1e3a - Revision history

OCA at 11:50, 13 December 2023

OCA — Wed, 13 Dec 2023 11:50:57 GMT

←Older revision		Revision as of 11:50, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1e3a' size='340' side='right'caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1e3a' size='340' side='right'caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3A FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PAC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity~~'><~~td class="sblockLbl"~~><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase]~~, ~~with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [https://pdbe.org/1e3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [https://www.ebi.ac.uk/pdbsum/1e3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [https://pdbe.org/1e3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [https://www.ebi.ac.uk/pdbsum/1e3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PAC_ECOLX PAC_ECOLX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Penicillin amidase]]~~	+	[[Category: Dodson GG]]
-	[[Category: Dodson~~, G G~~]]	+	[[Category: Hewitt L]]
-	[[Category: Hewitt, L]]	+	[[Category: Kasche V]]
-	[[Category: Kasche, V]]	+	[[Category: Lewis RJ]]
-	[[Category: Lewis~~, R J~~]]	+	[[Category: Lummer K]]
-	[[Category: Lummer, K]]	+	[[Category: Murshudov GN]]
-	[[Category: Murshudov~~, G N~~]]	+	[[Category: Verma CS]]
-	[[Category: Verma~~, C S~~]]	+	[[Category: Wilson KS]]
-	[[Category: Wilson~~, K S]]~~	+
-	~~[[Category: Amidase]]~~	+
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Hydrolysis of penicillin g acylase]]~~	+
-	~~[[Category: Ntn-hydrolase~~]]	+

OCA at 05:45, 28 April 2021

OCA — Wed, 28 Apr 2021 05:45:18 GMT

←Older revision		Revision as of 05:45, 28 April 2021
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	<StructureSection load='1e3a' size='340' side='right'caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1e3a' size='340' side='right'caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1E3A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [~~http~~://pdbe.org/1e3a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1e3a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [https://pdbe.org/1e3a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [https://www.ebi.ac.uk/pdbsum/1e3a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:35, 24 July 2019

OCA — Wed, 24 Jul 2019 06:35:24 GMT

←Older revision		Revision as of 06:35, 24 July 2019
Line 1:		Line 1:

	==A slow processing precursor penicillin acylase from Escherichia coli==		==A slow processing precursor penicillin acylase from Escherichia coli==
-	<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1e3a' size='340' side='right'caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E3A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E3A FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1e3a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1e3a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Penicillin acylase\|Penicillin acylase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
	[[Category: Dodson, G G]]		[[Category: Dodson, G G]]

OCA at 07:52, 13 December 2017

OCA — Wed, 13 Dec 2017 07:52:26 GMT

←Older revision		Revision as of 07:52, 13 December 2017
Line 1:		Line 1:
		+
	==A slow processing precursor penicillin acylase from Escherichia coli==		==A slow processing precursor penicillin acylase from Escherichia coli==
	<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://pdbe.org/1e3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://pdbe.org/1e3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/1e3a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/1e3a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1e3a" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1e3a" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Penicillin acylase\|Penicillin acylase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:36, 9 February 2016

OCA — Tue, 09 Feb 2016 00:36:16 GMT

←Older revision		Revision as of 00:36, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e3a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:53, 10 September 2015

OCA — Thu, 10 Sep 2015 16:53:54 GMT

←Older revision		Revision as of 16:53, 10 September 2015
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://pdbe.org/1e3a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1e3a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 19:17, 22 December 2014

OCA — Mon, 22 Dec 2014 19:17:13 GMT

←Older revision		Revision as of 19:17, 22 December 2014
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	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
-	[[Category: Dodson, G G.]]	+	[[Category: Dodson, G G]]
-	[[Category: Hewitt, L.]]	+	[[Category: Hewitt, L]]
-	[[Category: Kasche, V.]]	+	[[Category: Kasche, V]]
-	[[Category: Lewis, R J.]]	+	[[Category: Lewis, R J]]
-	[[Category: Lummer, K.]]	+	[[Category: Lummer, K]]
-	[[Category: Murshudov, G N.]]	+	[[Category: Murshudov, G N]]
-	[[Category: Verma, C S.]]	+	[[Category: Verma, C S]]
-	[[Category: Wilson, K S.]]	+	[[Category: Wilson, K S]]
	[[Category: Amidase]]		[[Category: Amidase]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Hydrolysis of penicillin g acylase]]		[[Category: Hydrolysis of penicillin g acylase]]
	[[Category: Ntn-hydrolase]]		[[Category: Ntn-hydrolase]]

OCA at 08:43, 22 October 2014

OCA — Wed, 22 Oct 2014 08:43:27 GMT

←Older revision		Revision as of 08:43, 22 October 2014
Line 1:		Line 1:
-	==A ~~SLOW PROCESSING PRECURSOR PENICILLIN ACYLASE FROM ESCHERICHIA COLI~~==	+	==A slow processing precursor penicillin acylase from Escherichia coli==
	<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E3A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E3A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]
	[[Category: Dodson, G G.]]		[[Category: Dodson, G G.]]

OCA at 10:42, 10 September 2014

OCA — Wed, 10 Sep 2014 10:42:42 GMT

←Older revision		Revision as of 10:42, 10 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1e3a.~~png~~\|~~left~~\|200px]]	+	==A SLOW PROCESSING PRECURSOR PENICILLIN ACYLASE FROM ESCHERICHIA COLI==
		+	<StructureSection load='1e3a' size='340' side='right' caption='[[1e3a]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1e3a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E3A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pnk\|1pnk]], [[1pnl\|1pnl]], [[1pnm\|1pnm]], [[1ai4\|1ai4]], [[1ai5\|1ai5]], [[1ai6\|1ai6]], [[1ai7\|1ai7]], [[1ajn\|1ajn]], [[1ajp\|1ajp]], [[1ajq\|1ajq]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PAC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1e3a RCSB], [http://www.ebi.ac.uk/pdbsum/1e3a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/1e3a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Penicillin G acylase is a periplasmic protein, cytoplasmically expressed as a precursor polypeptide comprising a signal sequence, the A and B chains of the mature enzyme (209 and 557 residues respectively) joined by a spacer peptide of 54 amino acid residues. The wild-type AB heterodimer is produced by proteolytic removal of this spacer in the periplasm. The first step in processing is believed to be autocatalytic hydrolysis of the peptide bond between the C-terminal residue of the spacer and the active-site serine residue at the N terminus of the B chain. We have determined the crystal structure of a slowly processing precursor mutant (Thr263Gly) of penicillin G acylase from Escherichia coli, which reveals that the spacer peptide blocks the entrance to the active-site cleft consistent with an autocatalytic mechanism of maturation. In this mutant precursor there is, however, an unexpected cleavage at a site four residues from the active-site serine residue. Analyses of the stereochemistry of the 260-261 bond seen to be cleaved in this precursor structure and of the 263-264 peptide bond have suggested factors that may govern the autocatalytic mechanism.

-	~~{{STRUCTURE_1e3a\| PDB=1e3a \| SCENE= }}~~	+	Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft.,Hewitt L, Kasche V, Lummer K, Lewis RJ, Murshudov GN, Verma CS, Dodson GG, Wilson KS J Mol Biol. 2000 Sep 29;302(4):887-98. PMID:10993730<ref>PMID:10993730</ref>

-	~~===A SLOW PROCESSING PRECURSOR PENICILLIN ACYLASE FROM ESCHERICHIA COLI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10993730}}~~	+	==See Also==
-		+	*[[Penicillin acylase\|Penicillin acylase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1e3a~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3A OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010993730<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Penicillin amidase]]		[[Category: Penicillin amidase]]

OCA: Protected "1e3a" [edit=sysop:move=sysop]

OCA — Wed, 31 Oct 2012 09:52:47 GMT

Protected "1e3a" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:52, 31 October 2012