1k9n - Revision history

OCA at 06:50, 11 August 2021

OCA — Wed, 11 Aug 2021 06:50:57 GMT

←Older revision		Revision as of 06:50, 11 August 2021
Line 4:		Line 4:
	<StructureSection load='1k9n' size='340' side='right'caption='[[1k9n]]' scene=''>		<StructureSection load='1k9n' size='340' side='right'caption='[[1k9n]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1K9N FirstGlance]. <br>	+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K9N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1k9n FirstGlance], [~~http~~://www.ebi.ac.uk/pdbsum/1k9n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9n ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k9n FirstGlance], [https://www.ebi.ac.uk/pdbsum/1k9n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:30, 6 November 2019

OCA — Wed, 06 Nov 2019 09:30:45 GMT

←Older revision		Revision as of 09:30, 6 November 2019
Line 2:		Line 2:

	==MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A==		==MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A==
-	<StructureSection load='1k9n' size='340' side='right' caption='[[1k9n]]' scene=''>	+	<StructureSection load='1k9n' size='340' side='right'caption='[[1k9n]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K9N FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K9N FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Theoretical Model]]		[[Category: Theoretical Model]]
		+	[[Category: Large Structures]]
	[[Category: Karim, C B]]		[[Category: Karim, C B]]
	[[Category: Karim, J]]		[[Category: Karim, J]]
	[[Category: Paterlini, M G]]		[[Category: Paterlini, M G]]
	[[Category: Thomas, D D]]		[[Category: Thomas, D D]]

OCA at 21:37, 24 January 2018

OCA — Wed, 24 Jan 2018 21:37:17 GMT

←Older revision		Revision as of 21:37, 24 January 2018
Line 5:		Line 5:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K9N FirstGlance]. <br>		<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K9N FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k9n FirstGlance], [http://www.ebi.ac.uk/pdbsum/1k9n PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k9n FirstGlance], [http://www.ebi.ac.uk/pdbsum/1k9n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:09, 11 February 2016

OCA — Thu, 11 Feb 2016 09:09:48 GMT

←Older revision		Revision as of 09:09, 11 February 2016
Line 1:		Line 1:
		+	{{Theoretical_model}}
		+
	==MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A==		==MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A==
	<StructureSection load='1k9n' size='340' side='right' caption='[[1k9n]]' scene=''>		<StructureSection load='1k9n' size='340' side='right' caption='[[1k9n]]' scene=''>
Line 18:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Theoretical Model]]
	[[Category: Karim, C B]]		[[Category: Karim, C B]]
	[[Category: Karim, J]]		[[Category: Karim, J]]
	[[Category: Paterlini, M G]]		[[Category: Paterlini, M G]]
	[[Category: Thomas, D D]]		[[Category: Thomas, D D]]

OCA at 07:12, 10 September 2015

OCA — Thu, 10 Sep 2015 07:12:18 GMT

←Older revision		Revision as of 07:12, 10 September 2015
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1k9n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:28, 18 December 2014

OCA — Thu, 18 Dec 2014 12:28:14 GMT

←Older revision		Revision as of 12:28, 18 December 2014
Line 1:		Line 1:
-	~~{{Theoretical_model}}~~	+	==MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A==
		+	<StructureSection load='1k9n' size='340' side='right' caption='[[1k9n]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K9N FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k9n FirstGlance], [http://www.ebi.ac.uk/pdbsum/1k9n PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To test models for the pentameric structure of phospholamban (PLB) and study its structure and molecular dynamics in SDS solution, we characterized recombinant PLB and several of its mutants by (a) reactivity of cysteine residues toward DTNB [5, 5'-dithiobis(2-nitrobenzoic acid)] and a thiol-reactive spin label, (b) oligomeric state on SDS-PAGE, and (c) EPR of the spin-labeled proteins. WT-PLB has three cysteine residues (36, 41, and 46), all located in the hydrophobic C-terminal transmembrane region. In SDS at pH 7.5, exhaustive reaction with either sulfhydryl reagent resulted in essentially 2 mol of cysteine reacted/mol of WT-PLB, with only slight destabilization of the native pentameric structure. When WT-PLB was denatured in guanidine at pH 8.1, all three cysteines reacted, disrupting the pentamer, which was restored upon cleavage of the disulfide bonds with DTT. In the tetrameric mutant C41L-PLB, the two remaining cysteine residues reacted, reversibly destabilizing the tetramer. In the monomeric mutant L37A-PLB, all three cysteines reacted. The pentameric double cysteine replacement mutant C36,46A-PLB showed negligible reactivity. We conclude that Cys-41 is the unreactive cysteine in PLB and is located at a crucial site for the maintenance of the pentameric structure. EPR spectra in SDS of spin-labeled WT-PLB and mutants correlate with the oligomeric state on SDS-PAGE; oligomeric proteins show decreased spin-label mobility compared with monomers. Molecular dynamics calculations were used to construct an atomic model for the transmembrane region of the PLB pentamer, constrained by previous mutagenesis results and the results of the present study. We conclude that (a) the mobilities of spin-labels attached to PLB and its mutants are sensitive to oligomeric state and (b) the pattern of cysteine reactivity, spin-label mobility, and oligomeric state supports a structural model for the PLB pentamer in which interactions between each pair of subunits are stabilized by a leucine-isoleucine zipper.

-	~~{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}~~	+	Cysteine reactivity and oligomeric structures of phospholamban and its mutants.,Karim CB, Stamm JD, Karim J, Jones LR, Thomas DD Biochemistry. 1998 Sep 1;37(35):12074-81. PMID:9724519<ref>PMID:9724519</ref>
-	~~===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN~~, ~~A REGULATOR OF THE CALCIUM ATPASE SERCA2A===~~	+
-	~~{{ABSTRACT_PUBMED_9724519}}~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:009724519</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Karim, C B]]		[[Category: Karim, C B]]
	[[Category: Karim, J]]		[[Category: Karim, J]]
	[[Category: Paterlini, M G]]		[[Category: Paterlini, M G]]
	[[Category: Thomas, D D]]		[[Category: Thomas, D D]]

OCA at 03:49, 25 March 2013

OCA — Mon, 25 Mar 2013 03:49:18 GMT

←Older revision		Revision as of 03:49, 25 March 2013
Line 1:		Line 1:
	{{Theoretical_model}}		{{Theoretical_model}}
-
-	[[Image:1k9n.png\|left\|200px]]

	{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}		{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}
-
	===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A===		===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A===
-
	{{ABSTRACT_PUBMED_9724519}}		{{ABSTRACT_PUBMED_9724519}}

	==Reference==		==Reference==
-	<ref group="xtra">PMID:009724519</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009724519</ref><references group="xtra"/><references/>
	[[Category: Karim, C B]]		[[Category: Karim, C B]]
	[[Category: Karim, J]]		[[Category: Karim, J]]
	[[Category: Paterlini, M G]]		[[Category: Paterlini, M G]]
	[[Category: Thomas, D D]]		[[Category: Thomas, D D]]

OCA: Protected "1k9n" [edit=sysop:move=sysop]

OCA — Sun, 21 Oct 2012 09:08:16 GMT

Protected "1k9n" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:08, 21 October 2012

OCA at 09:08, 21 October 2012

OCA — Sun, 21 Oct 2012 09:08:12 GMT

←Older revision		Revision as of 09:08, 21 October 2012
Line 1:		Line 1:
	{{Theoretical_model}}		{{Theoretical_model}}
-	~~{{Seed}}~~	+
	[[Image:1k9n.png\|left\|200px]]		[[Image:1k9n.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1k9n", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}		{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}

	===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A===		===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9724519}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9724519 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9724519}}		{{ABSTRACT_PUBMED_9724519}}
-
-	==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9N OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9724519~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009724519</ref><references group="xtra"/>
	[[Category: Karim, C B]]		[[Category: Karim, C B]]
	[[Category: Karim, J]]		[[Category: Karim, J]]
	[[Category: Paterlini, M G]]		[[Category: Paterlini, M G]]
	[[Category: Thomas, D D]]		[[Category: Thomas, D D]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:44:38 2010''

OCA at 04:44, 8 April 2010

OCA — Thu, 08 Apr 2010 04:44:39 GMT

←Older revision		Revision as of 04:44, 8 April 2010
Line 1:		Line 1:
-	~~'''Theoretical Model'''~~	+	{{Theoretical_model}}
		+	{{Seed}}
		+	[[Image:1k9n.png\|left\|200px]]

-	The ~~entry 1K9N~~ is ~~a Theoretical Model titled 'MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN~~, ~~A REGULATOR OF THE CALCIUM ATPASE SERCA2A'~~.	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_1k9n", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_1k9n\| PDB=1k9n \| SCENE= }}

-	~~[[Category:Theoretical Model]]~~	+	===MODEL OF THE PENTAMERIC TRANSMEMBRANE DOMAIN OF PHOSPHOLAMBAN, A REGULATOR OF THE CALCIUM ATPASE SERCA2A===


-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 30 09~~:50:~~34 2008~~''	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_9724519}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9724519 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9724519}}
		+
		+	==About this Structure==
		+	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9N OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:9724519</ref><references group="xtra"/>
		+	[[Category: Karim, C B]]
		+	[[Category: Karim, J]]
		+	[[Category: Paterlini, M G]]
		+	[[Category: Thomas, D D]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 8 07:44:38 2010''