1fp7 - Revision history

OCA at 11:15, 27 March 2024

OCA — Wed, 27 Mar 2024 11:15:16 GMT

←Older revision		Revision as of 11:15, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1fp7' size='340' side='right'caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1fp7' size='340' side='right'caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35608 Atcc 35608~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1fpm\|1fpm]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [https://pdbe.org/1fp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1fp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fp7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [https://pdbe.org/1fp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1fp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fp7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FTHS_MOOTH FTHS_MOOTH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fp7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fp7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55-60 degrees C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. The crystal structures of complexes of FTHFS with cesium and potassium ions were examined and monovalent cation binding positions identified. Unexpectedly, NH(4)(+) and K(+), both of which are strongly activating ions, bind at a different site than a moderately activating ion, Cs(+), does. Neither binding site is located in the active site. The sites are 7 A apart, but in each of them, the side chain of Glu 98, which is conserved in all known bacterial FTHFS sequences, participates in metal ion binding. Other ligands in the Cs(+) binding site are four oxygen atoms of main chain carbonyls and water molecules. The K(+) and NH(4)(+) binding site includes the carboxylate of Asp132 in addition to Glu98. Mutant FTHFS's (E98Q, E98D, and E98S) were obtained and analyzed using differential scanning calorimetry to examine the effect of these mutations on the thermostability of the enzyme with and without added K(+) ions. The addition of 0.2 M K(+) ions to the wild-type enzyme resulted in a 10 degrees C increase in the thermal denaturation temperature. No significant increase was observed in E98D or E98S. The lack of a significant effect of monovalent cations on the stability of E98D and E98S indicates that this alteration of the binding site eliminates cation binding. The thermal denaturation temperature of E98Q was 3 degrees C higher than that of the wild-type enzyme in the absence of the cation, indicating that the removal of the unbalanced, buried charge of Glu98 stabilizes the enzyme. These results confirm that Glu98 is a crucial residue in the interaction of monovalent cations with FTHFS.
-
-	Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica.,Radfar R, Leaphart A, Brewer JM, Minor W, Odom JD, Dunlap RB, Lovell CR, Lebioda L Biochemistry. 2000 Nov 28;39(47):14481-6. PMID:11087401<ref>PMID:11087401</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fp7" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35608]]
-	[[Category: Formate--tetrahydrofolate ligase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brewer~~, J M~~]]	+	[[Category: Moorella thermoacetica]]
-	[[Category: Leaphart, A]]	+	[[Category: Brewer JM]]
-	[[Category: Minor, W]]	+	[[Category: Leaphart A]]
-	[[Category: Odom~~, J D~~]]	+	[[Category: Minor W]]
-	[[Category: Radfar, R]]	+	[[Category: Odom JD]]
-	~~[[Category: Ligase]]~~	+	[[Category: Radfar R]]
-	~~[[Category: Monovalent cation]]~~	+
-	~~[[Category: Tetramer]]~~	+
-	~~[[Category: Thermostable~~]]	+

OCA at 11:12, 28 July 2021

OCA — Wed, 28 Jul 2021 11:12:55 GMT

←Older revision		Revision as of 11:12, 28 July 2021
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	<StructureSection load='1fp7' size='340' side='right'caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1fp7' size='340' side='right'caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fpm\|1fpm]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [~~http~~://pdbe.org/1fp7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fp7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fp7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [https://pdbe.org/1fp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [https://www.ebi.ac.uk/pdbsum/1fp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fp7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:55, 23 October 2019

OCA — Wed, 23 Oct 2019 08:55:39 GMT

←Older revision		Revision as of 08:55, 23 October 2019
Line 1:		Line 1:

	==MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==		==MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==
-	<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='1fp7' size='340' side='right'caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
Line 34:		Line 34:
	[[Category: Atcc 35608]]		[[Category: Atcc 35608]]
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
		+	[[Category: Large Structures]]
	[[Category: Brewer, J M]]		[[Category: Brewer, J M]]
	[[Category: Leaphart, A]]		[[Category: Leaphart, A]]

OCA at 09:21, 27 December 2017

OCA — Wed, 27 Dec 2017 09:21:26 GMT

←Older revision		Revision as of 09:21, 27 December 2017
Line 1:		Line 1:
		+
	==MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==		==MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==
	<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://pdbe.org/1fp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://pdbe.org/1fp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fp7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/1fp7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/1fp7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:10, 7 February 2016

OCA — Sun, 07 Feb 2016 07:10:46 GMT

←Older revision		Revision as of 07:10, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fp7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:02, 9 September 2015

OCA — Wed, 09 Sep 2015 23:02:55 GMT

←Older revision		Revision as of 23:02, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Moorella_thermoacetica Moorella thermoacetica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35608 Atcc 35608]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://pdbe.org/1fp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fp7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 35608]]
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
-	[[Category: Moorella thermoacetica]]
	[[Category: Brewer, J M]]		[[Category: Brewer, J M]]
	[[Category: Leaphart, A]]		[[Category: Leaphart, A]]

OCA at 20:32, 22 December 2014

OCA — Mon, 22 Dec 2014 20:32:15 GMT

←Older revision		Revision as of 20:32, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
	[[Category: Moorella thermoacetica]]		[[Category: Moorella thermoacetica]]
-	[[Category: Brewer, J M.]]	+	[[Category: Brewer, J M]]
-	[[Category: Leaphart, A.]]	+	[[Category: Leaphart, A]]
-	[[Category: Minor, W.]]	+	[[Category: Minor, W]]
-	[[Category: Odom, J D.]]	+	[[Category: Odom, J D]]
-	[[Category: Radfar, R.]]	+	[[Category: Radfar, R]]
	[[Category: Ligase]]		[[Category: Ligase]]
	[[Category: Monovalent cation]]		[[Category: Monovalent cation]]
	[[Category: Tetramer]]		[[Category: Tetramer]]
	[[Category: Thermostable]]		[[Category: Thermostable]]

OCA at 10:23, 28 September 2014

OCA — Sun, 28 Sep 2014 10:23:37 GMT

←Older revision		Revision as of 10:23, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1fp7.~~png~~\|~~left~~\|200px]]	+	==MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==
		+	<StructureSection load='1fp7' size='340' side='right' caption='[[1fp7]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FP7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fpm\|1fpm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formate--tetrahydrofolate_ligase Formate--tetrahydrofolate ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.3 6.3.4.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fp7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fp7 RCSB], [http://www.ebi.ac.uk/pdbsum/1fp7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/1fp7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Formyltetrahydrofolate synthetase (FTHFS) from the thermophilic homoacetogen, Moorella thermoacetica, has an optimum temperature for activity of 55-60 degrees C and requires monovalent cations for both optimal activity and stabilization of tetrameric structure at higher temperatures. The crystal structures of complexes of FTHFS with cesium and potassium ions were examined and monovalent cation binding positions identified. Unexpectedly, NH(4)(+) and K(+), both of which are strongly activating ions, bind at a different site than a moderately activating ion, Cs(+), does. Neither binding site is located in the active site. The sites are 7 A apart, but in each of them, the side chain of Glu 98, which is conserved in all known bacterial FTHFS sequences, participates in metal ion binding. Other ligands in the Cs(+) binding site are four oxygen atoms of main chain carbonyls and water molecules. The K(+) and NH(4)(+) binding site includes the carboxylate of Asp132 in addition to Glu98. Mutant FTHFS's (E98Q, E98D, and E98S) were obtained and analyzed using differential scanning calorimetry to examine the effect of these mutations on the thermostability of the enzyme with and without added K(+) ions. The addition of 0.2 M K(+) ions to the wild-type enzyme resulted in a 10 degrees C increase in the thermal denaturation temperature. No significant increase was observed in E98D or E98S. The lack of a significant effect of monovalent cations on the stability of E98D and E98S indicates that this alteration of the binding site eliminates cation binding. The thermal denaturation temperature of E98Q was 3 degrees C higher than that of the wild-type enzyme in the absence of the cation, indicating that the removal of the unbalanced, buried charge of Glu98 stabilizes the enzyme. These results confirm that Glu98 is a crucial residue in the interaction of monovalent cations with FTHFS.

-	~~{{STRUCTURE_1fp7\| PDB=1fp7 \| SCENE= }}~~	+	Cation binding and thermostability of FTHFS monovalent cation binding sites and thermostability of N10-formyltetrahydrofolate synthetase from Moorella thermoacetica.,Radfar R, Leaphart A, Brewer JM, Minor W, Odom JD, Dunlap RB, Lovell CR, Lebioda L Biochemistry. 2000 Nov 28;39(47):14481-6. PMID:11087401<ref>PMID:11087401</ref>

-	~~===MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11087401}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1fp7]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Moorella_thermoacetica Moorella thermoacetica]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011087401<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
	[[Category: Moorella thermoacetica]]		[[Category: Moorella thermoacetica]]

OCA: Protected "1fp7" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 10:03:56 GMT

Protected "1fp7" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:03, 21 November 2012

OCA at 10:03, 21 November 2012

OCA — Wed, 21 Nov 2012 10:03:52 GMT

←Older revision		Revision as of 10:03, 21 November 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1fp7.png\|left\|200px]]		[[Image:1fp7.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1fp7", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1fp7\| PDB=1fp7 \| SCENE= }}		{{STRUCTURE_1fp7\| PDB=1fp7 \| SCENE= }}

	===MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA===		===MONOVALENT CATION BINDING SITES IN N10-FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11087401}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11087401 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11087401}}		{{ABSTRACT_PUBMED_11087401}}

	==About this Structure==		==About this Structure==
-	~~1FP7~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA].	+	[[1fp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP7 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11087401~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011087401</ref><references group="xtra"/>
	[[Category: Formate--tetrahydrofolate ligase]]		[[Category: Formate--tetrahydrofolate ligase]]
	[[Category: Moorella thermoacetica]]		[[Category: Moorella thermoacetica]]
Line 31:		Line 19:
	[[Category: Odom, J D.]]		[[Category: Odom, J D.]]
	[[Category: Radfar, R.]]		[[Category: Radfar, R.]]
		+	[[Category: Ligase]]
	[[Category: Monovalent cation]]		[[Category: Monovalent cation]]
	[[Category: Tetramer]]		[[Category: Tetramer]]
	[[Category: Thermostable]]		[[Category: Thermostable]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:10:55 2009''