1j0j - Revision history

OCA at 23:40, 27 December 2023

OCA — Wed, 27 Dec 2023 23:40:11 GMT

←Older revision		Revision as of 23:40, 27 December 2023
Line 3:		Line 3:
	<StructureSection load='1j0j' size='340' side='right'caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1j0j' size='340' side='right'caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_12980 Atcc 12980~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900010:alpha-maltotetraose'>PRD_900010</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [https://pdbe.org/1j0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [https://www.ebi.ac.uk/pdbsum/1j0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [https://pdbe.org/1j0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [https://www.ebi.ac.uk/pdbsum/1j0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NEPU_GEOSE NEPU_GEOSE]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.	+	[https://www.uniprot.org/uniprot/NEPU_GEOSE NEPU_GEOSE] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 12980~~]]	+	[[Category: Geobacillus stearothermophilus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Neopullulanase]]~~	+	[[Category: Hondoh H]]
-	[[Category: Hondoh, H]]	+	[[Category: Kuriki T]]
-	[[Category: Kuriki, T]]	+	[[Category: Matsuura Y]]
-	[[Category: Matsuura, Y]]	+
-	~~[[Category: Beta-alpha-barrel]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:15, 14 April 2021

OCA — Wed, 14 Apr 2021 07:15:27 GMT

←Older revision		Revision as of 07:15, 14 April 2021
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	<StructureSection load='1j0j' size='340' side='right'caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1j0j' size='340' side='right'caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1J0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [~~http~~://pdbe.org/1j0j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1j0j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [https://pdbe.org/1j0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [https://www.ebi.ac.uk/pdbsum/1j0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NEPU_GEOSE NEPU_GEOSE]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.	+	[[https://www.uniprot.org/uniprot/NEPU_GEOSE NEPU_GEOSE]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:50, 10 July 2019

OCA — Wed, 10 Jul 2019 06:50:43 GMT

←Older revision		Revision as of 06:50, 10 July 2019
Line 1:		Line 1:

	==Crystal structure of neopullulanase E357Q complex with maltotetraose==		==Crystal structure of neopullulanase E357Q complex with maltotetraose==
-	<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1j0j' size='340' side='right'caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/1j0j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/1j0j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Amylase\|Amylase]]	+	*[[Amylase 3D structures\|Amylase 3D structures]]
-	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]	+
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 12980]]		[[Category: Atcc 12980]]
		+	[[Category: Large Structures]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	[[Category: Hondoh, H]]		[[Category: Hondoh, H]]

OCA at 09:41, 4 October 2017

OCA — Wed, 04 Oct 2017 09:41:26 GMT

←Older revision		Revision as of 09:41, 4 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of neopullulanase E357Q complex with maltotetraose==		==Crystal structure of neopullulanase E357Q complex with maltotetraose==
	<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://pdbe.org/1j0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://pdbe.org/1j0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j0j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:34, 11 September 2015

OCA — Fri, 11 Sep 2015 23:34:26 GMT

←Older revision		Revision as of 23:34, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Geobacillus_stearothermophilus Geobacillus stearothermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://pdbe.org/1j0j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~NEPU_BACST NEPU_BACST~~]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.	+	[[http://www.uniprot.org/uniprot/NEPU_GEOSE NEPU_GEOSE]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1j0j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Geobacillus stearothermophilus~~]]	+	[[Category: Atcc 12980]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
	[[Category: Hondoh, H]]		[[Category: Hondoh, H]]

OCA at 20:53, 25 December 2014

OCA — Thu, 25 Dec 2014 20:53:49 GMT

←Older revision		Revision as of 20:53, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NEPU_BACST NEPU_BACST]] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	==See Also==		==See Also==
	*[[Amylase\|Amylase]]		*[[Amylase\|Amylase]]
		+	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Geobacillus stearothermophilus]]		[[Category: Geobacillus stearothermophilus]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
-	[[Category: Hondoh, H.]]	+	[[Category: Hondoh, H]]
-	[[Category: Kuriki, T.]]	+	[[Category: Kuriki, T]]
-	[[Category: Matsuura, Y.]]	+	[[Category: Matsuura, Y]]
	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 11:37, 28 September 2014

OCA — Sun, 28 Sep 2014 11:37:20 GMT

←Older revision		Revision as of 11:37, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1j0j.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of neopullulanase E357Q complex with maltotetraose==
		+	<StructureSection load='1j0j' size='340' side='right' caption='[[1j0j]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1j0h\|1j0h]], [[1j0i\|1j0i]], [[1j0k\|1j0k]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j0j RCSB], [http://www.ebi.ac.uk/pdbsum/1j0j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j0/1j0j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu357-->Gln was used for complex crystallization. The structures were refined at accuracies with r.m.s. deviations of bond lengths and bond angles ranging from 0.005A to 0.008A and 1.3 degrees to 1.4 degrees, respectively. The active enzyme forms a dimer in the crystalline state and in solution. The monomer enzyme is composed of four domains, N, A, B and C, and has a (beta/alpha)(8)-barrel in domain A. The active site lies between domain A and domain N from the other monomer. The results show that dimer formation makes the active-site cleft narrower than those of ordinary alpha-amylases, which may contribute to the unique substrate specificity of this enzyme toward both alpha-1,4 and alpha-1,6-glucosidic linkages. This specificity may be influenced by the subsite structure. Only subsites -1 and -2 are commonly occupied by the product and substrates, suggesting that equivocal recognition occurs at the other subsites, which contributes to the wide substrate specificity of this enzyme.

-	~~{{STRUCTURE_1j0j\| PDB=1j0j \| SCENE= }}~~	+	Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase.,Hondoh H, Kuriki T, Matsuura Y J Mol Biol. 2003 Feb 7;326(1):177-88. PMID:12547200<ref>PMID:12547200</ref>

-	~~===Crystal structure~~ of ~~neopullulanase E357Q complex with maltotetraose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_12547200}}~~	+	==See Also==
-		+	*[[Amylase\|Amylase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1j0j~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:012547200<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Geobacillus stearothermophilus]]		[[Category: Geobacillus stearothermophilus]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]

OCA: Protected "1j0j" [edit=sysop:move=sysop]

OCA — Wed, 28 Nov 2012 14:50:56 GMT

Protected "1j0j" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:50, 28 November 2012

OCA at 14:50, 28 November 2012

OCA — Wed, 28 Nov 2012 14:50:51 GMT

←Older revision		Revision as of 14:50, 28 November 2012
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-	{{Seed}}
	[[Image:1j0j.png\|left\|200px]]		[[Image:1j0j.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1j0j", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1j0j\| PDB=1j0j \| SCENE= }}		{{STRUCTURE_1j0j\| PDB=1j0j \| SCENE= }}

	===Crystal structure of neopullulanase E357Q complex with maltotetraose===		===Crystal structure of neopullulanase E357Q complex with maltotetraose===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12547200}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12547200 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12547200}}		{{ABSTRACT_PUBMED_12547200}}

	==About this Structure==		==About this Structure==
-	~~1J0J~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA].	+	[[1j0j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12547200~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012547200</ref><references group="xtra"/>
	[[Category: Geobacillus stearothermophilus]]		[[Category: Geobacillus stearothermophilus]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
Line 30:		Line 18:
	[[Category: Matsuura, Y.]]		[[Category: Matsuura, Y.]]
	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]
-		+	[[Category: Hydrolase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 06:54:19 2009''~~	+

OCA at 04:54, 18 February 2009

OCA — Wed, 18 Feb 2009 04:54:21 GMT

←Older revision		Revision as of 04:54, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1J0J is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA].	+	1J0J is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0J OCA].

	==Reference==		==Reference==
-	~~Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase., Hondoh H, Kuriki T, Matsuura Y, J Mol Biol. 2003 Feb 7;326(1):177-88.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12547200 12547200]~~	+	<ref group="xtra">PMID:12547200</ref><references group="xtra"/>
	[[Category: Geobacillus stearothermophilus]]		[[Category: Geobacillus stearothermophilus]]
	[[Category: Neopullulanase]]		[[Category: Neopullulanase]]
-	[[Category: Single protein]]
	[[Category: Hondoh, H.]]		[[Category: Hondoh, H.]]
	[[Category: Kuriki, T.]]		[[Category: Kuriki, T.]]
Line 32:		Line 31:
	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1 14:~~18:~~53 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:54:19 2009''