1gug - Revision history

OCA at 08:51, 9 May 2024

OCA — Thu, 09 May 2024 08:51:04 GMT

←Older revision		Revision as of 08:51, 9 May 2024
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gug ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gug ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MopII from Clostridium pasteurianum is a molbindin family member. These proteins may serve as intracellular storage facilities for molybdate, which they bind with high specificity. High resolution structures of MopII in a number of states, including the first structure of an apo-molbindin, together with calorimetric data, allow us to describe ligand binding and provide support for the proposed storage function of the protein. MopII assembles as a trimer of dimers and binds eight oxyanions at two types of binding sites located at intersubunit interfaces. Two type 1 sites are on the molecular 3-fold axis and three pairs of type 2 sites occur on the molecular 2-fold axes. The hexamer is largely unaffected by the binding of ligand. Molybdate is admitted to the otherwise inaccessible type 2 binding sites by the movement of the N-terminal residues of each protein chain. This contrasts with the structurally related molybdate-dependent transcriptional regulator ModE, which undergoes extensive conformational rearrangements on ligand binding. Despite similarities between the binding sites of ModE and the type 2 sites of MopII the molbindin has a significantly reduced ligand affinity, due, in part, to the high density of negative charges at the center of the hexamer. In the absence of ligand this effects the movement of an important lysine side chain, thereby partially inactivating the binding sites. The differences are consistent with a biological role in molybdate storage/buffering.
		+
		+	Passive acquisition of ligand by the MopII molbindin from Clostridium pasteurianum: structures of apo and oxyanion-bound forms.,Schuttelkopf AW, Harrison JA, Boxer DH, Hunter WN J Biol Chem. 2002 Apr 26;277(17):15013-20. Epub 2002 Feb 8. PMID:11836258<ref>PMID:11836258</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1gug" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:24, 27 March 2024

OCA — Wed, 27 Mar 2024 11:24:36 GMT

←Older revision		Revision as of 11:24, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1gug' size='340' side='right'caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1gug' size='340' side='right'caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [https://pdbe.org/1gug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [https://www.ebi.ac.uk/pdbsum/1gug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [https://pdbe.org/1gug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [https://www.ebi.ac.uk/pdbsum/1gug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA]] Binds one mole of molybdenum per mole of protein and contains a pterin.	+	[https://www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA] Binds one mole of molybdenum per mole of protein and contains a pterin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gug ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gug ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	MopII from Clostridium pasteurianum is a molbindin family member. These proteins may serve as intracellular storage facilities for molybdate, which they bind with high specificity. High resolution structures of MopII in a number of states, including the first structure of an apo-molbindin, together with calorimetric data, allow us to describe ligand binding and provide support for the proposed storage function of the protein. MopII assembles as a trimer of dimers and binds eight oxyanions at two types of binding sites located at intersubunit interfaces. Two type 1 sites are on the molecular 3-fold axis and three pairs of type 2 sites occur on the molecular 2-fold axes. The hexamer is largely unaffected by the binding of ligand. Molybdate is admitted to the otherwise inaccessible type 2 binding sites by the movement of the N-terminal residues of each protein chain. This contrasts with the structurally related molybdate-dependent transcriptional regulator ModE, which undergoes extensive conformational rearrangements on ligand binding. Despite similarities between the binding sites of ModE and the type 2 sites of MopII the molbindin has a significantly reduced ligand affinity, due, in part, to the high density of negative charges at the center of the hexamer. In the absence of ligand this effects the movement of an important lysine side chain, thereby partially inactivating the binding sites. The differences are consistent with a biological role in molybdate storage/buffering.
-
-	Passive acquisition of ligand by the MopII molbindin from Clostridium pasteurianum: structures of apo and oxyanion-bound forms.,Schuttelkopf AW, Harrison JA, Boxer DH, Hunter WN J Biol Chem. 2002 Apr 26;277(17):15013-20. Epub 2002 Feb 8. PMID:11836258<ref>PMID:11836258</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1gug" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clostridium pasteurianum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harrison~~, J A~~]]	+	[[Category: Harrison JA]]
-	[[Category: Hunter~~, W N~~]]	+	[[Category: Hunter WN]]
-	[[Category: Schuettelkopf~~, A W]]~~	+	[[Category: Schuettelkopf AW]]
-	~~[[Category: Molbindin]]~~	+
-	~~[[Category: Molybdate binding]]~~	+
-	~~[[Category: Molybdate binding protein]]~~	+
-	~~[[Category: Mop~~]]	+

OCA at 11:28, 28 July 2021

OCA — Wed, 28 Jul 2021 11:28:44 GMT

←Older revision		Revision as of 11:28, 28 July 2021
Line 3:		Line 3:
	<StructureSection load='1gug' size='340' side='right'caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1gug' size='340' side='right'caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1GUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [~~http~~://pdbe.org/1gug PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1gug PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1gug ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [https://pdbe.org/1gug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [https://www.ebi.ac.uk/pdbsum/1gug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA]] Binds one mole of molybdenum per mole of protein and contains a pterin.	+	[[https://www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA]] Binds one mole of molybdenum per mole of protein and contains a pterin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:10, 23 October 2019

OCA — Wed, 23 Oct 2019 09:10:39 GMT

←Older revision		Revision as of 09:10, 23 October 2019
Line 1:		Line 1:

	==MopII from Clostridium pasteurianum complexed with tungstate==		==MopII from Clostridium pasteurianum complexed with tungstate==
-	<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='1gug' size='340' side='right'caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Harrison, J A]]		[[Category: Harrison, J A]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 06:48, 3 January 2018

OCA — Wed, 03 Jan 2018 06:48:27 GMT

←Older revision		Revision as of 06:48, 3 January 2018
Line 1:		Line 1:
		+
	==MopII from Clostridium pasteurianum complexed with tungstate==		==MopII from Clostridium pasteurianum complexed with tungstate==
	<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://pdbe.org/1gug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://pdbe.org/1gug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gug ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/1gug_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/1gug_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:29, 8 February 2016

OCA — Mon, 08 Feb 2016 22:29:51 GMT

←Older revision		Revision as of 22:29, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gug ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:58, 10 September 2015

OCA — Thu, 10 Sep 2015 15:58:18 GMT

←Older revision		Revision as of 15:58, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_pasteurianum Clostridium pasteurianum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pasteurianus"_(winogradsky_1895)_lehmann_and_neumann_1907 "bacillus pasteurianus" (winogradsky 1895) lehmann and neumann 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://pdbe.org/1gug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1gug" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium pasteurianum]]
	[[Category: Harrison, J A]]		[[Category: Harrison, J A]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 02:33, 25 December 2014

OCA — Thu, 25 Dec 2014 02:33:49 GMT

←Older revision		Revision as of 02:33, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA]] Binds one mole of molybdenum per mole of protein and contains a pterin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 00:42, 23 December 2014

OCA — Tue, 23 Dec 2014 00:42:08 GMT

←Older revision		Revision as of 00:42, 23 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium pasteurianum]]		[[Category: Clostridium pasteurianum]]
-	[[Category: Harrison, J A.]]	+	[[Category: Harrison, J A]]
-	[[Category: Hunter, W N.]]	+	[[Category: Hunter, W N]]
-	[[Category: Schuettelkopf, A W.]]	+	[[Category: Schuettelkopf, A W]]
	[[Category: Molbindin]]		[[Category: Molbindin]]
	[[Category: Molybdate binding]]		[[Category: Molybdate binding]]
	[[Category: Molybdate binding protein]]		[[Category: Molybdate binding protein]]
	[[Category: Mop]]		[[Category: Mop]]

OCA at 11:51, 3 October 2014

OCA — Fri, 03 Oct 2014 11:51:24 GMT

←Older revision		Revision as of 11:51, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1gug\| PDB~~=1gug \| ~~SCENE~~= }}	+	==MopII from Clostridium pasteurianum complexed with tungstate==
-	===~~MopII~~ from Clostridium pasteurianum ~~complexed with tungstate~~===	+	<StructureSection load='1gug' size='340' side='right' caption='[[1gug]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~{{ABSTRACT_PUBMED_11836258}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1gug]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gun\|1gun]], [[1guo\|1guo]], [[1gus\|1gus]], [[1gut\|1gut]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gug OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1gug RCSB], [http://www.ebi.ac.uk/pdbsum/1gug PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gu/1gug_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MopII from Clostridium pasteurianum is a molbindin family member. These proteins may serve as intracellular storage facilities for molybdate, which they bind with high specificity. High resolution structures of MopII in a number of states, including the first structure of an apo-molbindin, together with calorimetric data, allow us to describe ligand binding and provide support for the proposed storage function of the protein. MopII assembles as a trimer of dimers and binds eight oxyanions at two types of binding sites located at intersubunit interfaces. Two type 1 sites are on the molecular 3-fold axis and three pairs of type 2 sites occur on the molecular 2-fold axes. The hexamer is largely unaffected by the binding of ligand. Molybdate is admitted to the otherwise inaccessible type 2 binding sites by the movement of the N-terminal residues of each protein chain. This contrasts with the structurally related molybdate-dependent transcriptional regulator ModE, which undergoes extensive conformational rearrangements on ligand binding. Despite similarities between the binding sites of ModE and the type 2 sites of MopII the molbindin has a significantly reduced ligand affinity, due, in part, to the high density of negative charges at the center of the hexamer. In the absence of ligand this effects the movement of an important lysine side chain, thereby partially inactivating the binding sites. The differences are consistent with a biological role in molybdate storage/buffering.

-	~~==Function==~~	+	Passive acquisition of ligand by the MopII molbindin from Clostridium pasteurianum: structures of apo and oxyanion-bound forms.,Schuttelkopf AW, Harrison JA, Boxer DH, Hunter WN J Biol Chem. 2002 Apr 26;277(17):15013-20. Epub 2002 Feb 8. PMID:11836258<ref>PMID:11836258</ref>
-	~~[[http~~:~~//www.uniprot.org/uniprot/MOP2_CLOPA MOP2_CLOPA]] Binds one mole~~ of ~~molybdenum per mole of protein~~ and ~~contains a pterin~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1gug]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1GUG OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:011836258</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Clostridium pasteurianum]]		[[Category: Clostridium pasteurianum]]
	[[Category: Harrison, J A.]]		[[Category: Harrison, J A.]]