1gkj - Revision history

OCA at 07:51, 15 November 2023

OCA — Wed, 15 Nov 2023 07:51:36 GMT

←Older revision		Revision as of 07:51, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='1gkj' size='340' side='right'caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1gkj' size='340' side='right'caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b8f\|1b8f]]~~, ~~[[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]~~<~~/div></td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [https://pdbe.org/1gkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [https://www.ebi.ac.uk/pdbsum/1gkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [https://pdbe.org/1gkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [https://www.ebi.ac.uk/pdbsum/1gkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HUTH_PSEPU HUTH_PSEPU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens putidus flugge 1886]]
-	[[Category: Histidine ammonia-lyase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Baedeker, M~~]]	+	[[Category: Pseudomonas putida]]
-	[[Category: ~~Schulz, G E~~]]	+	[[Category: Baedeker M]]
-	[[Category: ~~Histidine degradation]]~~	+	[[Category: Schulz GE]]
-	~~[[Category: Lyase~~]]	+

OCA at 11:25, 28 July 2021

OCA — Wed, 28 Jul 2021 11:25:07 GMT

←Older revision		Revision as of 11:25, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1gkj' size='340' side='right'caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1gkj' size='340' side='right'caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [~~http~~://pdbe.org/1gkj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gkj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [https://pdbe.org/1gkj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [https://www.ebi.ac.uk/pdbsum/1gkj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:07, 23 October 2019

OCA — Wed, 23 Oct 2019 07:07:31 GMT

←Older revision		Revision as of 07:07, 23 October 2019
Line 1:		Line 1:

	==Histidine Ammonia-Lyase (HAL) Mutant Y280F from Pseudomonas putida==		==Histidine Ammonia-Lyase (HAL) Mutant Y280F from Pseudomonas putida==
-	<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1gkj' size='340' side='right'caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
Line 35:		Line 35:
	[[Category: Bacillus fluorescens putidus flugge 1886]]		[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Histidine ammonia-lyase]]		[[Category: Histidine ammonia-lyase]]
		+	[[Category: Large Structures]]
	[[Category: Baedeker, M]]		[[Category: Baedeker, M]]
	[[Category: Schulz, G E]]		[[Category: Schulz, G E]]
	[[Category: Histidine degradation]]		[[Category: Histidine degradation]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 05:58, 20 June 2018

OCA — Wed, 20 Jun 2018 05:58:01 GMT

←Older revision		Revision as of 05:58, 20 June 2018
Line 1:		Line 1:

-	==~~HISTIDINE AMMONIA~~-~~LYASE~~ (HAL) ~~MUTANT~~ Y280F ~~FROM PSEUDOMONAS PUTIDA~~==	+	==Histidine Ammonia-Lyase (HAL) Mutant Y280F from Pseudomonas putida==
	<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 06:37, 3 January 2018

OCA — Wed, 03 Jan 2018 06:37:21 GMT

←Older revision		Revision as of 06:37, 3 January 2018
Line 1:		Line 1:
		+
	==HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA==		==HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA==
	<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://pdbe.org/1gkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://pdbe.org/1gkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gkj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gkj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gkj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:01, 8 February 2016

OCA — Mon, 08 Feb 2016 17:01:41 GMT

←Older revision		Revision as of 17:01, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gkj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:38, 10 September 2015

OCA — Thu, 10 Sep 2015 13:38:12 GMT

←Older revision		Revision as of 13:38, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_putida Pseudomonas putida~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://pdbe.org/1gkj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gkj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens putidus flugge 1886]]
	[[Category: Histidine ammonia-lyase]]		[[Category: Histidine ammonia-lyase]]
-	[[Category: Pseudomonas putida]]
	[[Category: Baedeker, M]]		[[Category: Baedeker, M]]
	[[Category: Schulz, G E]]		[[Category: Schulz, G E]]
	[[Category: Histidine degradation]]		[[Category: Histidine degradation]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 22:10, 22 December 2014

OCA — Mon, 22 Dec 2014 22:10:58 GMT

←Older revision		Revision as of 22:10, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Histidine ammonia-lyase]]		[[Category: Histidine ammonia-lyase]]
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]
-	[[Category: Baedeker, M.]]	+	[[Category: Baedeker, M]]
-	[[Category: Schulz, G E.]]	+	[[Category: Schulz, G E]]
	[[Category: Histidine degradation]]		[[Category: Histidine degradation]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 10:25, 3 October 2014

OCA — Fri, 03 Oct 2014 10:25:13 GMT

←Older revision		Revision as of 10:25, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1gkj\| PDB=1gkj \| SCENE= }}~~	+	==HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA==
-	===HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA===	+	<StructureSection load='1gkj' size='340' side='right' caption='[[1gkj]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	{~~{ABSTRACT_PUBMED_11895450}~~}	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gkj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GKJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b8f\|1b8f]], [[1eb4\|1eb4]], [[1gk2\|1gk2]], [[1gk3\|1gk3]], [[1gkm\|1gkm]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_ammonia-lyase Histidine ammonia-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.1.3 4.3.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gkj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gkj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gkj RCSB], [http://www.ebi.ac.uk/pdbsum/1gkj PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gkj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Histidine ammonia-lyase (EC 4.3.1.3) catalyzes the nonoxidative elimination of the alpha-amino group of histidine using a 4-methylidene-imidazole-5-one (MIO), which is formed autocatalytically from the internal peptide segment 142Ala-Ser-Gly. The structure of the enzyme inhibited by a reaction with l-cysteine was established at the very high resolution of 1.0 A. Five active center mutants were produced and their catalytic activities were measured. Among them, mutant Tyr280-->Phe could be crystallized and its structure could be determined at 1.7 A resolution. It contains a planar sp2-hybridized 144-N atom of MIO, in contrast to the pyramidal sp3-hybridized 144-N of the wild-type. With the planar 144-N atom, MIO assumes the conformation of a putative intermediate aromatic state of the reaction, demonstrating that the conformational barrier between aromatic and wild-type states is very low. The data led to a new proposal for the geometry for the catalyzed reaction, which also applies to the closely related phenylalanine ammonia-lyase (EC 4.3.1.5). Moreover, it suggested an intermediate binding site for the released ammonia.

-	~~==About this Structure==~~	+	Structures of two histidine ammonia-lyase modifications and implications for the catalytic mechanism.,Baedeker M, Schulz GE Eur J Biochem. 2002 Mar;269(6):1790-7. PMID:11895450<ref>PMID:11895450</ref>
-	~~[[1gkj]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_putida Pseudomonas putida]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GKJ OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:011895450</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Histidine ammonia-lyase]]		[[Category: Histidine ammonia-lyase]]
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]

OCA at 10:35, 16 April 2014

OCA — Wed, 16 Apr 2014 10:35:28 GMT

←Older revision		Revision as of 10:35, 16 April 2014
Line 1:		Line 1:
-	[[Image:1gkj.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_1gkj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1gkj\| PDB=1gkj \| SCENE= }}		{{STRUCTURE_1gkj\| PDB=1gkj \| SCENE= }}
-
	===HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA===		===HISTIDINE AMMONIA-LYASE (HAL) MUTANT Y280F FROM PSEUDOMONAS PUTIDA===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11895450}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11895450 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11895450}}		{{ABSTRACT_PUBMED_11895450}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:011895450</ref><~~ref~~ group="xtra"~~>PMID:010220322<~~/~~ref~~><references ~~group="xtra"~~/>	+	<ref group="xtra">PMID:011895450</ref><references group="xtra"/><references/>
	[[Category: Histidine ammonia-lyase]]		[[Category: Histidine ammonia-lyase]]
	[[Category: Pseudomonas putida]]		[[Category: Pseudomonas putida]]