1g6c - Revision history

OCA at 06:08, 9 August 2023

OCA — Wed, 09 Aug 2023 06:08:38 GMT

←Older revision		Revision as of 06:08, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [https://pdbe.org/1g6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [https://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [https://pdbe.org/1g6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [https://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>	+	[https://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Thiamine-phosphate diphosphorylase]]~~	+	[[Category: Begley TP]]
-	[[Category: Begley~~, T P~~]]	+	[[Category: Campobasso N]]
-	[[Category: Campobasso, N]]	+	[[Category: Chiu H-J]]
-	[[Category: Chiu, H J]]	+	[[Category: Ealick SE]]
-	[[Category: Ealick~~, S E~~]]	+	[[Category: Peapus DH]]
-	[[Category: Peapus~~, D H~~]]	+	[[Category: Reddick JJ]]
-	[[Category: Reddick~~, J J]]~~	+
-	~~[[Category: Thiamin biosynthesis]]~~	+
-	~~[[Category: Tim barrel]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 14:16, 27 October 2021

OCA — Wed, 27 Oct 2021 14:16:53 GMT

←Older revision		Revision as of 14:16, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [https://pdbe.org/1g6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [https://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [https://pdbe.org/1g6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [https://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Vibrio subtilis ehrenberg 1835]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]

OCA at 07:12, 24 March 2021

OCA — Wed, 24 Mar 2021 07:12:18 GMT

←Older revision		Revision as of 07:12, 24 March 2021
Line 3:		Line 3:
	<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1G6C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [~~http~~://pdbe.org/1g6c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [https://pdbe.org/1g6c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [https://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>	+	[[https://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:09, 19 June 2019

OCA — Wed, 19 Jun 2019 07:09:32 GMT

←Older revision		Revision as of 07:09, 19 June 2019
Line 1:		Line 1:

	==THIAMIN PHOSPHATE SYNTHASE==		==THIAMIN PHOSPHATE SYNTHASE==
-	<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='1g6c' size='340' side='right'caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1g6c" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1g6c" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thiamin phosphate synthase\|Thiamin phosphate synthase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]

OCA at 10:58, 13 September 2017

OCA — Wed, 13 Sep 2017 10:58:31 GMT

←Older revision		Revision as of 10:58, 13 September 2017
Line 1:		Line 1:
		+
	==THIAMIN PHOSPHATE SYNTHASE==		==THIAMIN PHOSPHATE SYNTHASE==
	<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://pdbe.org/1g6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://pdbe.org/1g6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g6c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus globigii migula 1900]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]

OCA at 09:57, 11 September 2015

OCA — Fri, 11 Sep 2015 09:57:44 GMT

←Older revision		Revision as of 09:57, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://pdbe.org/1g6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1g6c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]

OCA at 14:49, 25 December 2014

OCA — Thu, 25 Dec 2014 14:49:59 GMT

←Older revision		Revision as of 14:49, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THIE_BACSU THIE_BACSU]] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.<ref>PMID:9139923</ref> <ref>PMID:11513588</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 00:25, 23 December 2014

OCA — Tue, 23 Dec 2014 00:25:16 GMT

←Older revision		Revision as of 00:25, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]
-	[[Category: Begley, T P.]]	+	[[Category: Begley, T P]]
-	[[Category: Campobasso, N.]]	+	[[Category: Campobasso, N]]
-	[[Category: Chiu, H J.]]	+	[[Category: Chiu, H J]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Peapus, D H.]]	+	[[Category: Peapus, D H]]
-	[[Category: Reddick, J J.]]	+	[[Category: Reddick, J J]]
	[[Category: Thiamin biosynthesis]]		[[Category: Thiamin biosynthesis]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:12, 28 September 2014

OCA — Sun, 28 Sep 2014 14:12:16 GMT

←Older revision		Revision as of 14:12, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1g6c.~~png~~\|~~left~~\|200px]]	+	==THIAMIN PHOSPHATE SYNTHASE==
		+	<StructureSection load='1g6c' size='340' side='right' caption='[[1g6c]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1g6c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G6C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IFP:2-TRIFLUOROMETHYL-5-METHYLENE-5H-PYRIMIDIN-4-YLIDENEAMINE'>IFP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=TZP:4-METHYL-5-HYDROXYETHYLTHIAZOLE+PHOSPHATE'>TZP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2tps\|2tps]], [[1g4s\|1g4s]], [[1g4p\|1g4p]], [[1g4t\|1g4t]], [[1g4e\|1g4e]], [[1g67\|1g67]], [[1g69\|1g69]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Thiamine-phosphate_diphosphorylase Thiamine-phosphate diphosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.3 2.5.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g6c RCSB], [http://www.ebi.ac.uk/pdbsum/1g6c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thiamin phosphate synthase catalyzes the formation of thiamin phosphate from 4-amino-5-(hydroxymethyl)-2-methylpyrimidine pyrophosphate and 5-(hydroxyethyl)-4-methylthiazole phosphate. Several lines of evidence suggest that the reaction proceeds via a dissociative mechanism. The previously determined crystal structure of thiamin phosphate synthase in complex with the reaction products, thiamin phosphate and magnesium pyrophosphate, provided a view of the active site and suggested a number of additional experiments. We report here seven new crystal structures primarily involving crystals of S130A thiamin phosphate synthase soaked in solutions containing substrates or products. We prepared S130A thiamin phosphate synthase with the intent of characterizing the enzyme-substrate complex. Surprisingly, in three thiamin phosphate synthase structures, the active site density cannot be modeled as either substrates or products. For these structures, the best fit to the electron density is provided by a model that consists of independent pyrimidine, pyrophosphate, and thiazole phosphate fragments, consistent with a carbenium ion intermediate. The resulting carbenium ion is likely to be further stabilized by proton transfer from the pyrimidine amino group to the pyrophosphate to give the pyrimidine iminemethide, which we believe is the species that is observed in the crystal structures.

-	~~{{STRUCTURE_1g6c\| PDB=1g6c \| SCENE= }}~~	+	Structural characterization of the enzyme-substrate, enzyme-intermediate, and enzyme-product complexes of thiamin phosphate synthase.,Peapus DH, Chiu HJ, Campobasso N, Reddick JJ, Begley TP, Ealick SE Biochemistry. 2001 Aug 28;40(34):10103-14. PMID:11513589<ref>PMID:11513589</ref>

-	~~===THIAMIN PHOSPHATE SYNTHASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11513589}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1g6c]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1G6C OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011513589<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Thiamine-phosphate diphosphorylase]]		[[Category: Thiamine-phosphate diphosphorylase]]

OCA: Protected "1g6c" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:53:51 GMT

Protected "1g6c" [edit=sysop:move=sysop]

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Revision as of 15:53, 21 November 2012