' side='right' caption='[[1cyq]], [[Resolution\|resolution]] 1.93Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1cyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1CYQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a73\|1a73]], [[1a74\|1a74]], [[1ipp\|1ipp]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1cyq RCSB], [http://www.ebi.ac.uk/pdbsum/1cyq PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.

-	~~{{STRUCTURE_1cyq\| PDB=1cyq \| SCENE= }}~~	+	A novel endonuclease mechanism directly visualized for I-PpoI.,Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547<ref>PMID:10581547</ref>

-	~~===INTRON ENCODED HOMING ENDONUCLEASE I-PPOI (H98A)~~/~~DNA HOMING SITE COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10581547}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1cyq]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Physarum_polycephalum Physarum polycephalum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1CYQ OCA].~~	+

	==See Also==		==See Also==
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010581547<~~/~~ref~~><~~ref group="xtra">PMID:015048824</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Physarum polycephalum]]		[[Category: Physarum polycephalum]]
	[[Category: Chevalier, B.]]		[[Category: Chevalier, B.]]