1htr - Revision history

OCA at 11:35, 27 March 2024

OCA — Wed, 27 Mar 2024 11:35:17 GMT

←Older revision		Revision as of 11:35, 27 March 2024
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	<StructureSection load='1htr' size='340' side='right'caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='1htr' size='340' side='right'caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Gastricsin Gastricsin~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.62Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [https://pdbe.org/1htr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [https://www.ebi.ac.uk/pdbsum/1htr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1htr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [https://pdbe.org/1htr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [https://www.ebi.ac.uk/pdbsum/1htr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1htr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PEPC_HUMAN PEPC_HUMAN]] Hydrolyzes a variety of proteins.	+	[https://www.uniprot.org/uniprot/PEPC_HUMAN PEPC_HUMAN] Hydrolyzes a variety of proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1htr ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1htr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal and molecular structures of human progastricsin (hPGC) have been determined using multiple isomorphous replacement methods and anomalous scattering in conjunction with a phased translation function. The structure has been refined to a conventional R-factor (= sigma parallel Fo magnitude of - magnitude of Fc parallel / sigma magnitude of Fo magnitude of) of 0.179 with data to 1.62 A resolution. The first 37 amino acid residues of the prosegment are similar in conformation to the equivalent residues of porcine pepsinogen (pPGN). As in pPGN, the N zeta atom of Lys37p sits between the active-site carboxylate groups of Asp32 and Asp217, thereby preventing catalysis. The side-chains of Tyr38p and Tyr9 sit in the S1' and S1 substrate-binding pockets of hPGC, respectively, in an analogous manner to what is observed in porcine pepsinogen. There are large conformational differences centered around the region containing residues Arg39p to Pro6, relative to the equivalent region in the structure of pPGN. Two surface loops in the vicinity of this segment are also displaced relative to those in pPGN and in mature aspartic proteinases (Phe71 to Thr81 (the "flap"), and Tyr125 to Thr131). In hPGC, Tyr75 O eta does not make its usual hydrogen bond to Trp39 N epsilon 1. Rather, the "flap" containing Tyr75 is excluded from the active site by the polypeptide segment Arg39p to Pro6. However, the conformation of the inhibitory segment, Lys37p to Tyr38p, is virtually identical with that observed in pPGN. Hence the structures of these two proteins indicate that aspartic proteinase zymogens keep themselves inactive at neutral pH by a very similar mechanism in human progastricsin and porcine pepsinogen. This similarity likely carries over to all members of both the pepsinogen A and C families of aspartic proteinase zymogens.
-
-	Crystal and molecular structures of human progastricsin at 1.62 A resolution.,Moore SA, Sielecki AR, Chernaia MM, Tarasova NI, James MN J Mol Biol. 1995 Mar 31;247(3):466-85. PMID:7714902<ref>PMID:7714902</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1htr" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gastricsin]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: James~~, M N.G~~]]	+	[[Category: James MNG]]
-	[[Category: Moore~~, S A~~]]	+	[[Category: Moore SA]]
-	[[Category: Sielecki~~, A R]]~~	+	[[Category: Sielecki AR]]
-	~~[[Category: Aspartyl protease~~]]	+

OCA at 10:53, 4 August 2021

OCA — Wed, 04 Aug 2021 10:53:29 GMT

←Older revision		Revision as of 10:53, 4 August 2021
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	<StructureSection load='1htr' size='340' side='right'caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='1htr' size='340' side='right'caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [~~http~~://pdbe.org/1htr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1htr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1htr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [https://pdbe.org/1htr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [https://www.ebi.ac.uk/pdbsum/1htr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1htr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PEPC_HUMAN PEPC_HUMAN]] Hydrolyzes a variety of proteins.	+	[[https://www.uniprot.org/uniprot/PEPC_HUMAN PEPC_HUMAN]] Hydrolyzes a variety of proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:13, 30 October 2019

OCA — Wed, 30 Oct 2019 10:13:53 GMT

←Older revision		Revision as of 10:13, 30 October 2019
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	==CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION==		==CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION==
-	<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>	+	<StructureSection load='1htr' size='340' side='right'caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
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	[[Category: Gastricsin]]		[[Category: Gastricsin]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: James, M N.G]]		[[Category: James, M N.G]]
	[[Category: Moore, S A]]		[[Category: Moore, S A]]
	[[Category: Sielecki, A R]]		[[Category: Sielecki, A R]]
	[[Category: Aspartyl protease]]		[[Category: Aspartyl protease]]

OCA at 09:14, 10 January 2018

OCA — Wed, 10 Jan 2018 09:14:53 GMT

←Older revision		Revision as of 09:14, 10 January 2018
Line 1:		Line 1:
		+
	==CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION==		==CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION==
	<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://pdbe.org/1htr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://pdbe.org/1htr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1htr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1htr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1htr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:13, 9 February 2016

OCA — Tue, 09 Feb 2016 01:13:32 GMT

←Older revision		Revision as of 01:13, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1htr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:10, 10 September 2015

OCA — Thu, 10 Sep 2015 17:10:32 GMT

←Older revision		Revision as of 17:10, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://pdbe.org/1htr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1htr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Gastricsin]]		[[Category: Gastricsin]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: James, M N.G]]		[[Category: James, M N.G]]
	[[Category: Moore, S A]]		[[Category: Moore, S A]]
	[[Category: Sielecki, A R]]		[[Category: Sielecki, A R]]
	[[Category: Aspartyl protease]]		[[Category: Aspartyl protease]]

OCA at 03:25, 25 December 2014

OCA — Thu, 25 Dec 2014 03:25:32 GMT

←Older revision		Revision as of 03:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PEPC_HUMAN PEPC_HUMAN]] Hydrolyzes a variety of proteins.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	[[Category: Gastricsin]]		[[Category: Gastricsin]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: James, M N.G.]]	+	[[Category: James, M N.G]]
-	[[Category: Moore, S A.]]	+	[[Category: Moore, S A]]
-	[[Category: Sielecki, A R.]]	+	[[Category: Sielecki, A R]]
	[[Category: Aspartyl protease]]		[[Category: Aspartyl protease]]

OCA at 09:27, 28 September 2014

OCA — Sun, 28 Sep 2014 09:27:17 GMT

←Older revision		Revision as of 09:27, 28 September 2014
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-	[[~~Image~~:1htr.~~png\|left~~\|200px]]	+	==CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION==
		+	<StructureSection load='1htr' size='340' side='right' caption='[[1htr]], [[Resolution\|resolution]] 1.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HTR FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Gastricsin Gastricsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.3 3.4.23.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1htr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1htr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1htr RCSB], [http://www.ebi.ac.uk/pdbsum/1htr PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1htr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal and molecular structures of human progastricsin (hPGC) have been determined using multiple isomorphous replacement methods and anomalous scattering in conjunction with a phased translation function. The structure has been refined to a conventional R-factor (= sigma parallel Fo magnitude of - magnitude of Fc parallel / sigma magnitude of Fo magnitude of) of 0.179 with data to 1.62 A resolution. The first 37 amino acid residues of the prosegment are similar in conformation to the equivalent residues of porcine pepsinogen (pPGN). As in pPGN, the N zeta atom of Lys37p sits between the active-site carboxylate groups of Asp32 and Asp217, thereby preventing catalysis. The side-chains of Tyr38p and Tyr9 sit in the S1' and S1 substrate-binding pockets of hPGC, respectively, in an analogous manner to what is observed in porcine pepsinogen. There are large conformational differences centered around the region containing residues Arg39p to Pro6, relative to the equivalent region in the structure of pPGN. Two surface loops in the vicinity of this segment are also displaced relative to those in pPGN and in mature aspartic proteinases (Phe71 to Thr81 (the "flap"), and Tyr125 to Thr131). In hPGC, Tyr75 O eta does not make its usual hydrogen bond to Trp39 N epsilon 1. Rather, the "flap" containing Tyr75 is excluded from the active site by the polypeptide segment Arg39p to Pro6. However, the conformation of the inhibitory segment, Lys37p to Tyr38p, is virtually identical with that observed in pPGN. Hence the structures of these two proteins indicate that aspartic proteinase zymogens keep themselves inactive at neutral pH by a very similar mechanism in human progastricsin and porcine pepsinogen. This similarity likely carries over to all members of both the pepsinogen A and C families of aspartic proteinase zymogens.

-	~~{{STRUCTURE_1htr\| PDB=1htr \| SCENE= }}~~	+	Crystal and molecular structures of human progastricsin at 1.62 A resolution.,Moore SA, Sielecki AR, Chernaia MM, Tarasova NI, James MN J Mol Biol. 1995 Mar 31;247(3):466-85. PMID:7714902<ref>PMID:7714902</ref>

-	~~===CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7714902}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1htr]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007714902<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Gastricsin]]		[[Category: Gastricsin]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]

OCA: Protected "1htr" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:57:22 GMT

Protected "1htr" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:57, 21 November 2012

OCA at 15:57, 21 November 2012

OCA — Wed, 21 Nov 2012 15:57:18 GMT

←Older revision		Revision as of 15:57, 21 November 2012
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-	{{Seed}}
	[[Image:1htr.png\|left\|200px]]		[[Image:1htr.png\|left\|200px]]

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	{{STRUCTURE_1htr\| PDB=1htr \| SCENE= }}		{{STRUCTURE_1htr\| PDB=1htr \| SCENE= }}

	===CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION===		===CRYSTAL AND MOLECULAR STRUCTURES OF HUMAN PROGASTRICSIN AT 1.62 ANGSTROMS RESOLUTION===

-
-	<!--
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	{{ABSTRACT_PUBMED_7714902}}		{{ABSTRACT_PUBMED_7714902}}

	==About this Structure==		==About this Structure==
-	~~1HTR~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA].	+	[[1htr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HTR OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~7714902~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007714902</ref><references group="xtra"/>
	[[Category: Gastricsin]]		[[Category: Gastricsin]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
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	[[Category: Sielecki, A R.]]		[[Category: Sielecki, A R.]]
	[[Category: Aspartyl protease]]		[[Category: Aspartyl protease]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:16:04 2009''