1k0h - Revision history

OCA at 18:12, 29 May 2024

OCA — Wed, 29 May 2024 18:12:34 GMT

←Older revision		Revision as of 18:12, 29 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0h ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacteriophage lambda FII protein (gpFII) is a 117 residue structural protein found in the phage particle that is required for the joining of phage heads and tails at the last step of morphogenesis. We have performed biophysical experiments to show that gpFII is stable, monomeric, and reversibly folded. We have also determined the atomic resolution structure of gpFII using NMR spectroscopy. gpFII is shown to possess a novel fold consisting of seven beta-strands and a short alpha-helix. It also displays two large unstructured regions at the N terminus (residues 1-24) and in a large loop near the middle of the protein (residues 46-62). We speculate that these unstructured regions become structured when gpFII assembles into the phage particle, and that these conformational changes play an important role in regulating the assembly pathway. Alignment of the gpFII sequence with those of homologues from other lambdoid phages has allowed us to putatively identify distinct surfaces on the gpFII structure that mediate binding to the phage head and tail.
		+
		+	The solution structure of the bacteriophage lambda head-tail joining protein, gpFII.,Maxwell KL, Yee AA, Arrowsmith CH, Gold M, Davidson AR J Mol Biol. 2002 May 17;318(5):1395-404. PMID:12083526<ref>PMID:12083526</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1k0h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:56, 3 April 2024

OCA — Wed, 03 Apr 2024 07:56:21 GMT

←Older revision		Revision as of 07:56, 3 April 2024
Line 1:		Line 1:

	==Solution structure of bacteriophage lambda gpFII==		==Solution structure of bacteriophage lambda gpFII==
-	<StructureSection load='1k0h' size='340' side='right'caption='[[1k0h~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='1k0h' size='340' side='right'caption='[[1k0h]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacteriophage_lambda Bacteriophage lambda~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>FII ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10710 Bacteriophage lambda])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [https://pdbe.org/1k0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [https://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [https://pdbe.org/1k0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [https://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>	+	[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0h ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The bacteriophage lambda FII protein (gpFII) is a 117 residue structural protein found in the phage particle that is required for the joining of phage heads and tails at the last step of morphogenesis. We have performed biophysical experiments to show that gpFII is stable, monomeric, and reversibly folded. We have also determined the atomic resolution structure of gpFII using NMR spectroscopy. gpFII is shown to possess a novel fold consisting of seven beta-strands and a short alpha-helix. It also displays two large unstructured regions at the N terminus (residues 1-24) and in a large loop near the middle of the protein (residues 46-62). We speculate that these unstructured regions become structured when gpFII assembles into the phage particle, and that these conformational changes play an important role in regulating the assembly pathway. Alignment of the gpFII sequence with those of homologues from other lambdoid phages has allowed us to putatively identify distinct surfaces on the gpFII structure that mediate binding to the phage head and tail.
-
-	The solution structure of the bacteriophage lambda head-tail joining protein, gpFII.,Maxwell KL, Yee AA, Arrowsmith CH, Gold M, Davidson AR J Mol Biol. 2002 May 17;318(5):1395-404. PMID:12083526<ref>PMID:12083526</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1k0h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteriophage lambda~~]]	+	[[Category: Escherichia virus Lambda]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arrowsmith~~, C H~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: Davidson~~, A R~~]]	+	[[Category: Davidson AR]]
-	[[Category: Gold, M]]	+	[[Category: Gold M]]
-	[[Category: Maxwell~~, K L~~]]	+	[[Category: Maxwell KL]]
-	[[Category: Yee~~, A A]]~~	+	[[Category: Yee AA]]
-	~~[[Category: Twisted beta-sandwich]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 08:06, 23 February 2022

OCA — Wed, 23 Feb 2022 08:06:19 GMT

←Older revision		Revision as of 08:06, 23 February 2022
Line 3:		Line 3:
	<StructureSection load='1k0h' size='340' side='right'caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1k0h' size='340' side='right'caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1K0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [~~http~~://pdbe.org/1k0h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [https://pdbe.org/1k0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [https://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>	+	[[https://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:38, 25 November 2020

OCA — Wed, 25 Nov 2020 07:38:41 GMT

←Older revision		Revision as of 07:38, 25 November 2020
Line 1:		Line 1:

	==Solution structure of bacteriophage lambda gpFII==		==Solution structure of bacteriophage lambda gpFII==
-	<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='1k0h' size='340' side='right'caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1K0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://pdbe.org/1k0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://pdbe.org/1k0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacteriophage lambda]]		[[Category: Bacteriophage lambda]]
		+	[[Category: Large Structures]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
	[[Category: Davidson, A R]]		[[Category: Davidson, A R]]

OCA at 09:12, 17 January 2018

OCA — Wed, 17 Jan 2018 09:12:31 GMT

←Older revision		Revision as of 09:12, 17 January 2018
Line 1:		Line 1:
		+
	==Solution structure of bacteriophage lambda gpFII==		==Solution structure of bacteriophage lambda gpFII==
	<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>		<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://pdbe.org/1k0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://pdbe.org/1k0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k0h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0h_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0h_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:30, 8 February 2016

OCA — Mon, 08 Feb 2016 15:30:38 GMT

←Older revision		Revision as of 15:30, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k0h ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:58, 10 September 2015

OCA — Thu, 10 Sep 2015 12:58:26 GMT

←Older revision		Revision as of 12:58, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Enterobacteria_phage_lambda Enterobacteria phage~~ lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 ~~Enterobacteria phage~~ lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://pdbe.org/1k0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~VCF2_LAMBD VCF2_LAMBD~~]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:~~5022189~~</ref> <ref>PMID:~~4413519~~</ref> <ref>PMID:~~4616093~~</ref>	+	[[http://www.uniprot.org/uniprot/FII_LAMBD FII_LAMBD]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:4413519</ref> <ref>PMID:4616093</ref> <ref>PMID:5022189</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1k0h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enterobacteria phage~~ lambda]]	+	[[Category: Bacteriophage lambda]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
	[[Category: Davidson, A R]]		[[Category: Davidson, A R]]

OCA at 00:21, 25 December 2014

OCA — Thu, 25 Dec 2014 00:21:07 GMT

←Older revision		Revision as of 00:21, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>		<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VCF2_LAMBD VCF2_LAMBD]] Plays a role in virion assembly by joining the head and the tail at the last step of morphogenesis. Six FII proteins bind the DNA-filled capsid after W binding, in turn binding the pre-assembled tail by interacting with U protein.<ref>PMID:5022189</ref> <ref>PMID:4413519</ref> <ref>PMID:4616093</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
-	[[Category: Arrowsmith, C H.]]	+	[[Category: Arrowsmith, C H]]
-	[[Category: Davidson, A R.]]	+	[[Category: Davidson, A R]]
-	[[Category: Gold, M.]]	+	[[Category: Gold, M]]
-	[[Category: Maxwell, K L.]]	+	[[Category: Maxwell, K L]]
-	[[Category: Yee, A A.]]	+	[[Category: Yee, A A]]
	[[Category: Twisted beta-sandwich]]		[[Category: Twisted beta-sandwich]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 11:25, 28 September 2014

OCA — Sun, 28 Sep 2014 11:25:13 GMT

←Older revision		Revision as of 11:25, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1k0h.~~png\|left~~\|200px]]	+	==Solution structure of bacteriophage lambda gpFII==
		+	<StructureSection load='1k0h' size='340' side='right' caption='[[1k0h]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1k0h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K0H FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k0h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k0h RCSB], [http://www.ebi.ac.uk/pdbsum/1k0h PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/1k0h_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacteriophage lambda FII protein (gpFII) is a 117 residue structural protein found in the phage particle that is required for the joining of phage heads and tails at the last step of morphogenesis. We have performed biophysical experiments to show that gpFII is stable, monomeric, and reversibly folded. We have also determined the atomic resolution structure of gpFII using NMR spectroscopy. gpFII is shown to possess a novel fold consisting of seven beta-strands and a short alpha-helix. It also displays two large unstructured regions at the N terminus (residues 1-24) and in a large loop near the middle of the protein (residues 46-62). We speculate that these unstructured regions become structured when gpFII assembles into the phage particle, and that these conformational changes play an important role in regulating the assembly pathway. Alignment of the gpFII sequence with those of homologues from other lambdoid phages has allowed us to putatively identify distinct surfaces on the gpFII structure that mediate binding to the phage head and tail.

-	~~{{STRUCTURE_1k0h\| PDB=1k0h \| SCENE= }}~~	+	The solution structure of the bacteriophage lambda head-tail joining protein, gpFII.,Maxwell KL, Yee AA, Arrowsmith CH, Gold M, Davidson AR J Mol Biol. 2002 May 17;318(5):1395-404. PMID:12083526<ref>PMID:12083526</ref>

-	~~===Solution structure~~ of ~~bacteriophage lambda gpFII===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12083526}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1k0h]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1K0H OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012083526<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Enterobacteria phage lambda]]		[[Category: Enterobacteria phage lambda]]
	[[Category: Arrowsmith, C H.]]		[[Category: Arrowsmith, C H.]]

OCA: Protected "1k0h" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 11:26:27 GMT

Protected "1k0h" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 5 December 2012