1k25 - Revision history

OCA at 07:43, 7 February 2024

OCA — Wed, 07 Feb 2024 07:43:48 GMT

←Older revision		Revision as of 07:43, 7 February 2024
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	<StructureSection load='1k25' size='340' side='right'caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1k25' size='340' side='right'caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K25 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1qmf\|1qmf]]~~, [[~~1qme~~\|~~1qme~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [https://pdbe.org/1k25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [https://www.ebi.ac.uk/pdbsum/1k25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k25 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [https://pdbe.org/1k25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [https://www.ebi.ac.uk/pdbsum/1k25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k25 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O34006_STREE O34006_STREE]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k25 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k25 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Penicillin-binding proteins (PBPs) are the main targets for beta-lactam antibiotics, such as penicillins and cephalosporins, in a wide range of bacterial species. In some Gram-positive strains, the surge of resistance to treatment with beta-lactams is primarily the result of the proliferation of mosaic PBP-encoding genes, which encode novel proteins by recombination. PBP2x is a primary resistance determinant in Streptococcus pneumoniae, and its modification is an essential step in the development of high level beta-lactam resistance. To understand such a resistance mechanism at an atomic level, we have solved the x-ray crystal structure of PBP2x from a highly penicillin-resistant clinical isolate of S. pneumoniae, Sp328, which harbors 83 mutations in the soluble region. In the proximity of the Sp328 PBP2x* active site, the Thr(338) --> Ala mutation weakens the local hydrogen bonding network, thus abrogating the stabilization of a crucial buried water molecule. In addition, the Ser(389) --> Leu and Asn(514) --> His mutations produce a destabilizing effect that generates an "open" active site. It has been suggested that peptidoglycan substrates for beta-lactam-resistant PBPs contain a large amount of abnormal, branched peptides, whereas sensitive strains tend to catalyze cross-linking of linear forms. Thus, in vivo, an "open" active site could facilitate the recognition of distinct, branched physiological substrates.
-
-	Crystal structure of PBP2x from a highly penicillin-resistant Streptococcus pneumoniae clinical isolate: a mosaic framework containing 83 mutations.,Dessen A, Mouz N, Gordon E, Hopkins J, Dideberg O J Biol Chem. 2001 Nov 30;276(48):45106-12. Epub 2001 Sep 11. PMID:11553637<ref>PMID:11553637</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]		*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dessen, A]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: Dideberg, O]]	+	[[Category: Dessen A]]
-	[[Category: Hopkins, J]]	+	[[Category: Dideberg O]]
-	[[Category: Mouz, N]]	+	[[Category: Hopkins J]]
-	~~[[Category: Antibiotic resistance]]~~	+	[[Category: Mouz N]]
-	~~[[Category: Clinical mutant]]~~	+
-	~~[[Category: Low-affinity penicillin-binding]]~~	+
-	~~[[Category: Membrane protein~~]]	+

OCA at 06:48, 11 August 2021

OCA — Wed, 11 Aug 2021 06:48:26 GMT

←Older revision		Revision as of 06:48, 11 August 2021
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	<StructureSection load='1k25' size='340' side='right'caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1k25' size='340' side='right'caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1K25 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qmf\|1qmf]], [[1qme\|1qme]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [~~http~~://pdbe.org/1k25 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1k25 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1k25 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [https://pdbe.org/1k25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [https://www.ebi.ac.uk/pdbsum/1k25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k25 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[Penicillin-binding protein\|Penicillin-binding protein]]	+	*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:10, 6 November 2019

OCA — Wed, 06 Nov 2019 09:10:06 GMT

←Older revision		Revision as of 09:10, 6 November 2019
Line 1:		Line 1:

	==PBP2x from a Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate==		==PBP2x from a Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate==
-	<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='1k25' size='340' side='right'caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Penicillin-binding protein\|Penicillin-binding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Dessen, A]]		[[Category: Dessen, A]]
	[[Category: Dideberg, O]]		[[Category: Dideberg, O]]

OCA at 21:19, 24 January 2018

OCA — Wed, 24 Jan 2018 21:19:57 GMT

←Older revision		Revision as of 21:19, 24 January 2018
Line 1:		Line 1:
		+
	==PBP2x from a Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate==		==PBP2x from a Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate==
	<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://pdbe.org/1k25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://pdbe.org/1k25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k25 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k25_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k25_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Penicillin-binding protein\|Penicillin-binding protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 23:07, 8 February 2016

OCA — Mon, 08 Feb 2016 23:07:27 GMT

←Older revision		Revision as of 23:07, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k25 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:14, 10 September 2015

OCA — Thu, 10 Sep 2015 16:14:22 GMT

←Older revision		Revision as of 16:14, 10 September 2015
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	<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae Streptococcus~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://pdbe.org/1k25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1k25" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptococcus pneumoniae]]
	[[Category: Dessen, A]]		[[Category: Dessen, A]]
	[[Category: Dideberg, O]]		[[Category: Dideberg, O]]

OCA at 15:30, 5 January 2015

OCA — Mon, 05 Jan 2015 15:30:02 GMT

←Older revision		Revision as of 15:30, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
-	[[Category: Dessen, A.]]	+	[[Category: Dessen, A]]
-	[[Category: Dideberg, O.]]	+	[[Category: Dideberg, O]]
-	[[Category: Hopkins, J.]]	+	[[Category: Hopkins, J]]
-	[[Category: Mouz, N.]]	+	[[Category: Mouz, N]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Clinical mutant]]		[[Category: Clinical mutant]]
	[[Category: Low-affinity penicillin-binding]]		[[Category: Low-affinity penicillin-binding]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]

OCA at 08:28, 28 September 2014

OCA — Sun, 28 Sep 2014 08:28:07 GMT

←Older revision		Revision as of 08:28, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1k25.~~png~~\|~~left~~\|200px]]	+	==PBP2x from a Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate==
		+	<StructureSection load='1k25' size='340' side='right' caption='[[1k25]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K25 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qmf\|1qmf]], [[1qme\|1qme]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k25 RCSB], [http://www.ebi.ac.uk/pdbsum/1k25 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k2/1k25_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Penicillin-binding proteins (PBPs) are the main targets for beta-lactam antibiotics, such as penicillins and cephalosporins, in a wide range of bacterial species. In some Gram-positive strains, the surge of resistance to treatment with beta-lactams is primarily the result of the proliferation of mosaic PBP-encoding genes, which encode novel proteins by recombination. PBP2x is a primary resistance determinant in Streptococcus pneumoniae, and its modification is an essential step in the development of high level beta-lactam resistance. To understand such a resistance mechanism at an atomic level, we have solved the x-ray crystal structure of PBP2x from a highly penicillin-resistant clinical isolate of S. pneumoniae, Sp328, which harbors 83 mutations in the soluble region. In the proximity of the Sp328 PBP2x* active site, the Thr(338) --> Ala mutation weakens the local hydrogen bonding network, thus abrogating the stabilization of a crucial buried water molecule. In addition, the Ser(389) --> Leu and Asn(514) --> His mutations produce a destabilizing effect that generates an "open" active site. It has been suggested that peptidoglycan substrates for beta-lactam-resistant PBPs contain a large amount of abnormal, branched peptides, whereas sensitive strains tend to catalyze cross-linking of linear forms. Thus, in vivo, an "open" active site could facilitate the recognition of distinct, branched physiological substrates.

-	~~{{STRUCTURE_1k25\| PDB=1k25 \| SCENE= }}~~	+	Crystal structure of PBP2x from a highly penicillin-resistant Streptococcus pneumoniae clinical isolate: a mosaic framework containing 83 mutations.,Dessen A, Mouz N, Gordon E, Hopkins J, Dideberg O J Biol Chem. 2001 Nov 30;276(48):45106-12. Epub 2001 Sep 11. PMID:11553637<ref>PMID:11553637</ref>

-	~~===PBP2x from~~ a ~~Highly Penicillin-resistant Streptococcus pneumoniae Clinical Isolate===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11553637}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1k25]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA].~~	+

	==See Also==		==See Also==
	*[[Penicillin-binding protein\|Penicillin-binding protein]]		*[[Penicillin-binding protein\|Penicillin-binding protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011553637<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
	[[Category: Dessen, A.]]		[[Category: Dessen, A.]]

OCA at 11:47, 20 October 2012

OCA — Sat, 20 Oct 2012 11:47:28 GMT

←Older revision		Revision as of 11:47, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1k25]] is a 4 chain structure ~~of [[Penicillin-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA].	+	[[1k25]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K25 OCA].

	==See Also==		==See Also==

OCA: Protected "1k25" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 06:37:03 GMT

Protected "1k25" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:37, 26 July 2012