1eww - Revision history

OCA at 10:07, 20 March 2024

OCA — Wed, 20 Mar 2024 10:07:24 GMT

←Older revision		Revision as of 10:07, 20 March 2024
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==		==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==
-	<StructureSection load='1eww' size='340' side='right'caption='[[1eww~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1eww' size='340' side='right'caption='[[1eww]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Archips_fumiferana Archips~~ fumiferana]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [https://pdbe.org/1eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [https://www.ebi.ac.uk/pdbsum/1eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [https://pdbe.org/1eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [https://www.ebi.ac.uk/pdbsum/1eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9GTP0_CHOFU Q9GTP0_CHOFU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eww ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eww ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Insect antifreeze proteins (AFP) are considerably more active at inhibiting ice crystal growth than AFP from fish or plants. Several insect AFPs, also known as thermal hysteresis proteins, have been cloned and expressed. Their maximum activity is 3-4 times that of fish AFPs and they are 10-100 times more effective at micromolar concentrations. Here we report the solution structure of spruce budworm (Choristoneura fumiferana) AFP and characterize its ice-binding properties. The 9-kDa AFP is a beta-helix with a triangular cross-section and rectangular sides that form stacked parallel beta-sheets; a fold which is distinct from the three known fish AFP structures. The ice-binding side contains 9 of the 14 surface-accessible threonines organized in a regular array of TXT motifs that match the ice lattice on both prism and basal planes. In support of this model, ice crystal morphology and ice-etching experiments are consistent with AFP binding to both of these planes and thus may explain the greater activity of the spruce budworm antifreeze.
-
-	Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect.,Graether SP, Kuiper MJ, Gagne SM, Walker VK, Jia Z, Sykes BD, Davies PL Nature. 2000 Jul 20;406(6793):325-8. PMID:10917537<ref>PMID:10917537</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]		*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Antifreeze Proteins]]		[[Category: Antifreeze Proteins]]
-	[[Category: ~~Archips~~ fumiferana]]	+	[[Category: Choristoneura fumiferana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Davies~~, P L~~]]	+	[[Category: Davies PL]]
-	[[Category: Gagne~~, S M~~]]	+	[[Category: Gagne SM]]
-	[[Category: Graether~~, S P~~]]	+	[[Category: Graether SP]]
-	[[Category: Jia, Z]]	+	[[Category: Jia Z]]
-	[[Category: Kuiper~~, M J~~]]	+	[[Category: Kuiper MJ]]
-	[[Category: Sykes~~, B D~~]]	+	[[Category: Sykes BD]]
-	[[Category: Walker~~, V K]]~~	+	[[Category: Walker VK]]
-	~~[[Category: Antifreeze protein]]~~	+
-	~~[[Category: Beta-helix]]~~	+
-	~~[[Category: Ice]]~~	+
-	~~[[Category: Insect~~]]	+

OCA at 11:08, 28 July 2021

OCA — Wed, 28 Jul 2021 11:08:36 GMT

←Older revision		Revision as of 11:08, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1eww' size='340' side='right'caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1eww' size='340' side='right'caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [~~http~~://pdbe.org/1eww PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1eww PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [https://pdbe.org/1eww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [https://www.ebi.ac.uk/pdbsum/1eww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:54, 23 October 2019

OCA — Wed, 23 Oct 2019 08:54:15 GMT

←Older revision		Revision as of 08:54, 23 October 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==		==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==
-	<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1eww' size='340' side='right'caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
Line 25:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antifreeze protein 3D structures\|Antifreeze protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	[[Category: Antifreeze Proteins]]		[[Category: Antifreeze Proteins]]
	[[Category: Archips fumiferana]]		[[Category: Archips fumiferana]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 07:05, 20 December 2017

OCA — Wed, 20 Dec 2017 07:05:23 GMT

←Older revision		Revision as of 07:05, 20 December 2017
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==		==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==
	<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://pdbe.org/1eww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://pdbe.org/1eww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1eww ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 9:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1eww_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1eww_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 24:		Line 25:
	</div>		</div>
	<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Antifreeze protein\|Antifreeze protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:31, 10 February 2016

OCA — Wed, 10 Feb 2016 06:31:17 GMT

←Older revision		Revision as of 06:31, 10 February 2016
Line 13:		Line 13:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eww ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:17, 11 September 2015

OCA — Fri, 11 Sep 2015 06:17:43 GMT

←Older revision		Revision as of 06:17, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Choristoneura_fumiferana Choristoneura~~ fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Archips_fumiferana Archips fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://pdbe.org/1eww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 23:		Line 23:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1eww" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Antifreeze Proteins]]		[[Category: Antifreeze Proteins]]
-	[[Category: ~~Choristoneura~~ fumiferana]]	+	[[Category: Archips fumiferana]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Davies, P L]]		[[Category: Davies, P L]]

OCA at 20:30, 22 December 2014

OCA — Mon, 22 Dec 2014 20:30:43 GMT

←Older revision		Revision as of 20:30, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Choristoneura fumiferana]]		[[Category: Choristoneura fumiferana]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Davies, P L.]]	+	[[Category: Davies, P L]]
-	[[Category: Gagne, S M.]]	+	[[Category: Gagne, S M]]
-	[[Category: Graether, S P.]]	+	[[Category: Graether, S P]]
-	[[Category: Jia, Z.]]	+	[[Category: Jia, Z]]
-	[[Category: Kuiper, M J.]]	+	[[Category: Kuiper, M J]]
-	[[Category: Sykes, B D.]]	+	[[Category: Sykes, B D]]
-	[[Category: Walker, V K.]]	+	[[Category: Walker, V K]]
	[[Category: Antifreeze protein]]		[[Category: Antifreeze protein]]
	[[Category: Beta-helix]]		[[Category: Beta-helix]]
	[[Category: Ice]]		[[Category: Ice]]
	[[Category: Insect]]		[[Category: Insect]]

OCA at 10:22, 28 September 2014

OCA — Sun, 28 Sep 2014 10:22:25 GMT

←Older revision		Revision as of 10:22, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1eww.~~png\|left~~\|200px]]	+	==SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS==
		+	<StructureSection load='1eww' size='340' side='right' caption='[[1eww]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EWW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1eww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eww OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1eww RCSB], [http://www.ebi.ac.uk/pdbsum/1eww PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/1eww_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Insect antifreeze proteins (AFP) are considerably more active at inhibiting ice crystal growth than AFP from fish or plants. Several insect AFPs, also known as thermal hysteresis proteins, have been cloned and expressed. Their maximum activity is 3-4 times that of fish AFPs and they are 10-100 times more effective at micromolar concentrations. Here we report the solution structure of spruce budworm (Choristoneura fumiferana) AFP and characterize its ice-binding properties. The 9-kDa AFP is a beta-helix with a triangular cross-section and rectangular sides that form stacked parallel beta-sheets; a fold which is distinct from the three known fish AFP structures. The ice-binding side contains 9 of the 14 surface-accessible threonines organized in a regular array of TXT motifs that match the ice lattice on both prism and basal planes. In support of this model, ice crystal morphology and ice-etching experiments are consistent with AFP binding to both of these planes and thus may explain the greater activity of the spruce budworm antifreeze.

-	~~{{STRUCTURE_1eww\| PDB=1eww \| SCENE= }}~~	+	Beta-helix structure and ice-binding properties of a hyperactive antifreeze protein from an insect.,Graether SP, Kuiper MJ, Gagne SM, Walker VK, Jia Z, Sykes BD, Davies PL Nature. 2000 Jul 20;406(6793):325-8. PMID:10917537<ref>PMID:10917537</ref>

-	~~===SOLUTION STRUCTURE OF SPRUCE BUDWORM ANTIFREEZE PROTEIN AT 30 DEGREES CELSIUS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10917537}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1eww]] is~~ a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the ~~Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx~~.~~doi~~.~~org/10~~.~~2210~~/~~rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA].~~	+

	==See Also==		==See Also==
	*[[Antifreeze protein\|Antifreeze protein]]		*[[Antifreeze protein\|Antifreeze protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010917537<~~/~~ref~~><~~ref group="xtra">PMID:011880627</ref><ref group="xtra">PMID:012662938</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Antifreeze Proteins]]		[[Category: Antifreeze Proteins]]
	[[Category: Choristoneura fumiferana]]		[[Category: Choristoneura fumiferana]]

OCA at 16:39, 20 October 2012

OCA — Sat, 20 Oct 2012 16:39:49 GMT

←Older revision		Revision as of 16:39, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1eww]] is a 1 chain structure ~~of [[Antifreeze protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA].	+	[[1eww]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. The December 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antifreeze Proteins'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_12 10.2210/rcsb_pdb/mom_2009_12]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EWW OCA].

	==See Also==		==See Also==

OCA: Protected "1eww" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 22:25:33 GMT

Protected "1eww" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:25, 26 July 2012