1f0c - Revision history

OCA at 07:09, 7 February 2024

OCA — Wed, 07 Feb 2024 07:09:18 GMT

←Older revision		Revision as of 07:09, 7 February 2024
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	<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cowpx Cowpx~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F0C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.26Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [https://pdbe.org/1f0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [https://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [https://pdbe.org/1f0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [https://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB]] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>	+	[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f0c ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f0c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Cowpox virus expresses the serpin CrmA (cytokine response modifier A) in order to avoid inflammatory and apoptotic responses of infected host cells. The targets of CrmA are members of the caspase family of proteases that either initiate the extrinsic pathway of apoptosis (caspases 8 and 10) or trigger activation of the pro-inflammatory cytokines interleukin-1beta and interleukin-18 (caspase 1). RESULTS: We have determined the structure of a cleaved form of CrmA to 2.26 A resolution. CrmA has the typical fold of a cleaved serpin, even though it lacks the N-terminal half of the A helix, the entire D helix, and a portion of the E helix that are present in all other known serpins. The reactive-site loop of CrmA was mutated to contain the optimal substrate recognition sequence for caspase 3; however, the mutation only marginally increased the ability of CrmA to inhibit caspase 3. Superposition of the reactive-site loop of alpha1-proteinase inhibitor on the cleaved CrmA structure provides a model for virgin CrmA that can be docked to caspase 1, but not to caspase 3. CONCLUSIONS: CrmA exemplifies viral economy, selective pressure having resulted in a 'minimal' serpin that lacks the regions not needed for structural integrity or inhibitory activity. The docking model provides an explanation for the selectivity of CrmA. Our demonstration that engineering optimal substrate recognition sequences into the CrmA reactive-site loop fails to generate a good caspase 3 inhibitor is consistent with the docking model.
-
-	Crystal structure of the apoptotic suppressor CrmA in its cleaved form.,Renatus M, Zhou Q, Stennicke HR, Snipas SJ, Turk D, Bankston LA, Liddington RC, Salvesen GS Structure. 2000 Jul 15;8(7):789-97. PMID:10903953<ref>PMID:10903953</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1f0c" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cowpx~~]]	+	[[Category: Cowpox virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bankston~~, L A~~]]	+	[[Category: Bankston LA]]
-	[[Category: Liddington~~, R C~~]]	+	[[Category: Liddington RC]]
-	[[Category: Renatus, M]]	+	[[Category: Renatus M]]
-	[[Category: Salvesen~~, G S~~]]	+	[[Category: Salvesen GS]]
-	[[Category: Snipas~~, S J~~]]	+	[[Category: Snipas SJ]]
-	[[Category: Stennicke~~, H R~~]]	+	[[Category: Stennicke HR]]
-	[[Category: Turk, D]]	+	[[Category: Turk D]]
-	[[Category: Zhou, Q]]	+	[[Category: Zhou Q]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Caspase inhibitor]]~~	+
-	~~[[Category: Protease inhibitor]]~~	+
-	~~[[Category: Serpin]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 07:17, 17 March 2021

OCA — Wed, 17 Mar 2021 07:17:25 GMT

←Older revision		Revision as of 07:17, 17 March 2021
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	<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cowpx Cowpx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1F0C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpx Cowpx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [~~http~~://pdbe.org/1f0c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [https://pdbe.org/1f0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [https://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB]] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>	+	[[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB]] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Serpin\|Serpin]]	+	*[[Serpin 3D structures\|Serpin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:43, 12 June 2019

OCA — Wed, 12 Jun 2019 06:43:09 GMT

←Older revision		Revision as of 06:43, 12 June 2019
Line 1:		Line 1:

	==STRUCTURE OF THE VIRAL SERPIN CRMA==		==STRUCTURE OF THE VIRAL SERPIN CRMA==
-	<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>	+	<StructureSection load='1f0c' size='340' side='right'caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpx Cowpx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://pdbe.org/1f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://pdbe.org/1f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f0c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f0c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cowpx]]
		+	[[Category: Large Structures]]
	[[Category: Bankston, L A]]		[[Category: Bankston, L A]]
	[[Category: Liddington, R C]]		[[Category: Liddington, R C]]

OCA at 03:54, 6 September 2017

OCA — Wed, 06 Sep 2017 03:54:44 GMT

←Older revision		Revision as of 03:54, 6 September 2017
Line 1:		Line 1:
		+
	==STRUCTURE OF THE VIRAL SERPIN CRMA==		==STRUCTURE OF THE VIRAL SERPIN CRMA==
	<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Cowpx Cowpx]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://pdbe.org/1f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://pdbe.org/1f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1f0c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f0c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cowpx]]
	[[Category: Bankston, L A]]		[[Category: Bankston, L A]]
	[[Category: Liddington, R C]]		[[Category: Liddington, R C]]

OCA at 09:08, 11 September 2015

OCA — Fri, 11 Sep 2015 09:08:24 GMT

←Older revision		Revision as of 09:08, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>		<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cowpox_virus Cowpox virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpx Cowpx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://pdbe.org/1f0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1f0c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serpin\|Serpin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cowpox virus~~]]	+	[[Category: Cowpx]]
	[[Category: Bankston, L A]]		[[Category: Bankston, L A]]
	[[Category: Liddington, R C]]		[[Category: Liddington, R C]]

OCA at 14:16, 25 December 2014

OCA — Thu, 25 Dec 2014 14:16:43 GMT

←Older revision		Revision as of 14:16, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB]] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:31, 22 December 2014

OCA — Mon, 22 Dec 2014 23:31:56 GMT

←Older revision		Revision as of 23:31, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Cowpox virus]]		[[Category: Cowpox virus]]
-	[[Category: Bankston, L A.]]	+	[[Category: Bankston, L A]]
-	[[Category: Liddington, R C.]]	+	[[Category: Liddington, R C]]
-	[[Category: Renatus, M.]]	+	[[Category: Renatus, M]]
-	[[Category: Salvesen, G S.]]	+	[[Category: Salvesen, G S]]
-	[[Category: Snipas, S J.]]	+	[[Category: Snipas, S J]]
-	[[Category: Stennicke, H R.]]	+	[[Category: Stennicke, H R]]
-	[[Category: Turk, D.]]	+	[[Category: Turk, D]]
-	[[Category: Zhou, Q.]]	+	[[Category: Zhou, Q]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Caspase inhibitor]]		[[Category: Caspase inhibitor]]

OCA at 13:14, 28 September 2014

OCA — Sun, 28 Sep 2014 13:14:55 GMT

←Older revision		Revision as of 13:14, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1f0c.~~png\|left~~\|200px]]	+	==STRUCTURE OF THE VIRAL SERPIN CRMA==
		+	<StructureSection load='1f0c' size='340' side='right' caption='[[1f0c]], [[Resolution\|resolution]] 2.26Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1F0C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f0c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1f0c RCSB], [http://www.ebi.ac.uk/pdbsum/1f0c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f0c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Cowpox virus expresses the serpin CrmA (cytokine response modifier A) in order to avoid inflammatory and apoptotic responses of infected host cells. The targets of CrmA are members of the caspase family of proteases that either initiate the extrinsic pathway of apoptosis (caspases 8 and 10) or trigger activation of the pro-inflammatory cytokines interleukin-1beta and interleukin-18 (caspase 1). RESULTS: We have determined the structure of a cleaved form of CrmA to 2.26 A resolution. CrmA has the typical fold of a cleaved serpin, even though it lacks the N-terminal half of the A helix, the entire D helix, and a portion of the E helix that are present in all other known serpins. The reactive-site loop of CrmA was mutated to contain the optimal substrate recognition sequence for caspase 3; however, the mutation only marginally increased the ability of CrmA to inhibit caspase 3. Superposition of the reactive-site loop of alpha1-proteinase inhibitor on the cleaved CrmA structure provides a model for virgin CrmA that can be docked to caspase 1, but not to caspase 3. CONCLUSIONS: CrmA exemplifies viral economy, selective pressure having resulted in a 'minimal' serpin that lacks the regions not needed for structural integrity or inhibitory activity. The docking model provides an explanation for the selectivity of CrmA. Our demonstration that engineering optimal substrate recognition sequences into the CrmA reactive-site loop fails to generate a good caspase 3 inhibitor is consistent with the docking model.

-	~~{{STRUCTURE_1f0c\| PDB=1f0c \| SCENE= }}~~	+	Crystal structure of the apoptotic suppressor CrmA in its cleaved form.,Renatus M, Zhou Q, Stennicke HR, Snipas SJ, Turk D, Bankston LA, Liddington RC, Salvesen GS Structure. 2000 Jul 15;8(7):789-97. PMID:10903953<ref>PMID:10903953</ref>

-	~~===STRUCTURE OF THE VIRAL SERPIN CRMA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~==About this Structure==~~	+	<references/>
-	~~[[1f0c]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cowpox_virus Cowpox virus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA].~~	+	__TOC__
-		+	</StructureSection>
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:010903953<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cowpox virus]]		[[Category: Cowpox virus]]
	[[Category: Bankston, L A.]]		[[Category: Bankston, L A.]]

OCA: Protected "1f0c" [edit=sysop:move=sysop]

OCA — Wed, 14 Nov 2012 07:36:13 GMT

Protected "1f0c" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:36, 14 November 2012

OCA at 07:36, 14 November 2012

OCA — Wed, 14 Nov 2012 07:36:10 GMT

←Older revision		Revision as of 07:36, 14 November 2012
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-	{{Seed}}
	[[Image:1f0c.png\|left\|200px]]		[[Image:1f0c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1f0c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1f0c\| PDB=1f0c \| SCENE= }}		{{STRUCTURE_1f0c\| PDB=1f0c \| SCENE= }}

	===STRUCTURE OF THE VIRAL SERPIN CRMA===		===STRUCTURE OF THE VIRAL SERPIN CRMA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10903953}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10903953 is the PubMed ID number.
-	-->
-	{{ABSTRACT_PUBMED_10903953}}

	==About this Structure==		==About this Structure==
-	~~1F0C~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Cow_pox_virus Cow pox~~ virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA].	+	[[1f0c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0C OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10903953~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010903953</ref><references group="xtra"/>
-	[[Category: ~~Cow pox~~ virus]]	+	[[Category: Cowpox virus]]
	[[Category: Bankston, L A.]]		[[Category: Bankston, L A.]]
	[[Category: Liddington, R C.]]		[[Category: Liddington, R C.]]
Line 37:		Line 24:
	[[Category: Protease inhibitor]]		[[Category: Protease inhibitor]]
	[[Category: Serpin]]		[[Category: Serpin]]
-		+	[[Category: Viral protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 18:27:51 2009''~~	+