1i8j - Revision history

OCA at 06:24, 9 August 2023

OCA — Wed, 09 Aug 2023 06:24:24 GMT

←Older revision		Revision as of 06:24, 9 August 2023
Line 3:		Line 3:
	<StructureSection load='1i8j' size='340' side='right'caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1i8j' size='340' side='right'caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [https://pdbe.org/1i8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [https://www.ebi.ac.uk/pdbsum/1i8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [https://pdbe.org/1i8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [https://www.ebi.ac.uk/pdbsum/1i8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HEM2_ECOLI HEM2_ECOLI]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.	+	[https://www.uniprot.org/uniprot/HEM2_ECOLI HEM2_ECOLI] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Porphobilinogen synthase]]~~	+	[[Category: Jaffe EK]]
-	[[Category: Jaffe~~, E K~~]]	+	[[Category: Kervinen J]]
-	[[Category: Kervinen, J]]	+	[[Category: Neier R]]
-	[[Category: Neier, R]]	+	[[Category: Stauffer F]]
-	[[Category: Stauffer, F]]	+	[[Category: Wlodawer A]]
-	[[Category: Wlodawer, A]]	+	[[Category: Zdanov A]]
-	[[Category: Zdanov, A]]	+
-	~~[[Category: 7-dioxosebacic acid]]~~	+
-	~~[[Category: Heme biosynthesis]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Magnesium~~]]	+

OCA at 10:58, 4 August 2021

OCA — Wed, 04 Aug 2021 10:58:03 GMT

←Older revision		Revision as of 10:58, 4 August 2021
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	<StructureSection load='1i8j' size='340' side='right'caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1i8j' size='340' side='right'caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1I8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [~~http~~://pdbe.org/1i8j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1i8j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [https://pdbe.org/1i8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [https://www.ebi.ac.uk/pdbsum/1i8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HEM2_ECOLI HEM2_ECOLI]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.	+	[[https://www.uniprot.org/uniprot/HEM2_ECOLI HEM2_ECOLI]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:12, 30 October 2019

OCA — Wed, 30 Oct 2019 10:12:36 GMT

←Older revision		Revision as of 10:12, 30 October 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID==		==CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID==
-	<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1i8j' size='340' side='right'caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1i8j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1i8j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Porphobilinogen synthase\|Porphobilinogen synthase]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Jaffe, E K]]		[[Category: Jaffe, E K]]

OCA at 09:13, 10 January 2018

OCA — Wed, 10 Jan 2018 09:13:34 GMT

←Older revision		Revision as of 09:13, 10 January 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID==		==CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID==
	<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://pdbe.org/1i8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://pdbe.org/1i8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1i8j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1i8j" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Porphobilinogen synthase\|Porphobilinogen synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:48, 8 February 2016

OCA — Mon, 08 Feb 2016 16:48:17 GMT

←Older revision		Revision as of 16:48, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:32, 10 September 2015

OCA — Thu, 10 Sep 2015 13:32:41 GMT

←Older revision		Revision as of 13:32, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://pdbe.org/1i8j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1i8j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Jaffe, E K]]		[[Category: Jaffe, E K]]

OCA at 00:47, 25 December 2014

OCA — Thu, 25 Dec 2014 00:47:09 GMT

←Older revision		Revision as of 00:47, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HEM2_ECOLI HEM2_ECOLI]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
-	[[Category: Jaffe, E K.]]	+	[[Category: Jaffe, E K]]
-	[[Category: Kervinen, J.]]	+	[[Category: Kervinen, J]]
-	[[Category: Neier, R.]]	+	[[Category: Neier, R]]
-	[[Category: Stauffer, F.]]	+	[[Category: Stauffer, F]]
-	[[Category: Wlodawer, A.]]	+	[[Category: Wlodawer, A]]
-	[[Category: Zdanov, A.]]	+	[[Category: Zdanov, A]]
	[[Category: 7-dioxosebacic acid]]		[[Category: 7-dioxosebacic acid]]
	[[Category: Heme biosynthesis]]		[[Category: Heme biosynthesis]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Magnesium]]		[[Category: Magnesium]]

OCA at 11:58, 28 September 2014

OCA — Sun, 28 Sep 2014 11:58:00 GMT

←Older revision		Revision as of 11:58, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1i8j.~~png\|left~~\|200px]]	+	==CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID==
		+	<StructureSection load='1i8j' size='340' side='right' caption='[[1i8j]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I8J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSB:4,7-DIOXOSEBACIC+ACID'>DSB</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i8j RCSB], [http://www.ebi.ac.uk/pdbsum/1i8j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	4,7-Dioxosebacic acid (4,7-DOSA) is an active site-directed irreversible inhibitor of porphobilinogen synthase (PBGS). PBGS catalyzes the first common step in the biosynthesis of the tetrapyrrole cofactors such as heme, vitamin B(12), and chlorophyll. 4,7-DOSA was designed as an analogue of a proposed reaction intermediate in the physiological PBGS-catalyzed condensation of two molecules of 5-aminolevulinic acid. As shown here, 4,7-DOSA exhibits time-dependent and dramatic species-specific inhibition of PBGS enzymes. IC(50) values vary from 1 microM to 2.4 mM for human, Escherichia coli, Bradyrhizobium japonicum, Pseudomonas aeruginosa, and pea enzymes. Those PBGS utilizing a catalytic Zn(2+) are more sensitive to 4,7-DOSA than those that do not. Weak inhibition of a human mutant PBGS establishes that the inactivation by 4,7-DOSA requires formation of a Schiff base to a lysine that normally forms a Schiff base intermediate to one substrate molecule. A 1.9 A resolution crystal structure of E. coli PBGS complexed with 4,7-DOSA (PDB code ) shows one dimer per asymmetric unit and reveals that the inhibitor forms two Schiff base linkages with each monomer, one to the normal Schiff base-forming Lys-246 and the other to a universally conserved "perturbing" Lys-194 (E. coli numbering). This is the first structure to show inhibitor binding at the second of two substrate-binding sites.

-	~~{{STRUCTURE_1i8j\| PDB=1i8j \| SCENE= }}~~	+	Mechanistic basis for suicide inactivation of porphobilinogen synthase by 4,7-dioxosebacic acid, an inhibitor that shows dramatic species selectivity.,Kervinen J, Jaffe EK, Stauffer F, Neier R, Wlodawer A, Zdanov A Biochemistry. 2001 Jul 27;40(28):8227-36. PMID:11444968<ref>PMID:11444968</ref>

-	~~===CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4~~,~~7-DIOXOSEBACIC ACID===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_11444968}}~~	+	==See Also==
-		+	*[[Porphobilinogen synthase\|Porphobilinogen synthase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1i8j~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:011444968<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]

OCA: Protected "1i8j" [edit=sysop:move=sysop]

OCA — Wed, 21 Nov 2012 15:55:57 GMT

Protected "1i8j" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:55, 21 November 2012

OCA at 15:55, 21 November 2012

OCA — Wed, 21 Nov 2012 15:55:53 GMT

←Older revision		Revision as of 15:55, 21 November 2012
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-	{{Seed}}
	[[Image:1i8j.png\|left\|200px]]		[[Image:1i8j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1i8j", creates the "Structure Box" on the page.
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	{{STRUCTURE_1i8j\| PDB=1i8j \| SCENE= }}		{{STRUCTURE_1i8j\| PDB=1i8j \| SCENE= }}

	===CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID===		===CRYSTAL STRUCTURE OF PORPHOBILINOGEN SYNTHASE COMPLEXED WITH THE INHIBITOR 4,7-DIOXOSEBACIC ACID===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11444968}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11444968 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_11444968}}		{{ABSTRACT_PUBMED_11444968}}

	==About this Structure==		==About this Structure==
-	~~1I8J~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA].	+	[[1i8j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8J OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11444968~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011444968</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
Line 32:		Line 20:
	[[Category: Wlodawer, A.]]		[[Category: Wlodawer, A.]]
	[[Category: Zdanov, A.]]		[[Category: Zdanov, A.]]
-	[[Category: 4,7-dioxosebacic acid]]	+	[[Category: 7-dioxosebacic acid]]
	[[Category: Heme biosynthesis]]		[[Category: Heme biosynthesis]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Magnesium]]		[[Category: Magnesium]]
-	[[Category: Zinc]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 00:33:46 2009''