1idl - Revision history

OCA at 07:46, 3 April 2024

OCA — Wed, 03 Apr 2024 07:46:35 GMT

←Older revision		Revision as of 07:46, 3 April 2024
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	==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==		==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==
-	<StructureSection load='1idl' size='340' side='right'caption='[[1idl~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1idl' size='340' side='right'caption='[[1idl]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [https://pdbe.org/1idl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [https://www.ebi.ac.uk/pdbsum/1idl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [https://pdbe.org/1idl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [https://www.ebi.ac.uk/pdbsum/1idl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~NXL1A_BUNMU NXL1A_BUNMU]~~] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) ~~nicotinic acetylcholine receptors~~. ~~Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site~~. ~~Blocks~~ the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>	+	[https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1idl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1idl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The region encompassing residues 181-98 on the alpha1 subunit of the muscle-type nicotinic acetylcholine receptor forms a major determinant for the binding of alpha-neurotoxins. We have prepared an (15)N-enriched 18-amino acid peptide corresponding to the sequence in this region to facilitate structural elucidation by multidimensional NMR. Our aim was to determine the structural basis for the high affinity, stoichiometric complex formed between this cognate peptide and alpha-bungarotoxin, a long alpha-neurotoxin. Resonances in the complex were assigned through heteronuclear and homonuclear NMR experiments, and the resulting interproton distance constraints were used to generate ensemble structures of the complex. Thr(8), Pro(10), Lys(38), Val(39), Val(40), and Pro(69) in alpha-bungarotoxin and Tyr(189), Tyr(190), Thr(191), Cys(192), Asp(195), and Thr(196) in the peptide participate in major intermolecular contacts. A comparison of the free and bound alpha-bungarotoxin structures reveals significant conformational rearrangements in flexible regions of alpha-bungarotoxin, mainly loops I, II, and the C-terminal tail. Furthermore, several of the calculated structures suggest that cation-pi interactions may be involved in binding. The root mean square deviation of the polypeptide backbone in the complex is 2.07 A. This structure provides, to date, the highest resolution description of the contacts between a prototypic alpha-neurotoxin and its cognate recognition sequence.
-
-	The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica.,Zeng H, Moise L, Grant MA, Hawrot E J Biol Chem. 2001 Jun 22;276(25):22930-40. Epub 2001 Apr 18. PMID:11312275<ref>PMID:11312275</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 28:
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Grant~~, M A~~]]	+	[[Category: Grant MA]]
-	[[Category: Hawrot, E]]	+	[[Category: Hawrot E]]
-	[[Category: Moise, L]]	+	[[Category: Moise L]]
-	[[Category: Zeng, H]]	+	[[Category: Zeng H]]
-	~~[[Category: Alpha-bungarotoxin]]~~	+
-	~~[[Category: Alpha-neurotoxin]]~~	+
-	~~[[Category: Nmr solution structure]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:59, 4 August 2021

OCA — Wed, 04 Aug 2021 10:59:46 GMT

←Older revision		Revision as of 10:59, 4 August 2021
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	<StructureSection load='1idl' size='340' side='right'caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1idl' size='340' side='right'caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1IDL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [~~http~~://pdbe.org/1idl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1idl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1idl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [https://pdbe.org/1idl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [https://www.ebi.ac.uk/pdbsum/1idl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1idl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>	+	[[https://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 10:32, 30 October 2019

OCA — Wed, 30 Oct 2019 10:32:02 GMT

←Older revision		Revision as of 10:32, 30 October 2019
Line 1:		Line 1:

	==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==		==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==
-	<StructureSection load='1idl' size='340' side='right' caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1idl' size='340' side='right'caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bungarotoxin 3D structures\|Bungarotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
		+	[[Category: Large Structures]]
	[[Category: Grant, M A]]		[[Category: Grant, M A]]
	[[Category: Hawrot, E]]		[[Category: Hawrot, E]]

OCA at 09:32, 10 January 2018

OCA — Wed, 10 Jan 2018 09:32:20 GMT

←Older revision		Revision as of 09:32, 10 January 2018
Line 1:		Line 1:
		+
	==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==		==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==
	<StructureSection load='1idl' size='340' side='right' caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1idl' size='340' side='right' caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://pdbe.org/1idl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://pdbe.org/1idl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1idl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1idl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1idl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Bungarotoxin\|Bungarotoxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 19:07, 8 February 2016

OCA — Mon, 08 Feb 2016 19:07:59 GMT

←Older revision		Revision as of 19:07, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1idl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:32, 10 September 2015

OCA — Thu, 10 Sep 2015 14:32:29 GMT

←Older revision		Revision as of 14:32, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://pdbe.org/1idl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1idl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bungarotoxin\|Bungarotoxin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:32, 25 December 2014

OCA — Thu, 25 Dec 2014 01:32:44 GMT

←Older revision		Revision as of 01:32, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>		<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
-
-	==See Also==
-	*[[Bungarotoxin\|Bungarotoxin]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
-	[[Category: Grant, M A.]]	+	[[Category: Grant, M A]]
-	[[Category: Hawrot, E.]]	+	[[Category: Hawrot, E]]
-	[[Category: Moise, L.]]	+	[[Category: Moise, L]]
-	[[Category: Zeng, H.]]	+	[[Category: Zeng, H]]
	[[Category: Alpha-bungarotoxin]]		[[Category: Alpha-bungarotoxin]]
	[[Category: Alpha-neurotoxin]]		[[Category: Alpha-neurotoxin]]
	[[Category: Nmr solution structure]]		[[Category: Nmr solution structure]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 12:57, 28 September 2014

OCA — Sun, 28 Sep 2014 12:57:34 GMT

←Older revision		Revision as of 12:57, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1idl.~~png~~\|~~left~~\|200px]]	+	==THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN==
		+	<StructureSection load='1idl' size='340' side='right' caption='[[1idl]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IDL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1idg\|1idg]], [[1idh\|1idh]], [[1idi\|1idi]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1idl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1idl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1idl RCSB], [http://www.ebi.ac.uk/pdbsum/1idl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/1idl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The region encompassing residues 181-98 on the alpha1 subunit of the muscle-type nicotinic acetylcholine receptor forms a major determinant for the binding of alpha-neurotoxins. We have prepared an (15)N-enriched 18-amino acid peptide corresponding to the sequence in this region to facilitate structural elucidation by multidimensional NMR. Our aim was to determine the structural basis for the high affinity, stoichiometric complex formed between this cognate peptide and alpha-bungarotoxin, a long alpha-neurotoxin. Resonances in the complex were assigned through heteronuclear and homonuclear NMR experiments, and the resulting interproton distance constraints were used to generate ensemble structures of the complex. Thr(8), Pro(10), Lys(38), Val(39), Val(40), and Pro(69) in alpha-bungarotoxin and Tyr(189), Tyr(190), Thr(191), Cys(192), Asp(195), and Thr(196) in the peptide participate in major intermolecular contacts. A comparison of the free and bound alpha-bungarotoxin structures reveals significant conformational rearrangements in flexible regions of alpha-bungarotoxin, mainly loops I, II, and the C-terminal tail. Furthermore, several of the calculated structures suggest that cation-pi interactions may be involved in binding. The root mean square deviation of the polypeptide backbone in the complex is 2.07 A. This structure provides, to date, the highest resolution description of the contacts between a prototypic alpha-neurotoxin and its cognate recognition sequence.

-	~~{{STRUCTURE_1idl\| PDB=1idl \| SCENE= }}~~	+	The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica.,Zeng H, Moise L, Grant MA, Hawrot E J Biol Chem. 2001 Jun 22;276(25):22930-40. Epub 2001 Apr 18. PMID:11312275<ref>PMID:11312275</ref>

-	~~===THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11312275}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1idl]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bungarus_multicinctus Bungarus multicinctus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA].~~	+

	==See Also==		==See Also==
	*[[Bungarotoxin\|Bungarotoxin]]		*[[Bungarotoxin\|Bungarotoxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011312275<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Grant, M A.]]		[[Category: Grant, M A.]]

OCA at 11:10, 20 October 2012

OCA — Sat, 20 Oct 2012 11:10:29 GMT

←Older revision		Revision as of 11:10, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1idl]] is a 1 chain structure ~~of [[Bungarotoxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA].	+	[[1idl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA].

	==See Also==		==See Also==

OCA at 04:28, 26 July 2012

OCA — Thu, 26 Jul 2012 04:28:23 GMT

←Older revision		Revision as of 04:28, 26 July 2012
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	[[Image:1idl.png\|left\|200px]]		[[Image:1idl.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1idl", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_1idl\| PDB=1idl \| SCENE= }}		{{STRUCTURE_1idl\| PDB=1idl \| SCENE= }}

	===THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN===		===THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11312275}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11312275 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_11312275}}		{{ABSTRACT_PUBMED_11312275}}

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	==See Also==		==See Also==
-	*[[Bungarotoxin]]	+	*[[Bungarotoxin\|Bungarotoxin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11312275~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011312275</ref><references group="xtra"/>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Grant, M A.]]		[[Category: Grant, M A.]]
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	[[Category: Alpha-neurotoxin]]		[[Category: Alpha-neurotoxin]]
	[[Category: Nmr solution structure]]		[[Category: Nmr solution structure]]
		+	[[Category: Toxin]]