1dbw - Revision history

OCA at 06:51, 7 February 2024

OCA — Wed, 07 Feb 2024 06:51:47 GMT

←Older revision		Revision as of 06:51, 7 February 2024
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	<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_9930 Atcc 9930~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [https://pdbe.org/1dbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [https://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [https://pdbe.org/1dbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [https://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME]] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.	+	[https://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbw ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Two-component signal transduction pathways are sophisticated phosphorelay cascades widespread in prokaryotes and also found in fungi, molds and plants. FixL/FixJ is a prototypical system responsible for the regulation of nitrogen fixation in the symbiotic bacterium Sinorhizobium meliloti. In microaerobic conditions the membrane-bound kinase FixL uses ATP to transphosphorylate a histidine residue, and the response regulator FixJ transfers the phosphoryl group from the phosphohistidine to one of its own aspartate residues in a Mg(2+)-dependent mechanism. RESULTS: Seven X-ray structures of the unphosphorylated N-terminal receiver domain of FixJ (FixJN) have been solved from two crystal forms soaked in different conditions. Three conformations of the protein were found. In the first case, the protein fold impairs metal binding in the active site and the structure reveals a receiver domain that is self-inhibited for catalysis. In the second conformation, the canonical geometry of the active site is attained, and subsequent metal binding to the protein induces minimal conformational changes. The third conformation illustrates a non-catalytic form of the protein where unwinding of the N terminus of helix alpha 1 has occurred. Interconversion of the canonical and self-inhibited conformations requires a large conformational change of the beta 3-alpha 3 loop region. CONCLUSIONS: These unphosphorylated structures of FixJN stress the importance of flexible peptide segments that delineate the active site. Their movements may act as molecular switches that define the functional status of the protein. Such observations are in line with structural and biochemical results obtained on other response regulator proteins and may illustrate general features that account for the specificity of protein-protein interactions.
-
-	Structural transitions in the FixJ receiver domain.,Gouet P, Fabry B, Guillet V, Birck C, Mourey L, Kahn D, Samama JP Structure. 1999 Dec 15;7(12):1517-26. PMID:10647182<ref>PMID:10647182</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1dbw" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 9930]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Birck, C]]	+	[[Category: Sinorhizobium meliloti]]
-	[[Category: Fabry, B]]	+	[[Category: Birck C]]
-	[[Category: Gouet, P]]	+	[[Category: Fabry B]]
-	[[Category: Guillet, V]]	+	[[Category: Gouet P]]
-	[[Category: Kahn, D]]	+	[[Category: Guillet V]]
-	[[Category: Mourey, L]]	+	[[Category: Kahn D]]
-	[[Category: Samama~~, J P]]~~	+	[[Category: Mourey L]]
-	~~[[Category: Doubly wound five-stranded beta/alpha fold]]~~	+	[[Category: Samama JP]]
-	~~[[Category: Nitrogen fixation regulation]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 06:30, 11 August 2021

OCA — Wed, 11 Aug 2021 06:30:12 GMT

←Older revision		Revision as of 06:30, 11 August 2021
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	<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DBW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [~~http~~://pdbe.org/1dbw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [https://pdbe.org/1dbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [https://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME]] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.	+	[[https://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME]] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:38, 6 November 2019

OCA — Wed, 06 Nov 2019 09:38:31 GMT

←Older revision		Revision as of 09:38, 6 November 2019
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	==CRYSTAL STRUCTURE OF FIXJ-N==		==CRYSTAL STRUCTURE OF FIXJ-N==
-	<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1dbw' size='340' side='right'caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 9930]]		[[Category: Atcc 9930]]
		+	[[Category: Large Structures]]
	[[Category: Birck, C]]		[[Category: Birck, C]]
	[[Category: Fabry, B]]		[[Category: Fabry, B]]

OCA at 08:30, 6 December 2017

OCA — Wed, 06 Dec 2017 08:30:53 GMT

←Older revision		Revision as of 08:30, 6 December 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF FIXJ-N==		==CRYSTAL STRUCTURE OF FIXJ-N==
	<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://pdbe.org/1dbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://pdbe.org/1dbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:34, 7 February 2016

OCA — Sun, 07 Feb 2016 05:34:22 GMT

←Older revision		Revision as of 05:34, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:20, 9 September 2015

OCA — Wed, 09 Sep 2015 22:20:45 GMT

←Older revision		Revision as of 22:20, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sinorhizobium_meliloti Sinorhizobium meliloti~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_9930 Atcc 9930]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://pdbe.org/1dbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1dbw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sinorhizobium meliloti~~]]	+	[[Category: Atcc 9930]]
	[[Category: Birck, C]]		[[Category: Birck, C]]
	[[Category: Fabry, B]]		[[Category: Fabry, B]]

OCA at 08:15, 24 December 2014

OCA — Wed, 24 Dec 2014 08:15:07 GMT

←Older revision		Revision as of 08:15, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FIXJ_RHIME FIXJ_RHIME]] FixJ, when activated by FixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for FixN activation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:51, 22 December 2014

OCA — Mon, 22 Dec 2014 19:51:26 GMT

←Older revision		Revision as of 19:51, 22 December 2014
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Sinorhizobium meliloti]]		[[Category: Sinorhizobium meliloti]]
-	[[Category: Birck, C.]]	+	[[Category: Birck, C]]
-	[[Category: Fabry, B.]]	+	[[Category: Fabry, B]]
-	[[Category: Gouet, P.]]	+	[[Category: Gouet, P]]
-	[[Category: Guillet, V.]]	+	[[Category: Guillet, V]]
-	[[Category: Kahn, D.]]	+	[[Category: Kahn, D]]
-	[[Category: Mourey, L.]]	+	[[Category: Mourey, L]]
-	[[Category: Samama, J P.]]	+	[[Category: Samama, J P]]
	[[Category: Doubly wound five-stranded beta/alpha fold]]		[[Category: Doubly wound five-stranded beta/alpha fold]]
	[[Category: Nitrogen fixation regulation]]		[[Category: Nitrogen fixation regulation]]
	[[Category: Transcription]]		[[Category: Transcription]]

OCA at 06:49, 10 October 2014

OCA — Fri, 10 Oct 2014 06:49:53 GMT

←Older revision		Revision as of 06:49, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:42, 4 September 2014

OCA — Thu, 04 Sep 2014 06:42:10 GMT

←Older revision		Revision as of 06:42, 4 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1dbw.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF FIXJ-N==
		+	<StructureSection load='1dbw' size='340' side='right' caption='[[1dbw]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1dbw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DBW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1d5w\|1d5w]], [[1dcm\|1dcm]], [[1dck\|1dck]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dbw RCSB], [http://www.ebi.ac.uk/pdbsum/1dbw PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Two-component signal transduction pathways are sophisticated phosphorelay cascades widespread in prokaryotes and also found in fungi, molds and plants. FixL/FixJ is a prototypical system responsible for the regulation of nitrogen fixation in the symbiotic bacterium Sinorhizobium meliloti. In microaerobic conditions the membrane-bound kinase FixL uses ATP to transphosphorylate a histidine residue, and the response regulator FixJ transfers the phosphoryl group from the phosphohistidine to one of its own aspartate residues in a Mg(2+)-dependent mechanism. RESULTS: Seven X-ray structures of the unphosphorylated N-terminal receiver domain of FixJ (FixJN) have been solved from two crystal forms soaked in different conditions. Three conformations of the protein were found. In the first case, the protein fold impairs metal binding in the active site and the structure reveals a receiver domain that is self-inhibited for catalysis. In the second conformation, the canonical geometry of the active site is attained, and subsequent metal binding to the protein induces minimal conformational changes. The third conformation illustrates a non-catalytic form of the protein where unwinding of the N terminus of helix alpha 1 has occurred. Interconversion of the canonical and self-inhibited conformations requires a large conformational change of the beta 3-alpha 3 loop region. CONCLUSIONS: These unphosphorylated structures of FixJN stress the importance of flexible peptide segments that delineate the active site. Their movements may act as molecular switches that define the functional status of the protein. Such observations are in line with structural and biochemical results obtained on other response regulator proteins and may illustrate general features that account for the specificity of protein-protein interactions.

-	~~{{STRUCTURE_1dbw\| PDB=1dbw \| SCENE= }}~~	+	Structural transitions in the FixJ receiver domain.,Gouet P, Fabry B, Guillet V, Birck C, Mourey L, Kahn D, Samama JP Structure. 1999 Dec 15;7(12):1517-26. PMID:10647182<ref>PMID:10647182</ref>

-	~~===CRYSTAL STRUCTURE OF FIXJ-N===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10647182}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1dbw]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1DBW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010647182<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sinorhizobium meliloti]]		[[Category: Sinorhizobium meliloti]]
	[[Category: Birck, C.]]		[[Category: Birck, C.]]