1g5j - Revision history

OCA at 08:30, 22 May 2024

OCA — Wed, 22 May 2024 08:30:43 GMT

←Older revision		Revision as of 08:30, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5j ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structure of the anti-apoptotic protein Bcl-xL complexed to a 25-residue peptide from the death promoting region of Bad was determined using NMR spectroscopy. Although the overall structure is similar to Bcl-xL bound to a 16-residue peptide from the Bak protein (Sattler et al., 1997), the Bad peptide forms additional interactions with Bcl-xL. However, based upon site-directed mutagenesis experiments, these additional contacts do not account for the increased affinity of the Bad 25-mer for Bcl-xL compared to the Bad 16-mer. Rather, the increased helix propensity of the Bad 25-mer is primarily responsible for its greater affinity for Bcl-xL. Based on this observation, a pair of 16-residue peptides were designed and synthesized that were predicted to have a high helix propensity while maintaining the interactions important for complexation with Bcl-xL. Both peptides showed an increase in helix propensity compared to the wild-type and exhibited an enhanced affinity for Bcl-xL.
		+
		+	Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies.,Petros AM, Nettesheim DG, Wang Y, Olejniczak ET, Meadows RP, Mack J, Swift K, Matayoshi ED, Zhang H, Thompson CB, Fesik SW Protein Sci. 2000 Dec;9(12):2528-34. PMID:11206074<ref>PMID:11206074</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:19, 27 March 2024

OCA — Wed, 27 Mar 2024 11:19:26 GMT

←Older revision		Revision as of 11:19, 27 March 2024
Line 1:		Line 1:

	==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==		==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==
-	<StructureSection load='1g5j' size='340' side='right'caption='[[1g5j~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1g5j' size='340' side='right'caption='[[1g5j]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [https://pdbe.org/1g5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [https://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [https://pdbe.org/1g5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [https://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN]] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> [[https://www.uniprot.org/uniprot/BAD_HUMAN BAD_HUMAN]] Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.	+	[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5j ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The three-dimensional structure of the anti-apoptotic protein Bcl-xL complexed to a 25-residue peptide from the death promoting region of Bad was determined using NMR spectroscopy. Although the overall structure is similar to Bcl-xL bound to a 16-residue peptide from the Bak protein (Sattler et al., 1997), the Bad peptide forms additional interactions with Bcl-xL. However, based upon site-directed mutagenesis experiments, these additional contacts do not account for the increased affinity of the Bad 25-mer for Bcl-xL compared to the Bad 16-mer. Rather, the increased helix propensity of the Bad 25-mer is primarily responsible for its greater affinity for Bcl-xL. Based on this observation, a pair of 16-residue peptides were designed and synthesized that were predicted to have a high helix propensity while maintaining the interactions important for complexation with Bcl-xL. Both peptides showed an increase in helix propensity compared to the wild-type and exhibited an enhanced affinity for Bcl-xL.
-
-	Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies.,Petros AM, Nettesheim DG, Wang Y, Olejniczak ET, Meadows RP, Mack J, Swift K, Matayoshi ED, Zhang H, Thompson CB, Fesik SW Protein Sci. 2000 Dec;9(12):2528-34. PMID:11206074<ref>PMID:11206074</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fesik~~, S W~~]]	+	[[Category: Fesik SW]]
-	[[Category: Mack, J]]	+	[[Category: Mack J]]
-	[[Category: Matayoshi~~, E D~~]]	+	[[Category: Matayoshi ED]]
-	[[Category: Meadows~~, R P~~]]	+	[[Category: Meadows RP]]
-	[[Category: Nettesheim~~, D G~~]]	+	[[Category: Nettesheim DG]]
-	[[Category: Olejniczak~~, E T~~]]	+	[[Category: Olejniczak ET]]
-	[[Category: Petros~~, A M~~]]	+	[[Category: Petros AM]]
-	[[Category: Swift, K]]	+	[[Category: Swift K]]
-	[[Category: Thompson~~, C B~~]]	+	[[Category: Thompson CB]]
-	[[Category: Wang, Y]]	+	[[Category: Wang Y]]
-	[[Category: Zhang, H]]	+	[[Category: Zhang H]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Complex~~]]	+

OCA at 14:16, 27 October 2021

OCA — Wed, 27 Oct 2021 14:16:38 GMT

←Older revision		Revision as of 14:16, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1g5j' size='340' side='right'caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1g5j' size='340' side='right'caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1G5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [~~http~~://pdbe.org/1g5j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [https://pdbe.org/1g5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [https://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN]] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> [[~~http~~://www.uniprot.org/uniprot/BAD_HUMAN BAD_HUMAN]] Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.	+	[[https://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN]] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> [[https://www.uniprot.org/uniprot/BAD_HUMAN BAD_HUMAN]] Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:27, 18 November 2020

OCA — Wed, 18 Nov 2020 10:27:51 GMT

←Older revision		Revision as of 10:27, 18 November 2020
Line 1:		Line 1:

	==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==		==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==
-	<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1g5j' size='340' side='right'caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1G5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://pdbe.org/1g5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://pdbe.org/1g5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[B-cell lymphoma proteins 3D structures\|B-cell lymphoma proteins 3D structures]]
		+	*[[Horseradish peroxidase\|Horseradish peroxidase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Fesik, S W]]		[[Category: Fesik, S W]]
	[[Category: Mack, J]]		[[Category: Mack, J]]

OCA at 06:37, 3 January 2018

OCA — Wed, 03 Jan 2018 06:37:33 GMT

←Older revision		Revision as of 06:37, 3 January 2018
Line 1:		Line 1:
		+
	==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==		==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==
	<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://pdbe.org/1g5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://pdbe.org/1g5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Bcl-2\|Bcl-2]]
-	*[[Horseradish peroxidase\|Horseradish peroxidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 00:04, 10 February 2016

OCA — Wed, 10 Feb 2016 00:04:30 GMT

←Older revision		Revision as of 00:04, 10 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:23, 11 September 2015

OCA — Fri, 11 Sep 2015 03:23:01 GMT

←Older revision		Revision as of 03:23, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G5J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://pdbe.org/1g5j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1g5j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Fesik, S W]]		[[Category: Fesik, S W]]
	[[Category: Mack, J]]		[[Category: Mack, J]]

OCA at 10:25, 25 December 2014

OCA — Thu, 25 Dec 2014 10:25:55 GMT

←Older revision		Revision as of 10:25, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/B2CL1_HUMAN B2CL1_HUMAN]] Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> Isoform Bcl-X(S) promotes apoptosis.<ref>PMID:19917720</ref> <ref>PMID:21840391</ref> [[http://www.uniprot.org/uniprot/BAD_HUMAN BAD_HUMAN]] Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 23:46, 22 December 2014

OCA — Mon, 22 Dec 2014 23:46:38 GMT

←Older revision		Revision as of 23:46, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G5J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Fesik, S W.]]	+	[[Category: Fesik, S W]]
-	[[Category: Mack, J.]]	+	[[Category: Mack, J]]
-	[[Category: Matayoshi, E D.]]	+	[[Category: Matayoshi, E D]]
-	[[Category: Meadows, R P.]]	+	[[Category: Meadows, R P]]
-	[[Category: Nettesheim, D G.]]	+	[[Category: Nettesheim, D G]]
-	[[Category: Olejniczak, E T.]]	+	[[Category: Olejniczak, E T]]
-	[[Category: Petros, A M.]]	+	[[Category: Petros, A M]]
-	[[Category: Swift, K.]]	+	[[Category: Swift, K]]
-	[[Category: Thompson, C B.]]	+	[[Category: Thompson, C B]]
-	[[Category: Wang, Y.]]	+	[[Category: Wang, Y]]
-	[[Category: Zhang, H.]]	+	[[Category: Zhang, H]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Complex]]		[[Category: Complex]]

OCA at 13:30, 28 September 2014

OCA — Sun, 28 Sep 2014 13:30:30 GMT

←Older revision		Revision as of 13:30, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1g5j.~~png~~\|~~left~~\|200px]]	+	==COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD==
		+	<StructureSection load='1g5j' size='340' side='right' caption='[[1g5j]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1g5j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G5J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bxl\|1bxl]], [[1g5m\|1g5m]], [[1g5o\|1g5o]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BCLX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g5j RCSB], [http://www.ebi.ac.uk/pdbsum/1g5j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structure of the anti-apoptotic protein Bcl-xL complexed to a 25-residue peptide from the death promoting region of Bad was determined using NMR spectroscopy. Although the overall structure is similar to Bcl-xL bound to a 16-residue peptide from the Bak protein (Sattler et al., 1997), the Bad peptide forms additional interactions with Bcl-xL. However, based upon site-directed mutagenesis experiments, these additional contacts do not account for the increased affinity of the Bad 25-mer for Bcl-xL compared to the Bad 16-mer. Rather, the increased helix propensity of the Bad 25-mer is primarily responsible for its greater affinity for Bcl-xL. Based on this observation, a pair of 16-residue peptides were designed and synthesized that were predicted to have a high helix propensity while maintaining the interactions important for complexation with Bcl-xL. Both peptides showed an increase in helix propensity compared to the wild-type and exhibited an enhanced affinity for Bcl-xL.

-	~~{{STRUCTURE_1g5j\| PDB=1g5j \| SCENE= }}~~	+	Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies.,Petros AM, Nettesheim DG, Wang Y, Olejniczak ET, Meadows RP, Mack J, Swift K, Matayoshi ED, Zhang H, Thompson CB, Fesik SW Protein Sci. 2000 Dec;9(12):2528-34. PMID:11206074<ref>PMID:11206074</ref>

-	~~===COMPLEX OF BCL-XL WITH PEPTIDE FROM BAD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11206074}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1g5j]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1G5J OCA].~~	+

	==See Also==		==See Also==
	*[[Bcl-2\|Bcl-2]]		*[[Bcl-2\|Bcl-2]]
	*[[Horseradish peroxidase\|Horseradish peroxidase]]		*[[Horseradish peroxidase\|Horseradish peroxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011206074<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Fesik, S W.]]		[[Category: Fesik, S W.]]