1kan - Revision history

OCA at 07:45, 7 February 2024

OCA — Wed, 07 Feb 2024 07:45:29 GMT

←Older revision		Revision as of 07:45, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [https://pdbe.org/1kan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [https://www.ebi.ac.uk/pdbsum/1kan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [https://pdbe.org/1kan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [https://www.ebi.ac.uk/pdbsum/1kan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.		[https://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Kanamycin nucleotidyltransferase, as originally isolated from Staphylococcus aureus, inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside. The molecular structure of the enzyme described here was determined by X-ray crystallographic analysis to a resolution of 3.0 A. Crystals employed in the investigation belonged to the space group P4(3)2(1)2 with unit cell dimensions of a = b = 78.9 A and c = 219.2 A. An electron density map phased with seven heavy-atom derivatives revealed that the molecules packed in the crystalline lattice as dimers exhibiting local 2-fold rotation axes. Subsequent symmetry averaging and solvent flattening improved the quality of the electron density such that it was possible to completely trace the 253 amino acid polypeptide chain. Each monomer is divided into two distinct structural domains: the N-terminal motif composed of residues Met 1-Glu 127 and the C-terminal half delineated by residues Ala 128-Phe 253. The N-terminal region is characterized by a five-stranded mixed beta-pleated sheet whereas the C-terminal domain contains five alpha-helices, four of which form an up-and-down alpha-helical bundle very similar to that observed in cytochrome c'. The two subunits wrap about one another to form an ellipsoid with a pronounced cleft that could easily accommodate the various aminoglycosides known to bind to the enzyme.
-
-	Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution.,Sakon J, Liao HH, Kanikula AM, Benning MM, Rayment I, Holden HM Biochemistry. 1993 Nov 16;32(45):11977-84. PMID:8218273<ref>PMID:8218273</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1kan" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:07, 25 January 2023

OCA — Wed, 25 Jan 2023 07:07:32 GMT

←Older revision		Revision as of 07:07, 25 January 2023
Line 3:		Line 3:
	<StructureSection load='1kan' size='340' side='right'caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1kan' size='340' side='right'caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [https://pdbe.org/1kan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [https://www.ebi.ac.uk/pdbsum/1kan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [https://pdbe.org/1kan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [https://www.ebi.ac.uk/pdbsum/1kan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU]] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.	+	[https://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Holden, H M~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Rayment, I~~]]	+	[[Category: Holden HM]]
-	[[Category: ~~Sakon, J~~]]	+	[[Category: Rayment I]]
-	[[Category: ~~Nucleotidyltransferase~~]]	+	[[Category: Sakon J]]

OCA at 08:46, 21 April 2021

OCA — Wed, 21 Apr 2021 08:46:40 GMT

←Older revision		Revision as of 08:46, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1kan' size='340' side='right'caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1kan' size='340' side='right'caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [~~http~~://pdbe.org/1kan PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1kan PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [https://pdbe.org/1kan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [https://www.ebi.ac.uk/pdbsum/1kan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU]] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.	+	[[https://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU]] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:56, 17 July 2019

OCA — Wed, 17 Jul 2019 12:56:35 GMT

←Older revision		Revision as of 12:56, 17 July 2019
Line 1:		Line 1:

	==MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION==		==MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION==
-	<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='1kan' size='340' side='right'caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]
	[[Category: Rayment, I]]		[[Category: Rayment, I]]
	[[Category: Sakon, J]]		[[Category: Sakon, J]]
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]

OCA at 07:13, 11 October 2017

OCA — Wed, 11 Oct 2017 07:13:33 GMT

←Older revision		Revision as of 07:13, 11 October 2017
Line 1:		Line 1:
		+
	==MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION==		==MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION==
	<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://pdbe.org/1kan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://pdbe.org/1kan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kan ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:11, 11 September 2015

OCA — Fri, 11 Sep 2015 08:11:41 GMT

←Older revision		Revision as of 08:11, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://pdbe.org/1kan PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1kan" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]
	[[Category: Rayment, I]]		[[Category: Rayment, I]]
	[[Category: Sakon, J]]		[[Category: Sakon, J]]
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]

OCA at 13:41, 25 December 2014

OCA — Thu, 25 Dec 2014 13:41:51 GMT

←Older revision		Revision as of 13:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KANU_STAAU KANU_STAAU]] Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Holden, H M.]]	+	[[Category: Holden, H M]]
-	[[Category: Rayment, I.]]	+	[[Category: Rayment, I]]
-	[[Category: Sakon, J.]]	+	[[Category: Sakon, J]]
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]

OCA at 05:28, 8 June 2014

OCA — Sun, 08 Jun 2014 05:28:28 GMT

←Older revision		Revision as of 05:28, 8 June 2014
Line 1:		Line 1:
-	[[~~Image~~:1kan.~~png\|left\|200px~~]]	+	==MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION==
		+	<StructureSection load='1kan' size='340' side='right' caption='[[1kan]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KAN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kan OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kan RCSB], [http://www.ebi.ac.uk/pdbsum/1kan PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kanamycin nucleotidyltransferase, as originally isolated from Staphylococcus aureus, inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside. The molecular structure of the enzyme described here was determined by X-ray crystallographic analysis to a resolution of 3.0 A. Crystals employed in the investigation belonged to the space group P4(3)2(1)2 with unit cell dimensions of a = b = 78.9 A and c = 219.2 A. An electron density map phased with seven heavy-atom derivatives revealed that the molecules packed in the crystalline lattice as dimers exhibiting local 2-fold rotation axes. Subsequent symmetry averaging and solvent flattening improved the quality of the electron density such that it was possible to completely trace the 253 amino acid polypeptide chain. Each monomer is divided into two distinct structural domains: the N-terminal motif composed of residues Met 1-Glu 127 and the C-terminal half delineated by residues Ala 128-Phe 253. The N-terminal region is characterized by a five-stranded mixed beta-pleated sheet whereas the C-terminal domain contains five alpha-helices, four of which form an up-and-down alpha-helical bundle very similar to that observed in cytochrome c'. The two subunits wrap about one another to form an ellipsoid with a pronounced cleft that could easily accommodate the various aminoglycosides known to bind to the enzyme.

-	~~{{STRUCTURE_1kan\| PDB=1kan \| SCENE= }}~~	+	Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution.,Sakon J, Liao HH, Kanikula AM, Benning MM, Rayment I, Holden HM Biochemistry. 1993 Nov 16;32(45):11977-84. PMID:8218273<ref>PMID:8218273</ref>

-	~~===MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8218273}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1kan]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008218273<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Holden, H M.]]		[[Category: Holden, H M.]]

OCA: Protected "1kan" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 17:13:23 GMT

Protected "1kan" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:13, 14 August 2012

OCA at 17:13, 14 August 2012

OCA — Tue, 14 Aug 2012 17:13:20 GMT

←Older revision		Revision as of 17:13, 14 August 2012
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-	{{Seed}}
	[[Image:1kan.png\|left\|200px]]		[[Image:1kan.png\|left\|200px]]

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	{{STRUCTURE_1kan\| PDB=1kan \| SCENE= }}		{{STRUCTURE_1kan\| PDB=1kan \| SCENE= }}

	===MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION===		===MOLECULAR STRUCTURE OF KANAMYCIN NUCLEOTIDYLTRANSFERASE DETERMINED TO 3.0-ANGSTROMS RESOLUTION===

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-	<!--
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	{{ABSTRACT_PUBMED_8218273}}		{{ABSTRACT_PUBMED_8218273}}

	==About this Structure==		==About this Structure==
-	~~1KAN is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA].	+	[[1kan]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KAN OCA].

	==Reference==		==Reference==
-	~~Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution., Sakon J, Liao HH, Kanikula AM, Benning MM, Rayment I, Holden HM, Biochemistry. 1993 Nov 16;32(45):11977-84.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/8218273 8218273]~~	+	<ref group="xtra">PMID:008218273</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Holden, H M.]]		[[Category: Holden, H M.]]
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	[[Category: Sakon, J.]]		[[Category: Sakon, J.]]
	[[Category: Nucleotidyltransferase]]		[[Category: Nucleotidyltransferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:02:40 2008''