1gs7 - Revision history

OCA at 12:05, 13 December 2023

OCA — Wed, 13 Dec 2023 12:05:10 GMT

←Older revision		Revision as of 12:05, 13 December 2023
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	<StructureSection load='1gs7' size='340' side='right'caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1gs7' size='340' side='right'caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter~~ xylosoxidans~~" yabuuchi and ohyama 1971~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_xylosoxidans Achromobacter xylosoxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1gs6\|1gs6]]~~, ~~[[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [https://pdbe.org/1gs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gs7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [https://pdbe.org/1gs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gs7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O68601_ALCXX O68601_ALCXX]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Achromobacter xylosoxidans ~~yabuuchi and ohyama 1971~~]]	+	[[Category: Achromobacter xylosoxidans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dodd~~, F E~~]]	+	[[Category: Dodd FE]]
-	[[Category: Eady~~, R R~~]]	+	[[Category: Eady RR]]
-	[[Category: Ellis~~, M J~~]]	+	[[Category: Ellis MJ]]
-	[[Category: Hasnain~~, S S~~]]	+	[[Category: Hasnain SS]]
-	[[Category: Prudencio, M]]	+	[[Category: Prudencio M]]
-	[[Category: Sawers, G]]	+	[[Category: Sawers G]]
-	[[Category: Strange~~, R W]]~~	+	[[Category: Strange RW]]
-	~~[[Category: Multicopper]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Periplasmic~~]]	+

OCA at 11:27, 28 July 2021

OCA — Wed, 28 Jul 2021 11:27:52 GMT

←Older revision		Revision as of 11:27, 28 July 2021
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	<StructureSection load='1gs7' size='340' side='right'caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1gs7' size='340' side='right'caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [~~http~~://pdbe.org/1gs7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gs7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gs7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [https://pdbe.org/1gs7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gs7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gs7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Nitrite reductase\|Nitrite reductase]]	+	*[[Nitrite reductase 3D structures\|Nitrite reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:23, 23 October 2019

OCA — Wed, 23 Oct 2019 08:23:55 GMT

←Older revision		Revision as of 08:23, 23 October 2019
Line 1:		Line 1:

	==Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase==		==Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase==
-	<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='1gs7' size='340' side='right'caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1gs7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gs7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitrite reductase\|Nitrite reductase]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Achromobacter xylosoxidans yabuuchi and ohyama 1971]]		[[Category: Achromobacter xylosoxidans yabuuchi and ohyama 1971]]
		+	[[Category: Large Structures]]
	[[Category: Dodd, F E]]		[[Category: Dodd, F E]]
	[[Category: Eady, R R]]		[[Category: Eady, R R]]

OCA at 06:13, 3 January 2018

OCA — Wed, 03 Jan 2018 06:13:35 GMT

←Older revision		Revision as of 06:13, 3 January 2018
Line 1:		Line 1:
		+
	==Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase==		==Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase==
	<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://pdbe.org/1gs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://pdbe.org/1gs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gs7 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gs7_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gs7_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1gs7" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1gs7" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Nitric reductase\|Nitric reductase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:58, 7 February 2016

OCA — Sun, 07 Feb 2016 23:58:00 GMT

←Older revision		Revision as of 23:58, 7 February 2016
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://pdbe.org/1gs7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gs7 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gs7" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:18, 15 April 2015

OCA — Wed, 15 Apr 2015 06:18:07 GMT

←Older revision		Revision as of 06:18, 15 April 2015
Line 2:		Line 2:
	<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermomicrobium_roseum Thermomicrobium roseum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermomicrobium roseum~~]]	+	[[Category: Achromobacter xylosoxidans yabuuchi and ohyama 1971]]
	[[Category: Dodd, F E]]		[[Category: Dodd, F E]]
	[[Category: Eady, R R]]		[[Category: Eady, R R]]

OCA at 20:54, 22 December 2014

OCA — Mon, 22 Dec 2014 20:54:30 GMT

←Older revision		Revision as of 20:54, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Thermomicrobium roseum]]		[[Category: Thermomicrobium roseum]]
-	[[Category: Dodd, F E.]]	+	[[Category: Dodd, F E]]
-	[[Category: Eady, R R.]]	+	[[Category: Eady, R R]]
-	[[Category: Ellis, M J.]]	+	[[Category: Ellis, M J]]
-	[[Category: Hasnain, S S.]]	+	[[Category: Hasnain, S S]]
-	[[Category: Prudencio, M.]]	+	[[Category: Prudencio, M]]
-	[[Category: Sawers, G.]]	+	[[Category: Sawers, G]]
-	[[Category: Strange, R W.]]	+	[[Category: Strange, R W]]
	[[Category: Multicopper]]		[[Category: Multicopper]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Periplasmic]]		[[Category: Periplasmic]]

OCA at 10:45, 28 September 2014

OCA — Sun, 28 Sep 2014 10:45:59 GMT

←Older revision		Revision as of 10:45, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1gs7.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase==
		+	<StructureSection load='1gs7' size='340' side='right' caption='[[1gs7]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GS7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gs6\|1gs6]], [[1gs8\|1gs8]], [[1hau\|1hau]], [[1haw\|1haw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gs7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gs7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gs7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gs7 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/1gs7_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dissimilatory nitrite reductase catalyses the reduction of nitrite (NO(2)(-)) to nitric oxide (NO). Copper-containing nitrite reductases contain both type 1 and type 2 Cu sites. Electron transfer from redox partners is presumed to be mediated via the type 1 Cu site and used at the catalytic type 2 Cu centre along with the substrate nitrite. At the type 2 Cu site, Asp92 has been identified as a key residue in substrate utilisation, since it hydrogen bonds to the water molecule at the nitrite binding site. We have also suggested that protons enter the catalytic site via Asp92, through a water network that is mediated by His254. The role of these residues has been investigated in the blue copper nitrite reductase from Alcaligenes xylosoxidans (NCIMB 11015) by a combination of point mutation, enzymatic activity measurement and structure determination.In addition, it has been suggested that the enzyme operates via an ordered mechanism where an electron is transferred to the type 2 Cu site largely when the second substrate nitrite is bound and that this is controlled via the lowering of the redox potential of the type 2 site when it is loaded with nitrite. Thus, a small perturbation of the type 1 Cu site should result in a significant effect on the activity of the enzyme. For this reason a mutation of Met144, which is the weakest ligand of the type 1 Cu, is investigated. The structures of H254F, D92N and M144A have been determined to 1.85 A, 1.9 A and 2.2 A resolution, respectively. The D92N and H254F mutants have negligible or no activity, while the M144A mutant has 30 % activity of the native enzyme. Structural and spectroscopic data show that the loss of activity in H254F is due to the catalytic site being occupied by Zn while the loss/reduction of activity in D92N/M144A are due to structural reasons. The D92N mutation results in the loss of the Asp92 hydrogen bond to the Cu-ligated water. Therefore, the ligand is no longer able to perform proton abstraction. Even though the loss of activity in H254F is due to lack of catalytic Cu, the mutation does cause the disruption of the water network, confirming its key role in proton channel. The structure of the H254F mutant is the first case where full occupancy Zn at the type 2 Cu site is observed, but despite the previously noted similarity of this site to the carbonic anhydrase catalytic site, no carbonic anhydrase activity is observed. The H254F and D92N mutant structures provide, for the first time, observation of surface Zn sites which may act as a Zn sink and prevent binding of Zn at the catalytic Cu site in the native enzyme.

-	~~{{STRUCTURE_1gs7\| PDB=1gs7 \| SCENE= }}~~	+	Biochemical and crystallographic studies of the Met144Ala, Asp92Asn and His254Phe mutants of the nitrite reductase from Alcaligenes xylosoxidans provide insight into the enzyme mechanism.,Ellis MJ, Prudencio M, Dodd FE, Strange RW, Sawers G, Eady RR, Hasnain SS J Mol Biol. 2002 Feb 8;316(1):51-64. PMID:11829502<ref>PMID:11829502</ref>

-	~~===Crystal structure~~ of ~~H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11829502}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1gs7]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA].~~	+

	==See Also==		==See Also==
	*[[Nitric reductase\|Nitric reductase]]		*[[Nitric reductase\|Nitric reductase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011829502<~~/~~ref~~><~~ref group="xtra">PMID:019053252</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Thermomicrobium roseum]]		[[Category: Thermomicrobium roseum]]
	[[Category: Dodd, F E.]]		[[Category: Dodd, F E.]]

OCA at 08:13, 20 October 2012

OCA — Sat, 20 Oct 2012 08:13:45 GMT

←Older revision		Revision as of 08:13, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1gs7]] is a 1 chain structure ~~of [[Nitric reductase]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA].	+	[[1gs7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GS7 OCA].

	==See Also==		==See Also==

OCA at 18:51, 25 July 2012

OCA — Wed, 25 Jul 2012 18:51:27 GMT

←Older revision		Revision as of 18:51, 25 July 2012
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	[[Image:1gs7.png\|left\|200px]]		[[Image:1gs7.png\|left\|200px]]

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	{{STRUCTURE_1gs7\| PDB=1gs7 \| SCENE= }}		{{STRUCTURE_1gs7\| PDB=1gs7 \| SCENE= }}

	===Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase===		===Crystal structure of H254F mutant of Alcaligenes xylosoxidans Nitrite Reductase===

-
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