1gr7 - Revision history

OCA at 11:24, 27 March 2024

OCA — Wed, 27 Mar 2024 11:24:06 GMT

←Older revision		Revision as of 11:24, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [https://pdbe.org/1gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [https://pdbe.org/1gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.	+	[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gr7 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gr7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Azurin is a cupredoxin, which functions as an electron carrier. Its fold is dominated by a beta-sheet structure. In the present study, azurin serves as a model system to investigate the importance of a conserved disulphide bond for protein stability and folding/unfolding. For this purpose, we have examined two azurin mutants, the single mutant Cys3Ser, which disrupts azurin's conserved disulphide bond, and the double mutant Cys3Ser/Ser100Pro, which contains an additional mutation at a site distant from the conserved disulphide. The crystal structure of the azurin double mutant has been determined to 1.8 A resolution(2), with a crystallographic R-factor of 17.5% (R(free)=20.8%). A comparison with the wild-type structure reveals that structural differences are limited to the sites of the mutations. Also, the rates of folding and unfolding as determined by CD and fluorescence spectroscopy are almost unchanged. The main difference to wild-type azurin is a destabilisation by approximately 20 kJ x mol(-1), constituting half the total folding energy of the wild-type protein. Thus, the disulphide bond constitutes a vital component in giving azurin its stable fold.
-
-	Crystal structure of the double azurin mutant Cys3Ser/Ser100Pro from Pseudomonas aeruginosa at 1.8 A resolution: its folding-unfolding energy and unfolding kinetics.,Okvist M, Bonander N, Sandberg A, Karlsson BG, Krengel U, Xue Y, Sjolin L Biochim Biophys Acta. 2002 Apr 29;1596(2):336-45. PMID:12007613<ref>PMID:12007613</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Azurin 3D structures\|Azurin 3D structures]]		*[[Azurin 3D structures\|Azurin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bonander, N]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Karlsson~~, B G~~]]	+	[[Category: Bonander N]]
-	[[Category: Krengel, U]]	+	[[Category: Karlsson BG]]
-	[[Category: Okvist, M]]	+	[[Category: Krengel U]]
-	[[Category: Sandberg, A]]	+	[[Category: Okvist M]]
-	[[Category: Sjolin, L]]	+	[[Category: Sandberg A]]
-	[[Category: Xue, Y]]	+	[[Category: Sjolin L]]
-	~~[[Category: Electron transport~~]]	+	[[Category: Xue Y]]

OCA at 10:47, 14 July 2021

OCA — Wed, 14 Jul 2021 10:47:27 GMT

←Older revision		Revision as of 10:47, 14 July 2021
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	<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [~~http~~://pdbe.org/1gr7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [https://pdbe.org/1gr7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.	+	[[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 05:31, 10 October 2019

OCA — Thu, 10 Oct 2019 05:31:17 GMT

←Older revision		Revision as of 05:31, 10 October 2019
Line 1:		Line 1:

	==Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution==		==Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution==
-	<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1gr7' size='340' side='right'caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Azurin 3D structures\|Azurin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bonander, N]]		[[Category: Bonander, N]]
	[[Category: Karlsson, B G]]		[[Category: Karlsson, B G]]

OCA at 06:30, 3 January 2018

OCA — Wed, 03 Jan 2018 06:30:50 GMT

←Older revision		Revision as of 06:30, 3 January 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE DOUBLE MUTANT CYS3SER~~/~~SER100PRO FROM PSEUDOMONAS AERUGINOSA AT~~ 1.8 A ~~RESOLUTION~~==	+
		+	==Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution==
	<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://pdbe.org/1gr7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://pdbe.org/1gr7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1gr7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1gr7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Azurin\|Azurin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:11, 9 February 2016

OCA — Tue, 09 Feb 2016 22:11:51 GMT

←Older revision		Revision as of 22:11, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gr7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:31, 11 September 2015

OCA — Fri, 11 Sep 2015 02:31:44 GMT

←Older revision		Revision as of 02:31, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://pdbe.org/1gr7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gr7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Bonander, N]]		[[Category: Bonander, N]]
	[[Category: Karlsson, B G]]		[[Category: Karlsson, B G]]

OCA at 09:52, 25 December 2014

OCA — Thu, 25 Dec 2014 09:52:57 GMT

←Older revision		Revision as of 09:52, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 22:52, 22 December 2014

OCA — Mon, 22 Dec 2014 22:52:24 GMT

←Older revision		Revision as of 22:52, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Bonander, N.]]	+	[[Category: Bonander, N]]
-	[[Category: Karlsson, B G.]]	+	[[Category: Karlsson, B G]]
-	[[Category: Krengel, U.]]	+	[[Category: Krengel, U]]
-	[[Category: Okvist, M.]]	+	[[Category: Okvist, M]]
-	[[Category: Sandberg, A.]]	+	[[Category: Sandberg, A]]
-	[[Category: Sjolin, L.]]	+	[[Category: Sjolin, L]]
-	[[Category: Xue, Y.]]	+	[[Category: Xue, Y]]
	[[Category: Electron transport]]		[[Category: Electron transport]]

OCA at 12:38, 28 September 2014

OCA — Sun, 28 Sep 2014 12:38:18 GMT

←Older revision		Revision as of 12:38, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1gr7.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE DOUBLE MUTANT CYS3SER/SER100PRO FROM PSEUDOMONAS AERUGINOSA AT 1.8 A RESOLUTION==
		+	<StructureSection load='1gr7' size='340' side='right' caption='[[1gr7]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gr7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GR7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ag0\|1ag0]], [[1azn\|1azn]], [[1azr\|1azr]], [[1azu\|1azu]], [[1bex\|1bex]], [[1cc3\|1cc3]], [[1e5y\|1e5y]], [[1e5z\|1e5z]], [[1e65\|1e65]], [[1e67\|1e67]], [[1etj\|1etj]], [[1ezl\|1ezl]], [[1i53\|1i53]], [[1ils\|1ils]], [[1ilu\|1ilu]], [[1jze\|1jze]], [[1jzf\|1jzf]], [[1jzg\|1jzg]], [[1jzh\|1jzh]], [[1jzi\|1jzi]], [[1jzj\|1jzj]], [[1nzr\|1nzr]], [[1vlx\|1vlx]], [[2azu\|2azu]], [[2tsa\|2tsa]], [[2tsb\|2tsb]], [[3azu\|3azu]], [[4azu\|4azu]], [[5azu\|5azu]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gr7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gr7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gr7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1gr7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Azurin is a cupredoxin, which functions as an electron carrier. Its fold is dominated by a beta-sheet structure. In the present study, azurin serves as a model system to investigate the importance of a conserved disulphide bond for protein stability and folding/unfolding. For this purpose, we have examined two azurin mutants, the single mutant Cys3Ser, which disrupts azurin's conserved disulphide bond, and the double mutant Cys3Ser/Ser100Pro, which contains an additional mutation at a site distant from the conserved disulphide. The crystal structure of the azurin double mutant has been determined to 1.8 A resolution(2), with a crystallographic R-factor of 17.5% (R(free)=20.8%). A comparison with the wild-type structure reveals that structural differences are limited to the sites of the mutations. Also, the rates of folding and unfolding as determined by CD and fluorescence spectroscopy are almost unchanged. The main difference to wild-type azurin is a destabilisation by approximately 20 kJ x mol(-1), constituting half the total folding energy of the wild-type protein. Thus, the disulphide bond constitutes a vital component in giving azurin its stable fold.

-	~~{{STRUCTURE_1gr7\| PDB=1gr7 \| SCENE= }}~~	+	Crystal structure of the double azurin mutant Cys3Ser/Ser100Pro from Pseudomonas aeruginosa at 1.8 A resolution: its folding-unfolding energy and unfolding kinetics.,Okvist M, Bonander N, Sandberg A, Karlsson BG, Krengel U, Xue Y, Sjolin L Biochim Biophys Acta. 2002 Apr 29;1596(2):336-45. PMID:12007613<ref>PMID:12007613</ref>

-	~~===CRYSTAL STRUCTURE OF THE DOUBLE MUTANT CYS3SER~~/~~SER100PRO FROM PSEUDOMONAS AERUGINOSA AT 1~~.~~8 A RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12007613}}~~	+	==See Also==
-		+	*[[Azurin\|Azurin]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1gr7~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR7 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012007613<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Bonander, N.]]		[[Category: Bonander, N.]]

OCA: Protected "1gr7" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:52:37 GMT

Protected "1gr7" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:52, 21 November 2012