1kjx - Revision history

OCA at 07:25, 14 February 2024

OCA — Wed, 14 Feb 2024 07:25:35 GMT

←Older revision		Revision as of 07:25, 14 February 2024
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	<StructureSection load='1kjx' size='340' side='right'caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1kjx' size='340' side='right'caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1cib\|1cib]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [https://pdbe.org/1kjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [https://www.ebi.ac.uk/pdbsum/1kjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [https://pdbe.org/1kjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [https://www.ebi.ac.uk/pdbsum/1kjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PURA_ECOLI PURA_ECOLI]] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).[HAMAP-Rule:MF_00011]	+	[https://www.uniprot.org/uniprot/PURA_ECOLI PURA_ECOLI] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).[HAMAP-Rule:MF_00011]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kjx ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kjx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A complete set of substrate/substrate analogs of adenylosuccinate synthetase from Escherichia coli induces dimer formation and a transition from a disordered to an ordered active site. The most striking of the ligand-induced effects is the movement of loop 40-53 by up to 9 A. Crystal structures of the partially ligated synthetase, which either combine IMP and hadacidin or IMP, hadacidin, and Mg(2+)-pyrophosphate, have ordered active sites, comparable with the fully ligated enzyme. More significantly, a crystal structure of the synthetase with IMP alone exhibits a largely ordered active site, which includes the 9 A movement of loop 40-53 but does not include conformational adjustments to backbone carbonyl 40 (Mg(2+) interaction element) and loop 298-304 (L-aspartate binding element). Interactions involving the 5'-phosphoryl group of IMP evidently trigger the formation of salt links some 30 A away. The above provides a structural basis for ligand binding synergism, effects on k(cat) due to mutations far from the site of catalysis, and the complete loss of substrate efficacy due to minor alterations of the 5'-phosphoryl group of IMP.
-
-	IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli.,Hou Z, Wang W, Fromm HJ, Honzatko RB J Biol Chem. 2002 Feb 22;277(8):5970-6. Epub 2001 Dec 12. PMID:11741996<ref>PMID:11741996</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Adenylosuccinate synthetase 3D structures\|Adenylosuccinate synthetase 3D structures]]		*[[Adenylosuccinate synthetase 3D structures\|Adenylosuccinate synthetase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Adenylosuccinate synthase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fromm~~, H J~~]]	+	[[Category: Fromm HJ]]
-	[[Category: Honzatko~~, R B~~]]	+	[[Category: Honzatko RB]]
-	[[Category: Hou, Z]]	+	[[Category: Hou Z]]
-	[[Category: Wang, W]]	+	[[Category: Wang W]]
-	~~[[Category: Gtp-hydrolysing enzyme]]~~	+
-	~~[[Category: Imp]]~~	+
-	~~[[Category: Induced fit]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Purine nucleotide biosynthesis~~]]	+

OCA at 08:48, 21 April 2021

OCA — Wed, 21 Apr 2021 08:48:24 GMT

←Older revision		Revision as of 08:48, 21 April 2021
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	<StructureSection load='1kjx' size='340' side='right'caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1kjx' size='340' side='right'caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1cib\|1cib]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [~~http~~://pdbe.org/1kjx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1kjx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [https://pdbe.org/1kjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [https://www.ebi.ac.uk/pdbsum/1kjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PURA_ECOLI PURA_ECOLI]] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).[HAMAP-Rule:MF_00011]	+	[[https://www.uniprot.org/uniprot/PURA_ECOLI PURA_ECOLI]] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).[HAMAP-Rule:MF_00011]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:55, 17 July 2019

OCA — Wed, 17 Jul 2019 12:55:26 GMT

←Older revision		Revision as of 12:55, 17 July 2019
Line 1:		Line 1:

	==IMP Complex of E. Coli Adenylosuccinate Synthetase==		==IMP Complex of E. Coli Adenylosuccinate Synthetase==
-	<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='1kjx' size='340' side='right'caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/1kjx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/1kjx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Adenylosuccinate synthetase 3D structures\|Adenylosuccinate synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 39:
	[[Category: Adenylosuccinate synthase]]		[[Category: Adenylosuccinate synthase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Fromm, H J]]		[[Category: Fromm, H J]]
	[[Category: Honzatko, R B]]		[[Category: Honzatko, R B]]

OCA at 12:18, 12 October 2017

OCA — Thu, 12 Oct 2017 12:18:33 GMT

←Older revision		Revision as of 12:18, 12 October 2017
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://pdbe.org/1kjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://pdbe.org/1kjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kjx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Adenylosuccinate Synthetase\|Adenylosuccinate Synthetase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:26, 1 June 2016

OCA — Wed, 01 Jun 2016 07:26:13 GMT

←Older revision		Revision as of 07:26, 1 June 2016
Line 1:		Line 1:
		+
	==IMP Complex of E. Coli Adenylosuccinate Synthetase==		==IMP Complex of E. Coli Adenylosuccinate Synthetase==
	<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>

OCA at 21:58, 7 February 2016

OCA — Sun, 07 Feb 2016 21:58:22 GMT

←Older revision		Revision as of 21:58, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kjx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:18, 10 September 2015

OCA — Thu, 10 Sep 2015 05:18:48 GMT

←Older revision		Revision as of 05:18, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli_k-12 Escherichia coli k-12~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://pdbe.org/1kjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1kjx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Adenylosuccinate synthase]]		[[Category: Adenylosuccinate synthase]]
-	[[Category: ~~Escherichia coli k-12~~]]	+	[[Category: Ecoli]]
	[[Category: Fromm, H J]]		[[Category: Fromm, H J]]
	[[Category: Honzatko, R B]]		[[Category: Honzatko, R B]]

OCA at 18:30, 24 December 2014

OCA — Wed, 24 Dec 2014 18:30:08 GMT

←Older revision		Revision as of 18:30, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PURA_ECOLI PURA_ECOLI]] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).[HAMAP-Rule:MF_00011]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Adenylosuccinate synthase]]		[[Category: Adenylosuccinate synthase]]
	[[Category: Escherichia coli k-12]]		[[Category: Escherichia coli k-12]]
-	[[Category: Fromm, H J.]]	+	[[Category: Fromm, H J]]
-	[[Category: Honzatko, R B.]]	+	[[Category: Honzatko, R B]]
-	[[Category: Hou, Z.]]	+	[[Category: Hou, Z]]
-	[[Category: Wang, W.]]	+	[[Category: Wang, W]]
	[[Category: Gtp-hydrolysing enzyme]]		[[Category: Gtp-hydrolysing enzyme]]
	[[Category: Imp]]		[[Category: Imp]]

OCA at 09:53, 28 September 2014

OCA — Sun, 28 Sep 2014 09:53:22 GMT

←Older revision		Revision as of 09:53, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1kjx.~~png~~\|~~left~~\|200px]]	+	==IMP Complex of E. Coli Adenylosuccinate Synthetase==
		+	<StructureSection load='1kjx' size='340' side='right' caption='[[1kjx]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KJX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1cib\|1cib]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylosuccinate_synthase Adenylosuccinate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.4 6.3.4.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kjx RCSB], [http://www.ebi.ac.uk/pdbsum/1kjx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kj/1kjx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A complete set of substrate/substrate analogs of adenylosuccinate synthetase from Escherichia coli induces dimer formation and a transition from a disordered to an ordered active site. The most striking of the ligand-induced effects is the movement of loop 40-53 by up to 9 A. Crystal structures of the partially ligated synthetase, which either combine IMP and hadacidin or IMP, hadacidin, and Mg(2+)-pyrophosphate, have ordered active sites, comparable with the fully ligated enzyme. More significantly, a crystal structure of the synthetase with IMP alone exhibits a largely ordered active site, which includes the 9 A movement of loop 40-53 but does not include conformational adjustments to backbone carbonyl 40 (Mg(2+) interaction element) and loop 298-304 (L-aspartate binding element). Interactions involving the 5'-phosphoryl group of IMP evidently trigger the formation of salt links some 30 A away. The above provides a structural basis for ligand binding synergism, effects on k(cat) due to mutations far from the site of catalysis, and the complete loss of substrate efficacy due to minor alterations of the 5'-phosphoryl group of IMP.

-	~~{{STRUCTURE_1kjx\| PDB=1kjx \| SCENE= }}~~	+	IMP Alone Organizes the Active Site of Adenylosuccinate Synthetase from Escherichia coli.,Hou Z, Wang W, Fromm HJ, Honzatko RB J Biol Chem. 2002 Feb 22;277(8):5970-6. Epub 2001 Dec 12. PMID:11741996<ref>PMID:11741996</ref>

-	~~===IMP Complex~~ of E. ~~Coli Adenylosuccinate Synthetase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11741996}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1kjx]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA].~~	+

	==See Also==		==See Also==
	*[[Adenylosuccinate Synthetase\|Adenylosuccinate Synthetase]]		*[[Adenylosuccinate Synthetase\|Adenylosuccinate Synthetase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011741996<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Adenylosuccinate synthase]]		[[Category: Adenylosuccinate synthase]]
-	[[Category: Escherichia coli ~~k12~~]]	+	[[Category: Escherichia coli k-12]]
	[[Category: Fromm, H J.]]		[[Category: Fromm, H J.]]
	[[Category: Honzatko, R B.]]		[[Category: Honzatko, R B.]]

OCA at 07:58, 20 October 2012

OCA — Sat, 20 Oct 2012 07:58:42 GMT

←Older revision		Revision as of 07:58, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1kjx]] is a 1 chain structure ~~of [[Adenylosuccinate Synthetase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA].	+	[[1kjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KJX OCA].

	==See Also==		==See Also==