1hkt - Revision history

OCA at 08:32, 22 May 2024

OCA — Wed, 22 May 2024 08:32:01 GMT

←Older revision		Revision as of 08:32, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of the DNA-binding domain of the Drosophila heat shock transcription factor, as determined by multidimensional multinuclear NMR, resembles that of the helix-turn-helix class of DNA-binding proteins. The domain comprises a four-stranded antiparallel beta-sheet, packed against a three-helix bundle. The second helix is significantly distorted and is separated from the third helix by an extended turn which is subject to conformational averaging on an intermediate time scale. Helix 3 forms a classical amphipathic helix with polar and charged residues exposed to the solvent. Upon titration with DNA, resonance shifts in the backbone and Asn and Gln side-chain amides indicate that helix 3 acts as the recognition helix of the heat shock transcription factor.
		+
		+	Solution structure of the DNA-binding domain of Drosophila heat shock transcription factor.,Vuister GW, Kim SJ, Orosz A, Marquardt J, Wu C, Bax A Nat Struct Biol. 1994 Sep;1(9):605-14. PMID:7634100<ref>PMID:7634100</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Heat shock factor\|Heat shock factor]]		*[[Heat shock factor\|Heat shock factor]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:32, 27 March 2024

OCA — Wed, 27 Mar 2024 11:32:48 GMT

←Older revision		Revision as of 11:32, 27 March 2024
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==		==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==
-	<StructureSection load='1hkt' size='340' side='right'caption='[[1hkt~~]], [[NMR_Ensembles_of_Models \| 28 NMR models~~]]' scene=''>	+	<StructureSection load='1hkt' size='340' side='right'caption='[[1hkt]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1hks\|1hks]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [https://pdbe.org/1hkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [https://www.ebi.ac.uk/pdbsum/1hkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [https://pdbe.org/1hkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [https://www.ebi.ac.uk/pdbsum/1hkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HSF_DROME HSF_DROME]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.	+	[https://www.uniprot.org/uniprot/HSF_DROME HSF_DROME] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The solution structure of the DNA-binding domain of the Drosophila heat shock transcription factor, as determined by multidimensional multinuclear NMR, resembles that of the helix-turn-helix class of DNA-binding proteins. The domain comprises a four-stranded antiparallel beta-sheet, packed against a three-helix bundle. The second helix is significantly distorted and is separated from the third helix by an extended turn which is subject to conformational averaging on an intermediate time scale. Helix 3 forms a classical amphipathic helix with polar and charged residues exposed to the solvent. Upon titration with DNA, resonance shifts in the backbone and Asn and Gln side-chain amides indicate that helix 3 acts as the recognition helix of the heat shock transcription factor.
-
-	Solution structure of the DNA-binding domain of Drosophila heat shock transcription factor.,Vuister GW, Kim SJ, Orosz A, Marquardt J, Wu C, Bax A Nat Struct Biol. 1994 Sep;1(9):605-14. PMID:7634100<ref>PMID:7634100</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Heat shock factor\|Heat shock factor]]		*[[Heat shock factor\|Heat shock factor]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bax, A]]	+	[[Category: Bax A]]
-	[[Category: Kim, S J]]	+	[[Category: Kim S-J]]
-	[[Category: Marquardt~~, J L~~]]	+	[[Category: Marquardt JL]]
-	[[Category: Orosz, A]]	+	[[Category: Orosz A]]
-	[[Category: Vuister~~, G W~~]]	+	[[Category: Vuister GW]]
-	[[Category: Wu, C]]	+	[[Category: Wu C]]
-	~~[[Category: Transcription regulation~~]]	+

OCA at 10:50, 4 August 2021

OCA — Wed, 04 Aug 2021 10:50:21 GMT

←Older revision		Revision as of 10:50, 4 August 2021
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	<StructureSection load='1hkt' size='340' side='right'caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>		<StructureSection load='1hkt' size='340' side='right'caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hks\|1hks]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [~~http~~://pdbe.org/1hkt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1hkt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [https://pdbe.org/1hkt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [https://www.ebi.ac.uk/pdbsum/1hkt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HSF_DROME HSF_DROME]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.	+	[[https://www.uniprot.org/uniprot/HSF_DROME HSF_DROME]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 09:59, 30 October 2019

OCA — Wed, 30 Oct 2019 09:59:25 GMT

←Older revision		Revision as of 09:59, 30 October 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==		==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==
-	<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>	+	<StructureSection load='1hkt' size='340' side='right'caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat shock factor\|Heat shock factor]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Bax, A]]		[[Category: Bax, A]]
	[[Category: Kim, S J]]		[[Category: Kim, S J]]

OCA at 08:59, 10 January 2018

OCA — Wed, 10 Jan 2018 08:59:35 GMT

←Older revision		Revision as of 08:59, 10 January 2018
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==		==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==
	<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>		<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://pdbe.org/1hkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://pdbe.org/1hkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Heat shock factor\|Heat shock factor]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:53, 8 February 2016

OCA — Mon, 08 Feb 2016 10:53:23 GMT

←Older revision		Revision as of 10:53, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:50, 10 September 2015

OCA — Thu, 10 Sep 2015 10:50:40 GMT

←Older revision		Revision as of 10:50, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>		<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://pdbe.org/1hkt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1hkt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Bax, A]]		[[Category: Bax, A]]
	[[Category: Kim, S J]]		[[Category: Kim, S J]]

OCA at 22:49, 24 December 2014

OCA — Wed, 24 Dec 2014 22:49:40 GMT

←Older revision		Revision as of 22:49, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HSF_DROME HSF_DROME]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:19, 22 December 2014

OCA — Mon, 22 Dec 2014 19:19:10 GMT

←Older revision		Revision as of 19:19, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Bax, A.]]	+	[[Category: Bax, A]]
-	[[Category: Kim, S J.]]	+	[[Category: Kim, S J]]
-	[[Category: Marquardt, J L.]]	+	[[Category: Marquardt, J L]]
-	[[Category: Orosz, A.]]	+	[[Category: Orosz, A]]
-	[[Category: Vuister, G W.]]	+	[[Category: Vuister, G W]]
-	[[Category: Wu, C.]]	+	[[Category: Wu, C]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]

OCA at 09:15, 28 September 2014

OCA — Sun, 28 Sep 2014 09:15:29 GMT

←Older revision		Revision as of 09:15, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1hkt.~~png~~\|~~left~~\|200px]]	+	==SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR==
		+	<StructureSection load='1hkt' size='340' side='right' caption='[[1hkt]], [[NMR_Ensembles_of_Models \| 28 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1hkt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HKT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hks\|1hks]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hkt RCSB], [http://www.ebi.ac.uk/pdbsum/1hkt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The solution structure of the DNA-binding domain of the Drosophila heat shock transcription factor, as determined by multidimensional multinuclear NMR, resembles that of the helix-turn-helix class of DNA-binding proteins. The domain comprises a four-stranded antiparallel beta-sheet, packed against a three-helix bundle. The second helix is significantly distorted and is separated from the third helix by an extended turn which is subject to conformational averaging on an intermediate time scale. Helix 3 forms a classical amphipathic helix with polar and charged residues exposed to the solvent. Upon titration with DNA, resonance shifts in the backbone and Asn and Gln side-chain amides indicate that helix 3 acts as the recognition helix of the heat shock transcription factor.

-	~~{{STRUCTURE_1hkt\| PDB=1hkt \| SCENE= }}~~	+	Solution structure of the DNA-binding domain of Drosophila heat shock transcription factor.,Vuister GW, Kim SJ, Orosz A, Marquardt J, Wu C, Bax A Nat Struct Biol. 1994 Sep;1(9):605-14. PMID:7634100<ref>PMID:7634100</ref>

-	~~===SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN OF DROSOPHILA HEAT SHOCK TRANSCRIPTION FACTOR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7634100}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1hkt]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Drosophila_melanogaster Drosophila melanogaster]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HKT OCA].~~	+

	==See Also==		==See Also==
	*[[Heat shock factor\|Heat shock factor]]		*[[Heat shock factor\|Heat shock factor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:007634100<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Bax, A.]]		[[Category: Bax, A.]]