1esg - Revision history

OCA at 07:06, 7 February 2024

OCA — Wed, 07 Feb 2024 07:06:30 GMT

←Older revision		Revision as of 07:06, 7 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.		[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.
-
-	Structure of BamHI bound to nonspecific DNA: a model for DNA sliding.,Viadiu H, Aggarwal AK Mol Cell. 2000 May;5(5):889-95. PMID:10882125<ref>PMID:10882125</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[BamHI\|BamHI]]		*[[BamHI\|BamHI]]
	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]		*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:21, 18 May 2023

OCA — Thu, 18 May 2023 07:21:32 GMT

←Older revision		Revision as of 07:21, 18 May 2023
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	<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</div></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM]] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.	+	[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Type II site-specific deoxyribonuclease]]~~	+	[[Category: Aggarwal AK]]
-	[[Category: Aggarwal~~, A K~~]]	+	[[Category: Viadiu H]]
-	[[Category: Viadiu, H]]	+
-	~~[[Category: Hydrolase-dna complex]]~~	+
-	~~[[Category: Non-specific dna-protein complex~~]]	+

OCA at 06:32, 11 August 2021

OCA — Wed, 11 Aug 2021 06:32:37 GMT

←Older revision		Revision as of 06:32, 11 August 2021
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	<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ESG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [~~http~~://pdbe.org/1esg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1esg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [https://pdbe.org/1esg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [https://www.ebi.ac.uk/pdbsum/1esg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM]] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.	+	[[https://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM]] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:15, 6 November 2019

OCA — Wed, 06 Nov 2019 09:15:24 GMT

←Older revision		Revision as of 09:15, 6 November 2019
Line 1:		Line 1:

	==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==		==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==
-	<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1esg' size='340' side='right'caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[BamHI\|BamHI]]
		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
	[[Category: Aggarwal, A K]]		[[Category: Aggarwal, A K]]

OCA at 06:14, 31 January 2018

OCA — Wed, 31 Jan 2018 06:14:53 GMT

←Older revision		Revision as of 06:14, 31 January 2018
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	<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
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	</div>		</div>
	<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[BamHI\|BamHI]]
-	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:58, 6 September 2017

OCA — Wed, 06 Sep 2017 03:58:06 GMT

←Older revision		Revision as of 03:58, 6 September 2017
Line 1:		Line 1:
		+
	==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==		==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==
	<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://pdbe.org/1esg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://pdbe.org/1esg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1esg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:03, 10 September 2015

OCA — Thu, 10 Sep 2015 17:03:07 GMT

←Older revision		Revision as of 17:03, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://pdbe.org/1esg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1esg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
	[[Category: Aggarwal, A K]]		[[Category: Aggarwal, A K]]

OCA at 03:20, 25 December 2014

OCA — Thu, 25 Dec 2014 03:20:33 GMT

←Older revision		Revision as of 03:20, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/T2BA_BACAM T2BA_BACAM]] Recognizes the double-stranded sequence GGATCC and cleaves after G-1.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:26, 22 December 2014

OCA — Mon, 22 Dec 2014 19:26:44 GMT

←Older revision		Revision as of 19:26, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]
-	[[Category: Aggarwal, A K.]]	+	[[Category: Aggarwal, A K]]
-	[[Category: Viadiu, H.]]	+	[[Category: Viadiu, H]]
	[[Category: Hydrolase-dna complex]]		[[Category: Hydrolase-dna complex]]
-	[[Category: Non-specific dna-protein complex.]]	+	[[Category: Non-specific dna-protein complex]]

OCA at 11:26, 24 September 2014

OCA — Wed, 24 Sep 2014 11:26:30 GMT

←Older revision		Revision as of 11:26, 24 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1esg.~~png~~\|~~left~~\|~~200px~~]]	+	==RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.==
		+	<StructureSection load='1esg' size='340' side='right' caption='[[1esg]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1esg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ESG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bam\|1bam]], [[2bam\|2bam]], [[3bam\|3bam]], [[1bhm\|1bhm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1esg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1esg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1esg RCSB], [http://www.ebi.ac.uk/pdbsum/1esg PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.

-	~~{{STRUCTURE_1esg\| PDB=1esg \| SCENE= }}~~	+	Structure of BamHI bound to nonspecific DNA: a model for DNA sliding.,Viadiu H, Aggarwal AK Mol Cell. 2000 May;5(5):889-95. PMID:10882125<ref>PMID:10882125</ref>

-	~~===RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10882125}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1esg]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ESG OCA].~~	+

	==See Also==		==See Also==
	*[[BamHI\|BamHI]]		*[[BamHI\|BamHI]]
	*[[Endonuclease\|Endonuclease]]		*[[Endonuclease\|Endonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010882125<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Type II site-specific deoxyribonuclease]]		[[Category: Type II site-specific deoxyribonuclease]]