1fuq - Revision history

OCA at 07:19, 7 February 2024

OCA — Wed, 07 Feb 2024 07:19:30 GMT

←Older revision		Revision as of 07:19, 7 February 2024
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	<StructureSection load='1fuq' size='340' side='right'caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1fuq' size='340' side='right'caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>~~, ~~<scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase]~~, ~~with EC number [https~~:~~//www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [https://pdbe.org/1fuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [https://www.ebi.ac.uk/pdbsum/1fuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [https://pdbe.org/1fuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [https://www.ebi.ac.uk/pdbsum/1fuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>	+	[https://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fuq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fuq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Fumarase C catalyzes the stereospecific interconversion of fumarate to L-malate as part of the metabolic citric acid or Kreb's cycle. The recent three-dimensional structure of fumarase C from Escherichia coli has identified a binding site for anions which is generated by side chains from three of the four subunits within the tetramer (Weaver et al., 1995). These same side chains are found in the three most highly conserved regions within the class II fumarase superfamily. The site was initially characterized by crystallographic studies through the binding of a heavy atom derivative, tungstate. A number of additional crystallographic structures using fumarase crystals with bound inhibitors and poor substrates have now been studied. The new structures have both confirmed the originally proposed active site, site A, and led to the discovery of a novel second binding site that is structurally nearby, site B. Site A utilizes a combination of residues, including H188, T187, K324, N326, T100, N141, S139, and S140, to form direct hydrogen bonds to each of the inhibitors. The A-site has been demonstrated by studying crystalline fumarase with the bound competitive inhibitors-citrate and 1,2,4,5-benzenetetracarboxylic acid. The crystal structure of fumarase C with beta-(trimethylsilyl)maleate, a cis substrate for fumarase, has led to the discovery of the second site or B-site. Sites A and B have different properties in terms of their three-dimensional structures. Site B, for example, is formed by atoms from only one of the subunits within the tetramer and mainly by atoms from a pi-helix between residues H129 through N135. The crystal structures show that the two locations are separated by approximately 12 A. A highly coordinated buried water molecule is also found at the active or A-site. The high-resolution crystal structures describe both sites, and atoms near the A-site are used to propose a likely enzyme/substrate complex.
-
-	Crystallographic studies of the catalytic and a second site in fumarase C from Escherichia coli.,Weaver T, Banaszak L Biochemistry. 1996 Nov 5;35(44):13955-65. PMID:8909293<ref>PMID:8909293</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Fumarate hydratase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Banaszak, L]]	+	[[Category: Banaszak L]]
-	[[Category: Weaver, T]]	+	[[Category: Weaver T]]
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Tricarboxylic acid cycle~~]]	+

OCA at 11:15, 28 July 2021

OCA — Wed, 28 Jul 2021 11:15:21 GMT

←Older revision		Revision as of 11:15, 28 July 2021
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	<StructureSection load='1fuq' size='340' side='right'caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1fuq' size='340' side='right'caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [~~http~~://pdbe.org/1fuq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fuq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [https://pdbe.org/1fuq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [https://www.ebi.ac.uk/pdbsum/1fuq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>	+	[[https://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:02, 23 October 2019

OCA — Wed, 23 Oct 2019 09:02:35 GMT

←Older revision		Revision as of 09:02, 23 October 2019
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	==FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID==		==FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID==
-	<StructureSection load='1fuq' size='340' side='right' caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1fuq' size='340' side='right'caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fumarase\|Fumarase]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Fumarate hydratase]]		[[Category: Fumarate hydratase]]
		+	[[Category: Large Structures]]
	[[Category: Banaszak, L]]		[[Category: Banaszak, L]]
	[[Category: Weaver, T]]		[[Category: Weaver, T]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 06:41, 3 January 2018

OCA — Wed, 03 Jan 2018 06:41:56 GMT

←Older revision		Revision as of 06:41, 3 January 2018
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		+
	==FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID==		==FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID==
	<StructureSection load='1fuq' size='340' side='right' caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1fuq' size='340' side='right' caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://pdbe.org/1fuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://pdbe.org/1fuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/1fuq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/1fuq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Fumarase\|Fumarase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:59, 8 February 2016

OCA — Mon, 08 Feb 2016 09:59:32 GMT

←Older revision		Revision as of 09:59, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fuq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:27, 10 September 2015

OCA — Thu, 10 Sep 2015 10:27:18 GMT

←Older revision		Revision as of 10:27, 10 September 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://pdbe.org/1fuq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fuq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 22:30, 24 December 2014

OCA — Wed, 24 Dec 2014 22:30:54 GMT

←Older revision		Revision as of 22:30, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FUMC_ECOLI FUMC_ECOLI]] Catalyzes the reversible addition of water to fumarate to give L-malate.<ref>PMID:1917897</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:51, 22 December 2014

OCA — Mon, 22 Dec 2014 18:51:39 GMT

←Older revision		Revision as of 18:51, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Fumarate hydratase]]		[[Category: Fumarate hydratase]]
-	[[Category: Banaszak, L.]]	+	[[Category: Banaszak, L]]
-	[[Category: Weaver, T.]]	+	[[Category: Weaver, T]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 05:51, 27 August 2014

OCA — Wed, 27 Aug 2014 05:51:12 GMT

←Older revision		Revision as of 05:51, 27 August 2014
Line 1:		Line 1:
-	[[~~Image~~:1fuq.~~png\|left~~\|200px]]	+	==FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID==
		+	<StructureSection load='1fuq' size='340' side='right' caption='[[1fuq]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FUQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SIF:3-TRIMETHYLSILYLSUCCINIC+ACID'>SIF</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fumarate_hydratase Fumarate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.2 4.2.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fuq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fuq RCSB], [http://www.ebi.ac.uk/pdbsum/1fuq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/1fuq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fumarase C catalyzes the stereospecific interconversion of fumarate to L-malate as part of the metabolic citric acid or Kreb's cycle. The recent three-dimensional structure of fumarase C from Escherichia coli has identified a binding site for anions which is generated by side chains from three of the four subunits within the tetramer (Weaver et al., 1995). These same side chains are found in the three most highly conserved regions within the class II fumarase superfamily. The site was initially characterized by crystallographic studies through the binding of a heavy atom derivative, tungstate. A number of additional crystallographic structures using fumarase crystals with bound inhibitors and poor substrates have now been studied. The new structures have both confirmed the originally proposed active site, site A, and led to the discovery of a novel second binding site that is structurally nearby, site B. Site A utilizes a combination of residues, including H188, T187, K324, N326, T100, N141, S139, and S140, to form direct hydrogen bonds to each of the inhibitors. The A-site has been demonstrated by studying crystalline fumarase with the bound competitive inhibitors-citrate and 1,2,4,5-benzenetetracarboxylic acid. The crystal structure of fumarase C with beta-(trimethylsilyl)maleate, a cis substrate for fumarase, has led to the discovery of the second site or B-site. Sites A and B have different properties in terms of their three-dimensional structures. Site B, for example, is formed by atoms from only one of the subunits within the tetramer and mainly by atoms from a pi-helix between residues H129 through N135. The crystal structures show that the two locations are separated by approximately 12 A. A highly coordinated buried water molecule is also found at the active or A-site. The high-resolution crystal structures describe both sites, and atoms near the A-site are used to propose a likely enzyme/substrate complex.

-	~~{{STRUCTURE_1fuq\| PDB=1fuq \| SCENE= }}~~	+	Crystallographic studies of the catalytic and a second site in fumarase C from Escherichia coli.,Weaver T, Banaszak L Biochemistry. 1996 Nov 5;35(44):13955-65. PMID:8909293<ref>PMID:8909293</ref>

-	~~===FUMARASE WITH BOUND PYROMELLITIC ACID===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8909293}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1fuq]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA].~~	+

	==See Also==		==See Also==
	*[[Fumarase\|Fumarase]]		*[[Fumarase\|Fumarase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008909293<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
-	[[Category: ~~Escherichia~~ coli]]	+	</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Fumarate hydratase]]		[[Category: Fumarate hydratase]]
	[[Category: Banaszak, L.]]		[[Category: Banaszak, L.]]

OCA at 09:47, 20 October 2012

OCA — Sat, 20 Oct 2012 09:47:05 GMT

←Older revision		Revision as of 09:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1fuq]] is a 2 chain structure ~~of [[Fumarase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA].	+	[[1fuq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUQ OCA].

	==See Also==		==See Also==