1jyz - Revision history

OCA: Redirecting to 4v44

OCA — Wed, 10 Dec 2014 14:57:17 GMT

Redirecting to 4v44

←Older revision		Revision as of 14:57, 10 December 2014
Line 1:		Line 1:
-	~~==E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.==~~	+	#REDIRECT [[4v44]] This PDB entry is obsolete and replaced by 4v44
-	~~<StructureSection load='1jyz' size='340' side='right' caption='~~[[~~1jyz]], [[Resolution\|resolution]] 2.70Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[1jyz~~]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JYZ FirstGlance]. <br>	+
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2FL:2-FLUORO-2-DEOXY-LACTOSE'>2FL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+
-	~~<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>~~	+
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dp0\|1dp0]], [[1f49\|1f49]], [[1jyn\|1jyn]], [[1jyv\|1jyv]], [[1jyw\|1jyw]], [[1jyx\|1jyx]], [[1jyy\|1jyy]], [[1jz0\|1jz0]], [[1jz1\|1jz1]], [[1jz2\|1jz2]], [[1jz3\|1jz3]], [[1jz4\|1jz4]], [[1jz5\|1jz5]], [[1jz6\|1jz6]], [[1jz7\|1jz7]], [[1jz8\|1jz8]]</td></tr>	+
-	~~<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jyz RCSB], [http://www.ebi.ac.uk/pdbsum/1jyz PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~== Evolutionary Conservation ==~~	+
-	~~[[Image:Consurf_key_small.gif\|200px\|right]]~~	+
-	~~Check<jmol>~~	+
-	~~<jmolCheckbox>~~	+
-	~~<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jy/1jyz_consurf.spt"</scriptWhenChecked>~~	+
-	~~<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>~~	+
-	~~<text>to colour the structure by Evolutionary Conservation</text>~~	+
-	~~</jmolCheckbox>~~	+
-	~~</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method~~ and ~~the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].~~	+
-	~~<div style="clear:both"></div>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The structures of a series of complexes designed to mimic intermediates along the reaction coordinate for beta-galactosidase are presented. These complexes clarify and enhance previous proposals regarding the catalytic mechanism. The nucleophile, Glu537, is seen to covalently bind to the galactosyl moiety. Of the two potential acids, Mg(2+) and Glu461, the latter is in better position to directly assist in leaving group departure, suggesting that the metal ion acts in a secondary role. A sodium ion plays a part in substrate binding by directly ligating the galactosyl 6-hydroxyl. The proposed reaction coordinate involves the movement of the galactosyl moiety deep into the active site pocket. For those ligands that do bind deeply there is an associated conformational change in which residues within loop 794-804 move up to 10 A closer to the site of binding. In some cases this can be inhibited by the binding of additional ligands. The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation.	+
-		+
-	A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.,Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:11732897<ref>PMID:11732897</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-		+
-	~~==See Also==~~	+
-	*[[Galactosidase\|Galactosidase]]	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Beta-galactosidase]]~~	+
-	~~[[Category: Escherichia coli]]~~	+
-	~~[[Category: Juers, D H.]]~~	+
-	~~[[Category: Matthews, B W.]]~~	+
-	~~[[Category: McCarter, J D.]]~~	+
-	~~[[Category: Withers, S G.]]~~	+
-	~~[[Category: Beta supersandwich]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Immunoglobulin]]~~	+
-	~~[[Category: Jelly-roll barrel]]~~	+

OCA at 10:58, 28 September 2014

OCA — Sun, 28 Sep 2014 10:58:13 GMT

←Older revision		Revision as of 10:58, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1jyz.~~png~~\|~~left~~\|200px]]	+	==E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.==
		+	<StructureSection load='1jyz' size='340' side='right' caption='[[1jyz]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1jyz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JYZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2FL:2-FLUORO-2-DEOXY-LACTOSE'>2FL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1dp0\|1dp0]], [[1f49\|1f49]], [[1jyn\|1jyn]], [[1jyv\|1jyv]], [[1jyw\|1jyw]], [[1jyx\|1jyx]], [[1jyy\|1jyy]], [[1jz0\|1jz0]], [[1jz1\|1jz1]], [[1jz2\|1jz2]], [[1jz3\|1jz3]], [[1jz4\|1jz4]], [[1jz5\|1jz5]], [[1jz6\|1jz6]], [[1jz7\|1jz7]], [[1jz8\|1jz8]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jyz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1jyz RCSB], [http://www.ebi.ac.uk/pdbsum/1jyz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jy/1jyz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structures of a series of complexes designed to mimic intermediates along the reaction coordinate for beta-galactosidase are presented. These complexes clarify and enhance previous proposals regarding the catalytic mechanism. The nucleophile, Glu537, is seen to covalently bind to the galactosyl moiety. Of the two potential acids, Mg(2+) and Glu461, the latter is in better position to directly assist in leaving group departure, suggesting that the metal ion acts in a secondary role. A sodium ion plays a part in substrate binding by directly ligating the galactosyl 6-hydroxyl. The proposed reaction coordinate involves the movement of the galactosyl moiety deep into the active site pocket. For those ligands that do bind deeply there is an associated conformational change in which residues within loop 794-804 move up to 10 A closer to the site of binding. In some cases this can be inhibited by the binding of additional ligands. The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation.

-	~~{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}~~	+	A structural view of the action of Escherichia coli (lacZ) beta-galactosidase.,Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:11732897<ref>PMID:11732897</ref>

-	~~===E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P~~, ~~see REMARK 400.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11732897}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1jyz]] is~~ a ~~8 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].~~	+

	==See Also==		==See Also==
	*[[Galactosidase\|Galactosidase]]		*[[Galactosidase\|Galactosidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011732897<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA at 06:07, 20 October 2012

OCA — Sat, 20 Oct 2012 06:07:57 GMT

←Older revision		Revision as of 06:07, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1jyz]] is a 8 chain structure ~~of [[Galactosidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].	+	[[1jyz]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].

	==See Also==		==See Also==

OCA: Protected "1jyz" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 12:13:19 GMT

Protected "1jyz" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:13, 25 July 2012

OCA at 12:13, 25 July 2012

OCA — Wed, 25 Jul 2012 12:13:18 GMT

←Older revision		Revision as of 12:13, 25 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1jyz.png\|left\|200px]]		[[Image:1jyz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1jyz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}		{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}

	===E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.===		===E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11732897}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11732897 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11732897}}		{{ABSTRACT_PUBMED_11732897}}

	==About this Structure==		==About this Structure==
-	~~1JYZ~~ is a 8 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].	+	[[1jyz]] is a 8 chain structure of [[Galactosidase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].
		+
		+	==See Also==
		+	*[[Galactosidase\|Galactosidase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11732897~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011732897</ref><references group="xtra"/>
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 31:		Line 22:
	[[Category: Withers, S G.]]		[[Category: Withers, S G.]]
	[[Category: Beta supersandwich]]		[[Category: Beta supersandwich]]
		+	[[Category: Hydrolase]]
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Jelly-roll barrel]]		[[Category: Jelly-roll barrel]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:37:55 2009''

OCA at 10:37, 16 February 2009

OCA — Mon, 16 Feb 2009 10:37:56 GMT

←Older revision		Revision as of 10:37, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1JYZ is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].	+	1JYZ is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].

	==Reference==		==Reference==
-	A structural view of the action of Escherichia coli (lacZ) beta-galactosidase., Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW, Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/11732897 11732897]~~	+	<ref group="xtra">PMID:11732897</ref><references group="xtra"/>
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Juers, D H.]]		[[Category: Juers, D H.]]
	[[Category: Matthews, B W.]]		[[Category: Matthews, B W.]]
Line 35:		Line 34:
	[[Category: Jelly-roll barrel]]		[[Category: Jelly-roll barrel]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 1 21~~:09:~~41 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 12:37:55 2009''

OCA at 18:09, 1 July 2008

OCA — Tue, 01 Jul 2008 18:09:43 GMT

←Older revision		Revision as of 18:09, 1 July 2008
Line 1:		Line 1:
-	[[Image:1jyz.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1jyz.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}		{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}

-	~~'''~~E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.~~'''~~	+	===E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.===


-	~~==Overview==~~	+	<!--
-	The structures of a series of complexes designed to mimic intermediates along the reaction coordinate for beta-galactosidase are presented. These complexes clarify and enhance previous proposals regarding the catalytic mechanism. The nucleophile, ~~Glu537~~, ~~is seen to covalently bind~~ to the ~~galactosyl moiety~~. ~~Of the two potential acids, Mg(2+~~) ~~and Glu461~~, ~~the latter~~ is ~~in better position to directly assist in leaving group departure, suggesting that~~ the ~~metal ion acts in a secondary role~~. ~~A sodium ion plays a part in substrate binding by directly ligating the galactosyl 6~~-hydroxyl. The proposed reaction coordinate involves the movement of the galactosyl moiety deep into the active site pocket. For those ligands that do bind deeply there is an associated conformational change in which residues within loop 794-804 move up to 10 A closer to the site of binding. In some cases this can be inhibited by the binding of additional ligands. The resulting restricted access to the intermediate helps to explain why allolactose, the natural inducer for the lac operon, is the preferred product of transglycosylation.	+	The line below this paragraph, {{ABSTRACT_PUBMED_11732897}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 11732897 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_11732897}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Immunoglobulin]]		[[Category: Immunoglobulin]]
	[[Category: Jelly-roll barrel]]		[[Category: Jelly-roll barrel]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 22~~:05:49 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:09:41 2008''

OCA at 19:05, 2 May 2008

OCA — Fri, 02 May 2008 19:05:50 GMT

←Older revision		Revision as of 19:05, 2 May 2008
Line 1:		Line 1:
	[[Image:1jyz.gif\|left\|200px]]		[[Image:1jyz.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 1jyz \|SIZE=350\|CAPTION=~~ <~~scene name='initialview01'>1jyz</scene>, resolution 2.70Å~~	+	The line below this paragraph, containing "STRUCTURE_1jyz", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <scene name='pdbligand=2FL:2~~-~~FLUORO~~-~~2-DEOXY-LACTOSE'>2FL</scene>~~, ~~<scene name='pdbligand=CME:S~~,S-(~~2-HYDROXYETHYL~~)~~THIOCYSTEINE'>CME</~~scene>, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Beta~~-~~galactosidase Beta~~-~~galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span~~>	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~= ~~lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])~~	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_1jyz\| PDB=1jyz \| SCENE= }}
-	\|RELATEDENTRY=[[1dp0\|1DP0]], [[1f49\|1F49]], [[1jyn\|1JYN]], [[1jyv\|1JYV]], [[1jyw\|1JYW]], [[1jyx\|1JYX]], [[1jyy\|1JYY]], [[1jz0\|1JZ0]], [[1jz1\|1JZ1]], [[1jz2\|1JZ2]], [[1jz3\|1JZ3]], [[1jz4\|1JZ4]], [[1jz5\|1JZ5]], [[1jz6\|1JZ6]], [[1jz7\|1JZ7]], [[1jz8\|1JZ8]]	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jyz OCA], [http://www.ebi.ac.uk/pdbsum/1jyz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jyz RCSB]</span>	+
-	}}	+

	'''E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.'''		'''E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.'''
Line 30:		Line 27:
	[[Category: McCarter, J D.]]		[[Category: McCarter, J D.]]
	[[Category: Withers, S G.]]		[[Category: Withers, S G.]]
-	[[Category: ~~beta~~ supersandwich]]	+	[[Category: Beta supersandwich]]
-	[[Category: ~~immunoglobulin~~]]	+	[[Category: Immunoglobulin]]
-	[[Category: ~~jelly~~-roll barrel]]	+	[[Category: Jelly-roll barrel]]
-	~~[[Category: tim barrel (alpha/beta barrel)]]~~	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:05:49 2008''
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Mar 30 21~~:41:00 2008''	+

OCA at 18:41, 30 March 2008

OCA — Sun, 30 Mar 2008 18:41:01 GMT

←Older revision		Revision as of 18:41, 30 March 2008
Line 4:		Line 4:
	\|PDB= 1jyz \|SIZE=350\|CAPTION= <scene name='initialview01'>1jyz</scene>, resolution 2.70Å		\|PDB= 1jyz \|SIZE=350\|CAPTION= <scene name='initialview01'>1jyz</scene>, resolution 2.70Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=2FL:2-FLUORO-2-DEOXY-LACTOSE'>2FL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> ~~and~~ <scene name='pdbligand=NA:SODIUM ION'>NA</scene>	+	\|LIGAND= <scene name='pdbligand=2FL:2-FLUORO-2-DEOXY-LACTOSE'>2FL</scene>, <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span>
	\|GENE= lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])		\|GENE= lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
		+	\|DOMAIN=
		+	\|RELATEDENTRY=[[1dp0\|1DP0]], [[1f49\|1F49]], [[1jyn\|1JYN]], [[1jyv\|1JYV]], [[1jyw\|1JYW]], [[1jyx\|1JYX]], [[1jyy\|1JYY]], [[1jz0\|1JZ0]], [[1jz1\|1JZ1]], [[1jz2\|1JZ2]], [[1jz3\|1JZ3]], [[1jz4\|1JZ4]], [[1jz5\|1JZ5]], [[1jz6\|1JZ6]], [[1jz7\|1JZ7]], [[1jz8\|1JZ8]]
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jyz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jyz OCA], [http://www.ebi.ac.uk/pdbsum/1jyz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jyz RCSB]</span>
	}}		}}

Line 27:		Line 30:
	[[Category: McCarter, J D.]]		[[Category: McCarter, J D.]]
	[[Category: Withers, S G.]]		[[Category: Withers, S G.]]
-	[[Category: 2FL]]
-	[[Category: MG]]
-	[[Category: NA]]
	[[Category: beta supersandwich]]		[[Category: beta supersandwich]]
	[[Category: immunoglobulin]]		[[Category: immunoglobulin]]
Line 35:		Line 35:
	[[Category: tim barrel (alpha/beta barrel)]]		[[Category: tim barrel (alpha/beta barrel)]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 12~~:10:24 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:41:00 2008''

OCA at 10:10, 20 March 2008

OCA — Thu, 20 Mar 2008 10:10:24 GMT

←Older revision		Revision as of 10:10, 20 March 2008
Line 1:		Line 1:
-	[[Image:1jyz.gif\|left\|200px]]~~<br /><applet load~~="1jyz~~" size~~="350~~" color~~=~~"white" frame~~=~~"true" align="right" spinBox="true"~~	+	[[Image:1jyz.gif\|left\|200px]]
-	~~caption="~~1jyz, resolution 2.70Å" />	+
-	'''E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.'''~~<br />~~	+	{{Structure
		+	\|PDB= 1jyz \|SIZE=350\|CAPTION= <scene name='initialview01'>1jyz</scene>, resolution 2.70Å
		+	\|SITE=
		+	\|LIGAND= <scene name='pdbligand=2FL:2-FLUORO-2-DEOXY-LACTOSE'>2FL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
		+	\|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23]
		+	\|GENE= lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
		+	}}
		+
		+	'''E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH 2-F-LACTOSE. Chains I-P, see REMARK 400.'''
		+

	==Overview==		==Overview==
Line 7:		Line 16:

	==About this Structure==		==About this Structure==
-	1JYZ is a [~~http://en.wikipedia.org/wiki/Single_protein~~ Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] ~~with <scene name='pdbligand=2FL:'>2FL</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en~~.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].	+	1JYZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JYZ OCA].

	==Reference==		==Reference==
-	A structural view of the action of Escherichia coli (lacZ) beta-galactosidase., Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW, Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~11732897 11732897]	+	A structural view of the action of Escherichia coli (lacZ) beta-galactosidase., Juers DH, Heightman TD, Vasella A, McCarter JD, Mackenzie L, Withers SG, Matthews BW, Biochemistry. 2001 Dec 11;40(49):14781-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11732897 11732897]
	[[Category: Beta-galactosidase]]		[[Category: Beta-galactosidase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 26:		Line 35:
	[[Category: tim barrel (alpha/beta barrel)]]		[[Category: tim barrel (alpha/beta barrel)]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21 13~~:28:18 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:10:24 2008''