1hh1 - Revision history

OCA at 08:56, 9 May 2024

OCA — Thu, 09 May 2024 08:56:09 GMT

←Older revision		Revision as of 08:56, 9 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 2.15-A structure of Hjc, a Holliday junction-resolving enzyme from the archaeon Sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type II restriction enzymes. Hjc is a dimer with a large DNA-binding surface consisting of numerous basic residues surrounding the metal-binding residues of the active sites. Residues critical for catalysis, identified on the basis of sequence comparisons and site-directed mutagenesis studies, are clustered to produce two active sites in the dimer, about 29 A apart, consistent with the requirement for the introduction of paired nicks in opposing strands of the four-way DNA junction substrate. Hjc displays similarity to the restriction endonucleases in the way its specific DNA-cutting pattern is determined but uses a different arrangement of nuclease subunits. Further structural similarity to a broad group of metal/phosphate-binding proteins, including conservation of active-site location, is observed. A high degree of conservation of surface electrostatic character is observed between Hjc and T4-phage endonuclease VII despite a complete lack of structural homology. A model of the Hjc-Holliday junction complex is proposed, based on the available functional and structural data.
		+
		+	Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus.,Bond CS, Kvaratskhelia M, Richard D, White MF, Hunter WN Proc Natl Acad Sci U S A. 2001 May 8;98(10):5509-14. Epub 2001 May 1. PMID:11331763<ref>PMID:11331763</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 11:31, 27 March 2024

OCA — Wed, 27 Mar 2024 11:31:46 GMT

←Older revision		Revision as of 11:31, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35091 Atcc 35091~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [https://pdbe.org/1hh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [https://pdbe.org/1hh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~HJC_SULSO HJC_SULSO]~~] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>	+	[https://www.uniprot.org/uniprot/HJC_SACS2 HJC_SACS2] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The 2.15-A structure of Hjc, a Holliday junction-resolving enzyme from the archaeon Sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type II restriction enzymes. Hjc is a dimer with a large DNA-binding surface consisting of numerous basic residues surrounding the metal-binding residues of the active sites. Residues critical for catalysis, identified on the basis of sequence comparisons and site-directed mutagenesis studies, are clustered to produce two active sites in the dimer, about 29 A apart, consistent with the requirement for the introduction of paired nicks in opposing strands of the four-way DNA junction substrate. Hjc displays similarity to the restriction endonucleases in the way its specific DNA-cutting pattern is determined but uses a different arrangement of nuclease subunits. Further structural similarity to a broad group of metal/phosphate-binding proteins, including conservation of active-site location, is observed. A high degree of conservation of surface electrostatic character is observed between Hjc and T4-phage endonuclease VII despite a complete lack of structural homology. A model of the Hjc-Holliday junction complex is proposed, based on the available functional and structural data.
-
-	Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus.,Bond CS, Kvaratskhelia M, Richard D, White MF, Hunter WN Proc Natl Acad Sci U S A. 2001 May 8;98(10):5509-14. Epub 2001 May 1. PMID:11331763<ref>PMID:11331763</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35091]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bond, C S~~]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: ~~Hunter, W N~~]]	+	[[Category: Bond CS]]
-	[[Category: ~~Kvaratskhelia, M~~]]	+	[[Category: Hunter WN]]
-	[[Category: ~~Richard, D]]~~	+	[[Category: Kvaratskhelia M]]
-	~~[[Category: White,~~ M F]]	+	[[Category: Richard D]]
-	[[Category: ~~Archaea~~]]	+	[[Category: White MF]]
-	[[Category: ~~Holliday junction resolvase]]~~	+
-	~~[[Category: Homologous recombination]]~~	+
-	~~[[Category: Nuclease domain~~]]	+

OCA at 10:49, 4 August 2021

OCA — Wed, 04 Aug 2021 10:49:27 GMT

←Older revision		Revision as of 10:49, 4 August 2021
Line 3:		Line 3:
	<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [~~http~~://pdbe.org/1hh1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [https://pdbe.org/1hh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HJC_SULSO HJC_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>	+	[[https://www.uniprot.org/uniprot/HJC_SULSO HJC_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Resolvase\|Resolvase]]	+	*[[Resolvase 3D structures\|Resolvase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:52, 30 October 2019

OCA — Wed, 30 Oct 2019 09:52:07 GMT

←Older revision		Revision as of 09:52, 30 October 2019
Line 1:		Line 1:

	==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==		==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==
-	<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Resolvase\|Resolvase]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 35091]]		[[Category: Atcc 35091]]
		+	[[Category: Large Structures]]
	[[Category: Bond, C S]]		[[Category: Bond, C S]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 08:51, 10 January 2018

OCA — Wed, 10 Jan 2018 08:51:32 GMT

←Older revision		Revision as of 08:51, 10 January 2018
Line 1:		Line 1:
		+
	==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==		==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==
	<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://pdbe.org/1hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://pdbe.org/1hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hh1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hh1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Resolvase\|Resolvase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:09, 9 February 2016

OCA — Tue, 09 Feb 2016 16:09:57 GMT

←Older revision		Revision as of 16:09, 9 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:53, 10 September 2015

OCA — Thu, 10 Sep 2015 23:53:16 GMT

←Older revision		Revision as of 23:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_solfataricus Sulfolobus solfataricus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://pdbe.org/1hh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HJC_SULSO HJC_SULSO]] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 30:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sulfolobus solfataricus~~]]	+	[[Category: Atcc 35091]]
	[[Category: Bond, C S]]		[[Category: Bond, C S]]
	[[Category: Hunter, W N]]		[[Category: Hunter, W N]]

OCA at 20:03, 22 December 2014

OCA — Mon, 22 Dec 2014 20:03:01 GMT

←Older revision		Revision as of 20:03, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
-	[[Category: Bond, C S.]]	+	[[Category: Bond, C S]]
-	[[Category: Hunter, W N.]]	+	[[Category: Hunter, W N]]
-	[[Category: Kvaratskhelia, M.]]	+	[[Category: Kvaratskhelia, M]]
-	[[Category: Richard, D.]]	+	[[Category: Richard, D]]
-	[[Category: White, M F.]]	+	[[Category: White, M F]]
	[[Category: Archaea]]		[[Category: Archaea]]
	[[Category: Holliday junction resolvase]]		[[Category: Holliday junction resolvase]]
	[[Category: Homologous recombination]]		[[Category: Homologous recombination]]
	[[Category: Nuclease domain]]		[[Category: Nuclease domain]]

OCA at 09:53, 28 September 2014

OCA — Sun, 28 Sep 2014 09:53:41 GMT

←Older revision		Revision as of 09:53, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1hh1.~~png\|left~~\|200px]]	+	==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==
		+	<StructureSection load='1hh1' size='340' side='right' caption='[[1hh1]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1hh1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hh1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 2.15-A structure of Hjc, a Holliday junction-resolving enzyme from the archaeon Sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type II restriction enzymes. Hjc is a dimer with a large DNA-binding surface consisting of numerous basic residues surrounding the metal-binding residues of the active sites. Residues critical for catalysis, identified on the basis of sequence comparisons and site-directed mutagenesis studies, are clustered to produce two active sites in the dimer, about 29 A apart, consistent with the requirement for the introduction of paired nicks in opposing strands of the four-way DNA junction substrate. Hjc displays similarity to the restriction endonucleases in the way its specific DNA-cutting pattern is determined but uses a different arrangement of nuclease subunits. Further structural similarity to a broad group of metal/phosphate-binding proteins, including conservation of active-site location, is observed. A high degree of conservation of surface electrostatic character is observed between Hjc and T4-phage endonuclease VII despite a complete lack of structural homology. A model of the Hjc-Holliday junction complex is proposed, based on the available functional and structural data.

-	~~{{STRUCTURE_1hh1\| PDB=1hh1 \| SCENE= }}~~	+	Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus.,Bond CS, Kvaratskhelia M, Richard D, White MF, Hunter WN Proc Natl Acad Sci U S A. 2001 May 8;98(10):5509-14. Epub 2001 May 1. PMID:11331763<ref>PMID:11331763</ref>

-	~~===THE STRUCTURE OF HJC~~, ~~A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11331763}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1hh1]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA].~~	+

	==See Also==		==See Also==
	*[[Resolvase\|Resolvase]]		*[[Resolvase\|Resolvase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011331763<~~/~~ref~~><~~ref group="xtra">PMID:012499561</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Sulfolobus solfataricus]]		[[Category: Sulfolobus solfataricus]]
	[[Category: Bond, C S.]]		[[Category: Bond, C S.]]

OCA at 14:24, 20 October 2012

OCA — Sat, 20 Oct 2012 14:24:16 GMT

←Older revision		Revision as of 14:24, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1hh1]] is a 1 chain structure ~~of [[Resolvase]]~~ with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA].	+	[[1hh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA].

	==See Also==		==See Also==