1hzk - Revision history

OCA at 07:43, 3 April 2024

OCA — Wed, 03 Apr 2024 07:43:20 GMT

←Older revision		Revision as of 07:43, 3 April 2024
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	==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==		==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==
-	<StructureSection load='1hzk' size='340' side='right'caption='[[1hzk~~]], [[NMR_Ensembles_of_Models \| 30 NMR models~~]]' scene=''>	+	<StructureSection load='1hzk' size='340' side='right'caption='[[1hzk]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1hzl\|1hzl]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [https://pdbe.org/1hzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [https://www.ebi.ac.uk/pdbsum/1hzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hzk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [https://pdbe.org/1hzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [https://www.ebi.ac.uk/pdbsum/1hzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CAGA_STRGL CAGA_STRGL]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.	+	[https://www.uniprot.org/uniprot/CAGA_STRGL CAGA_STRGL] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hzk ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hzk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	C-1027 is one of the most potent antitumor antibiotic chromoproteins, and is a 1:1 complex of an enediyne chromophore having DNA-cleaving ability and a carrier apoprotein. The three-dimensional solution structures of the 110 residue (10.5 kDa) C-1027 apoprotein and its complex with the aromatized chromophore have been determined separately by homonuclear two-dimensional nuclear magnetic resonance methods. The apoprotein is mainly composed of three antiparallel beta-sheets: four-stranded beta-sheet (43-45, 52-54; 30-38; 92-94; 104-106), three-stranded beta-sheet (4-6; 17-22; 61-66), and two-stranded beta-sheet (70-72; 83-85). The overall structure of the apoprotein is very similar to those of other chromoprotein apoproteins, such as neocarzinostatin and kedarcidin. A hydrophobic pocket with approximate dimensions of 14 A x 12 A x 8 A is formed by the four-stranded beta-sheet and the three loops (39-42; 75-79; 97-100). The holoprotein (complex form with the aromatized chromophore) structure reveals that the aromatized chromophore is bound to the hydrophobic pocket found in the apoprotein. The benzodihydropentalene core of the chromophore is located in the center of the pocket and other substituents (beta-tyrosine, benzoxazine, and aminosugar moieties) are arranged around the core. Major binding interactions between the apoprotein and the chromophore are likely the hydrophobic contacts between the core of the chromophore and the hydrophobic side-chains of the pocket-forming residues, which is supplemented by salt bridges and/or hydrogen bonds. Based on the holoprotein structure, we propose possible mechanisms for the stabilization and the release of chromophore by the apoprotein.
-
-	Solution structures of C-1027 apoprotein and its complex with the aromatized chromophore.,Tanaka T, Fukuda-Ishisaka S, Hirama M, Otani T J Mol Biol. 2001 May 25;309(1):267-83. PMID:11491295<ref>PMID:11491295</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1hzk" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
-	[[Category: Fukuda-Ishisaka, S]]	+	[[Category: Fukuda-Ishisaka S]]
-	[[Category: Hirama, M]]	+	[[Category: Hirama M]]
-	[[Category: Otani, T]]	+	[[Category: Otani T]]
-	[[Category: Tanaka, T]]	+	[[Category: Tanaka T]]
-	~~[[Category: Antibiotic]]~~	+
-	~~[[Category: Apoprotein]]~~	+
-	~~[[Category: C-1027]]~~	+
-	~~[[Category: Chromoprotein~~]]	+

OCA at 07:31, 7 April 2021

OCA — Wed, 07 Apr 2021 07:31:42 GMT

←Older revision		Revision as of 07:31, 7 April 2021
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	<StructureSection load='1hzk' size='340' side='right'caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='1hzk' size='340' side='right'caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1HZK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hzl\|1hzl]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [~~http~~://pdbe.org/1hzk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1hzk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1hzk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [https://pdbe.org/1hzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [https://www.ebi.ac.uk/pdbsum/1hzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CAGA_STRGL CAGA_STRGL]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.	+	[[https://www.uniprot.org/uniprot/CAGA_STRGL CAGA_STRGL]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:54, 3 July 2019

OCA — Wed, 03 Jul 2019 07:54:06 GMT

←Older revision		Revision as of 07:54, 3 July 2019
Line 1:		Line 1:

	==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==		==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==
-	<StructureSection load='1hzk' size='340' side='right' caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>	+	<StructureSection load='1hzk' size='340' side='right'caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/1hzk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/1hzk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
	[[Category: Fukuda-Ishisaka, S]]		[[Category: Fukuda-Ishisaka, S]]

OCA at 10:52, 27 September 2017

OCA — Wed, 27 Sep 2017 10:52:55 GMT

←Older revision		Revision as of 10:52, 27 September 2017
Line 1:		Line 1:
		+
	==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==		==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==
	<StructureSection load='1hzk' size='340' side='right' caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>		<StructureSection load='1hzk' size='340' side='right' caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://pdbe.org/1hzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://pdbe.org/1hzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hzk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hzk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 08:52, 11 September 2015

OCA — Fri, 11 Sep 2015 08:52:05 GMT

←Older revision		Revision as of 08:52, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://pdbe.org/1hzk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1hzk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:06, 25 December 2014

OCA — Thu, 25 Dec 2014 14:06:38 GMT

←Older revision		Revision as of 14:06, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>		<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAGA_STRGL CAGA_STRGL]] Binds non-covalently to a chromophore which is the cytotoxic and mutagenic component of the antibiotic. The chromophore binds to DNA as a weak intercalator and causes single- and double-strand breaks.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
-	[[Category: Fukuda-Ishisaka, S.]]	+	[[Category: Fukuda-Ishisaka, S]]
-	[[Category: Hirama, M.]]	+	[[Category: Hirama, M]]
-	[[Category: Otani, T.]]	+	[[Category: Otani, T]]
-	[[Category: Tanaka, T.]]	+	[[Category: Tanaka, T]]
	[[Category: Antibiotic]]		[[Category: Antibiotic]]
	[[Category: Apoprotein]]		[[Category: Apoprotein]]
	[[Category: C-1027]]		[[Category: C-1027]]
	[[Category: Chromoprotein]]		[[Category: Chromoprotein]]

OCA at 12:59, 28 September 2014

OCA — Sun, 28 Sep 2014 12:59:07 GMT

←Older revision		Revision as of 12:59, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1hzk.~~png~~\|~~left~~\|200px]]	+	==SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE==
		+	<StructureSection load='1hzk' size='340' side='right' caption='[[1hzk]], [[NMR_Ensembles_of_Models \| 30 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HZK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hzl\|1hzl]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hzk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1hzk RCSB], [http://www.ebi.ac.uk/pdbsum/1hzk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/1hzk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	C-1027 is one of the most potent antitumor antibiotic chromoproteins, and is a 1:1 complex of an enediyne chromophore having DNA-cleaving ability and a carrier apoprotein. The three-dimensional solution structures of the 110 residue (10.5 kDa) C-1027 apoprotein and its complex with the aromatized chromophore have been determined separately by homonuclear two-dimensional nuclear magnetic resonance methods. The apoprotein is mainly composed of three antiparallel beta-sheets: four-stranded beta-sheet (43-45, 52-54; 30-38; 92-94; 104-106), three-stranded beta-sheet (4-6; 17-22; 61-66), and two-stranded beta-sheet (70-72; 83-85). The overall structure of the apoprotein is very similar to those of other chromoprotein apoproteins, such as neocarzinostatin and kedarcidin. A hydrophobic pocket with approximate dimensions of 14 A x 12 A x 8 A is formed by the four-stranded beta-sheet and the three loops (39-42; 75-79; 97-100). The holoprotein (complex form with the aromatized chromophore) structure reveals that the aromatized chromophore is bound to the hydrophobic pocket found in the apoprotein. The benzodihydropentalene core of the chromophore is located in the center of the pocket and other substituents (beta-tyrosine, benzoxazine, and aminosugar moieties) are arranged around the core. Major binding interactions between the apoprotein and the chromophore are likely the hydrophobic contacts between the core of the chromophore and the hydrophobic side-chains of the pocket-forming residues, which is supplemented by salt bridges and/or hydrogen bonds. Based on the holoprotein structure, we propose possible mechanisms for the stabilization and the release of chromophore by the apoprotein.

-	~~{{STRUCTURE_1hzk\| PDB=1hzk \| SCENE= }}~~	+	Solution structures of C-1027 apoprotein and its complex with the aromatized chromophore.,Tanaka T, Fukuda-Ishisaka S, Hirama M, Otani T J Mol Biol. 2001 May 25;309(1):267-83. PMID:11491295<ref>PMID:11491295</ref>

-	~~===SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11491295}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1hzk]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_globisporus Streptomyces globisporus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011491295<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
	[[Category: Fukuda-Ishisaka, S.]]		[[Category: Fukuda-Ishisaka, S.]]

OCA: Protected "1hzk" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 16:22:13 GMT

Protected "1hzk" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:22, 21 November 2012

OCA at 16:22, 21 November 2012

OCA — Wed, 21 Nov 2012 16:22:10 GMT

←Older revision		Revision as of 16:22, 21 November 2012
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-	{{Seed}}
	[[Image:1hzk.png\|left\|200px]]		[[Image:1hzk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1hzk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1hzk\| PDB=1hzk \| SCENE= }}		{{STRUCTURE_1hzk\| PDB=1hzk \| SCENE= }}

	===SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE===		===SOLUTION STRUCTURES OF C-1027 APOPROTEIN AND ITS COMPLEX WITH THE AROMATIZED CHROMOPHORE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11491295}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11491295 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11491295}}		{{ABSTRACT_PUBMED_11491295}}

	==About this Structure==		==About this Structure==
-	~~1HZK~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA].	+	[[1hzk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11491295~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011491295</ref><references group="xtra"/>
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
	[[Category: Fukuda-Ishisaka, S.]]		[[Category: Fukuda-Ishisaka, S.]]
Line 29:		Line 17:
	[[Category: Otani, T.]]		[[Category: Otani, T.]]
	[[Category: Tanaka, T.]]		[[Category: Tanaka, T.]]
		+	[[Category: Antibiotic]]
	[[Category: Apoprotein]]		[[Category: Apoprotein]]
	[[Category: C-1027]]		[[Category: C-1027]]
	[[Category: Chromoprotein]]		[[Category: Chromoprotein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:14:48 2009''

OCA at 16:14, 17 February 2009

OCA — Tue, 17 Feb 2009 16:14:50 GMT

←Older revision		Revision as of 16:14, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1HZK is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA].	+	1HZK is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_globisporus Streptomyces globisporus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HZK OCA].

	==Reference==		==Reference==
-	~~Solution structures of C-1027 apoprotein and its complex with the aromatized chromophore., Tanaka T, Fukuda-Ishisaka S, Hirama M, Otani T, J Mol Biol. 2001 May 25;309(1):267-83.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11491295 11491295]~~	+	<ref group="xtra">PMID:11491295</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Streptomyces globisporus]]		[[Category: Streptomyces globisporus]]
	[[Category: Fukuda-Ishisaka, S.]]		[[Category: Fukuda-Ishisaka, S.]]
Line 34:		Line 33:
	[[Category: Chromoprotein]]		[[Category: Chromoprotein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 1 10~~:11:~~00 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:14:48 2009''