1fjt - Revision history

OCA at 07:15, 7 February 2024

OCA — Wed, 07 Feb 2024 07:15:14 GMT

←Older revision		Revision as of 07:15, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [https://pdbe.org/1fjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [https://www.ebi.ac.uk/pdbsum/1fjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [https://pdbe.org/1fjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [https://www.ebi.ac.uk/pdbsum/1fjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.	+	[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Multiple Solvent Crystal Structures (MSCS) is a crystallographic technique to identify energetically favorable positions and orientations of small organic molecules on the surface of proteins. We determined the high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 50--70% acetone, 50--80% acetonitrile and 50 mM phenol. The structures of the protein in the aqueous-organic mixtures are essentially the same as the native enzyme and a number of solvent interaction sites were identified. The distribution of probe molecules shows clusters in the main specificity pocket of the active site and a buried subsite. Within the active site, we compared the experimentally determined solvent positions with predictions from two computational functional group mapping techniques, GRID and Multiple Copy Simultaneous Search (MCSS). The experimentally determined small molecule positions are consistent with the structures of known protein--ligand complexes of TLN.
-
-	Experimental and computational mapping of the binding surface of a crystalline protein.,English AC, Groom CR, Hubbard RE Protein Eng. 2001 Jan;14(1):47-59. PMID:11287678<ref>PMID:11287678</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Thermolysin 3D structures\|Thermolysin 3D structures]]		*[[Thermolysin 3D structures\|Thermolysin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Thermolysin]]~~	+	[[Category: English AC]]
-	[[Category: English~~, A C~~]]	+	[[Category: Groom CR]]
-	[[Category: Groom~~, C R~~]]	+	[[Category: Hubbard RE]]
-	[[Category: Hubbard~~, R E]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloproteinase]]~~	+
-	~~[[Category: Organic solvent~~]]	+

OCA at 11:10, 28 July 2021

OCA — Wed, 28 Jul 2021 11:10:35 GMT

←Older revision		Revision as of 11:10, 28 July 2021
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	<StructureSection load='1fjt' size='340' side='right'caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fjt' size='340' side='right'caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [~~http~~://pdbe.org/1fjt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fjt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [https://pdbe.org/1fjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [https://www.ebi.ac.uk/pdbsum/1fjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.	+	[[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Thermolysin\|Thermolysin]]	+	*[[Thermolysin 3D structures\|Thermolysin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:14, 23 October 2019

OCA — Wed, 23 Oct 2019 09:14:36 GMT

←Older revision		Revision as of 09:14, 23 October 2019
Line 1:		Line 1:

	==THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)==		==THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)==
-	<StructureSection load='1fjt' size='340' side='right' caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1fjt' size='340' side='right'caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJT FirstGlance]. <br>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
		+	[[Category: Large Structures]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]
	[[Category: English, A C]]		[[Category: English, A C]]

OCA at 09:33, 27 December 2017

OCA — Wed, 27 Dec 2017 09:33:37 GMT

←Older revision		Revision as of 09:33, 27 December 2017
Line 1:		Line 1:
		+
	==THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)==		==THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)==
	<StructureSection load='1fjt' size='340' side='right' caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1fjt' size='340' side='right' caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://pdbe.org/1fjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://pdbe.org/1fjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 19:13, 8 February 2016

OCA — Mon, 08 Feb 2016 19:13:23 GMT

←Older revision		Revision as of 19:13, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:34, 10 September 2015

OCA — Thu, 10 Sep 2015 14:34:40 GMT

←Older revision		Revision as of 14:34, 10 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://pdbe.org/1fjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 01:34, 25 December 2014

OCA — Thu, 25 Dec 2014 01:34:23 GMT

←Older revision		Revision as of 01:34, 25 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THER_BACTH THER_BACTH]] Extracellular zinc metalloprotease.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:17, 22 December 2014

OCA — Mon, 22 Dec 2014 23:17:43 GMT

←Older revision		Revision as of 23:17, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]
-	[[Category: English, A C.]]	+	[[Category: English, A C]]
-	[[Category: Groom, C R.]]	+	[[Category: Groom, C R]]
-	[[Category: Hubbard, R E.]]	+	[[Category: Hubbard, R E]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Metalloproteinase]]		[[Category: Metalloproteinase]]
	[[Category: Organic solvent]]		[[Category: Organic solvent]]

OCA at 12:59, 28 September 2014

OCA — Sun, 28 Sep 2014 12:59:20 GMT

←Older revision		Revision as of 12:59, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1fjt.~~png~~\|~~left~~\|200px]]	+	==THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)==
		+	<StructureSection load='1fjt' size='340' side='right' caption='[[1fjt]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FJT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=VAL:VALINE'>VAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tli\|1tli]], [[2tli\|2tli]], [[3tli\|3tli]], [[4tli\|4tli]], [[5tli\|5tli]], [[6tli\|6tli]], [[7tli\|7tli]], [[8tli\|8tli]], [[1fjo\|1fjo]], [[1fjq\|1fjq]], [[1fj3\|1fj3]], [[1fju\|1fju]], [[1fjv\|1fjv]], [[1fjw\|1fjw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fjt RCSB], [http://www.ebi.ac.uk/pdbsum/1fjt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fjt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multiple Solvent Crystal Structures (MSCS) is a crystallographic technique to identify energetically favorable positions and orientations of small organic molecules on the surface of proteins. We determined the high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 50--70% acetone, 50--80% acetonitrile and 50 mM phenol. The structures of the protein in the aqueous-organic mixtures are essentially the same as the native enzyme and a number of solvent interaction sites were identified. The distribution of probe molecules shows clusters in the main specificity pocket of the active site and a buried subsite. Within the active site, we compared the experimentally determined solvent positions with predictions from two computational functional group mapping techniques, GRID and Multiple Copy Simultaneous Search (MCSS). The experimentally determined small molecule positions are consistent with the structures of known protein--ligand complexes of TLN.

-	~~{{STRUCTURE_1fjt\| PDB=1fjt \| SCENE= }}~~	+	Experimental and computational mapping of the binding surface of a crystalline protein.,English AC, Groom CR, Hubbard RE Protein Eng. 2001 Jan;14(1):47-59. PMID:11287678<ref>PMID:11287678</ref>

-	~~===THERMOLYSIN (50% ACETONITRILE SOAKED CRYSTALS)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11287678}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1fjt]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA].~~	+

	==See Also==		==See Also==
	*[[Thermolysin\|Thermolysin]]		*[[Thermolysin\|Thermolysin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011287678<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus thermoproteolyticus]]		[[Category: Bacillus thermoproteolyticus]]
	[[Category: Thermolysin]]		[[Category: Thermolysin]]

OCA at 11:11, 20 October 2012

OCA — Sat, 20 Oct 2012 11:11:34 GMT

←Older revision		Revision as of 11:11, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1fjt]] is a 1 chain structure ~~of [[Thermolysin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA].	+	[[1fjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJT OCA].

	==See Also==		==See Also==