1dbn - Revision history

OCA at 09:49, 20 March 2024

OCA — Wed, 20 Mar 2024 09:49:11 GMT

←Older revision		Revision as of 09:49, 20 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.75Å</td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [https://pdbe.org/1dbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [https://www.ebi.ac.uk/pdbsum/1dbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [https://pdbe.org/1dbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [https://www.ebi.ac.uk/pdbsum/1dbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALB_MAAAM MALB_MAAAM]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Seeds from the legume tree Maackia amurensis contain two lectins that can agglutinate different blood cell types. Their specificity toward sialylated oligosaccharides is unique among legume lectins; the leukoagglutinin preferentially binds to sialyllactosamine (alphaNeuAc(2-3)betaGal(1-4)betaGlcNAc), whereas the hemagglutinin displays higher affinity for a disialylated tetrasaccharide (alphaNeuAc(2-3)betaGal(1-3)[alphaNeuAc(2-6)]alphaG alNAc). The three-dimensional structure of the complex between M. amurensis leukoagglutinin and sialyllactose has been determined at 2.75-A resolution using x-ray crystallography. The carbohydrate binding site consists of a deep cleft that accommodates the three carbohydrate residues of the sialyllactose. The central galactose sits in the primary binding site in an orientation that has not been observed previously in other legume lectins. The carboxyl group of sialic acid establishes a salt bridge with a lysine side chain. The glucose residue is very efficiently docked between two tyrosine aromatic rings. The complex between M. amurensis hemagglutinin and a disialylated tetrasaccharide could be modeled from the leukoagglutinin/sialyllactose crystal structure. The substitution of one tyrosine by an alanine residue is responsible for the difference in fine specificity between the two isolectins. Comparison with other legume lectins indicates that oligosaccharide specificity within this family is achieved by the recycling of structural loops in different combinations.
-
-	An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides.,Imberty A, Gautier C, Lescar J, Perez S, Wyns L, Loris R J Biol Chem. 2000 Jun 9;275(23):17541-8. PMID:10747930<ref>PMID:10747930</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1dbn" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Maackia amurensis]]		[[Category: Maackia amurensis]]
-	[[Category: Gautier, C]]	+	[[Category: Gautier C]]
-	[[Category: Imberty, A]]	+	[[Category: Imberty A]]
-	[[Category: Lescar, J]]	+	[[Category: Lescar J]]
-	[[Category: Loris, R]]	+	[[Category: Loris R]]
-	~~[[Category: Carbohydrate binding]]~~	+
-	~~[[Category: Plant lectin]]~~	+
-	~~[[Category: Sialyllactose]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 18:24, 10 March 2021

OCA — Wed, 10 Mar 2021 18:24:32 GMT

←Older revision		Revision as of 18:24, 10 March 2021
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	<StructureSection load='1dbn' size='340' side='right'caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='1dbn' size='340' side='right'caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DBN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA~~</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL~~</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [~~http~~://pdbe.org/1dbn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1dbn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbn ProSAT]</span></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [https://pdbe.org/1dbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [https://www.ebi.ac.uk/pdbsum/1dbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbn ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 23:36, 5 June 2019

OCA — Wed, 05 Jun 2019 23:36:57 GMT

←Older revision		Revision as of 23:36, 5 June 2019
Line 1:		Line 1:

	==MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE==		==MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE==
-	<StructureSection load='1dbn' size='340' side='right' caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>	+	<StructureSection load='1dbn' size='340' side='right'caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Maackia amurensis]]		[[Category: Maackia amurensis]]
	[[Category: Gautier, C]]		[[Category: Gautier, C]]

OCA at 04:09, 30 August 2017

OCA — Wed, 30 Aug 2017 04:09:20 GMT

←Older revision		Revision as of 04:09, 30 August 2017
Line 1:		Line 1:
		+
	==MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE==		==MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE==
	<StructureSection load='1dbn' size='340' side='right' caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>		<StructureSection load='1dbn' size='340' side='right' caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://pdbe.org/1dbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://pdbe.org/1dbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>	+
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:54, 11 September 2015

OCA — Fri, 11 Sep 2015 15:54:37 GMT

←Older revision		Revision as of 15:54, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://pdbe.org/1dbn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1dbn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:15, 23 December 2014

OCA — Tue, 23 Dec 2014 00:15:10 GMT

←Older revision		Revision as of 00:15, 23 December 2014
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Maackia amurensis]]		[[Category: Maackia amurensis]]
-	[[Category: Gautier, C.]]	+	[[Category: Gautier, C]]
-	[[Category: Imberty, A.]]	+	[[Category: Imberty, A]]
-	[[Category: Lescar, J.]]	+	[[Category: Lescar, J]]
-	[[Category: Loris, R.]]	+	[[Category: Loris, R]]
	[[Category: Carbohydrate binding]]		[[Category: Carbohydrate binding]]
	[[Category: Plant lectin]]		[[Category: Plant lectin]]
	[[Category: Sialyllactose]]		[[Category: Sialyllactose]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 06:28, 10 October 2014

OCA — Fri, 10 Oct 2014 06:28:55 GMT

←Older revision		Revision as of 06:28, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:38, 4 September 2014

OCA — Thu, 04 Sep 2014 06:38:58 GMT

←Older revision		Revision as of 06:38, 4 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1dbn.~~png~~\|~~left~~\|200px]]	+	==MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE==
		+	<StructureSection load='1dbn' size='340' side='right' caption='[[1dbn]], [[Resolution\|resolution]] 2.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbn RCSB], [http://www.ebi.ac.uk/pdbsum/1dbn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Seeds from the legume tree Maackia amurensis contain two lectins that can agglutinate different blood cell types. Their specificity toward sialylated oligosaccharides is unique among legume lectins; the leukoagglutinin preferentially binds to sialyllactosamine (alphaNeuAc(2-3)betaGal(1-4)betaGlcNAc), whereas the hemagglutinin displays higher affinity for a disialylated tetrasaccharide (alphaNeuAc(2-3)betaGal(1-3)[alphaNeuAc(2-6)]alphaG alNAc). The three-dimensional structure of the complex between M. amurensis leukoagglutinin and sialyllactose has been determined at 2.75-A resolution using x-ray crystallography. The carbohydrate binding site consists of a deep cleft that accommodates the three carbohydrate residues of the sialyllactose. The central galactose sits in the primary binding site in an orientation that has not been observed previously in other legume lectins. The carboxyl group of sialic acid establishes a salt bridge with a lysine side chain. The glucose residue is very efficiently docked between two tyrosine aromatic rings. The complex between M. amurensis hemagglutinin and a disialylated tetrasaccharide could be modeled from the leukoagglutinin/sialyllactose crystal structure. The substitution of one tyrosine by an alanine residue is responsible for the difference in fine specificity between the two isolectins. Comparison with other legume lectins indicates that oligosaccharide specificity within this family is achieved by the recycling of structural loops in different combinations.

-	~~{{STRUCTURE_1dbn\| PDB=1dbn \| SCENE= }}~~	+	An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides.,Imberty A, Gautier C, Lescar J, Perez S, Wyns L, Loris R J Biol Chem. 2000 Jun 9;275(23):17541-8. PMID:10747930<ref>PMID:10747930</ref>

-	~~===MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10747930}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1dbn]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Maackia_amurensis Maackia amurensis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010747930<~~/~~ref~~><~~ref group="xtra">PMID:011491289</ref><references group="xtra"~~/>	+
	[[Category: Maackia amurensis]]		[[Category: Maackia amurensis]]
	[[Category: Gautier, C.]]		[[Category: Gautier, C.]]

OCA: Protected "1dbn" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 20:11:31 GMT

Protected "1dbn" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:11, 21 October 2012

OCA at 20:11, 21 October 2012

OCA — Sun, 21 Oct 2012 20:11:26 GMT

←Older revision		Revision as of 20:11, 21 October 2012
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-	{{Seed}}
	[[Image:1dbn.png\|left\|200px]]		[[Image:1dbn.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1dbn\| PDB=1dbn \| SCENE= }}		{{STRUCTURE_1dbn\| PDB=1dbn \| SCENE= }}

	===MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE===		===MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10747930}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10747930 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_10747930}}		{{ABSTRACT_PUBMED_10747930}}

	==About this Structure==		==About this Structure==
-	~~1DBN~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA].	+	[[1dbn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10747930~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010747930</ref><ref group="xtra">PMID:011491289</ref><references group="xtra"/>
	[[Category: Maackia amurensis]]		[[Category: Maackia amurensis]]
	[[Category: Gautier, C.]]		[[Category: Gautier, C.]]
Line 33:		Line 21:
	[[Category: Sialyllactose]]		[[Category: Sialyllactose]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 22 14:49:35 2010''