1i7p - Revision history

OCA at 06:24, 9 August 2023

OCA — Wed, 09 Aug 2023 06:24:08 GMT

←Older revision		Revision as of 06:24, 9 August 2023
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	<StructureSection load='1i7p' size='340' side='right'caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1i7p' size='340' side='right'caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1ndh\|1ndh]], [[1ib0\|1ib0]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [https://pdbe.org/1i7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [https://www.ebi.ac.uk/pdbsum/1i7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i7p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [https://pdbe.org/1i7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [https://www.ebi.ac.uk/pdbsum/1i7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NB5R3_RAT NB5R3_RAT]] Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.	+	[https://www.uniprot.org/uniprot/NB5R3_RAT NB5R3_RAT] Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cytochrome-b5 reductase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Barber, M J~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Bewley, M C~~]]	+	[[Category: Barber MJ]]
-	[[Category: ~~Marohnic, C C~~]]	+	[[Category: Bewley MC]]
-	[[Category: ~~Electron transport]]~~	+	[[Category: Marohnic CC]]
-	~~[[Category: Erythrocyte function]]~~	+
-	~~[[Category: Fad-binding]]~~	+
-	~~[[Category: Hemoglobinemia]]~~	+
-	~~[[Category: Nadh-binding]]~~	+
-	~~[[Category: Oxygen storage-transport complex~~]]	+

OCA at 07:37, 7 April 2021

OCA — Wed, 07 Apr 2021 07:37:00 GMT

←Older revision		Revision as of 07:37, 7 April 2021
Line 3:		Line 3:
	<StructureSection load='1i7p' size='340' side='right'caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1i7p' size='340' side='right'caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1I7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ndh\|1ndh]], [[1ib0\|1ib0]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [~~http~~://pdbe.org/1i7p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1i7p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1i7p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [https://pdbe.org/1i7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [https://www.ebi.ac.uk/pdbsum/1i7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NB5R3_RAT NB5R3_RAT]] Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.	+	[[https://www.uniprot.org/uniprot/NB5R3_RAT NB5R3_RAT]] Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:26, 3 July 2019

OCA — Wed, 03 Jul 2019 07:26:11 GMT

←Older revision		Revision as of 07:26, 3 July 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD==		==CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD==
-	<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1i7p' size='340' side='right'caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/1i7p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/1i7p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1i7p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1i7p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[NADH-cytochrome b5 reductase\|NADH-cytochrome b5 reductase]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
		+	[[Category: Large Structures]]
	[[Category: Barber, M J]]		[[Category: Barber, M J]]
	[[Category: Bewley, M C]]		[[Category: Bewley, M C]]

OCA at 10:51, 27 September 2017

OCA — Wed, 27 Sep 2017 10:51:08 GMT

←Older revision		Revision as of 10:51, 27 September 2017
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD==		==CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD==
	<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://pdbe.org/1i7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://pdbe.org/1i7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i7p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
	[[Category: Barber, M J]]		[[Category: Barber, M J]]

OCA at 14:29, 11 September 2015

OCA — Fri, 11 Sep 2015 14:29:46 GMT

←Older revision		Revision as of 14:29, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 ~~Rattus norvegicus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://pdbe.org/1i7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1i7p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Buffalo rat]]
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
-	[[Category: Rattus norvegicus]]
	[[Category: Barber, M J]]		[[Category: Barber, M J]]
	[[Category: Bewley, M C]]		[[Category: Bewley, M C]]

OCA at 17:44, 25 December 2014

OCA — Thu, 25 Dec 2014 17:44:10 GMT

←Older revision		Revision as of 17:44, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NB5R3_RAT NB5R3_RAT]] Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Barber, M J.]]	+	[[Category: Barber, M J]]
-	[[Category: Bewley, M C.]]	+	[[Category: Bewley, M C]]
-	[[Category: Marohnic, C C.]]	+	[[Category: Marohnic, C C]]
	[[Category: Electron transport]]		[[Category: Electron transport]]
	[[Category: Erythrocyte function]]		[[Category: Erythrocyte function]]

OCA at 12:36, 28 September 2014

OCA — Sun, 28 Sep 2014 12:36:06 GMT

←Older revision		Revision as of 12:36, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1i7p.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD==
		+	<StructureSection load='1i7p' size='340' side='right' caption='[[1i7p]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I7P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ndh\|1ndh]], [[1ib0\|1ib0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-b5_reductase Cytochrome-b5 reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.2.2 1.6.2.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i7p RCSB], [http://www.ebi.ac.uk/pdbsum/1i7p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/1i7p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochrome b5 reductase (cb5r) (EC 1.6.6.2) catalyzes the reduction of two molecules of cytochrome b5 using NADH as the physiological electron donor. The structure of pig cb5r at 2.4 A resolution was previously reported in the literature, but it was inconsistent with the biochemistry; for example, K83 and C245 were both implicated in the mechanism, but were not located at the active site. To address this problem, we have determined the structures of cb5r from rat at 2.0 A resolution and in a complex with NAD+ at 2.3 A resolution. We found significant differences throughout the rat structure compared to that of pig, including the locations of the lysine and cysteine residues mentioned above. To test the structural models, we made single amino acid substitutions of this lysine and showed that all substitutions produced correctly folded proteins and exhibited normal flavin behavior. However, the apparent kcat(NADH) decreased, and the apparent K(m) for NADH increased; the K(m)'s for cytochrome b5 were unchanged relative to that of the wild type. The largest effect was for the glutamate-substituted protein, which was further characterized using a charge transfer assay and found to be less efficient at NADH utilization than the wild type. These results are consistent with a role for this lysine in stabilizing the NADH-bound form of cb5r. We have concluded that the pig structure was mistraced in several regions and have reinterpreted mutants in these regions that give rise to the hereditary disease methemoglobinemia.

-	~~{{STRUCTURE_1i7p\| PDB=1i7p \| SCENE= }}~~	+	The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent.,Bewley MC, Marohnic CC, Barber MJ Biochemistry. 2001 Nov 13;40(45):13574-82. PMID:11695905<ref>PMID:11695905</ref>

-	~~===CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11695905}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1i7p]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rattus_norvegicus Rattus norvegicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011695905<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]

OCA: Protected "1i7p" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:48:11 GMT

Protected "1i7p" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:48, 21 November 2012

OCA at 15:48, 21 November 2012

OCA — Wed, 21 Nov 2012 15:48:09 GMT

←Older revision		Revision as of 15:48, 21 November 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1i7p.png\|left\|200px]]		[[Image:1i7p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1i7p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1i7p\| PDB=1i7p \| SCENE= }}		{{STRUCTURE_1i7p\| PDB=1i7p \| SCENE= }}

	===CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD===		===CRYSTAL STRUCTURE OF RAT B5R IN COMPLEX WITH FAD===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11695905}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11695905 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11695905}}		{{ABSTRACT_PUBMED_11695905}}

	==About this Structure==		==About this Structure==
-	~~1I7P~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA].	+	[[1i7p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11695905~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011695905</ref><references group="xtra"/>
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
Line 34:		Line 22:
	[[Category: Hemoglobinemia]]		[[Category: Hemoglobinemia]]
	[[Category: Nadh-binding]]		[[Category: Nadh-binding]]
-		+	[[Category: Oxygen storage-transport complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:34:21 2009''~~	+

OCA at 22:34, 17 February 2009

OCA — Tue, 17 Feb 2009 22:34:22 GMT

←Older revision		Revision as of 22:34, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1I7P is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA].	+	1I7P is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I7P OCA].

	==Reference==		==Reference==
-	~~The structure and biochemistry of NADH-dependent cytochrome b5 reductase are now consistent., Bewley MC, Marohnic CC, Barber MJ, Biochemistry. 2001 Nov 13;40(45):13574-82.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11695905 11695905]~~	+	<ref group="xtra">PMID:11695905</ref><references group="xtra"/>
	[[Category: Cytochrome-b5 reductase]]		[[Category: Cytochrome-b5 reductase]]
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Single protein]]
	[[Category: Barber, M J.]]		[[Category: Barber, M J.]]
	[[Category: Bewley, M C.]]		[[Category: Bewley, M C.]]
Line 36:		Line 35:
	[[Category: Nadh-binding]]		[[Category: Nadh-binding]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1 10~~:32:~~46 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:34:21 2009''