1j15 - Revision history

OCA at 23:40, 27 December 2023

OCA — Wed, 27 Dec 2023 23:40:25 GMT

←Older revision		Revision as of 23:40, 27 December 2023
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	<StructureSection load='1j15' size='340' side='right'caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1j15' size='340' side='right'caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [https://pdbe.org/1j15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [https://www.ebi.ac.uk/pdbsum/1j15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j15 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [https://pdbe.org/1j15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [https://www.ebi.ac.uk/pdbsum/1j15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j15 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRY2_RAT TRY2_RAT]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Trypsin~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Stubbs~~, M T]]~~	+	[[Category: Stubbs MT]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Serine proteinase~~]]	+

OCA at 11:09, 4 August 2021

OCA — Wed, 04 Aug 2021 11:09:02 GMT

←Older revision		Revision as of 11:09, 4 August 2021
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	<StructureSection load='1j15' size='340' side='right'caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1j15' size='340' side='right'caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1J15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [~~http~~://pdbe.org/1j15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1j15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1j15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [https://pdbe.org/1j15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [https://www.ebi.ac.uk/pdbsum/1j15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j15 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:54, 30 October 2019

OCA — Wed, 30 Oct 2019 09:54:43 GMT

←Older revision		Revision as of 09:54, 30 October 2019
Line 1:		Line 1:

	==BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99/175/190RT==		==BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99/175/190RT==
-	<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1j15' size='340' side='right'caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 1j15" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1j15" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>
Line 33:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Stubbs, M T]]		[[Category: Stubbs, M T]]

OCA at 08:40, 17 January 2018

OCA — Wed, 17 Jan 2018 08:40:29 GMT

←Older revision		Revision as of 08:40, 17 January 2018
Line 1:		Line 1:
		+
	==BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99/175/190RT==		==BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99/175/190RT==
	<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://pdbe.org/1j15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://pdbe.org/1j15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1j15 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/1j15_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/1j15_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</div>		</div>
	<div class="pdbe-citations 1j15" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1j15" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Trypsin\|Trypsin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:15, 9 February 2016

OCA — Tue, 09 Feb 2016 07:15:57 GMT

←Older revision		Revision as of 07:15, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j15 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:52, 10 September 2015

OCA — Thu, 10 Sep 2015 19:52:41 GMT

←Older revision		Revision as of 19:52, 10 September 2015
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	<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://pdbe.org/1j15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1j15" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Buffalo rat]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Stubbs, M T]]		[[Category: Stubbs, M T]]

OCA at 12:04, 2 January 2015

OCA — Fri, 02 Jan 2015 12:04:04 GMT

←Older revision		Revision as of 12:04, 2 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Stubbs, M T.]]	+	[[Category: Stubbs, M T]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]
	[[Category: Serine proteinase]]		[[Category: Serine proteinase]]

OCA at 12:24, 28 September 2014

OCA — Sun, 28 Sep 2014 12:24:19 GMT

←Older revision		Revision as of 12:24, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1j15.~~png~~\|~~left~~\|200px]]	+	==BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99/175/190RT==
		+	<StructureSection load='1j15' size='340' side='right' caption='[[1j15]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J15 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ql7\|1ql7]], [[1ql8\|1ql8]], [[1ql9\|1ql9]], [[1j14\|1j14]], [[1j16\|1j16]], [[1j17\|1j17]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1j15 RCSB], [http://www.ebi.ac.uk/pdbsum/1j15 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/1j15_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In order to investigate issues of selectivity and specificity in protein-ligand interactions, we have undertaken the reconstruction of the binding pocket of human factor Xa in the structurally related rat trypsin by site-directed mutagenesis. Three sequential regions (the "99"-, the "175"- and the "190"- loops) were selected as representing the major structural differences between the ligand binding sites of the two enzymes. Wild-type rat trypsin and variants X99rT and X(99/175/190)rT were expressed in yeast, and analysed for their interaction with factor Xa and trypsin inhibitors. For most of the inhibitors studied, progressive loop replacement at the trypsin surface resulted in inhibitory profiles akin to factor Xa. Crystals of the variants were obtained in the presence of benzamidine (3), and could be soaked with the highly specific factor Xa inhibitor (1). Binding of the latter to X99rT results in a series of structural adaptations to the ligand, including the establishment of an "aromatic box" characteristic of factor Xa. In X(99/175/190)rT, introduction of the 175-loop results in a surprising re-orientation of the "intermediate helix", otherwise common to trypsin and factor Xa. The re-orientation is accompanied by an isomerisation of the Cys168-Cys182 disulphide bond, and burial of the critical Phe174 side-chain. In the presence of (1), a major re-organisation of the binding site takes place to yield a geometry identical to that of factor Xa. In all, binding of (1) to trypsin and its variants results in significant structural rearrangements, inducing a binding surface strongly reminiscent of factor Xa, against which the inhibitor was optimised. The structural data reveal a plasticity of the intermediate helix, which has been implicated in the functional cofactor dependency of many trypsin-like serine proteinases. This approach of grafting loops onto scaffolds of known related structures may serve to bridge the gap between structural genomics and drug design.

-	~~{{STRUCTURE_1j15\| PDB=1j15 \| SCENE= }}~~	+	Reconstructing the binding site of factor Xa in trypsin reveals ligand-induced structural plasticity.,Reyda S, Sohn C, Klebe G, Rall K, Ullmann D, Jakubke HD, Stubbs MT J Mol Biol. 2003 Jan 31;325(5):963-77. PMID:12527302<ref>PMID:12527302</ref>

-	~~===BENZAMIDINE IN COMPLEX WITH RAT TRYPSIN MUTANT X99~~/~~175/190RT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12527302}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1j15]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rattus_norvegicus Rattus norvegicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012527302<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 13:04, 20 October 2012

OCA — Sat, 20 Oct 2012 13:04:57 GMT

←Older revision		Revision as of 13:04, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1j15]] is a 1 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA].	+	[[1j15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J15 OCA].

	==See Also==		==See Also==

OCA: Protected "1j15" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 10:51:05 GMT

Protected "1j15" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:51, 26 July 2012