1i8b - Revision history

OCA at 07:45, 3 April 2024

OCA — Wed, 03 Apr 2024 07:45:11 GMT

←Older revision		Revision as of 07:45, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='1i8b' size='340' side='right'caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1i8b' size='340' side='right'caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Alfalfa Alfalfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1bi5\|1bi5]]~~, [[~~1i86~~\|~~1i86~~]]~~, [[1i88\|1i88]], [[1i89\|1i89]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [https://pdbe.org/1i8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [https://www.ebi.ac.uk/pdbsum/1i8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [https://pdbe.org/1i8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [https://www.ebi.ac.uk/pdbsum/1i8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>	+	[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8b ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8b ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Chalcone synthase (CHS) belongs to the family of type III polyketide synthases (PKS) that catalyze formation of structurally diverse polyketides. CHS synthesizes a tetraketide by sequential condensation of three acetyl anions derived from malonyl-CoA decarboxylation to a p-coumaroyl moiety attached to an active site cysteine. Gly256 resides on the surface of the CHS active site that is in direct contact with the polyketide chain derived from malonyl-CoA. Thus, position 256 serves as an ideal target to probe the link between cavity volume and polyketide chain-length determination in type III PKS. Functional examination of CHS G256A, G256V, G256L, and G256F mutants reveals altered product profiles from that of wild-type CHS. With p-coumaroyl-CoA as a starter molecule, the G256A and G256V mutants produce notably more tetraketide lactone. Further restrictions in cavity volume such as that seen in the G256L and G256F mutants yield increasing levels of the styrylpyrone bis-noryangonin from a triketide intermediate. X-ray crystallographic structures of the CHS G256A, G256V, G256L, and G256F mutants establish that these substitutions reduce the size of the active site cavity without significant alterations in the conformations of the polypeptide backbones. The side chain volume of position 256 influences both the number of condensation reactions during polyketide chain extension and the conformation of the triketide and tetraketide intermediates during the cyclization reaction. These results viewed in conjunction with the natural sequence variation of residue 256 suggest that rapid diversification of product specificity without concomitant loss of substantial catalytic activity in related CHS-like enzymes can occur by site-specific evolution of side chain volume at position 256.
-
-	Structure-guided programming of polyketide chain-length determination in chalcone synthase.,Jez JM, Bowman ME, Noel JP Biochemistry. 2001 Dec 11;40(49):14829-38. PMID:11732902<ref>PMID:11732902</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1i8b" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Alfalfa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Naringenin-chalcone synthase~~]]	+	[[Category: Medicago sativa]]
-	[[Category: Bowman~~, M E~~]]	+	[[Category: Bowman ME]]
-	[[Category: Jez~~, J M~~]]	+	[[Category: Jez JM]]
-	[[Category: Noel~~, J P]]~~	+	[[Category: Noel JP]]
-	~~[[Category: Chalcone synthase]]~~	+
-	~~[[Category: Polyketide synthase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:57, 4 August 2021

OCA — Wed, 04 Aug 2021 10:57:57 GMT

←Older revision		Revision as of 10:57, 4 August 2021
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	<StructureSection load='1i8b' size='340' side='right'caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1i8b' size='340' side='right'caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1I8B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [~~http~~://pdbe.org/1i8b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1i8b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8b ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [https://pdbe.org/1i8b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [https://www.ebi.ac.uk/pdbsum/1i8b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>	+	[[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1i8b" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1i8b" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chalcone synthase\|Chalcone synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:09, 30 October 2019

OCA — Wed, 30 Oct 2019 10:09:46 GMT

←Older revision		Revision as of 10:09, 30 October 2019
Line 1:		Line 1:

	==Chalcone synthase (G256F)==		==Chalcone synthase (G256F)==
-	<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='1i8b' size='340' side='right'caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Alfalfa]]		[[Category: Alfalfa]]
		+	[[Category: Large Structures]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]

OCA at 09:10, 10 January 2018

OCA — Wed, 10 Jan 2018 09:10:50 GMT

←Older revision		Revision as of 09:10, 10 January 2018
Line 1:		Line 1:
		+
	==Chalcone synthase (G256F)==		==Chalcone synthase (G256F)==
	<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://pdbe.org/1i8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://pdbe.org/1i8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8b ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8b_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8b_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:43, 8 February 2016

OCA — Mon, 08 Feb 2016 07:43:28 GMT

←Older revision		Revision as of 07:43, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8b ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:22, 10 September 2015

OCA — Thu, 10 Sep 2015 09:22:07 GMT

←Older revision		Revision as of 09:22, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Medicago_sativa Medicago sativa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://pdbe.org/1i8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1i8b" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Medicago sativa~~]]	+	[[Category: Alfalfa]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]

OCA at 21:43, 24 December 2014

OCA — Wed, 24 Dec 2014 21:43:43 GMT

←Older revision		Revision as of 21:43, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA]] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Medicago sativa]]		[[Category: Medicago sativa]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
-	[[Category: Bowman, M E.]]	+	[[Category: Bowman, M E]]
-	[[Category: Jez, J M.]]	+	[[Category: Jez, J M]]
-	[[Category: Noel, J P.]]	+	[[Category: Noel, J P]]
	[[Category: Chalcone synthase]]		[[Category: Chalcone synthase]]
	[[Category: Polyketide synthase]]		[[Category: Polyketide synthase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 14:11, 28 September 2014

OCA — Sun, 28 Sep 2014 14:11:22 GMT

←Older revision		Revision as of 14:11, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1i8b.~~png~~\|~~left~~\|200px]]	+	==Chalcone synthase (G256F)==
		+	<StructureSection load='1i8b' size='340' side='right' caption='[[1i8b]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8B FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bi5\|1bi5]], [[1i86\|1i86]], [[1i88\|1i88]], [[1i89\|1i89]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8b RCSB], [http://www.ebi.ac.uk/pdbsum/1i8b PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8b_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chalcone synthase (CHS) belongs to the family of type III polyketide synthases (PKS) that catalyze formation of structurally diverse polyketides. CHS synthesizes a tetraketide by sequential condensation of three acetyl anions derived from malonyl-CoA decarboxylation to a p-coumaroyl moiety attached to an active site cysteine. Gly256 resides on the surface of the CHS active site that is in direct contact with the polyketide chain derived from malonyl-CoA. Thus, position 256 serves as an ideal target to probe the link between cavity volume and polyketide chain-length determination in type III PKS. Functional examination of CHS G256A, G256V, G256L, and G256F mutants reveals altered product profiles from that of wild-type CHS. With p-coumaroyl-CoA as a starter molecule, the G256A and G256V mutants produce notably more tetraketide lactone. Further restrictions in cavity volume such as that seen in the G256L and G256F mutants yield increasing levels of the styrylpyrone bis-noryangonin from a triketide intermediate. X-ray crystallographic structures of the CHS G256A, G256V, G256L, and G256F mutants establish that these substitutions reduce the size of the active site cavity without significant alterations in the conformations of the polypeptide backbones. The side chain volume of position 256 influences both the number of condensation reactions during polyketide chain extension and the conformation of the triketide and tetraketide intermediates during the cyclization reaction. These results viewed in conjunction with the natural sequence variation of residue 256 suggest that rapid diversification of product specificity without concomitant loss of substantial catalytic activity in related CHS-like enzymes can occur by site-specific evolution of side chain volume at position 256.

-	~~{{STRUCTURE_1i8b\| PDB=1i8b \| SCENE= }}~~	+	Structure-guided programming of polyketide chain-length determination in chalcone synthase.,Jez JM, Bowman ME, Noel JP Biochemistry. 2001 Dec 11;40(49):14829-38. PMID:11732902<ref>PMID:11732902</ref>

-	~~===Chalcone synthase (G256F)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11732902}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1i8b]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Medicago_sativa Medicago sativa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011732902<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Medicago sativa]]		[[Category: Medicago sativa]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]

OCA: Protected "1i8b" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:52:04 GMT

Protected "1i8b" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:52, 21 November 2012

OCA at 15:52, 21 November 2012

OCA — Wed, 21 Nov 2012 15:52:00 GMT

←Older revision		Revision as of 15:52, 21 November 2012
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-	{{Seed}}
	[[Image:1i8b.png\|left\|200px]]		[[Image:1i8b.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1i8b", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1i8b\| PDB=1i8b \| SCENE= }}		{{STRUCTURE_1i8b\| PDB=1i8b \| SCENE= }}

-	===~~CHALCONE SYNTHASE~~ (G256F)===	+	===Chalcone synthase (G256F)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11732902}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11732902 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11732902}}		{{ABSTRACT_PUBMED_11732902}}

	==About this Structure==		==About this Structure==
-	~~1I8B~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA].	+	[[1i8b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8B OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11732902~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011732902</ref><references group="xtra"/>
	[[Category: Medicago sativa]]		[[Category: Medicago sativa]]
	[[Category: Naringenin-chalcone synthase]]		[[Category: Naringenin-chalcone synthase]]
Line 32:		Line 20:
	[[Category: Polyketide synthase]]		[[Category: Polyketide synthase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 23 20:22:42 2009''