1l5a - Revision history

OCA at 07:29, 14 February 2024

OCA — Wed, 14 Feb 2024 07:29:54 GMT

←Older revision		Revision as of 07:29, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~666~~ "~~Bacillo virgola del Koch~~" ~~Trevisan 1884~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [~~http~~://pdbe.org/1l5a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [https://pdbe.org/1l5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [https://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9KTV9_VIBCH Q9KTV9_VIBCH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.
-
-	The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains.,Keating TA, Marshall CG, Walsh CT, Keating AE Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:12055621<ref>PMID:12055621</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1l5a" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Keating, A E~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: Keating~~, T A~~]]	+	[[Category: Keating AE]]
-	[[Category: ~~Marshall, C G~~]]	+	[[Category: Keating TA]]
-	[[Category: ~~Walsh, C T~~]]	+	[[Category: Marshall CG]]
-	[[Category: ~~Amide synthase]]~~	+	[[Category: Walsh CT]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Nonribosomal peptide synthetase]]~~	+
-	~~[[Category: Nrps condensation domain]]~~	+
-	~~[[Category: Vibriobactin~~]]	+

OCA at 07:54, 25 November 2020

OCA — Wed, 25 Nov 2020 07:54:56 GMT

←Older revision		Revision as of 07:54, 25 November 2020
Line 1:		Line 1:

	==Crystal Structure of VibH, an NRPS Condensation Enzyme==		==Crystal Structure of VibH, an NRPS Condensation Enzyme==
-	<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='1l5a' size='340' side='right'caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1L5A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Keating, A E]]		[[Category: Keating, A E]]
	[[Category: Keating, T A]]		[[Category: Keating, T A]]

OCA at 21:42, 24 January 2018

OCA — Wed, 24 Jan 2018 21:42:07 GMT

←Older revision		Revision as of 21:42, 24 January 2018
Line 1:		Line 1:
		+
	==Crystal Structure of VibH, an NRPS Condensation Enzyme==		==Crystal Structure of VibH, an NRPS Condensation Enzyme==
	<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5a ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:04, 8 February 2016

OCA — Mon, 08 Feb 2016 10:04:35 GMT

←Older revision		Revision as of 10:04, 8 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5a ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:28, 10 September 2015

OCA — Thu, 10 Sep 2015 10:28:54 GMT

←Older revision		Revision as of 10:28, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae Vibrio cholerae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 ~~Vibrio cholerae~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://pdbe.org/1l5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1l5a" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae~~]]	+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Keating, A E]]		[[Category: Keating, A E]]
	[[Category: Keating, T A]]		[[Category: Keating, T A]]

OCA at 16:02, 5 January 2015

OCA — Mon, 05 Jan 2015 16:02:38 GMT

←Older revision		Revision as of 16:02, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>		<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
-	[[Category: Keating, A E.]]	+	[[Category: Keating, A E]]
-	[[Category: Keating, T A.]]	+	[[Category: Keating, T A]]
-	[[Category: Marshall, C G.]]	+	[[Category: Marshall, C G]]
-	[[Category: Walsh, C T.]]	+	[[Category: Walsh, C T]]
	[[Category: Amide synthase]]		[[Category: Amide synthase]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]

OCA at 13:27, 28 September 2014

OCA — Sun, 28 Sep 2014 13:27:05 GMT

←Older revision		Revision as of 13:27, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1l5a.~~png\|left~~\|200px]]	+	==Crystal Structure of VibH, an NRPS Condensation Enzyme==
		+	<StructureSection load='1l5a' size='340' side='right' caption='[[1l5a]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L5A FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vibH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l5a RCSB], [http://www.ebi.ac.uk/pdbsum/1l5a PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5a_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nonribosomal peptide synthetases (NRPSs) are large, multidomain enzymes that biosynthesize medically important natural products. We report the crystal structure of the free-standing NRPS condensation (C) domain VibH, which catalyzes amide bond formation in the synthesis of vibriobactin, a Vibrio cholerae siderophore. Despite low sequence identity, NRPS condensation enzymes are structurally related to chloramphenicol acetyltransferase (CAT) and dihydrolipoamide acyltransferases. However, although the latter enzymes are homotrimers, VibH is a monomeric pseudodimer. The VibH structure is representative of both NRPS condensation and epimerization domains, as well as the condensation-variant cyclization domains, which are all expected to be monomers. Surprisingly, despite favorable positioning in the active site, a universally conserved histidine important in CAT and in other C domains is not critical for general base catalysis in VibH.

-	~~{{STRUCTURE_1l5a\| PDB=1l5a \| SCENE= }}~~	+	The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains.,Keating TA, Marshall CG, Walsh CT, Keating AE Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:12055621<ref>PMID:12055621</ref>

-	~~===Crystal Structure of VibH~~, ~~an NRPS Condensation Enzyme===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12055621}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1l5a]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vibrio_cholerae Vibrio cholerae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:012055621<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Keating, A E.]]		[[Category: Keating, A E.]]

OCA: Protected "1l5a" [edit=sysop:move=sysop]

OCA — Wed, 05 Dec 2012 20:15:56 GMT

Protected "1l5a" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:15, 5 December 2012

OCA at 20:15, 5 December 2012

OCA — Wed, 05 Dec 2012 20:15:54 GMT

←Older revision		Revision as of 20:15, 5 December 2012
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-	{{Seed}}
	[[Image:1l5a.png\|left\|200px]]		[[Image:1l5a.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1l5a", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1l5a\| PDB=1l5a \| SCENE= }}		{{STRUCTURE_1l5a\| PDB=1l5a \| SCENE= }}

	===Crystal Structure of VibH, an NRPS Condensation Enzyme===		===Crystal Structure of VibH, an NRPS Condensation Enzyme===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_12055621}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 12055621 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_12055621}}		{{ABSTRACT_PUBMED_12055621}}

	==About this Structure==		==About this Structure==
-	~~1L5A~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].	+	[[1l5a]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~12055621~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:012055621</ref><references group="xtra"/>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Keating, A E.]]		[[Category: Keating, A E.]]
Line 30:		Line 18:
	[[Category: Walsh, C T.]]		[[Category: Walsh, C T.]]
	[[Category: Amide synthase]]		[[Category: Amide synthase]]
		+	[[Category: Biosynthetic protein]]
	[[Category: Nonribosomal peptide synthetase]]		[[Category: Nonribosomal peptide synthetase]]
	[[Category: Nrps condensation domain]]		[[Category: Nrps condensation domain]]
	[[Category: Vibriobactin]]		[[Category: Vibriobactin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:11:36 2009''

OCA at 19:11, 16 February 2009

OCA — Mon, 16 Feb 2009 19:11:37 GMT

←Older revision		Revision as of 19:11, 16 February 2009
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	==About this Structure==		==About this Structure==
-	1L5A is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].	+	1L5A is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5A OCA].

	==Reference==		==Reference==
-	The structure of VibH represents nonribosomal peptide synthetase condensation, cyclization and epimerization domains., Keating TA, Marshall CG, Walsh CT, Keating AE, Nat Struct Biol. 2002 Jul;9(7):522-6. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/12055621 12055621]~~	+	<ref group="xtra">PMID:12055621</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Keating, A E.]]		[[Category: Keating, A E.]]
Line 35:		Line 34:
	[[Category: Vibriobactin]]		[[Category: Vibriobactin]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 2 11~~:43:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:11:36 2009''