1ie5 - Revision history

OCA at 07:46, 3 April 2024

OCA — Wed, 03 Apr 2024 07:46:41 GMT

←Older revision		Revision as of 07:46, 3 April 2024
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	==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==		==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==
-	<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [https://pdbe.org/1ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [https://pdbe.org/1ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NCAM1_CHICK NCAM1_CHICK]] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.	+	[https://www.uniprot.org/uniprot/NCAM1_CHICK NCAM1_CHICK] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ie5 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ie5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Homophilic binding of the neural cell adhesion molecule (N-CAM) mediates the calcium-independent cell-cell adhesion that is involved in neuronal development. Two hypotheses have been advanced for the mechanism of homophilic binding. Cell-based experiments have implicated each of the five extracellular immunoglobulin (Ig) domains of N-CAM in the homophilic adhesion interaction, and have predicted that the third domain (Ig III) self-associates. The alternative hypothesis is based on solution observations, which implicate a specific antiparallel interaction between the first two Ig domains (Ig I and Ig II). In order to test these hypotheses, we have determined a high-resolution solution structure of recombinant Ig III (sequence derived from chicken N-CAM) and examined the aggregation behavior of isolated Ig domains in solution. The structure shows that Ig III adopts a canonical Ig fold, in which the beta strands ABED and A'GFCC' form two beta sheets that are linked by a disulfide bond. In contrast to the demonstrated aggregation of Ig III on solid supports, we were unable to demonstrate self-association of Ig III under any of a variety of solution conditions. The structure shows that the surface of Ig III is dominated by two large acidic patches, which may explain our failure to observe self-association in solution. To evaluate the involvement of the Ig I-Ig II interaction in cell-cell adhesion, we designed a point mutation in Ig I (F19S) that proved sufficient to abrogate the Ig I-Ig II interaction seen in solution. However, the introduction of this mutation into full-length N-CAM expressed in COS-7 cells failed to affect N-CAM-mediated cell-cell adhesion. The inability to observe Ig III self-association in solution, combined with the failure of the F19S mutation to affect N-CAM-mediated cell-cell adhesion, suggests that, although solution studies can give important insights into the structures of individual domains, the interactions observed in solution between the domains may not be representative of the interactions that occur on the cell surface.
-
-	Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding?,Atkins AR, Chung J, Deechongkit S, Little EB, Edelman GM, Wright PE, Cunningham BA, Dyson HJ J Mol Biol. 2001 Aug 3;311(1):161-72. PMID:11469865<ref>PMID:11469865</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ie5" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Atkins~~, A R~~]]	+	[[Category: Atkins AR]]
-	[[Category: Chung, J]]	+	[[Category: Chung J]]
-	[[Category: Cunningham~~, B A~~]]	+	[[Category: Cunningham BA]]
-	[[Category: Deechongkit, S]]	+	[[Category: Deechongkit S]]
-	[[Category: Dyson~~, H J~~]]	+	[[Category: Dyson HJ]]
-	[[Category: Edelman~~, G M~~]]	+	[[Category: Edelman GM]]
-	[[Category: Little~~, E B~~]]	+	[[Category: Little EB]]
-	[[Category: Wright~~, P E]]~~	+	[[Category: Wright PE]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Intermediate immunoglobulin fold~~]]	+

OCA at 07:57, 23 February 2022

OCA — Wed, 23 Feb 2022 07:57:23 GMT

←Older revision		Revision as of 07:57, 23 February 2022
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	<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [https://pdbe.org/1ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [https://pdbe.org/1ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>
	</table>		</table>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chick]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Atkins, A R]]		[[Category: Atkins, A R]]

OCA at 07:40, 7 April 2021

OCA — Wed, 07 Apr 2021 07:40:56 GMT

←Older revision		Revision as of 07:40, 7 April 2021
Line 3:		Line 3:
	<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [~~http~~://pdbe.org/1ie5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [https://pdbe.org/1ie5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NCAM1_CHICK NCAM1_CHICK]] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.	+	[[https://www.uniprot.org/uniprot/NCAM1_CHICK NCAM1_CHICK]] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:23, 3 July 2019

OCA — Wed, 03 Jul 2019 07:23:52 GMT

←Older revision		Revision as of 07:23, 3 July 2019
Line 1:		Line 1:

	==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==		==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==
-	<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1ie5' size='340' side='right'caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1ie5_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1ie5_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Atkins, A R]]		[[Category: Atkins, A R]]
	[[Category: Chung, J]]		[[Category: Chung, J]]

OCA at 10:49, 27 September 2017

OCA — Wed, 27 Sep 2017 10:49:22 GMT

←Older revision		Revision as of 10:49, 27 September 2017
Line 1:		Line 1:
		+
	==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==		==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==
	<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Chick Chick]~~. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://pdbe.org/1ie5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://pdbe.org/1ie5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ie5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ie5 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 30:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Chick]]
	[[Category: Atkins, A R]]		[[Category: Atkins, A R]]
	[[Category: Chung, J]]		[[Category: Chung, J]]

OCA at 14:27, 11 September 2015

OCA — Fri, 11 Sep 2015 14:27:52 GMT

←Older revision		Revision as of 14:27, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://pdbe.org/1ie5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ie5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Atkins, A R]]		[[Category: Atkins, A R]]
	[[Category: Chung, J]]		[[Category: Chung, J]]

OCA at 17:42, 25 December 2014

OCA — Thu, 25 Dec 2014 17:42:48 GMT

←Older revision		Revision as of 17:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NCAM1_CHICK NCAM1_CHICK]] This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Atkins, A R.]]	+	[[Category: Atkins, A R]]
-	[[Category: Chung, J.]]	+	[[Category: Chung, J]]
-	[[Category: Cunningham, B A.]]	+	[[Category: Cunningham, B A]]
-	[[Category: Deechongkit, S.]]	+	[[Category: Deechongkit, S]]
-	[[Category: Dyson, H J.]]	+	[[Category: Dyson, H J]]
-	[[Category: Edelman, G M.]]	+	[[Category: Edelman, G M]]
-	[[Category: Little, E B.]]	+	[[Category: Little, E B]]
-	[[Category: Wright, P E.]]	+	[[Category: Wright, P E]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]
	[[Category: Intermediate immunoglobulin fold]]		[[Category: Intermediate immunoglobulin fold]]

OCA at 12:34, 28 September 2014

OCA — Sun, 28 Sep 2014 12:34:19 GMT

←Older revision		Revision as of 12:34, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1ie5.~~png\|left~~\|200px]]	+	==NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.==
		+	<StructureSection load='1ie5' size='340' side='right' caption='[[1ie5]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1IE5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ie5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ie5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ie5 RCSB], [http://www.ebi.ac.uk/pdbsum/1ie5 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ie/1ie5_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Homophilic binding of the neural cell adhesion molecule (N-CAM) mediates the calcium-independent cell-cell adhesion that is involved in neuronal development. Two hypotheses have been advanced for the mechanism of homophilic binding. Cell-based experiments have implicated each of the five extracellular immunoglobulin (Ig) domains of N-CAM in the homophilic adhesion interaction, and have predicted that the third domain (Ig III) self-associates. The alternative hypothesis is based on solution observations, which implicate a specific antiparallel interaction between the first two Ig domains (Ig I and Ig II). In order to test these hypotheses, we have determined a high-resolution solution structure of recombinant Ig III (sequence derived from chicken N-CAM) and examined the aggregation behavior of isolated Ig domains in solution. The structure shows that Ig III adopts a canonical Ig fold, in which the beta strands ABED and A'GFCC' form two beta sheets that are linked by a disulfide bond. In contrast to the demonstrated aggregation of Ig III on solid supports, we were unable to demonstrate self-association of Ig III under any of a variety of solution conditions. The structure shows that the surface of Ig III is dominated by two large acidic patches, which may explain our failure to observe self-association in solution. To evaluate the involvement of the Ig I-Ig II interaction in cell-cell adhesion, we designed a point mutation in Ig I (F19S) that proved sufficient to abrogate the Ig I-Ig II interaction seen in solution. However, the introduction of this mutation into full-length N-CAM expressed in COS-7 cells failed to affect N-CAM-mediated cell-cell adhesion. The inability to observe Ig III self-association in solution, combined with the failure of the F19S mutation to affect N-CAM-mediated cell-cell adhesion, suggests that, although solution studies can give important insights into the structures of individual domains, the interactions observed in solution between the domains may not be representative of the interactions that occur on the cell surface.

-	~~{{STRUCTURE_1ie5\| PDB=1ie5 \| SCENE= }}~~	+	Solution structure of the third immunoglobulin domain of the neural cell adhesion molecule N-CAM: can solution studies define the mechanism of homophilic binding?,Atkins AR, Chung J, Deechongkit S, Little EB, Edelman GM, Wright PE, Cunningham BA, Dyson HJ J Mol Biol. 2001 Aug 3;311(1):161-72. PMID:11469865<ref>PMID:11469865</ref>

-	~~===NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11469865}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ie5]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Gallus_gallus Gallus gallus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:011469865<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Atkins, A R.]]		[[Category: Atkins, A R.]]

OCA: Protected "1ie5" [edit=sysop:move=sysop]

OCA — Wed, 28 Nov 2012 15:07:38 GMT

Protected "1ie5" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:07, 28 November 2012

OCA at 15:07, 28 November 2012

OCA — Wed, 28 Nov 2012 15:07:35 GMT

←Older revision		Revision as of 15:07, 28 November 2012
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-	{{Seed}}
	[[Image:1ie5.png\|left\|200px]]		[[Image:1ie5.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ie5", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ie5\| PDB=1ie5 \| SCENE= }}		{{STRUCTURE_1ie5\| PDB=1ie5 \| SCENE= }}

	===NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.===		===NMR STRUCTURE OF THE THIRD IMMUNOGLOBULIN DOMAIN FROM THE NEURAL CELL ADHESION MOLECULE.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11469865}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11469865 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11469865}}		{{ABSTRACT_PUBMED_11469865}}

	==About this Structure==		==About this Structure==
-	~~1IE5~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA].	+	[[1ie5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IE5 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11469865~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011469865</ref><references group="xtra"/>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Atkins, A R.]]		[[Category: Atkins, A R.]]
Line 33:		Line 21:
	[[Category: Little, E B.]]		[[Category: Little, E B.]]
	[[Category: Wright, P E.]]		[[Category: Wright, P E.]]
		+	[[Category: Cell adhesion]]
	[[Category: Intermediate immunoglobulin fold]]		[[Category: Intermediate immunoglobulin fold]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:32:51 2009''