1i5p - Revision history

OCA at 07:44, 3 April 2024

OCA — Wed, 03 Apr 2024 07:44:25 GMT

←Older revision		Revision as of 07:44, 3 April 2024
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	<StructureSection load='1i5p' size='340' side='right'caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1i5p' size='340' side='right'caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bactk Bactk~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1I5P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CRY2AA OPERON (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=29339 BACTK])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [~~http~~://pdbe.org/1i5p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1i5p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [https://pdbe.org/1i5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [https://www.ebi.ac.uk/pdbsum/1i5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CR2AA_BACTK CR2AA_BACTK]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae.	+	[https://www.uniprot.org/uniprot/CR2AA_BACTK CR2AA_BACTK] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i5p ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i5p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Genetically modified (GM) crops that express insecticidal protein toxins are an integral part of modern agriculture. Proteins produced by Bacillus thuringiensis (Bt) during sporulation mediate the pathogenicity of Bt toward a spectrum of insect larvae whose breadth depends upon the Bt strain. These transmembrane channel-forming toxins are stored in Bt as crystalline inclusions called Cry proteins. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. Though Bt toxins are promising as a crop protection alternative and are ecologically friendlier than synthetic organic pesticides, resistance to Bt toxins by insects is recognized as a potential limitation to their application. RESULTS: We have determined the 2.2 A crystal structure of the Cry2Aa protoxin by multiple isomorphous replacement. This is the first crystal structure of a Cry toxin specific to Diptera (mosquitoes and flies) and the first structure of a Cry toxin with high activity against larvae from two insect orders, Lepidoptera (moths and butterflies) and Diptera. Cry2Aa also provides the first structure of the proregion of a Cry toxin that is cleaved to generate the membrane-active toxin in the larval gut. CONCLUSIONS: The crystal structure of Cry2Aa reported here, together with chimeric-scanning and domain-swapping mutagenesis, defines the putative receptor binding epitope on the toxin and so may allow for alteration of specificity to combat resistance or to minimize collateral effects on nontarget species. The putative receptor binding epitope of Cry2Aa identified in this study differs from that inferred from previous structural studies of other Cry toxins.
-
-	Structure of Cry2Aa suggests an unexpected receptor binding epitope.,Morse RJ, Yamamoto T, Stroud RM Structure. 2001 May 9;9(5):409-17. PMID:11377201<ref>PMID:11377201</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1i5p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Delta-endotoxin\|Delta-endotoxin]]		*[[Delta-endotoxin\|Delta-endotoxin]]
	*[[Pesticidal crystal protein\|Pesticidal crystal protein]]		*[[Pesticidal crystal protein\|Pesticidal crystal protein]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bactk~~]]	+	[[Category: Bacillus thuringiensis serovar kurstaki]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Morse~~, R J~~]]	+	[[Category: Morse RJ]]
-	[[Category: Stroud~~, R M~~]]	+	[[Category: Stroud RM]]
-	[[Category: Yamamoto, T]]	+	[[Category: Yamamoto T]]
-	~~[[Category: Beta prism]]~~	+
-	~~[[Category: Helical bundle]]~~	+
-	~~[[Category: Jelly roll]]~~	+
-	~~[[Category: Lectin-like beta sandwich]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:36, 18 November 2020

OCA — Wed, 18 Nov 2020 10:36:27 GMT

←Older revision		Revision as of 10:36, 18 November 2020
Line 1:		Line 1:

	==INSECTICIDAL CRYSTAL PROTEIN CRY2AA==		==INSECTICIDAL CRYSTAL PROTEIN CRY2AA==
-	<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1i5p' size='340' side='right'caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactk Bactk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1I5P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactk Bactk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 BACTK])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 BACTK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://pdbe.org/1i5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://pdbe.org/1i5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1i5p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1i5p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Delta-endotoxin\|Delta-endotoxin]]
		+	*[[Pesticidal crystal protein\|Pesticidal crystal protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bactk]]		[[Category: Bactk]]
		+	[[Category: Large Structures]]
	[[Category: Morse, R J]]		[[Category: Morse, R J]]
	[[Category: Stroud, R M]]		[[Category: Stroud, R M]]

OCA at 09:33, 10 January 2018

OCA — Wed, 10 Jan 2018 09:33:41 GMT

←Older revision		Revision as of 09:33, 10 January 2018
Line 1:		Line 1:
		+
	==INSECTICIDAL CRYSTAL PROTEIN CRY2AA==		==INSECTICIDAL CRYSTAL PROTEIN CRY2AA==
	<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactk Bactk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactk Bactk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 BACTK])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 BACTK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://pdbe.org/1i5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://pdbe.org/1i5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i5p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i5p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:38, 7 February 2016

OCA — Sun, 07 Feb 2016 03:38:44 GMT

←Older revision		Revision as of 03:38, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i5p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:24, 9 September 2015

OCA — Wed, 09 Sep 2015 21:24:12 GMT

←Older revision		Revision as of 21:24, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactk Bactk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 ~~Bacillus thuringiensis serovar kurstaki~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 BACTK])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://pdbe.org/1i5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1i5p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus thuringiensis serovar kurstaki~~]]	+	[[Category: Bactk]]
	[[Category: Morse, R J]]		[[Category: Morse, R J]]
	[[Category: Stroud, R M]]		[[Category: Stroud, R M]]

OCA at 07:34, 24 December 2014

OCA — Wed, 24 Dec 2014 07:34:40 GMT

←Older revision		Revision as of 07:34, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 Bacillus thuringiensis serovar kurstaki])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 Bacillus thuringiensis serovar kurstaki])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CR2AA_BACTK CR2AA_BACTK]] Promotes colloidosmotic lysis by binding to the midgut epithelial cells of both dipteran (Aedes aegypti) and lepidopteran (Manduca sexta) larvae.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus thuringiensis serovar kurstaki]]		[[Category: Bacillus thuringiensis serovar kurstaki]]
-	[[Category: Morse, R J.]]	+	[[Category: Morse, R J]]
-	[[Category: Stroud, R M.]]	+	[[Category: Stroud, R M]]
-	[[Category: Yamamoto, T.]]	+	[[Category: Yamamoto, T]]
	[[Category: Beta prism]]		[[Category: Beta prism]]
	[[Category: Helical bundle]]		[[Category: Helical bundle]]

OCA at 09:03, 28 September 2014

OCA — Sun, 28 Sep 2014 09:03:13 GMT

←Older revision		Revision as of 09:03, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1i5p.~~png\|left~~\|200px]]	+	==INSECTICIDAL CRYSTAL PROTEIN CRY2AA==
		+	<StructureSection load='1i5p' size='340' side='right' caption='[[1i5p]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I5P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CRY2AA OPERON ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29339 Bacillus thuringiensis serovar kurstaki])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i5p RCSB], [http://www.ebi.ac.uk/pdbsum/1i5p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i5p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Genetically modified (GM) crops that express insecticidal protein toxins are an integral part of modern agriculture. Proteins produced by Bacillus thuringiensis (Bt) during sporulation mediate the pathogenicity of Bt toward a spectrum of insect larvae whose breadth depends upon the Bt strain. These transmembrane channel-forming toxins are stored in Bt as crystalline inclusions called Cry proteins. These proteins are the active agents used in the majority of biorational pesticides and insect-resistant transgenic crops. Though Bt toxins are promising as a crop protection alternative and are ecologically friendlier than synthetic organic pesticides, resistance to Bt toxins by insects is recognized as a potential limitation to their application. RESULTS: We have determined the 2.2 A crystal structure of the Cry2Aa protoxin by multiple isomorphous replacement. This is the first crystal structure of a Cry toxin specific to Diptera (mosquitoes and flies) and the first structure of a Cry toxin with high activity against larvae from two insect orders, Lepidoptera (moths and butterflies) and Diptera. Cry2Aa also provides the first structure of the proregion of a Cry toxin that is cleaved to generate the membrane-active toxin in the larval gut. CONCLUSIONS: The crystal structure of Cry2Aa reported here, together with chimeric-scanning and domain-swapping mutagenesis, defines the putative receptor binding epitope on the toxin and so may allow for alteration of specificity to combat resistance or to minimize collateral effects on nontarget species. The putative receptor binding epitope of Cry2Aa identified in this study differs from that inferred from previous structural studies of other Cry toxins.

-	~~{{STRUCTURE_1i5p\| PDB=1i5p \| SCENE= }}~~	+	Structure of Cry2Aa suggests an unexpected receptor binding epitope.,Morse RJ, Yamamoto T, Stroud RM Structure. 2001 May 9;9(5):409-17. PMID:11377201<ref>PMID:11377201</ref>

-	~~===INSECTICIDAL CRYSTAL PROTEIN CRY2AA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11377201}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1i5p]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011377201<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus thuringiensis serovar kurstaki]]		[[Category: Bacillus thuringiensis serovar kurstaki]]
	[[Category: Morse, R J.]]		[[Category: Morse, R J.]]

OCA: Protected "1i5p" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 16:21:30 GMT

Protected "1i5p" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:21, 21 November 2012

OCA at 16:21, 21 November 2012

OCA — Wed, 21 Nov 2012 16:21:28 GMT

←Older revision		Revision as of 16:21, 21 November 2012
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-	{{Seed}}
	[[Image:1i5p.png\|left\|200px]]		[[Image:1i5p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1i5p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1i5p\| PDB=1i5p \| SCENE= }}		{{STRUCTURE_1i5p\| PDB=1i5p \| SCENE= }}

	===INSECTICIDAL CRYSTAL PROTEIN CRY2AA===		===INSECTICIDAL CRYSTAL PROTEIN CRY2AA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11377201}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11377201 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11377201}}		{{ABSTRACT_PUBMED_11377201}}

	==About this Structure==		==About this Structure==
-	~~1I5P~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA].	+	[[1i5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11377201~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011377201</ref><references group="xtra"/>
	[[Category: Bacillus thuringiensis serovar kurstaki]]		[[Category: Bacillus thuringiensis serovar kurstaki]]
	[[Category: Morse, R J.]]		[[Category: Morse, R J.]]
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	[[Category: Jelly roll]]		[[Category: Jelly roll]]
	[[Category: Lectin-like beta sandwich]]		[[Category: Lectin-like beta sandwich]]
-		+	[[Category: Toxin]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 10:54:21 2009''~~	+

OCA at 08:54, 16 February 2009

OCA — Mon, 16 Feb 2009 08:54:24 GMT

←Older revision		Revision as of 08:54, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1I5P is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA].	+	1I5P is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis_serovar_kurstaki Bacillus thuringiensis serovar kurstaki]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5P OCA].

	==Reference==		==Reference==
-	~~Structure of Cry2Aa suggests an unexpected receptor binding epitope., Morse RJ, Yamamoto T, Stroud RM, Structure. 2001 May 9;9(5):409-17.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11377201 11377201]~~	+	<ref group="xtra">PMID:11377201</ref><references group="xtra"/>
	[[Category: Bacillus thuringiensis serovar kurstaki]]		[[Category: Bacillus thuringiensis serovar kurstaki]]
-	[[Category: Single protein]]
	[[Category: Morse, R J.]]		[[Category: Morse, R J.]]
	[[Category: Stroud, R M.]]		[[Category: Stroud, R M.]]
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	[[Category: Lectin-like beta sandwich]]		[[Category: Lectin-like beta sandwich]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1~~ 10:27:~~31 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 10:54:21 2009''