1kl8 - Revision history

OCA at 08:01, 3 April 2024

OCA — Wed, 03 Apr 2024 08:01:16 GMT

←Older revision		Revision as of 08:01, 3 April 2024
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	==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==		==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==
-	<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [https://en.wikipedia.org/wiki/~~Chick Chick~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [https://pdbe.org/1kl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [https://pdbe.org/1kl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~NXL1A_BUNMU NXL1A_BUNMU]~~] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) ~~nicotinic acetylcholine receptors~~. ~~Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site~~. ~~Blocks~~ the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:~~9305882~~</ref> <ref>PMID:~~9840221~~</ref> ~~[[https~~://www.uniprot.org/uniprot/ACHA7_CHICK ACHA7_CHICK]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.	+	[https://www.uniprot.org/uniprot/3L21A_BUNMU 3L21A_BUNMU] Binds with high affinity to muscular (tested on Torpedo marmorata, Kd=0.4 nM) and neuronal (tested on chimeric alpha-7/CHRNA7, Kd=0.95 nM) nicotinic acetylcholine receptor (nAChR) and inhibits acetylcholine from binding to the receptor, thereby impairing neuromuscular and neuronal transmission (PubMed:9305882). It also shows an activity on GABA(A) receptors (PubMed:16549768, PubMed:25634239). It antagonises GABA-activated currents with high potency when tested on primary hippocampal neurons (PubMed:25634239). It inhibits recombinantly expressed GABA(A) receptors composed of alpha-2-beta-2-gamma-2 (GABRA2-GABRB2-GABRG2) subunits with high potency (62.3% inhibition at 20 uM of toxin) (PubMed:25634239). It also shows a weaker inhibition on GABA(A) receptors composed of alpha-1-beta-2-gamma-2 (GABRA1-GABRB2-GABRG2) subunits, alpha-4-beta-2-gamma-2 (GABRA4-GABRB2-GABRG2) subunits, and alpha-5-beta-2-gamma-2 (GABRA5-GABRB2-GABRG2) subunits (PubMed:25634239). A very weak inhibition is also observed on GABA(A) receptor composed of alpha-1-beta-3-gamma-2 (GABRA1-GABRB3-GABRG2) (PubMed:26221036). It has also been shown to bind and inhibit recombinant GABA(A) receptor beta-3/GABRB3 subunit (Kd=about 50 nM) (PubMed:16549768). In addition, it blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM) (PubMed:9840221).<ref>PMID:16549768</ref> <ref>PMID:25634239</ref> <ref>PMID:9305882</ref> <ref>PMID:9840221</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kl8 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kl8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We report a new, higher resolution NMR structure of alpha-bungarotoxin that defines the structure-determining disulfide core and beta-sheet regions. We further report the NMR structure of the stoichiometric complex formed between alpha-bungarotoxin and a recombinantly expressed 19-mer peptide ((178)IPGKRTESFYECCKEPYPD(196)) derived from the alpha7 subunit of the chick neuronal nicotinic acetylcholine receptor. A comparison of these two structures reveals binding-induced stabilization of the flexible tip of finger II in alpha-bungarotoxin. The conformational rearrangements in the toxin create an extensive binding surface involving both sides of the alpha7 19-mer hairpin-like structure. At the contact zone, Ala(7), Ser(9), and Ile(11) in finger I and Arg(36), Lys(38), Val(39), and Val(40) in finger II of alpha-bungarotoxin interface with Phe(186), Tyr(187), Glu(188), and Tyr(194) in the alpha7 19-mer underscoring the importance of receptor aromatic residues as critical neurotoxin-binding determinants. Superimposing the structure of the complex onto that of the acetylcholine-binding protein (1I9B), a soluble homologue of the extracellular domain of the alpha7 receptor, places alpha-bungarotoxin at the peripheral surface of the inter-subunit interface occluding the agonist-binding site. The disulfide-rich core of alpha-bungarotoxin is suggested to be tilted in the direction of the membrane surface with finger II extending into the proposed ligand-binding cavity.
-
-	NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor.,Moise L, Piserchio A, Basus VJ, Hawrot E J Biol Chem. 2002 Apr 5;277(14):12406-17. Epub 2002 Jan 14. PMID:11790782<ref>PMID:11790782</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
-	[[Category: ~~Chick~~]]	+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Basus~~, V J~~]]	+	[[Category: Basus VJ]]
-	[[Category: Hawrot, E]]	+	[[Category: Hawrot E]]
-	[[Category: Moise, L]]	+	[[Category: Moise L]]
-	[[Category: Piserchio, A]]	+	[[Category: Piserchio A]]
-	~~[[Category: Alpha-bungarotoxin]]~~	+
-	~~[[Category: Alpha-neurotoxin]]~~	+
-	~~[[Category: Ligand-gated ion channel]]~~	+
-	~~[[Category: Nicotinic acetylcholine receptor alpha 7 subunit]]~~	+
-	~~[[Category: Nmr protein-protein interaction]]~~	+
-	~~[[Category: Protein-peptide complex]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 14:42, 27 October 2021

OCA — Wed, 27 Oct 2021 14:42:20 GMT

←Older revision		Revision as of 14:42, 27 October 2021
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	<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bunmu Bunmu~~] and [https://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [https://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</div></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bunmu~~]]	+	[[Category: Bungarus multicinctus]]
	[[Category: Chick]]		[[Category: Chick]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 08:48, 21 April 2021

OCA — Wed, 21 Apr 2021 08:48:39 GMT

←Older revision		Revision as of 08:48, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bunmu Bunmu] and [~~http~~://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunmu Bunmu] and [https://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [~~http~~://pdbe.org/1kl8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [https://pdbe.org/1kl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref> [[~~http~~://www.uniprot.org/uniprot/ACHA7_CHICK ACHA7_CHICK]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.	+	[[https://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref> [[https://www.uniprot.org/uniprot/ACHA7_CHICK ACHA7_CHICK]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:23, 17 July 2019

OCA — Wed, 17 Jul 2019 13:23:38 GMT

←Older revision		Revision as of 13:23, 17 July 2019
Line 1:		Line 1:

	==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==		==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==
-	<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1kl8' size='340' side='right'caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bunmu Bunmu] and [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bunmu Bunmu] and [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kl8_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kl8_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bungarotoxin 3D structures\|Bungarotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Bunmu]]		[[Category: Bunmu]]
	[[Category: Chick]]		[[Category: Chick]]
		+	[[Category: Large Structures]]
	[[Category: Basus, V J]]		[[Category: Basus, V J]]
	[[Category: Hawrot, E]]		[[Category: Hawrot, E]]

OCA at 07:15, 11 October 2017

OCA — Wed, 11 Oct 2017 07:15:15 GMT

←Older revision		Revision as of 07:15, 11 October 2017
Line 1:		Line 1:
		+
	==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==		==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==
	<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bungarus_multicinctus Bungarus multicinctus~~] and [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bunmu Bunmu] and [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://pdbe.org/1kl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://pdbe.org/1kl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1kl8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kl8 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Bungarotoxin\|Bungarotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bungarus multicinctus~~]]	+	[[Category: Bunmu]]
	[[Category: Chick]]		[[Category: Chick]]
	[[Category: Basus, V J]]		[[Category: Basus, V J]]

OCA at 07:51, 11 September 2015

OCA — Fri, 11 Sep 2015 07:51:49 GMT

←Older revision		Revision as of 07:51, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/~~Gallus_gallus Gallus gallus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Chick Chick]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://pdbe.org/1kl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1kl8" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
-	[[Category: ~~Gallus gallus~~]]	+	[[Category: Chick]]
	[[Category: Basus, V J]]		[[Category: Basus, V J]]
	[[Category: Hawrot, E]]		[[Category: Hawrot, E]]

OCA at 13:29, 25 December 2014

OCA — Thu, 25 Dec 2014 13:29:21 GMT

←Older revision		Revision as of 13:29, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NXL1A_BUNMU NXL1A_BUNMU]] Binds with high affinity to muscular and neuronal (alpha-7, alpha-8, and alpha-9) nicotinic acetylcholine receptors. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine only at the higher dose (4.2 uM).<ref>PMID:9305882</ref> <ref>PMID:9840221</ref> [[http://www.uniprot.org/uniprot/ACHA7_CHICK ACHA7_CHICK]] After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
-	[[Category: Basus, V J.]]	+	[[Category: Basus, V J]]
-	[[Category: Hawrot, E.]]	+	[[Category: Hawrot, E]]
-	[[Category: Moise, L.]]	+	[[Category: Moise, L]]
-	[[Category: Piserchio, A.]]	+	[[Category: Piserchio, A]]
	[[Category: Alpha-bungarotoxin]]		[[Category: Alpha-bungarotoxin]]
	[[Category: Alpha-neurotoxin]]		[[Category: Alpha-neurotoxin]]

OCA at 12:02, 28 September 2014

OCA — Sun, 28 Sep 2014 12:02:37 GMT

←Older revision		Revision as of 12:02, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1kl8.~~png~~\|~~left~~\|200px]]	+	==NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR==
		+	<StructureSection load='1kl8' size='340' side='right' caption='[[1kl8]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KL8 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HSL:HOMOSERINE+LACTONE'>HSL</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1abt\|1abt]], [[1idh\|1idh]], [[2abx\|2abx]], [[1i9b\|1i9b]], [[1hc9\|1hc9]], [[1jbd\|1jbd]], [[1kc4\|1kc4]], [[1kfh\|1kfh]], [[1haj\|1haj]], [[1bxp\|1bxp]], [[1ikc\|1ikc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1kl8 RCSB], [http://www.ebi.ac.uk/pdbsum/1kl8 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kl/1kl8_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We report a new, higher resolution NMR structure of alpha-bungarotoxin that defines the structure-determining disulfide core and beta-sheet regions. We further report the NMR structure of the stoichiometric complex formed between alpha-bungarotoxin and a recombinantly expressed 19-mer peptide ((178)IPGKRTESFYECCKEPYPD(196)) derived from the alpha7 subunit of the chick neuronal nicotinic acetylcholine receptor. A comparison of these two structures reveals binding-induced stabilization of the flexible tip of finger II in alpha-bungarotoxin. The conformational rearrangements in the toxin create an extensive binding surface involving both sides of the alpha7 19-mer hairpin-like structure. At the contact zone, Ala(7), Ser(9), and Ile(11) in finger I and Arg(36), Lys(38), Val(39), and Val(40) in finger II of alpha-bungarotoxin interface with Phe(186), Tyr(187), Glu(188), and Tyr(194) in the alpha7 19-mer underscoring the importance of receptor aromatic residues as critical neurotoxin-binding determinants. Superimposing the structure of the complex onto that of the acetylcholine-binding protein (1I9B), a soluble homologue of the extracellular domain of the alpha7 receptor, places alpha-bungarotoxin at the peripheral surface of the inter-subunit interface occluding the agonist-binding site. The disulfide-rich core of alpha-bungarotoxin is suggested to be tilted in the direction of the membrane surface with finger II extending into the proposed ligand-binding cavity.

-	~~{{STRUCTURE_1kl8\| PDB=1kl8 \| SCENE= }}~~	+	NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor.,Moise L, Piserchio A, Basus VJ, Hawrot E J Biol Chem. 2002 Apr 5;277(14):12406-17. Epub 2002 Jan 14. PMID:11790782<ref>PMID:11790782</ref>

-	~~===NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_11790782}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1kl8]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en~~.~~wikipedia.org~~/~~wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA].~~	+

	==See Also==		==See Also==
	*[[Bungarotoxin\|Bungarotoxin]]		*[[Bungarotoxin\|Bungarotoxin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:011790782<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]

OCA at 17:14, 20 October 2012

OCA — Sat, 20 Oct 2012 17:14:45 GMT

←Older revision		Revision as of 17:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1kl8]] is a 2 chain structure ~~of [[Bungarotoxin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA].	+	[[1kl8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KL8 OCA].

	==See Also==		==See Also==

OCA at 00:18, 27 July 2012

OCA — Fri, 27 Jul 2012 00:18:02 GMT

←Older revision		Revision as of 00:18, 27 July 2012
Line 1:		Line 1:
	[[Image:1kl8.png\|left\|200px]]		[[Image:1kl8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1kl8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1kl8\| PDB=1kl8 \| SCENE= }}		{{STRUCTURE_1kl8\| PDB=1kl8 \| SCENE= }}

	===NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR===		===NMR STRUCTURAL ANALYSIS OF THE COMPLEX FORMED BETWEEN ALPHA-BUNGAROTOXIN AND THE PRINCIPAL ALPHA-NEUROTOXIN BINDING SEQUENCE ON THE ALPHA7 SUBUNIT OF A NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11790782}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11790782 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11790782}}		{{ABSTRACT_PUBMED_11790782}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Bungarotoxin]]	+	*[[Bungarotoxin\|Bungarotoxin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11790782~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011790782</ref><references group="xtra"/>
	[[Category: Bungarus multicinctus]]		[[Category: Bungarus multicinctus]]
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
Line 38:		Line 27:
	[[Category: Nmr protein-protein interaction]]		[[Category: Nmr protein-protein interaction]]
	[[Category: Protein-peptide complex]]		[[Category: Protein-peptide complex]]
		+	[[Category: Toxin]]