1gns - Revision history

OCA at 12:01, 13 December 2023

OCA — Wed, 13 Dec 2023 12:01:04 GMT

←Older revision		Revision as of 12:01, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1gns' size='340' side='right'caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gns' size='340' side='right'caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1GNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GNS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACN:ACETONE'>ACN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CSO~~:~~S-HYDROXYCYSTEINE~~'>~~CSO~~</scene>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]]~~, [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [https://pdbe.org/1gns PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [https://www.ebi.ac.uk/pdbsum/1gns PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gns ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [~~http~~://pdbe.org/1gns PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gns PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gns ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>	+	[https://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 38:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Subtilisin]]~~	+	[[Category: Alexander P]]
-	[[Category: Alexander, P]]	+	[[Category: Almog O]]
-	[[Category: Almog, O]]	+	[[Category: Gallagher DT]]
-	[[Category: Gallagher~~, D T~~]]	+	[[Category: Ladner JE]]
-	[[Category: Ladner~~, J E~~]]	+	[[Category: Strausberg S]]
-	[[Category: Strausberg, S]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine proteinase~~]]	+

OCA at 11:48, 29 July 2020

OCA — Wed, 29 Jul 2020 11:48:01 GMT

←Older revision		Revision as of 11:48, 29 July 2020
Line 1:		Line 1:

	==SUBTILISIN BPN'==		==SUBTILISIN BPN'==
-	<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1gns' size='340' side='right'caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1GNS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://pdbe.org/1gns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gns ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://pdbe.org/1gns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gns ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Subtilisin\|Subtilisin]]	+	*[[Subtilisin 3D structures\|Subtilisin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Alexander, P]]		[[Category: Alexander, P]]

OCA at 07:57, 24 October 2018

OCA — Wed, 24 Oct 2018 07:57:50 GMT

←Older revision		Revision as of 07:57, 24 October 2018
Line 16:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gns_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gns_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:20, 18 January 2017

OCA — Wed, 18 Jan 2017 17:20:07 GMT

←Older revision		Revision as of 17:20, 18 January 2017
Line 1:		Line 1:
		+
	==SUBTILISIN BPN'==		==SUBTILISIN BPN'==
	<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://pdbe.org/1gns PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gns ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gns ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gns" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:31, 15 April 2015

OCA — Wed, 15 Apr 2015 06:31:02 GMT

←Older revision		Revision as of 06:31, 15 April 2015
Line 2:		Line 2:
	<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_amyloliquefaciens Bacillus amyloliquefaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GNS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GNS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus amyloliquefaciens]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
		+	[[Category: Alexander, P]]
	[[Category: Almog, O]]		[[Category: Almog, O]]
-	[[Category: Bryan, P Alexanderp]]
	[[Category: Gallagher, D T]]		[[Category: Gallagher, D T]]
-	[[Category: Gilliland, G L]]
	[[Category: Ladner, J E]]		[[Category: Ladner, J E]]
	[[Category: Strausberg, S]]		[[Category: Strausberg, S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine proteinase]]		[[Category: Serine proteinase]]

OCA at 19:17, 25 December 2014

OCA — Thu, 25 Dec 2014 19:17:29 GMT

←Older revision		Revision as of 19:17, 25 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUBT_BACAM SUBT_BACAM]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin.<ref>PMID:12524032</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:46, 22 December 2014

OCA — Mon, 22 Dec 2014 18:46:21 GMT

←Older revision		Revision as of 18:46, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GNS FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GNS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
-	[[Category: Almog, O.]]	+	[[Category: Almog, O]]
-	[[Category: Bryan, P Alexanderp.]]	+	[[Category: Bryan, P Alexanderp]]
-	[[Category: Gallagher, D T.]]	+	[[Category: Gallagher, D T]]
-	[[Category: Gilliland, G L.]]	+	[[Category: Gilliland, G L]]
-	[[Category: Ladner, J E.]]	+	[[Category: Ladner, J E]]
-	[[Category: Strausberg, S.]]	+	[[Category: Strausberg, S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine proteinase]]		[[Category: Serine proteinase]]

OCA at 08:47, 28 September 2014

OCA — Sun, 28 Sep 2014 08:47:40 GMT

←Older revision		Revision as of 08:47, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1gns.~~png~~\|~~left~~\|200px]]	+	==SUBTILISIN BPN'==
		+	<StructureSection load='1gns' size='340' side='right' caption='[[1gns]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GNS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACN:ACETONE'>ACN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1a2q\|1a2q]], [[1ak9\|1ak9]], [[1aqn\|1aqn]], [[1au9\|1au9]], [[1gnv\|1gnv]], [[1s01\|1s01]], [[1s02\|1s02]], [[1sbh\|1sbh]], [[1sbi\|1sbi]], [[1sbn\|1sbn]], [[1sbt\|1sbt]], [[1sib\|1sib]], [[1spb\|1spb]], [[1st2\|1st2]], [[1sua\|1sua]], [[1sub\|1sub]], [[1suc\|1suc]], [[1sud\|1sud]], [[1sue\|1sue]], [[1sup\|1sup]], [[1ubn\|1ubn]], [[1yja\|1yja]], [[1yjb\|1yjb]], [[1yjc\|1yjc]], [[2sbt\|2sbt]], [[2sic\|2sic]], [[2sni\|2sni]], [[2st1\|2st1]], [[3sic\|3sic]], [[5sic\|5sic]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gns OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gns RCSB], [http://www.ebi.ac.uk/pdbsum/1gns PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gns_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of two thermally stabilized subtilisin BPN' variants, S63 and S88, are reported here at 1.8 and 1.9 A resolution, respectively. The micromolar affinity calcium binding site (site A) has been deleted (Delta75-83) in these variants, enabling the activity and thermostability measurements in chelating conditions. Each of the variants includes mutations known previously to increase the thermostability of calcium-independent subtilisin in addition to new stabilizing mutations. S63 has eight amino acid replacements: D41A, M50F, A73L, Q206W, Y217K, N218S, S221C, and Q271E. S63 has 75-fold greater stability than wild type subtilisin in chelating conditions (10 mm EDTA). The other variant, S88, has ten site-specific changes: Q2K, S3C, P5S, K43N, M50F, A73L, Q206C, Y217K, N218S, and Q271E. The two new cysteines form a disulfide bond, and S88 has 1000 times greater stability than wild type subtilisin in chelating conditions. Comparisons of the two new crystal structures (S63 in space group P2(1) with A cell constants 41.2, 78.1, 36.7, and beta = 114.6 degrees and S88 in space group P2(1)2(1)2(1) with cell constants 54.2, 60.4, and 82.7) with previous structures of subtilisin BPN' reveal that the principal changes are in the N-terminal region. The structural bases of the stabilization effects of the new mutations Q2K, S3C, P5S, D41A, Q206C, and Q206W are generally apparent. The effects are attributed to the new disulfide cross-link and to improved hydrophobic packing, new hydrogen bonds, and other rearrangements in the N-terminal region.

-	~~{{STRUCTURE_1gns\| PDB=1gns \| SCENE= }}~~	+	Structural basis of thermostability. Analysis of stabilizing mutations in subtilisin BPN'.,Almog O, Gallagher DT, Ladner JE, Strausberg S, Alexander P, Bryan P, Gilliland GL J Biol Chem. 2002 Jul 26;277(30):27553-8. Epub 2002 May 13. PMID:12011071<ref>PMID:12011071</ref>

-	~~===SUBTILISIN BPN'===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12011071}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1gns]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA].~~	+

	==See Also==		==See Also==
	*[[Subtilisin\|Subtilisin]]		*[[Subtilisin\|Subtilisin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012011071<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]

OCA at 20:27, 20 October 2012

OCA — Sat, 20 Oct 2012 20:27:28 GMT

←Older revision		Revision as of 20:27, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1gns]] is a 1 chain structure ~~of [[Subtilisin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA].	+	[[1gns]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNS OCA].

	==See Also==		==See Also==

OCA at 10:32, 27 July 2012

OCA — Fri, 27 Jul 2012 10:32:42 GMT

←Older revision		Revision as of 10:32, 27 July 2012
Line 1:		Line 1:
	[[Image:1gns.png\|left\|200px]]		[[Image:1gns.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1gns", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1gns\| PDB=1gns \| SCENE= }}		{{STRUCTURE_1gns\| PDB=1gns \| SCENE= }}

	===SUBTILISIN BPN'===		===SUBTILISIN BPN'===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12011071}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12011071 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12011071}}		{{ABSTRACT_PUBMED_12011071}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Subtilisin]]	+	*[[Subtilisin\|Subtilisin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12011071~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012011071</ref><references group="xtra"/>
	[[Category: Bacillus amyloliquefaciens]]		[[Category: Bacillus amyloliquefaciens]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]