1fn8 - Revision history

OCA at 11:14, 27 March 2024

OCA — Wed, 27 Mar 2024 11:14:29 GMT

←Older revision		Revision as of 11:14, 27 March 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 0.81Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1try\|1try]], [[1gdn\|1gdn]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [https://pdbe.org/1fn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [https://pdbe.org/1fn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRYP_FUSOX TRYP_FUSOX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fn8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fn8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The X-ray structure of F. oxysporum trypsin has been determined at atomic resolution, revealing electron density in the binding site which was interpreted as a peptide bound in the sites S1, S2 and S3. The structure, which was initially determined at 1.07 A resolution and 283 K, has an Arg in the S1 specificity pocket. The study was extended to 0.81 A resolution at 100 K using crystals soaked in Arg, Lys and Gln to study in greater detail the binding at the S1 site. The electron density in the binding site was compared between the different structures and analysed in terms of partially occupied and overlapping components of peptide, solvent water and possibly other chemical moieties. Arg-soaked crystals reveal a density more detailed but similar to the original structure, with the Arg side chain visible in the S1 pocket and residual peptide density in the S2 and S3 sites. The density in the active site is complex and not fully interpreted. Lys at high concentrations displaces Arg in the S1 pocket, while some main-chain density remains in sites S2 and S3. Gln has been shown not to bind. The free peptide in the S1-S3 sites binds in a similar way to the binding loop of BPTI or the inhibitory domain of the Alzheimer's beta-protein precursor, with some differences in the S1 site.
-
-	Fusarium oxysporum trypsin at atomic resolution at 100 and 283 K: a study of ligand binding.,Rypniewski WR, Ostergaard PR, Norregaard-Madsen M, Dauter M, Wilson KS Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):8-19. PMID:11134922<ref>PMID:11134922</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fn8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Trypsin 3D structures\|Trypsin 3D structures]]		*[[Trypsin 3D structures\|Trypsin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Trypsin]]~~	+	[[Category: Dauter M]]
-	[[Category: Dauter, M]]	+	[[Category: Noerregaard-Madsen M]]
-	[[Category: Noerregaard-Madsen, M]]	+	[[Category: Oestergaard P]]
-	[[Category: Oestergaard, P]]	+	[[Category: Rypniewski WR]]
-	[[Category: Rypniewski~~, W R~~]]	+	[[Category: Wilson KS]]
-	[[Category: Wilson~~, K S]]~~	+
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-hydrolase substrate complex~~]]	+

OCA at 07:06, 24 March 2021

OCA — Wed, 24 Mar 2021 07:06:24 GMT

←Older revision		Revision as of 07:06, 24 March 2021
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	<StructureSection load='1fn8' size='340' side='right'caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>		<StructureSection load='1fn8' size='340' side='right'caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1try\|1try]], [[1gdn\|1gdn]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [~~http~~://pdbe.org/1fn8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fn8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [https://pdbe.org/1fn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:15, 19 June 2019

OCA — Wed, 19 Jun 2019 07:15:19 GMT

←Older revision		Revision as of 07:15, 19 June 2019
Line 1:		Line 1:

	==FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION==		==FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION==
-	<StructureSection load='1fn8' size='340' side='right' caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>	+	<StructureSection load='1fn8' size='340' side='right'caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Dauter, M]]		[[Category: Dauter, M]]

OCA at 10:39, 13 September 2017

OCA — Wed, 13 Sep 2017 10:39:58 GMT

←Older revision		Revision as of 10:39, 13 September 2017
Line 1:		Line 1:
		+
	==FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION==		==FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION==
	<StructureSection load='1fn8' size='340' side='right' caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>		<StructureSection load='1fn8' size='340' side='right' caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://pdbe.org/1fn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://pdbe.org/1fn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn8 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fn8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:47, 11 September 2015

OCA — Fri, 11 Sep 2015 10:47:49 GMT

←Older revision		Revision as of 10:47, 11 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://pdbe.org/1fn8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fn8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:43, 22 December 2014

OCA — Mon, 22 Dec 2014 18:43:44 GMT

←Older revision		Revision as of 18:43, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Dauter, M.]]	+	[[Category: Dauter, M]]
-	[[Category: Noerregaard-Madsen, M.]]	+	[[Category: Noerregaard-Madsen, M]]
-	[[Category: Oestergaard, P.]]	+	[[Category: Oestergaard, P]]
-	[[Category: Rypniewski, W R.]]	+	[[Category: Rypniewski, W R]]
-	[[Category: Wilson, K S.]]	+	[[Category: Wilson, K S]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Hydrolase-hydrolase substrate complex]]		[[Category: Hydrolase-hydrolase substrate complex]]

OCA at 08:45, 28 September 2014

OCA — Sun, 28 Sep 2014 08:45:24 GMT

←Older revision		Revision as of 08:45, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1fn8.~~png~~\|~~left~~\|200px]]	+	==FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION==
		+	<StructureSection load='1fn8' size='340' side='right' caption='[[1fn8]], [[Resolution\|resolution]] 0.81Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1try\|1try]], [[1gdn\|1gdn]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fn8 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The X-ray structure of F. oxysporum trypsin has been determined at atomic resolution, revealing electron density in the binding site which was interpreted as a peptide bound in the sites S1, S2 and S3. The structure, which was initially determined at 1.07 A resolution and 283 K, has an Arg in the S1 specificity pocket. The study was extended to 0.81 A resolution at 100 K using crystals soaked in Arg, Lys and Gln to study in greater detail the binding at the S1 site. The electron density in the binding site was compared between the different structures and analysed in terms of partially occupied and overlapping components of peptide, solvent water and possibly other chemical moieties. Arg-soaked crystals reveal a density more detailed but similar to the original structure, with the Arg side chain visible in the S1 pocket and residual peptide density in the S2 and S3 sites. The density in the active site is complex and not fully interpreted. Lys at high concentrations displaces Arg in the S1 pocket, while some main-chain density remains in sites S2 and S3. Gln has been shown not to bind. The free peptide in the S1-S3 sites binds in a similar way to the binding loop of BPTI or the inhibitory domain of the Alzheimer's beta-protein precursor, with some differences in the S1 site.

-	~~{{STRUCTURE_1fn8\| PDB=1fn8 \| SCENE= }}~~	+	Fusarium oxysporum trypsin at atomic resolution at 100 and 283 K: a study of ligand binding.,Rypniewski WR, Ostergaard PR, Norregaard-Madsen M, Dauter M, Wilson KS Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):8-19. PMID:11134922<ref>PMID:11134922</ref>

-	~~===FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11134922}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1fn8]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Fusarium_oxysporum Fusarium oxysporum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011134922<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 18:18, 20 October 2012

OCA — Sat, 20 Oct 2012 18:18:14 GMT

←Older revision		Revision as of 18:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1fn8]] is a 2 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA].	+	[[1fn8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA].

	==See Also==		==See Also==

OCA: Protected "1fn8" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 03:41:03 GMT

Protected "1fn8" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:41, 27 July 2012

OCA at 03:41, 27 July 2012

OCA — Fri, 27 Jul 2012 03:41:02 GMT

←Older revision		Revision as of 03:41, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1fn8.png\|left\|200px]]		[[Image:1fn8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1fn8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1fn8\| PDB=1fn8 \| SCENE= }}		{{STRUCTURE_1fn8\| PDB=1fn8 \| SCENE= }}

	===FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION===		===FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11134922}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11134922 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11134922}}		{{ABSTRACT_PUBMED_11134922}}

	==About this Structure==		==About this Structure==
-	~~1FN8~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA].	+	[[1fn8]] is a 2 chain structure of [[Trypsin]] with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN8 OCA].
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11134922~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011134922</ref><references group="xtra"/>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
Line 32:		Line 23:
	[[Category: Wilson, K S.]]		[[Category: Wilson, K S.]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
-		+	[[Category: Hydrolase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 20~~:~~16:38 2009''~~	+	[[Category: Hydrolase-hydrolase substrate complex]]