1dq1 - Revision history

OCA at 06:57, 7 February 2024

OCA — Wed, 07 Feb 2024 06:57:37 GMT

←Older revision		Revision as of 06:57, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [https://pdbe.org/1dq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [https://www.ebi.ac.uk/pdbsum/1dq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dq1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [https://pdbe.org/1dq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [https://www.ebi.ac.uk/pdbsum/1dq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dq1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CONA_CANBR CONA_CANBR]] Glucose/D-mannose specific lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>	+	[https://www.uniprot.org/uniprot/CONA_CANBR CONA_CANBR] Glucose/D-mannose specific lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dq1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dq1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The reversible binding of manganese and calcium to concanavalin A determines the carbohydrate binding of the lectin by inducing large conformational changes. These changes are governed by the isomerization of a non-proline peptide bond, Ala-207-Asp-208, positioned in a beta-strand in between the calcium binding site S2 and the carbohydrate specificity-determining loop. The replacement of calcium by manganese allowed us to investigate the structures of the carbohydrate binding, locked state and the inactive, unlocked state of concanavalin A, both with and without metal ions bound. Crystals of unlocked metal-free concanavalin A convert to the locked form with the binding of two Mn(2+) ions. Removal of these ions from the crystals traps metal-free concanavalin A in its locked state, a minority species in solution. The ligation of a metal ion in S2 to unlocked concanavalin A causes bending of the beta-strand foregoing the S2 ligand residues Asp-10 and Tyr-12. This bending disrupts conventional beta-sheet hydrogen bonding and forces the Thr-11 side chain against the Ala-207-Asp-208 peptide bond. The steric strain exerted by Thr-11 is presumed to drive the trans-to-cis isomerization. Upon isomerization, Asp-208 flips into its carbohydrate binding position, and the conformation of the carbohydrate specificity determining loop changes dramatically.
-
-	The structural features of concanavalin A governing non-proline peptide isomerization.,Bouckaert J, Dewallef Y, Poortmans F, Wyns L, Loris R J Biol Chem. 2000 Jun 30;275(26):19778-87. PMID:10748006<ref>PMID:10748006</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 38:		Line 29:
	[[Category: Canavalia ensiformis]]		[[Category: Canavalia ensiformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bouckaert, J]]	+	[[Category: Bouckaert J]]
-	[[Category: Dewallef, Y]]	+	[[Category: Dewallef Y]]
-	[[Category: Loris, R]]	+	[[Category: Loris R]]
-	[[Category: Poortmans, F]]	+	[[Category: Poortmans F]]
-	[[Category: Wyns, L]]	+	[[Category: Wyns L]]
-	~~[[Category: Beta-sheet]]~~	+
-	~~[[Category: Calcium]]~~	+
-	~~[[Category: Calcium binding site]]~~	+
-	~~[[Category: Ion binding site]]~~	+
-	~~[[Category: Leguminosae lectin]]~~	+
-	~~[[Category: S1]]~~	+
-	~~[[Category: S2]]~~	+
-	~~[[Category: Sugar binding protein]]~~	+
-	~~[[Category: Transition metal~~]]	+

OCA at 09:49, 26 May 2021

OCA — Wed, 26 May 2021 09:49:28 GMT

←Older revision		Revision as of 09:49, 26 May 2021
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	<StructureSection load='1dq1' size='340' side='right'caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1dq1' size='340' side='right'caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [~~http~~://pdbe.org/1dq1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1dq1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1dq1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [https://pdbe.org/1dq1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [https://www.ebi.ac.uk/pdbsum/1dq1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dq1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CONA_CANBR CONA_CANBR]] Glucose/D-mannose specific lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>	+	[[https://www.uniprot.org/uniprot/CONA_CANBR CONA_CANBR]] Glucose/D-mannose specific lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:40, 21 August 2019

OCA — Wed, 21 Aug 2019 07:40:41 GMT

←Older revision		Revision as of 07:40, 21 August 2019
Line 1:		Line 1:

	==Calcium;Calcium concanavalin A==		==Calcium;Calcium concanavalin A==
-	<StructureSection load='1dq1' size='340' side='right' caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='1dq1' size='340' side='right'caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dq/1dq1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dq/1dq1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Concanavalin 3D structures\|Concanavalin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia ensiformis]]		[[Category: Canavalia ensiformis]]
		+	[[Category: Large Structures]]
	[[Category: Bouckaert, J]]		[[Category: Bouckaert, J]]
	[[Category: Dewallef, Y]]		[[Category: Dewallef, Y]]

OCA at 09:10, 16 November 2017

OCA — Thu, 16 Nov 2017 09:10:01 GMT

←Older revision		Revision as of 09:10, 16 November 2017
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Concanavalin A\|Concanavalin A]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:33, 3 August 2017

OCA — Thu, 03 Aug 2017 11:33:37 GMT

←Older revision		Revision as of 11:33, 3 August 2017
Line 1:		Line 1:
		+
	==Calcium;Calcium concanavalin A==		==Calcium;Calcium concanavalin A==
	<StructureSection load='1dq1' size='340' side='right' caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1dq1' size='340' side='right' caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://pdbe.org/1dq1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://pdbe.org/1dq1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dq1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~CONA_CANEN CONA_CANEN~~]] D-mannose specific lectin.	+	[[http://www.uniprot.org/uniprot/CONA_CANBR CONA_CANBR]] Glucose/D-mannose specific lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 20 ug/ml.<ref>PMID:1398779</ref> <ref>PMID:7524287</ref> <ref>PMID:8891754</ref> <ref>PMID:18472821</ref> <ref>PMID:9575151</ref> <ref>PMID:10747944</ref> <ref>PMID:19765980</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 42:		Line 43:
	[[Category: Wyns, L]]		[[Category: Wyns, L]]
	[[Category: Beta-sheet]]		[[Category: Beta-sheet]]
		+	[[Category: Calcium]]
	[[Category: Calcium binding site]]		[[Category: Calcium binding site]]
	[[Category: Ion binding site]]		[[Category: Ion binding site]]

OCA at 05:16, 10 February 2016

OCA — Wed, 10 Feb 2016 05:16:42 GMT

←Older revision		Revision as of 05:16, 10 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dq1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:44, 11 September 2015

OCA — Fri, 11 Sep 2015 05:44:44 GMT

←Older revision		Revision as of 05:44, 11 September 2015
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://pdbe.org/1dq1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1dq1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:04, 25 December 2014

OCA — Thu, 25 Dec 2014 12:04:52 GMT

←Older revision		Revision as of 12:04, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN]] D-mannose specific lectin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:48, 22 December 2014

OCA — Mon, 22 Dec 2014 19:48:37 GMT

←Older revision		Revision as of 19:48, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Canavalia ensiformis]]		[[Category: Canavalia ensiformis]]
-	[[Category: Bouckaert, J.]]	+	[[Category: Bouckaert, J]]
-	[[Category: Dewallef, Y.]]	+	[[Category: Dewallef, Y]]
-	[[Category: Loris, R.]]	+	[[Category: Loris, R]]
-	[[Category: Poortmans, F.]]	+	[[Category: Poortmans, F]]
-	[[Category: Wyns, L.]]	+	[[Category: Wyns, L]]
	[[Category: Beta-sheet]]		[[Category: Beta-sheet]]
	[[Category: Calcium binding site]]		[[Category: Calcium binding site]]

OCA at 10:47, 10 September 2014

OCA — Wed, 10 Sep 2014 10:47:26 GMT

←Older revision		Revision as of 10:47, 10 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1dq1.~~png~~\|~~left~~\|200px]]	+	==Calcium;Calcium concanavalin A==
		+	<StructureSection load='1dq1' size='340' side='right' caption='[[1dq1]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1dq1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DQ1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dq0\|1dq0]], [[1dq2\|1dq2]], [[1dq4\|1dq4]], [[1dq5\|1dq5]], [[1dq6\|1dq6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1dq1 RCSB], [http://www.ebi.ac.uk/pdbsum/1dq1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dq/1dq1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The reversible binding of manganese and calcium to concanavalin A determines the carbohydrate binding of the lectin by inducing large conformational changes. These changes are governed by the isomerization of a non-proline peptide bond, Ala-207-Asp-208, positioned in a beta-strand in between the calcium binding site S2 and the carbohydrate specificity-determining loop. The replacement of calcium by manganese allowed us to investigate the structures of the carbohydrate binding, locked state and the inactive, unlocked state of concanavalin A, both with and without metal ions bound. Crystals of unlocked metal-free concanavalin A convert to the locked form with the binding of two Mn(2+) ions. Removal of these ions from the crystals traps metal-free concanavalin A in its locked state, a minority species in solution. The ligation of a metal ion in S2 to unlocked concanavalin A causes bending of the beta-strand foregoing the S2 ligand residues Asp-10 and Tyr-12. This bending disrupts conventional beta-sheet hydrogen bonding and forces the Thr-11 side chain against the Ala-207-Asp-208 peptide bond. The steric strain exerted by Thr-11 is presumed to drive the trans-to-cis isomerization. Upon isomerization, Asp-208 flips into its carbohydrate binding position, and the conformation of the carbohydrate specificity determining loop changes dramatically.

-	~~{{STRUCTURE_1dq1\| PDB=1dq1 \| SCENE= }}~~	+	The structural features of concanavalin A governing non-proline peptide isomerization.,Bouckaert J, Dewallef Y, Poortmans F, Wyns L, Loris R J Biol Chem. 2000 Jun 30;275(26):19778-87. PMID:10748006<ref>PMID:10748006</ref>

-	~~===Calcium~~;~~Calcium concanavalin A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10748006}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1dq1]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Canavalia_ensiformis Canavalia ensiformis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1DQ1 OCA].~~	+

	==See Also==		==See Also==
	*[[Concanavalin A\|Concanavalin A]]		*[[Concanavalin A\|Concanavalin A]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010748006<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Canavalia ensiformis]]		[[Category: Canavalia ensiformis]]
	[[Category: Bouckaert, J.]]		[[Category: Bouckaert, J.]]