1knz - Revision history

OCA at 07:26, 14 February 2024

OCA — Wed, 14 Feb 2024 07:26:25 GMT

←Older revision		Revision as of 07:26, 14 February 2024
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-	====	+	==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==
-	<StructureSection load='1knz' size='340' side='right'caption='[[1knz]]' scene=''>	+	<StructureSection load='1knz' size='340' side='right'caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A/SA11 Simian rotavirus A/SA11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [https://pdbe.org/1knz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [https://www.ebi.ac.uk/pdbsum/1knz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [https://pdbe.org/1knz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [https://www.ebi.ac.uk/pdbsum/1knz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NSP3_ROTS1 NSP3_ROTS1] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+
		+	==See Also==
		+	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Simian rotavirus A/SA11]]
		+	[[Category: Burley SK]]
		+	[[Category: Deo RC]]
		+	[[Category: Groft CM]]
		+	[[Category: Rajashankar KR]]

OCA at 06:33, 18 August 2021

OCA — Wed, 18 Aug 2021 06:33:44 GMT

←Older revision		Revision as of 06:33, 18 August 2021
Line 1:		Line 1:

-	==~~Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer~~==	+	====
-	<StructureSection load='1knz' size='340' side='right'caption='[[1knz]]~~, [[Resolution\|resolution]] 2.45Å~~' scene=''>	+	<StructureSection load='1knz' size='340' side='right'caption='[[1knz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=~~1KNZ~~ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=~~1KNZ~~ FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [~~http~~://pdbe.org/1knz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1knz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1knz ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [https://pdbe.org/1knz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [https://www.ebi.ac.uk/pdbsum/1knz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1knz ProSAT]</span></td></tr>
	</table>		</table>
-	== Function ==
-	[[http://www.uniprot.org/uniprot/NSP3_ROTS1 NSP3_ROTS1]] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 16:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as nonstructural protein 3 (NSP3) for translation of viral mRNAs. NSP3 binds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G. The X-ray structure of the NSP3 RNA binding domain bound to a rotaviral mRNA 3' end has been determined. NSP3 is a novel, heart-shaped homodimer with a medial RNA binding cleft. The homodimer is asymmetric, and contains two similar N-terminal segments plus two structurally different C-terminal segments that intertwine to create a tunnel enveloping the mRNA 3' end. Biophysical studies demonstrate high affinity binding leading to increased thermal stability and slow dissociation kinetics, consistent with NSP3 function.
-
-	Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer.,Deo RC, Groft CM, Rajashankar KR, Burley SK Cell. 2002 Jan 11;108(1):71-81. PMID:11792322<ref>PMID:11792322</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Simian 11 rotavirus]]~~	+	[[Category: Z-disk]]
-	~~[[Category: Burley, S K]]~~	+
-	~~[[Category: Deo, R C]]~~	+
-	~~[[Category: Groft, C M]]~~	+
-	~~[[Category: Rajashankar, K R]]~~	+
-	~~[[Category: Protein~~-~~ssrna complex]]~~	+
-	~~[[Category: Viral protein-rna complex~~]]	+

OCA at 12:11, 13 November 2019

OCA — Wed, 13 Nov 2019 12:11:49 GMT

←Older revision		Revision as of 12:11, 13 November 2019
Line 1:		Line 1:

	==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==		==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==
-	<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='1knz' size='340' side='right'caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nonstructural protein 3D structures\|Nonstructural protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Simian 11 rotavirus]]		[[Category: Simian 11 rotavirus]]
	[[Category: Burley, S K]]		[[Category: Burley, S K]]

OCA at 21:21, 24 January 2018

OCA — Wed, 24 Jan 2018 21:21:29 GMT

←Older revision		Revision as of 21:21, 24 January 2018
Line 1:		Line 1:
		+
	==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==		==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==
	<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://pdbe.org/1knz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://pdbe.org/1knz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1knz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1knz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1knz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Nonstructural protein\|Nonstructural protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:04, 10 February 2016

OCA — Wed, 10 Feb 2016 06:04:47 GMT

←Older revision		Revision as of 06:04, 10 February 2016
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1knz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:05, 11 September 2015

OCA — Fri, 11 Sep 2015 06:05:47 GMT

←Older revision		Revision as of 06:05, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Simian_rotavirus_a/sa11~~ Simian rotavirus ~~a/sa11~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_11_rotavirus Simian 11 rotavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://pdbe.org/1knz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~VN34_ROTS1 VN34_ROTS1~~]] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).	+	[[http://www.uniprot.org/uniprot/NSP3_ROTS1 NSP3_ROTS1]] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1knz" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Simian rotavirus ~~a/sa11~~]]	+	[[Category: Simian 11 rotavirus]]
	[[Category: Burley, S K]]		[[Category: Burley, S K]]
	[[Category: Deo, R C]]		[[Category: Deo, R C]]

OCA at 12:19, 25 December 2014

OCA — Thu, 25 Dec 2014 12:19:48 GMT

←Older revision		Revision as of 12:19, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VN34_ROTS1 VN34_ROTS1]] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Simian rotavirus a/sa11]]		[[Category: Simian rotavirus a/sa11]]
-	[[Category: Burley, S K.]]	+	[[Category: Burley, S K]]
-	[[Category: Deo, R C.]]	+	[[Category: Deo, R C]]
-	[[Category: Groft, C M.]]	+	[[Category: Groft, C M]]
-	[[Category: Rajashankar, K R.]]	+	[[Category: Rajashankar, K R]]
	[[Category: Protein-ssrna complex]]		[[Category: Protein-ssrna complex]]
	[[Category: Viral protein-rna complex]]		[[Category: Viral protein-rna complex]]

OCA at 14:21, 28 September 2014

OCA — Sun, 28 Sep 2014 14:21:52 GMT

←Older revision		Revision as of 14:21, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1knz.~~png\|left~~\|200px]]	+	==Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer==
		+	<StructureSection load='1knz' size='340' side='right' caption='[[1knz]], [[Resolution\|resolution]] 2.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KNZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1knz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1knz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1knz RCSB], [http://www.ebi.ac.uk/pdbsum/1knz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kn/1knz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rotaviruses, the cause of life-threatening diarrhea in humans and cattle, utilize a functional homolog of poly(A) binding protein (PABP) known as nonstructural protein 3 (NSP3) for translation of viral mRNAs. NSP3 binds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G. The X-ray structure of the NSP3 RNA binding domain bound to a rotaviral mRNA 3' end has been determined. NSP3 is a novel, heart-shaped homodimer with a medial RNA binding cleft. The homodimer is asymmetric, and contains two similar N-terminal segments plus two structurally different C-terminal segments that intertwine to create a tunnel enveloping the mRNA 3' end. Biophysical studies demonstrate high affinity binding leading to increased thermal stability and slow dissociation kinetics, consistent with NSP3 function.

-	~~{{STRUCTURE_1knz\| PDB=1knz \| SCENE= }}~~	+	Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer.,Deo RC, Groft CM, Rajashankar KR, Burley SK Cell. 2002 Jan 11;108(1):71-81. PMID:11792322<ref>PMID:11792322</ref>

-	~~===Recognition~~ of the ~~rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_11792322}}~~	+	==See Also==
-		+	*[[Nonstructural protein\|Nonstructural protein]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1knz~~]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:011792322<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Simian rotavirus a/sa11]]		[[Category: Simian rotavirus a/sa11]]
	[[Category: Burley, S K.]]		[[Category: Burley, S K.]]

OCA: Protected "1knz" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 19:47:57 GMT

Protected "1knz" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:47, 5 December 2012

OCA at 19:47, 5 December 2012

OCA — Wed, 05 Dec 2012 19:47:53 GMT

←Older revision		Revision as of 19:47, 5 December 2012
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-	{{Seed}}
	[[Image:1knz.png\|left\|200px]]		[[Image:1knz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1knz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1knz\| PDB=1knz \| SCENE= }}		{{STRUCTURE_1knz\| PDB=1knz \| SCENE= }}

	===Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer===		===Recognition of the rotavirus mRNA 3' consensus by an asymmetric NSP3 homodimer===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_11792322}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 11792322 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_11792322}}		{{ABSTRACT_PUBMED_11792322}}

	==About this Structure==		==About this Structure==
-	~~1KNZ~~ is a 12 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA].	+	[[1knz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KNZ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~11792322~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:011792322</ref><references group="xtra"/>
	[[Category: Simian rotavirus a/sa11]]		[[Category: Simian rotavirus a/sa11]]
	[[Category: Burley, S K.]]		[[Category: Burley, S K.]]
Line 30:		Line 18:
	[[Category: Rajashankar, K R.]]		[[Category: Rajashankar, K R.]]
	[[Category: Protein-ssrna complex]]		[[Category: Protein-ssrna complex]]
-		+	[[Category: Viral protein-rna complex]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 16:00:18 2009''~~	+