1ex9 - Revision history

OCA at 10:07, 20 March 2024

OCA — Wed, 20 Mar 2024 10:07:34 GMT

←Older revision		Revision as of 10:07, 20 March 2024
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	<StructureSection load='1ex9' size='340' side='right'caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>		<StructureSection load='1ex9' size='340' side='right'caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL~~-~~PHOSPHINIC+ACID+1~~,2~~-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.54Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.~~1~~.1.3 3.1.1.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [https://pdbe.org/1ex9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [https://pdbe.org/1ex9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LIP_PSEAE LIP_PSEAE]] Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents.	+	[https://www.uniprot.org/uniprot/LIP_PSEAE LIP_PSEAE] Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The x-ray structure of the lipase from Pseudomonas aeruginosa PAO1 has been determined at 2.54 A resolution. It is the first structure of a member of homology family I.1 of bacterial lipases. The structure shows a variant of the alpha/beta hydrolase fold, with Ser(82), Asp(229), and His(251) as the catalytic triad residues. Compared with the "canonical" alpha/beta hydrolase fold, the first two beta-strands and one alpha-helix (alphaE) are not present. The absence of helix alphaE allows the formation of a stabilizing intramolecular disulfide bridge. The loop containing His(251) is stabilized by an octahedrally coordinated calcium ion. On top of the active site a lid subdomain is in an open conformation, making the catalytic cleft accessible from the solvent region. A triacylglycerol analogue is covalently bound to Ser(82) in the active site, demonstrating the position of the oxyanion hole and of the three pockets that accommodate the sn-1, sn-2, and sn-3 fatty acid chains. The inhibited enzyme can be thought to mimic the structure of the tetrahedral intermediate that occurs during the acylation step of the reaction. Analysis of the binding mode of the inhibitor suggests that the size of the acyl pocket and the size and interactions of the sn-2 binding pocket are the predominant determinants of the regio- and enantio-preference of the enzyme.
-
-	Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases.,Nardini M, Lang DA, Liebeton K, Jaeger KE, Dijkstra BW J Biol Chem. 2000 Oct 6;275(40):31219-25. PMID:10893416<ref>PMID:10893416</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ex9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lipase 3D Structures\|Lipase 3D Structures]]		*[[Lipase 3D Structures\|Lipase 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Triacylglycerol lipase~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Dijkstra~~, B W~~]]	+	[[Category: Dijkstra BW]]
-	[[Category: Jaeger, K E]]	+	[[Category: Jaeger K-E]]
-	[[Category: Lang~~, D A~~]]	+	[[Category: Lang DA]]
-	[[Category: Liebeton, K]]	+	[[Category: Liebeton K]]
-	[[Category: Nardini, M]]	+	[[Category: Nardini M]]
-	~~[[Category: Alpha-beta hydrolase fold]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lipase]]~~	+
-	~~[[Category: Phosphonate inhibitor]]~~	+
-	~~[[Category: Pseudomona~~]]	+

OCA at 07:16, 17 March 2021

OCA — Wed, 17 Mar 2021 07:16:39 GMT

←Older revision		Revision as of 07:16, 17 March 2021
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	<StructureSection load='1ex9' size='340' side='right'caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>		<StructureSection load='1ex9' size='340' side='right'caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [~~http~~://pdbe.org/1ex9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ex9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [https://pdbe.org/1ex9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ex9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LIP_PSEAE LIP_PSEAE]] Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents.	+	[[https://www.uniprot.org/uniprot/LIP_PSEAE LIP_PSEAE]] Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Lipase\|Lipase]]	+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:04, 12 June 2019

OCA — Wed, 12 Jun 2019 07:04:30 GMT

←Older revision		Revision as of 07:04, 12 June 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE==		==CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE==
-	<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>	+	<StructureSection load='1ex9' size='340' side='right'caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]

OCA at 03:50, 6 September 2017

OCA — Wed, 06 Sep 2017 03:50:06 GMT

←Older revision		Revision as of 03:50, 6 September 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE==		==CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE==
	<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>		<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://pdbe.org/1ex9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://pdbe.org/1ex9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ex9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ex9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:51, 11 September 2015

OCA — Fri, 11 Sep 2015 10:51:41 GMT

←Older revision		Revision as of 10:51, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>		<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://pdbe.org/1ex9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ex9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]

OCA at 15:27, 25 December 2014

OCA — Thu, 25 Dec 2014 15:27:52 GMT

←Older revision		Revision as of 15:27, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LIP_PSEAE LIP_PSEAE]] Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:48, 22 December 2014

OCA — Mon, 22 Dec 2014 18:48:15 GMT

←Older revision		Revision as of 18:48, 22 December 2014
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
-	[[Category: Dijkstra, B W.]]	+	[[Category: Dijkstra, B W]]
-	[[Category: Jaeger, K E.]]	+	[[Category: Jaeger, K E]]
-	[[Category: Lang, D A.]]	+	[[Category: Lang, D A]]
-	[[Category: Liebeton, K.]]	+	[[Category: Liebeton, K]]
-	[[Category: Nardini, M.]]	+	[[Category: Nardini, M]]
	[[Category: Alpha-beta hydrolase fold]]		[[Category: Alpha-beta hydrolase fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 08:49, 28 September 2014

OCA — Sun, 28 Sep 2014 08:49:13 GMT

←Older revision		Revision as of 08:49, 28 September 2014
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-	[[~~Image~~:1ex9.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE==
		+	<StructureSection load='1ex9' size='340' side='right' caption='[[1ex9]], [[Resolution\|resolution]] 2.54Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1EX9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OCP:OCTYL-PHOSPHINIC+ACID+1,2-BIS-OCTYLCARBAMOYLOXY-ETHYL+ESTER'>OCP</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ex9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ex9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ex9 RCSB], [http://www.ebi.ac.uk/pdbsum/1ex9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/1ex9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The x-ray structure of the lipase from Pseudomonas aeruginosa PAO1 has been determined at 2.54 A resolution. It is the first structure of a member of homology family I.1 of bacterial lipases. The structure shows a variant of the alpha/beta hydrolase fold, with Ser(82), Asp(229), and His(251) as the catalytic triad residues. Compared with the "canonical" alpha/beta hydrolase fold, the first two beta-strands and one alpha-helix (alphaE) are not present. The absence of helix alphaE allows the formation of a stabilizing intramolecular disulfide bridge. The loop containing His(251) is stabilized by an octahedrally coordinated calcium ion. On top of the active site a lid subdomain is in an open conformation, making the catalytic cleft accessible from the solvent region. A triacylglycerol analogue is covalently bound to Ser(82) in the active site, demonstrating the position of the oxyanion hole and of the three pockets that accommodate the sn-1, sn-2, and sn-3 fatty acid chains. The inhibited enzyme can be thought to mimic the structure of the tetrahedral intermediate that occurs during the acylation step of the reaction. Analysis of the binding mode of the inhibitor suggests that the size of the acyl pocket and the size and interactions of the sn-2 binding pocket are the predominant determinants of the regio- and enantio-preference of the enzyme.

-	~~{{STRUCTURE_1ex9\| PDB=1ex9 \| SCENE= }}~~	+	Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases.,Nardini M, Lang DA, Liebeton K, Jaeger KE, Dijkstra BW J Biol Chem. 2000 Oct 6;275(40):31219-25. PMID:10893416<ref>PMID:10893416</ref>

-	~~===CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP~~,~~SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10893416}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ex9]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA].~~	+

	==See Also==		==See Also==
	*[[Lipase\|Lipase]]		*[[Lipase\|Lipase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010893416<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]

OCA at 18:19, 20 October 2012

OCA — Sat, 20 Oct 2012 18:19:36 GMT

←Older revision		Revision as of 18:19, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1ex9]] is a 1 chain structure ~~of [[Lipase]]~~ with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA].	+	[[1ex9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EX9 OCA].

	==See Also==		==See Also==

OCA at 03:45, 27 July 2012

OCA — Fri, 27 Jul 2012 03:45:30 GMT

←Older revision		Revision as of 03:45, 27 July 2012
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	[[Image:1ex9.png\|left\|200px]]		[[Image:1ex9.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1ex9\| PDB=1ex9 \| SCENE= }}		{{STRUCTURE_1ex9\| PDB=1ex9 \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE===		===CRYSTAL STRUCTURE OF THE PSEUDOMONAS AERUGINOSA LIPASE COMPLEXED WITH RC-(RP,SP)-1,2-DIOCTYLCARBAMOYL-GLYCERO-3-O-OCTYLPHOSPHONATE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10893416}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10893416 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_10893416}}		{{ABSTRACT_PUBMED_10893416}}

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	==See Also==		==See Also==
-	*[[Lipase]]	+	*[[Lipase\|Lipase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10893416~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010893416</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
Line 34:		Line 23:
	[[Category: Nardini, M.]]		[[Category: Nardini, M.]]
	[[Category: Alpha-beta hydrolase fold]]		[[Category: Alpha-beta hydrolase fold]]
		+	[[Category: Hydrolase]]
	[[Category: Lipase]]		[[Category: Lipase]]
	[[Category: Phosphonate inhibitor]]		[[Category: Phosphonate inhibitor]]
	[[Category: Pseudomona]]		[[Category: Pseudomona]]