1gc9 - Revision history

OCA at 23:29, 27 December 2023

OCA — Wed, 27 Dec 2023 23:29:41 GMT

←Older revision		Revision as of 23:29, 27 December 2023
Line 3:		Line 3:
	<StructureSection load='1gc9' size='340' side='right'caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1gc9' size='340' side='right'caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">LEUB ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=300852 THET8])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [https://pdbe.org/1gc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [https://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [https://pdbe.org/1gc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [https://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8]] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]	+	[https://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 3-isopropylmalate dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet8~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: Akanuma, S]]	+	[[Category: Akanuma S]]
-	[[Category: Moriyama, H]]	+	[[Category: Moriyama H]]
-	[[Category: Oshima, T]]	+	[[Category: Oshima T]]
-	[[Category: Qu, C]]	+	[[Category: Qu C]]
-	[[Category: Tanaka, N]]	+	[[Category: Tanaka N]]
-	~~[[Category: Decarboxylation]]~~	+
-	~~[[Category: Dehydrogenation]]~~	+
-	~~[[Category: Imdh]]~~	+
-	~~[[Category: Ipmdh]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Thermostability~~]]	+

OCA at 06:18, 10 November 2021

OCA — Wed, 10 Nov 2021 06:18:13 GMT

←Older revision		Revision as of 06:18, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='1gc9' size='340' side='right'caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1gc9' size='340' side='right'caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [~~http~~://pdbe.org/1gc9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [https://pdbe.org/1gc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [https://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8]] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]	+	[[https://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8]] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:29, 18 November 2020

OCA — Wed, 18 Nov 2020 10:29:41 GMT

←Older revision		Revision as of 10:29, 18 November 2020
Line 1:		Line 1:

	==THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY==		==THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY==
-	<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1gc9' size='340' side='right'caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://pdbe.org/1gc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://pdbe.org/1gc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1gc9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gc9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Isopropylmalate dehydrogenase\|Isopropylmalate dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: 3-isopropylmalate dehydrogenase]]		[[Category: 3-isopropylmalate dehydrogenase]]
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Akanuma, S]]		[[Category: Akanuma, S]]

OCA at 06:15, 3 January 2018

OCA — Wed, 03 Jan 2018 06:15:38 GMT

←Older revision		Revision as of 06:15, 3 January 2018
Line 1:		Line 1:
		+
	==THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY==		==THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY==
	<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://pdbe.org/1gc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://pdbe.org/1gc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gc9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gc9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gc9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1gc9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gc9" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Isopropylmalate dehydrogenase\|Isopropylmalate dehydrogenase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:24, 8 February 2016

OCA — Mon, 08 Feb 2016 05:24:44 GMT

←Older revision		Revision as of 05:24, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gc9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:19, 10 September 2015

OCA — Thu, 10 Sep 2015 08:19:03 GMT

←Older revision		Revision as of 08:19, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://pdbe.org/1gc9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEU3_THET8 LEU3_THET8]] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01033]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gc9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: 3-isopropylmalate dehydrogenase]]		[[Category: 3-isopropylmalate dehydrogenase]]
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Akanuma, S]]		[[Category: Akanuma, S]]
	[[Category: Moriyama, H]]		[[Category: Moriyama, H]]

OCA at 23:23, 22 December 2014

OCA — Mon, 22 Dec 2014 23:23:02 GMT

←Older revision		Revision as of 23:23, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: 3-isopropylmalate dehydrogenase]]		[[Category: 3-isopropylmalate dehydrogenase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Akanuma, S.]]	+	[[Category: Akanuma, S]]
-	[[Category: Moriyama, H.]]	+	[[Category: Moriyama, H]]
-	[[Category: Oshima, T.]]	+	[[Category: Oshima, T]]
-	[[Category: Qu, C.]]	+	[[Category: Qu, C]]
-	[[Category: Tanaka, N.]]	+	[[Category: Tanaka, N]]
	[[Category: Decarboxylation]]		[[Category: Decarboxylation]]
	[[Category: Dehydrogenation]]		[[Category: Dehydrogenation]]

OCA at 13:05, 28 September 2014

OCA — Sun, 28 Sep 2014 13:05:44 GMT

←Older revision		Revision as of 13:05, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1gc9.~~png~~\|~~left~~\|200px]]	+	==THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY==
		+	<StructureSection load='1gc9' size='340' side='right' caption='[[1gc9]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GC9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ipd\|1ipd]], [[1osj\|1osj]], [[1gc8\|1gc8]], [[1dpz\|1dpz]], [[1dr0\|1dr0]], [[1dr8\|1dr8]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LEUB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-isopropylmalate_dehydrogenase 3-isopropylmalate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.85 1.1.1.85] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gc9 RCSB], [http://www.ebi.ac.uk/pdbsum/1gc9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gc9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The relationship between the structure and the thermostability of the 3-isopropylmalate dehydrogenase from Thermus thermophilus was studied by site-directed mutation of a single Ala residue located at the domain interface. The crystal structures of three mutant enzymes, replacing Ala172 with Gly, Val and Phe, were successfully determined at 2.3, 2.2 and 2.5 A resolution, respectively. Substitution of Ala172 by relatively 'short' residues (Gly, Val or Ile) enlarges or narrows the cavity in the vicinity of the C(beta) atom of Ala172 and the thermostablity of the enzyme shows a good correlation with the hydrophobicity of the substituted residues. Substitution of Ala172 by the 'longer' residues Leu or Phe causes a rearrangement of the domain structure, which leads to a higher thermostability of the enzymes than that expected from the hydrophobicity of the substituted residues. Mutation of Ala172 to negatively charged residues gave an unexpected result: the melting temperature of the Asp mutant enzyme was reduced by 2.7 K while that of the Glu mutant increased by 1.8 K. Molecular-modelling studies indicated that the glutamate side chain was sufficiently long that it did not act as a buried charge as did the aspartate, but instead protruded to the outside of the hydrophobic cavity and contributed to the stability of the enzyme by enhancing the packing of the local side chains and forming an extra salt bridge with the side chain of Lys175.

-	~~{{STRUCTURE_1gc9\| PDB=1gc9 \| SCENE= }}~~	+	Design, X-ray crystallography, molecular modelling and thermal stability studies of mutant enzymes at site 172 of 3-isopropylmalate dehydrogenase from Thermus thermophilus.,Qu C, Akanuma S, Tanaka N, Moriyama H, Oshima T Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):225-32. PMID:11173468<ref>PMID:11173468</ref>

-	~~===THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO GLY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11173468}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1gc9]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus Thermus thermophilus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA].~~	+

	==See Also==		==See Also==
	*[[Isopropylmalate dehydrogenase\|Isopropylmalate dehydrogenase]]		*[[Isopropylmalate dehydrogenase\|Isopropylmalate dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011173468<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: 3-isopropylmalate dehydrogenase]]		[[Category: 3-isopropylmalate dehydrogenase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]

OCA at 09:03, 20 October 2012

OCA — Sat, 20 Oct 2012 09:03:41 GMT

←Older revision		Revision as of 09:03, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1gc9]] is a 1 chain structure ~~of [[Isopropylmalate dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA].	+	[[1gc9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GC9 OCA].

	==See Also==		==See Also==

OCA: Protected "1gc9" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 21:39:08 GMT

Protected "1gc9" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:39, 25 July 2012