1fw2 - Revision history

OCA at 08:30, 22 May 2024

OCA — Wed, 22 May 2024 08:30:02 GMT

←Older revision		Revision as of 08:30, 22 May 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [https://pdbe.org/1fw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [https://pdbe.org/1fw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 09:38, 21 December 2022

OCA — Wed, 21 Dec 2022 09:38:52 GMT

←Older revision		Revision as of 09:38, 21 December 2022
Line 3:		Line 3:
	<StructureSection load='1fw2' size='340' side='right'caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1fw2' size='340' side='right'caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qd5\|1qd5]], [[1qd6\|1qd6]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [https://pdbe.org/1fw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [https://pdbe.org/1fw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PA1_ECOLI PA1_ECOLI]] Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.	+	[https://www.uniprot.org/uniprot/PA1_ECOLI PA1_ECOLI] Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dekker, N]]	+	[[Category: Dekker N]]
-	[[Category: Dijkstra~~, B W~~]]	+	[[Category: Dijkstra BW]]
-	[[Category: Egmond~~, M R~~]]	+	[[Category: Egmond MR]]
-	[[Category: Kalk~~, K H~~]]	+	[[Category: Kalk KH]]
-	[[Category: Kingma~~, R L~~]]	+	[[Category: Kingma RL]]
-	[[Category: Snijder~~, H J]]~~	+	[[Category: Snijder HJ]]
-	~~[[Category: Anti-parallel beta barrel dimer]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 11:15, 28 July 2021

OCA — Wed, 28 Jul 2021 11:15:54 GMT

←Older revision		Revision as of 11:15, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1fw2' size='340' side='right'caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1fw2' size='340' side='right'caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qd5\|1qd5]], [[1qd6\|1qd6]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [~~http~~://pdbe.org/1fw2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [https://pdbe.org/1fw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PA1_ECOLI PA1_ECOLI]] Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.	+	[[https://www.uniprot.org/uniprot/PA1_ECOLI PA1_ECOLI]] Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 08:23, 23 October 2019

OCA — Wed, 23 Oct 2019 08:23:52 GMT

←Older revision		Revision as of 08:23, 23 October 2019
Line 1:		Line 1:

	==OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI==		==OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI==
-	<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='1fw2' size='340' side='right'caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Dekker, N]]		[[Category: Dekker, N]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]

OCA at 06:13, 3 January 2018

OCA — Wed, 03 Jan 2018 06:13:31 GMT

←Older revision		Revision as of 06:13, 3 January 2018
Line 1:		Line 1:
		+
	==OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI==		==OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI==
	<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://pdbe.org/1fw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://pdbe.org/1fw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fw2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fw2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fw2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:35, 8 February 2016

OCA — Mon, 08 Feb 2016 03:35:56 GMT

←Older revision		Revision as of 03:35, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fw2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:29, 10 September 2015

OCA — Thu, 10 Sep 2015 07:29:15 GMT

←Older revision		Revision as of 07:29, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://pdbe.org/1fw2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1fw2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Dekker, N]]		[[Category: Dekker, N]]
	[[Category: Dijkstra, B W]]		[[Category: Dijkstra, B W]]

OCA at 20:17, 24 December 2014

OCA — Wed, 24 Dec 2014 20:17:38 GMT

←Older revision		Revision as of 20:17, 24 December 2014
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PA1_ECOLI PA1_ECOLI]] Has broad substrate specificity including hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities. Strong expression leads to outer membrane breakdown and cell death; is dormant in normal growing cells. Required for efficient secretion of bacteriocins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 22:32, 22 December 2014

OCA — Mon, 22 Dec 2014 22:32:54 GMT

←Older revision		Revision as of 22:32, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Dekker, N.]]	+	[[Category: Dekker, N]]
-	[[Category: Dijkstra, B W.]]	+	[[Category: Dijkstra, B W]]
-	[[Category: Egmond, M R.]]	+	[[Category: Egmond, M R]]
-	[[Category: Kalk, K H.]]	+	[[Category: Kalk, K H]]
-	[[Category: Kingma, R L.]]	+	[[Category: Kingma, R L]]
-	[[Category: Snijder, H J.]]	+	[[Category: Snijder, H J]]
	[[Category: Anti-parallel beta barrel dimer]]		[[Category: Anti-parallel beta barrel dimer]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 12:22, 28 September 2014

OCA — Sun, 28 Sep 2014 12:22:11 GMT

←Older revision		Revision as of 12:22, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1fw2.~~png~~\|~~left~~\|200px]]	+	==OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI==
		+	<StructureSection load='1fw2' size='340' side='right' caption='[[1fw2]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1fw2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FW2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qd5\|1qd5]], [[1qd6\|1qd6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(1) Phospholipase A(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.32 3.1.1.32] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fw2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fw2 RCSB], [http://www.ebi.ac.uk/pdbsum/1fw2 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/1fw2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Outer membrane phospholipase A (OMPLA) is an integral membrane enzyme that catalyses the hydrolysis of phospholipids. Enzymatic activity is regulated by reversible dimerisation and calcium-binding. We have investigated the role of calcium by X-ray crystallography. In monomeric OMPLA, one calcium ion binds between two external loops (L3L4 site) at 10 A from the active site. After dimerisation, a new calcium-binding site (catalytic site) is formed at the dimer interface in the active site of each molecule at 6 A from the L3L4 calcium site. The close spacing and the difference in calcium affinity of both sites suggests that the L3L4 site may function as a storage site for a calcium ion, which relocates to the catalytic site upon dimerisation. A sequence alignment demonstrates conservation of the catalytic calcium site but evolutionary variation of the L3L4 site. The residues in the dimer interface are conserved as well, suggesting that all outer membrane phospholipases require dimerisation and calcium in the catalytic site for activity. For this family of phospholipases, we have characterised a consensus sequence motif (YTQ-X(n)-G-X(2)-H-X-SNG) that contains conserved residues involved in dimerisation and catalysis.

-	~~{{STRUCTURE_1fw2\| PDB=1fw2 \| SCENE= }}~~	+	Structural investigations of calcium binding and its role in activity and activation of outer membrane phospholipase A from Escherichia coli.,Snijder HJ, Kingma RL, Kalk KH, Dekker N, Egmond MR, Dijkstra BW J Mol Biol. 2001 Jun 1;309(2):477-89. PMID:11371166<ref>PMID:11371166</ref>

-	~~===OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11371166}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1fw2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1FW2 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011371166<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Dekker, N.]]		[[Category: Dekker, N.]]