1gjy - Revision history

OCA at 08:48, 9 May 2024

OCA — Thu, 09 May 2024 08:48:56 GMT

←Older revision		Revision as of 08:48, 9 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sorcin is a 21.6 kDa calcium binding protein, expressed in a number of mammalian tissues that belongs to the small, recently identified penta-EF-hand (PEF) family. Like all members of this family, sorcin undergoes a Ca2+-dependent translocation from cytosol to membranes where it binds to target proteins. For sorcin, the targets differ in different tissues, indicating that it takes part in a number of Ca2+-regulated processes. The sorcin monomer is organized in two domains like in all PEF proteins: a flexible, hydrophobic, glycine-rich N-terminal region and a calcium binding C-terminal domain. In vitro, the PEF proteins are dimeric in their Ca2+-free form, but have a marked tendency to precipitate when bound to calcium. Stabilization of the dimeric structure is achieved by pairing of the uneven EF-hand, EF5. Sorcin can also form tetramers at acid pH.The sorcin calcium binding domain (SCBD, residues 33-198) expressed in Escherichia coli was crystallized in the Ca2+-free form. The structure was solved by molecular replacement and was refined to 2.2 A with a crystallographic R-factor of 22.4 %. Interestingly, the asymmetric unit contains two dimers.The structure of the SCBD leads to a model that explains the solution properties and describes the Ca2+-induced conformational changes. Phosphorylation studies show that the N-terminal domain hinders phosphorylation of SCBD, i.e. the rate of phosphorylation increased twofold in the absence of the N-terminal region. In addition, previous fluorescence studies indicated that hydrophobic residues are exposed to solvent upon Ca2+ binding to full-length sorcin. The model accounts for these data by proposing that Ca2+ binding weakens the interactions between the two domains and leads to their reorientation, which exposes hydrophobic regions facilitating the Ca2+-dependent binding to target proteins at or near membranes.
		+
		+	The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein.,Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E J Mol Biol. 2002 Mar 29;317(3):447-58. PMID:11922676<ref>PMID:11922676</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1gjy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:21, 27 March 2024

OCA — Wed, 27 Mar 2024 11:21:54 GMT

←Older revision		Revision as of 11:21, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1gjy' size='340' side='right'caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1gjy' size='340' side='right'caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chinese_hamster Chinese hamster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GJY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [https://pdbe.org/1gjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [https://www.ebi.ac.uk/pdbsum/1gjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [https://pdbe.org/1gjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [https://www.ebi.ac.uk/pdbsum/1gjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SORCN_CRIGR SORCN_CRIGR] Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the sarcoplasmic reticulum in the heart. Modulates the activity of RYR2 calcium channels.<ref>PMID:15754088</ref> <ref>PMID:17029407</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Sorcin is a 21.6 kDa calcium binding protein, expressed in a number of mammalian tissues that belongs to the small, recently identified penta-EF-hand (PEF) family. Like all members of this family, sorcin undergoes a Ca2+-dependent translocation from cytosol to membranes where it binds to target proteins. For sorcin, the targets differ in different tissues, indicating that it takes part in a number of Ca2+-regulated processes. The sorcin monomer is organized in two domains like in all PEF proteins: a flexible, hydrophobic, glycine-rich N-terminal region and a calcium binding C-terminal domain. In vitro, the PEF proteins are dimeric in their Ca2+-free form, but have a marked tendency to precipitate when bound to calcium. Stabilization of the dimeric structure is achieved by pairing of the uneven EF-hand, EF5. Sorcin can also form tetramers at acid pH.The sorcin calcium binding domain (SCBD, residues 33-198) expressed in Escherichia coli was crystallized in the Ca2+-free form. The structure was solved by molecular replacement and was refined to 2.2 A with a crystallographic R-factor of 22.4 %. Interestingly, the asymmetric unit contains two dimers.The structure of the SCBD leads to a model that explains the solution properties and describes the Ca2+-induced conformational changes. Phosphorylation studies show that the N-terminal domain hinders phosphorylation of SCBD, i.e. the rate of phosphorylation increased twofold in the absence of the N-terminal region. In addition, previous fluorescence studies indicated that hydrophobic residues are exposed to solvent upon Ca2+ binding to full-length sorcin. The model accounts for these data by proposing that Ca2+ binding weakens the interactions between the two domains and leads to their reorientation, which exposes hydrophobic regions facilitating the Ca2+-dependent binding to target proteins at or near membranes.
-
-	The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein.,Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E J Mol Biol. 2002 Mar 29;317(3):447-58. PMID:11922676<ref>PMID:11922676</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1gjy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chinese hamster~~]]	+	[[Category: Cricetulus griseus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chiancone, E]]	+	[[Category: Chiancone E]]
-	[[Category: Ilari, A]]	+	[[Category: Ilari A]]
-	[[Category: Johnson~~, K A~~]]	+	[[Category: Johnson KA]]
-	[[Category: Nastopoulos, V]]	+	[[Category: Nastopoulos V]]
-	[[Category: Tsernoglou, D]]	+	[[Category: Tsernoglou D]]
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Calcium-binding]]~~	+
-	~~[[Category: Phosphorylation~~]]	+

OCA at 11:24, 28 July 2021

OCA — Wed, 28 Jul 2021 11:24:56 GMT

←Older revision		Revision as of 11:24, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1gjy' size='340' side='right'caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1gjy' size='340' side='right'caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chinese_hamster Chinese hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GJY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chinese_hamster Chinese hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [~~http~~://pdbe.org/1gjy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gjy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [https://pdbe.org/1gjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [https://www.ebi.ac.uk/pdbsum/1gjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjy ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:02, 23 October 2019

OCA — Wed, 23 Oct 2019 09:02:00 GMT

←Older revision		Revision as of 09:02, 23 October 2019
Line 1:		Line 1:

	==The X-ray structure of the Sorcin Calcium Binding Domain (SCBD) provides insight into the phosphorylation and calcium dependent processess==		==The X-ray structure of the Sorcin Calcium Binding Domain (SCBD) provides insight into the phosphorylation and calcium dependent processess==
-	<StructureSection load='1gjy' size='340' side='right' caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1gjy' size='340' side='right'caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_hamster Chinese hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_hamster Chinese hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Chinese hamster]]		[[Category: Chinese hamster]]
		+	[[Category: Large Structures]]
	[[Category: Chiancone, E]]		[[Category: Chiancone, E]]
	[[Category: Ilari, A]]		[[Category: Ilari, A]]

OCA at 06:41, 3 January 2018

OCA — Wed, 03 Jan 2018 06:41:31 GMT

←Older revision		Revision as of 06:41, 3 January 2018
Line 1:		Line 1:
-	==~~THE~~ X-~~RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN~~ (SCBD) ~~PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS~~==	+
		+	==The X-ray structure of the Sorcin Calcium Binding Domain (SCBD) provides insight into the phosphorylation and calcium dependent processess==
	<StructureSection load='1gjy' size='340' side='right' caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1gjy' size='340' side='right' caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cricetulus_griseus Cricetulus griseus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chinese_hamster Chinese hamster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://pdbe.org/1gjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://pdbe.org/1gjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gjy ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/1gjy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/1gjy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cricetulus griseus~~]]	+	[[Category: Chinese hamster]]
	[[Category: Chiancone, E]]		[[Category: Chiancone, E]]
	[[Category: Ilari, A]]		[[Category: Ilari, A]]

OCA at 18:52, 8 February 2016

OCA — Mon, 08 Feb 2016 18:52:25 GMT

←Older revision		Revision as of 18:52, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gjy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:25, 10 September 2015

OCA — Thu, 10 Sep 2015 14:25:46 GMT

←Older revision		Revision as of 14:25, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://pdbe.org/1gjy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gjy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:06, 22 December 2014

OCA — Mon, 22 Dec 2014 23:06:12 GMT

←Older revision		Revision as of 23:06, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Cricetulus griseus]]		[[Category: Cricetulus griseus]]
-	[[Category: Chiancone, E.]]	+	[[Category: Chiancone, E]]
-	[[Category: Ilari, A.]]	+	[[Category: Ilari, A]]
-	[[Category: Johnson, K A.]]	+	[[Category: Johnson, K A]]
-	[[Category: Nastopoulos, V.]]	+	[[Category: Nastopoulos, V]]
-	[[Category: Tsernoglou, D.]]	+	[[Category: Tsernoglou, D]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
	[[Category: Calcium-binding]]		[[Category: Calcium-binding]]
	[[Category: Phosphorylation]]		[[Category: Phosphorylation]]

OCA at 16:36, 29 September 2014

OCA — Mon, 29 Sep 2014 16:36:49 GMT

←Older revision		Revision as of 16:36, 29 September 2014
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-	[[~~Image~~:1gjy.~~png\|left~~\|200px]]	+	==THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS==
		+	<StructureSection load='1gjy' size='340' side='right' caption='[[1gjy]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gjy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GJY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gjy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gjy RCSB], [http://www.ebi.ac.uk/pdbsum/1gjy PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/1gjy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sorcin is a 21.6 kDa calcium binding protein, expressed in a number of mammalian tissues that belongs to the small, recently identified penta-EF-hand (PEF) family. Like all members of this family, sorcin undergoes a Ca2+-dependent translocation from cytosol to membranes where it binds to target proteins. For sorcin, the targets differ in different tissues, indicating that it takes part in a number of Ca2+-regulated processes. The sorcin monomer is organized in two domains like in all PEF proteins: a flexible, hydrophobic, glycine-rich N-terminal region and a calcium binding C-terminal domain. In vitro, the PEF proteins are dimeric in their Ca2+-free form, but have a marked tendency to precipitate when bound to calcium. Stabilization of the dimeric structure is achieved by pairing of the uneven EF-hand, EF5. Sorcin can also form tetramers at acid pH.The sorcin calcium binding domain (SCBD, residues 33-198) expressed in Escherichia coli was crystallized in the Ca2+-free form. The structure was solved by molecular replacement and was refined to 2.2 A with a crystallographic R-factor of 22.4 %. Interestingly, the asymmetric unit contains two dimers.The structure of the SCBD leads to a model that explains the solution properties and describes the Ca2+-induced conformational changes. Phosphorylation studies show that the N-terminal domain hinders phosphorylation of SCBD, i.e. the rate of phosphorylation increased twofold in the absence of the N-terminal region. In addition, previous fluorescence studies indicated that hydrophobic residues are exposed to solvent upon Ca2+ binding to full-length sorcin. The model accounts for these data by proposing that Ca2+ binding weakens the interactions between the two domains and leads to their reorientation, which exposes hydrophobic regions facilitating the Ca2+-dependent binding to target proteins at or near membranes.

-	~~{{STRUCTURE_1gjy\| PDB=1gjy \| SCENE= }}~~	+	The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein.,Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E J Mol Biol. 2002 Mar 29;317(3):447-58. PMID:11922676<ref>PMID:11922676</ref>

-	~~===THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11922676}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1gjy]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cricetulus_griseus Cricetulus griseus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011922676<~~/~~ref~~><~~ref group="xtra">PMID:011375509</ref><references group="xtra"~~/>	+
	[[Category: Cricetulus griseus]]		[[Category: Cricetulus griseus]]
	[[Category: Chiancone, E.]]		[[Category: Chiancone, E.]]

OCA at 09:36, 16 January 2013

OCA — Wed, 16 Jan 2013 09:36:14 GMT

←Older revision		Revision as of 09:36, 16 January 2013
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	[[Image:1gjy.png\|left\|200px]]		[[Image:1gjy.png\|left\|200px]]

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	{{STRUCTURE_1gjy\| PDB=1gjy \| SCENE= }}		{{STRUCTURE_1gjy\| PDB=1gjy \| SCENE= }}

	===THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS===		===THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11922676}}, adds the Publication Abstract to the page
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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11922676~~</ref><ref group="xtra">PMID:~~11375509~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011922676</ref><ref group="xtra">PMID:011375509</ref><references group="xtra"/>
	[[Category: Cricetulus griseus]]		[[Category: Cricetulus griseus]]
	[[Category: Chiancone, E.]]		[[Category: Chiancone, E.]]