1etf - Revision history

OCA at 11:48, 22 November 2023

OCA — Wed, 22 Nov 2023 11:48:37 GMT

←Older revision		Revision as of 11:48, 22 November 2023
Line 1:		Line 1:

	==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==		==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='1etf' size='340' side='right'caption='[[1etf~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1etf' size='340' side='right'caption='[[1etf]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1etg\|1etg]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [https://pdbe.org/1etf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [https://www.ebi.ac.uk/pdbsum/1etf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [https://pdbe.org/1etf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [https://www.ebi.ac.uk/pdbsum/1etf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2]] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).	+	[https://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human immunodeficiency virus 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Battiste~~, J L~~]]	+	[[Category: Battiste JL]]
-	[[Category: Frankel~~, A D~~]]	+	[[Category: Frankel AD]]
-	[[Category: Kay~~, L E~~]]	+	[[Category: Kay LE]]
-	[[Category: Mao, H]]	+	[[Category: Mao H]]
-	[[Category: Muhandiram~~, D R~~]]	+	[[Category: Muhandiram DR]]
-	[[Category: Rao~~, N S~~]]	+	[[Category: Rao NS]]
-	[[Category: Tan, R]]	+	[[Category: Tan R]]
-	[[Category: Willamson~~, J R]]~~	+	[[Category: Willamson JR]]
-	~~[[Category: Export regulator]]~~	+
-	~~[[Category: Mrna splicing]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Viral protein-rna complex~~]]	+

OCA at 10:42, 16 February 2022

OCA — Wed, 16 Feb 2022 10:42:06 GMT

←Older revision		Revision as of 10:42, 16 February 2022
Line 3:		Line 3:
	<StructureSection load='1etf' size='340' side='right'caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1etf' size='340' side='right'caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1etg\|1etg]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1etg\|1etg]]</div></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [https://pdbe.org/1etf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [https://www.ebi.ac.uk/pdbsum/1etf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [https://pdbe.org/1etf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [https://www.ebi.ac.uk/pdbsum/1etf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>

OCA at 07:15, 17 March 2021

OCA — Wed, 17 Mar 2021 07:15:23 GMT

←Older revision		Revision as of 07:15, 17 March 2021
Line 3:		Line 3:
	<StructureSection load='1etf' size='340' side='right'caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1etf' size='340' side='right'caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ETF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1etg\|1etg]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [~~http~~://pdbe.org/1etf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1etf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [https://pdbe.org/1etf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [https://www.ebi.ac.uk/pdbsum/1etf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2]] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).	+	[[https://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2]] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:04, 12 June 2019

OCA — Wed, 12 Jun 2019 07:04:21 GMT

←Older revision		Revision as of 07:04, 12 June 2019
Line 1:		Line 1:

	==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==		==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==
-	<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1etf' size='340' side='right'caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Battiste, J L]]		[[Category: Battiste, J L]]
	[[Category: Frankel, A D]]		[[Category: Frankel, A D]]

OCA at 03:50, 6 September 2017

OCA — Wed, 06 Sep 2017 03:50:04 GMT

←Older revision		Revision as of 03:50, 6 September 2017
Line 1:		Line 1:
		+
	==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==		==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==
	<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]~~. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://pdbe.org/1etf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://pdbe.org/1etf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1etf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 15:08, 10 September 2015

OCA — Thu, 10 Sep 2015 15:08:30 GMT

←Older revision		Revision as of 15:08, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_immunodeficiency_virus_1 Human immunodeficiency virus 1~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://pdbe.org/1etf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1etf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Human immunodeficiency virus 1]]
	[[Category: Battiste, J L]]		[[Category: Battiste, J L]]
	[[Category: Frankel, A D]]		[[Category: Frankel, A D]]

OCA at 01:57, 25 December 2014

OCA — Thu, 25 Dec 2014 01:57:07 GMT

←Older revision		Revision as of 01:57, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/REV_HV1W2 REV_HV1W2]] Escorts unspliced or incompletely spliced viral pre-mRNAs (late transcripts) out of the nucleus of infected cells. These pre-mRNAs carry a recognition sequence called Rev responsive element (RRE) located in the env gene, that is not present in fully spliced viral mRNAs (early transcripts). This function is essential since most viral proteins are translated from unspliced or partially spliced pre-mRNAs which cannot exit the nucleus by the pathway used by fully processed cellular mRNAs. Rev itself is translated from a fully spliced mRNA that readily exits the nucleus. Rev's nuclear localization signal (NLS) binds directly to KPNB1/Importin beta-1 without previous binding to KPNA1/Importin alpha-1. KPNB1 binds to the GDP bound form of RAN (Ran-GDP) and targets Rev to the nucleus. In the nucleus, the conversion from Ran-GDP to Ran-GTP dissociates Rev from KPNB1 and allows Rev's binding to the RRE in viral pre-mRNAs. Rev multimerization on the RRE via cooperative assembly exposes its nuclear export signal (NES) to the surface. Rev can then form a complex with XPO1/CRM1 and Ran-GTP, leading to nuclear export of the complex. Conversion from Ran-GTP to Ran-GDP mediates dissociation of the Rev/RRE/XPO1/RAN complex, so that Rev can return to the nucleus for a subsequent round of export. Beside KPNB1, also seems to interact with TNPO1/Transportin-1, RANBP5/IPO5 and IPO7/RANBP7 for nuclear import. The nucleoporin-like HRB/RIP is an essential cofactor that probably indirectly interacts with Rev to release HIV RNAs from the perinuclear region to the cytoplasm (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 23:48, 22 December 2014

OCA — Mon, 22 Dec 2014 23:48:17 GMT

←Older revision		Revision as of 23:48, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</StructureSection>		</StructureSection>
	[[Category: Human immunodeficiency virus 1]]		[[Category: Human immunodeficiency virus 1]]
-	[[Category: Battiste, J L.]]	+	[[Category: Battiste, J L]]
-	[[Category: Frankel, A D.]]	+	[[Category: Frankel, A D]]
-	[[Category: Kay, L E.]]	+	[[Category: Kay, L E]]
-	[[Category: Mao, H.]]	+	[[Category: Mao, H]]
-	[[Category: Muhandiram, D R.]]	+	[[Category: Muhandiram, D R]]
-	[[Category: Rao, N S.]]	+	[[Category: Rao, N S]]
-	[[Category: Tan, R.]]	+	[[Category: Tan, R]]
-	[[Category: Willamson, J R.]]	+	[[Category: Willamson, J R]]
	[[Category: Export regulator]]		[[Category: Export regulator]]
	[[Category: Mrna splicing]]		[[Category: Mrna splicing]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
	[[Category: Viral protein-rna complex]]		[[Category: Viral protein-rna complex]]

OCA at 11:18, 24 September 2014

OCA — Wed, 24 Sep 2014 11:18:03 GMT

←Older revision		Revision as of 11:18, 24 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1etf.~~png~~\|~~left~~\|~~200px~~]]	+	==REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE, NMR, MINIMIZED AVERAGE STRUCTURE==
		+	<StructureSection load='1etf' size='340' side='right' caption='[[1etf]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1etf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ETF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1etg\|1etg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1etf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1etf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1etf RCSB], [http://www.ebi.ac.uk/pdbsum/1etf PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of a human immunodeficiency virus type-1 (HIV-1) Rev peptide bound to stem-loop IIB of the Rev response element (RRE) RNA was solved by nuclear magnetic resonance spectroscopy. The Rev peptide has an alpha-helical conformation and binds in the major groove of the RNA near a purine-rich internal loop. Several arginine side chains make base-specific contacts, and an asparagine residue contacts a G.A base pair. The phosphate backbone adjacent to a G.G base pair adopts an unusual structure that allows the peptide to access a widened major groove. The structure formed by the two purine-purine base pairs of the RRE creates a distinctive binding pocket that the peptide can use for specific recognition.

-	~~{{STRUCTURE_1etf\| PDB=1etf \| SCENE= }}~~	+	Alpha helix-RNA major groove recognition in an HIV-1 rev peptide-RRE RNA complex.,Battiste JL, Mao H, Rao NS, Tan R, Muhandiram DR, Kay LE, Frankel AD, Williamson JR Science. 1996 Sep 13;273(5281):1547-51. PMID:8703216<ref>PMID:8703216</ref>

-	~~===REV RESPONSE ELEMENT (RRE) RNA COMPLEXED WITH REV PEPTIDE~~, ~~NMR, MINIMIZED AVERAGE STRUCTURE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~==About this Structure==~~	+	<references/>
-	~~[[1etf]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1ETF OCA].~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008703216<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Human immunodeficiency virus 1]]		[[Category: Human immunodeficiency virus 1]]
	[[Category: Battiste, J L.]]		[[Category: Battiste, J L.]]

OCA: Protected "1etf" [edit=sysop:move=sysop]

OCA — Wed, 14 Nov 2012 07:29:34 GMT

Protected "1etf" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:29, 14 November 2012